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The map label for this gene is nanA [H]

Identifier: 227081921

GI number: 227081921

Start: 1873329

End: 1874225

Strand: Reverse

Name: nanA [H]

Synonym: VCM66_1714

Alternate gene names: 227081921

Gene position: 1874225-1873329 (Counterclockwise)

Preceding gene: 227081922

Following gene: 227081920

Centisome position: 64.8

GC content: 43.14

Gene sequence:

>897_bases
ATGAAAAAATTAACAGGTTTGATTGCTGCTCCACATACGCCTTTCACAAAAGATAACAAGGTTAATTTTGCGGCGATTGA
CCAAATTGCTGAGCTTCTGATTGAACAAGGAGTGAAAGGTGCTTATGTCTGCGGAACGACAGGTGAGGGGATCCATTGCT
CTGTCGAAGAAAGAAAAGCAATTGCAGAACGTTGGGTGAAAGCCGTTGATGGGAAGTTAGATGTTATTTTACATACAGGC
GCTTTAAGTATTGTTGATACCATCAACTTGACAGAGCACGCTGAAACACTGGATATCTTTGCTACCTCCGCTATTGGCCC
ATGCTTTTTCAAACCGGGAAGTGTTGATGATTTAGTTGAGTATTGTGCTCAAGTTGCTGCCGCAGCTCCTTCAAAAGGTT
TTTATTACTACCACTCAGGAATGTCGGGCGTGAATCTAGATTTAGAGCAGTTCTTGATTAAAGGCGAACAGCGCATTCCT
AATCTTTATGGCGCAAAATTCAATAATGCTGATTTGTATGAATACCAGCGTTGCGTGCGAGTATCGAATCGCAAATTTGA
TATTCCATTTGGTGTGGATGAGTTTTTACCTGCTGGTTTAGCCGTCGGTGCGGTAGGCGCAGTTGGTAGCACTTATAACT
ATGCTGCTCCATTGTATTTGAAAATCATTGAAGCATTTAACCATGGTAAACATGATGAAGTCGCAGCGTTGATGGATAAA
GTGATTGCGATTATTCGAGTATTGGTCGAATACGGCGGTGTTGCGGCAGGTAAAGTTGCCATGCAGTTACATGGTATTGA
TGCTGGGGATCCCCGCTTACCTATTCGTTCATTGAATGACAAACAGAAAGCGGATGTGCTTGCGAAGATGCGTGATGCTG
GGTTCCTGTCGATCTAA

Upstream 100 bases:

>100_bases
GTGGCGGTATTCCCTCAATTTACTCTGCTACTACCGGAATTATTCCTCGGATACGGACAGTAGTTGAACTAGCGAACTAT
AGAATTTAAAGGTAAACAAA

Downstream 100 bases:

>100_bases
ATAAAGGCTAGGGAGGAATATTCATCCTCCCTAAAAGAAGATTTAAGAACAAATTATCATGATGATGTATTAATAGATCT
AGGTCTTTGGTACTTTTATG

Product: putative N-acetylneuraminate lyase

Products: NA

Alternate protein names: N-acetylneuraminate pyruvate-lyase; N-acetylneuraminic acid aldolase; Sialate lyase; Sialic acid aldolase; Sialic acid lyase [H]

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MKKLTGLIAAPHTPFTKDNKVNFAAIDQIAELLIEQGVKGAYVCGTTGEGIHCSVEERKAIAERWVKAVDGKLDVILHTG
ALSIVDTINLTEHAETLDIFATSAIGPCFFKPGSVDDLVEYCAQVAAAAPSKGFYYYHSGMSGVNLDLEQFLIKGEQRIP
NLYGAKFNNADLYEYQRCVRVSNRKFDIPFGVDEFLPAGLAVGAVGAVGSTYNYAAPLYLKIIEAFNHGKHDEVAALMDK
VIAIIRVLVEYGGVAAGKVAMQLHGIDAGDPRLPIRSLNDKQKADVLAKMRDAGFLSI

Sequences:

