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The map label for this gene is siaT [H]

Identifier: 227081922

GI number: 227081922

Start: 1874263

End: 1875546

Strand: Reverse

Name: siaT [H]

Synonym: VCM66_1715

Alternate gene names: 227081922

Gene position: 1875546-1874263 (Counterclockwise)

Preceding gene: 227081923

Following gene: 227081921

Centisome position: 64.84

GC content: 45.33

Gene sequence:

>1284_bases
ATGGTTGGTTCAATTTTTGGTTGGTTAGGATTGTTATTTGCGGGCATGCCAGTAGGTTTTTCACTTATTTTTGTTGCATT
AGCTTTCCTCATTCTCACCAATAGTACAGGGATTAATTTTGCGGCTCAGCAAATGCTGGGTGGGATCGACAACTTCACGT
TGCTAGCCGTTCCATTTTTTGTGTTAACTGGCCATCTAATGAATAGTGCCGGTATTACAGAAAGAATCTTCAACTTCGCG
AAGTCTCTCGTTGGCCATATTACGGGCAGCTTGGGTCATGTAAATATCATGGCAAGCTTGTTGTTCTCTGGAATGTCTGG
CTCAGCATTGGCAGATGCAGGTGGGTTAGGTCAGCTTGAAATCAAATCCATGCGTGATGCGAAGTATCACGATGATTTCG
CGGGCGGGCTGACAGCGGCATCTTGCATCATTGGTCCTCTGGTGCCGCCATCAGTACCACTAGTCATATACGGTGTGGTT
TCCAATACCTCTATTGGCGCATTATTTTTAGCGGGTGCGATACCTGGTTTGCTGTGTTGTATTGCCTTGATGGTGATGAG
TTATTTTATCTGCAAAAAGCGCGGATATATGACATTGCCTAAAGCGAGCAGACGTGAGCAATTTAAATCACTTAAAGAAG
CATTTTTATCGTTGCTGACACCAGTCATTATCATTGGTGGGATCTTCTCTGGTAAATTTACGCCAACTGAAGCGGCTGCT
GTTTCATCTCTCTACGCACTATTTTTAGGTACAGTTGTTTATAACACGCTCACGTTACAGGGGTTCATCGAAATTCTGAA
AGAAACGGTCAATACCACAGCCGTGGTTGCTCTGATGGTTATGGGCGTGACTGTTTTTGGGTGGATTGTGGCTCGCGAAC
AGTTACCACAGATGTTGGCTGATTATTTCTTAACAATCAGTGATAACCCACTCGTACTGCTTCTGTTGATCAACTTGTTG
CTCCTCTTCTTGGGCACGTTTATTGAGTCATTGGCCTTGCTGTTGCTGCTAGTTCCATTCTTGGTGCCAGTGGCTTCAGC
GGTGGGTATCGATCCTGTTCACTTCGGTGTTATGGCGATTCTTAACCTAATGATTGGCATTCTTACCCCACCAATGGGAA
TGGCTTTATATGTGGTTTCTCGAGTCGGAGATATCCCATTCCATACGCTTACGCGTGGTGTTTTACCACTACTGGTGCCG
CTATTCATCGTGTTGGCGCTGGTGGCGGTATTCCCTCAATTTACTCTGCTACTACCGGAATTATTCCTCGGATACGGACA
GTAG

Upstream 100 bases:

>100_bases
AGACAACTCGAAAAAATCTTTAACCTTATGAAGCTGCTACTGGGTGTTAGCTCATCTGCTTCATTAATTGATCAGCAAGT
AACGGAGAGGTAGTAGGACT

Downstream 100 bases:

>100_bases
TTGAACTAGCGAACTATAGAATTTAAAGGTAAACAAAATGAAAAAATTAACAGGTTTGATTGCTGCTCCACATACGCCTT
TCACAAAAGATAACAAGGTT

Product: Sialic acid TRAP transporter,large permease protein

Products: NA

Alternate protein names: N-acetylneuraminic acid permease; N-acetylneuraminic acid transporter; Neu5Ac permease [H]