>Translated_298_residues
MKKLTGLIAAPHTPFTKDNKVNFAAIDQIAELLIEQGVKGAYVCGTTGEGIHCSVEERKAIAERWVKAVDGKLDVILHTG
ALSIVDTINLTEHAETLDIFATSAIGPCFFKPGSVDDLVEYCAQVAAAAPSKGFYYYHSGMSGVNLDLEQFLIKGEQRIP
NLYGAKFNNADLYEYQRCVRVSNRKFDIPFGVDEFLPAGLAVGAVGAVGSTYNYAAPLYLKIIEAFNHGKHDEVAALMDK
VIAIIRVLVEYGGVAAGKVAMQLHGIDAGDPRLPIRSLNDKQKADVLAKMRDAGFLSI
>Mature_298_residues
MKKLTGLIAAPHTPFTKDNKVNFAAIDQIAELLIEQGVKGAYVCGTTGEGIHCSVEERKAIAERWVKAVDGKLDVILHTG
ALSIVDTINLTEHAETLDIFATSAIGPCFFKPGSVDDLVEYCAQVAAAAPSKGFYYYHSGMSGVNLDLEQFLIKGEQRIP
NLYGAKFNNADLYEYQRCVRVSNRKFDIPFGVDEFLPAGLAVGAVGAVGSTYNYAAPLYLKIIEAFNHGKHDEVAALMDK
VIAIIRVLVEYGGVAAGKVAMQLHGIDAGDPRLPIRSLNDKQKADVLAKMRDAGFLSI

Specific function: Catalyzes the cleavage of N-acetylneuraminic acid (sialic acid) to form pyruvate and N-acetylmannosamine via a Schiff base intermediate [H]

COG id: COG0329

COG function: function code EM; Dihydrodipicolinate synthase/N-acetylneuraminate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHDPS family. NanA subfamily [H]

Homologues:

Organism=Homo sapiens, GI13540533, Length=303, Percent_Identity=39.9339933993399, Blast_Score=202, Evalue=2e-52,
Organism=Escherichia coli, GI1789620, Length=250, Percent_Identity=29.2, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI87082415, Length=306, Percent_Identity=26.797385620915, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI1786463, Length=228, Percent_Identity=27.1929824561404, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR002220
- InterPro:   IPR020625
- InterPro:   IPR005264 [H]

Pfam domain/function: PF00701 DHDPS [H]

EC number: =4.1.3.3 [H]

Molecular weight: Translated: 32221; Mature: 32221

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLTGLIAAPHTPFTKDNKVNFAAIDQIAELLIEQGVKGAYVCGTTGEGIHCSVEERKA
CCCHHCEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCEEECHHHHHH
IAERWVKAVDGKLDVILHTGALSIVDTINLTEHAETLDIFATSAIGPCFFKPGSVDDLVE
HHHHHHHHHCCCEEEEEECCCCHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
YCAQVAAAAPSKGFYYYHSGMSGVNLDLEQFLIKGEQRIPNLYGAKFNNADLYEYQRCVR
HHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHCHHHCCHHHCCCCCCCHHHHHHHHHH
VSNRKFDIPFGVDEFLPAGLAVGAVGAVGSTYNYAAPLYLKIIEAFNHGKHDEVAALMDK
HCCCEECCCCCHHHHHHCHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHH
VIAIIRVLVEYGGVAAGKVAMQLHGIDAGDPRLPIRSLNDKQKADVLAKMRDAGFLSI
HHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKKLTGLIAAPHTPFTKDNKVNFAAIDQIAELLIEQGVKGAYVCGTTGEGIHCSVEERKA
CCCHHCEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCEEECHHHHHH
IAERWVKAVDGKLDVILHTGALSIVDTINLTEHAETLDIFATSAIGPCFFKPGSVDDLVE
HHHHHHHHHCCCEEEEEECCCCHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
YCAQVAAAAPSKGFYYYHSGMSGVNLDLEQFLIKGEQRIPNLYGAKFNNADLYEYQRCVR
HHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHCHHHCCHHHCCCCCCCHHHHHHHHHH
VSNRKFDIPFGVDEFLPAGLAVGAVGAVGSTYNYAAPLYLKIIEAFNHGKHDEVAALMDK
HCCCEECCCCCHHHHHHCHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHH
VIAIIRVLVEYGGVAAGKVAMQLHGIDAGDPRLPIRSLNDKQKADVLAKMRDAGFLSI
HHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481431 [H]