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MVGSIFGWLGLLFAGMPVGFSLIFVALAFLILTNSTGINFAAQQMLGGIDNFTLLAVPFFVLTGHLMNSAGITERIFNFA
KSLVGHITGSLGHVNIMASLLFSGMSGSALADAGGLGQLEIKSMRDAKYHDDFAGGLTAASCIIGPLVPPSVPLVIYGVV
SNTSIGALFLAGAIPGLLCCIALMVMSYFICKKRGYMTLPKASRREQFKSLKEAFLSLLTPVIIIGGIFSGKFTPTEAAA
VSSLYALFLGTVVYNTLTLQGFIEILKETVNTTAVVALMVMGVTVFGWIVAREQLPQMLADYFLTISDNPLVLLLLINLL
LLFLGTFIESLALLLLLVPFLVPVASAVGIDPVHFGVMAILNLMIGILTPPMGMALYVVSRVGDIPFHTLTRGVLPLLVP
LFIVLALVAVFPQFTLLLPELFLGYGQ

Sequences:

>Translated_427_residues
MVGSIFGWLGLLFAGMPVGFSLIFVALAFLILTNSTGINFAAQQMLGGIDNFTLLAVPFFVLTGHLMNSAGITERIFNFA
KSLVGHITGSLGHVNIMASLLFSGMSGSALADAGGLGQLEIKSMRDAKYHDDFAGGLTAASCIIGPLVPPSVPLVIYGVV
SNTSIGALFLAGAIPGLLCCIALMVMSYFICKKRGYMTLPKASRREQFKSLKEAFLSLLTPVIIIGGIFSGKFTPTEAAA
VSSLYALFLGTVVYNTLTLQGFIEILKETVNTTAVVALMVMGVTVFGWIVAREQLPQMLADYFLTISDNPLVLLLLINLL
LLFLGTFIESLALLLLLVPFLVPVASAVGIDPVHFGVMAILNLMIGILTPPMGMALYVVSRVGDIPFHTLTRGVLPLLVP
LFIVLALVAVFPQFTLLLPELFLGYGQ
>Mature_427_residues
MVGSIFGWLGLLFAGMPVGFSLIFVALAFLILTNSTGINFAAQQMLGGIDNFTLLAVPFFVLTGHLMNSAGITERIFNFA
KSLVGHITGSLGHVNIMASLLFSGMSGSALADAGGLGQLEIKSMRDAKYHDDFAGGLTAASCIIGPLVPPSVPLVIYGVV
SNTSIGALFLAGAIPGLLCCIALMVMSYFICKKRGYMTLPKASRREQFKSLKEAFLSLLTPVIIIGGIFSGKFTPTEAAA
VSSLYALFLGTVVYNTLTLQGFIEILKETVNTTAVVALMVMGVTVFGWIVAREQLPQMLADYFLTISDNPLVLLLLINLL
LLFLGTFIESLALLLLLVPFLVPVASAVGIDPVHFGVMAILNLMIGILTPPMGMALYVVSRVGDIPFHTLTRGVLPLLVP
LFIVLALVAVFPQFTLLLPELFLGYGQ

Specific function: Part of the tripartite ATP-independent periplasmic (TRAP) transport system siaPT involved in the uptake of sialic acid [H]

COG id: COG1593

COG function: function code G; TRAP-type C4-dicarboxylate transport system, large permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the TRAP transporter large permease family [H]

Homologues:

Organism=Escherichia coli, GI48994950, Length=388, Percent_Identity=38.1443298969072, Blast_Score=246, Evalue=3e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010656
- InterPro:   IPR007387
- InterPro:   IPR004681 [H]

Pfam domain/function: PF06808 DctM; PF04290 DctQ [H]

EC number: NA

Molecular weight: Translated: 45457; Mature: 45457

Theoretical pI: Translated: 7.11; Mature: 7.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHCCCC
>Mature Secondary Structure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HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]