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The map label for this gene is topA [H]

Identifier: 227081878

GI number: 227081878

Start: 1814352

End: 1816982

Strand: Reverse

Name: topA [H]

Synonym: VCM66_1670

Alternate gene names: 227081878

Gene position: 1816982-1814352 (Counterclockwise)

Preceding gene: 227081880

Following gene: 227081876

Centisome position: 62.82

GC content: 50.4

Gene sequence:

>2631_bases
ATGGGTAAATCACTCGTTATCGTCGAGTCGCCAGCCAAGGCTAAAACCATCAATAAGTACCTTGGTAAAGACTTTATCGT
GAAATCCAGTGTGGGACACGTGCGTGATCTGCCTACAGCAGGGCAAACGGCCACACCGACCGGTAAAGCGGCGGCAGCAT
CGACTAAGAAAGCATCTACTACTGATAAAGAGCAGCAGAAGCGTGAAAAAGAACGCGCAGCGCTGATCAAAAAGATGGGT
GTCGATCCGTATCACGGCTGGCAAGCCAATTACCAAATTCTGCCTGGTAAAGAGAAAGTGGTGGCTGAGCTGCAAAAGCT
CGCGAAAGACGCTGACAGCGTTTATCTCGCAACCGACTTGGACCGCGAGGGAGAAGCCATCGCTTGGCACCTTCGTGAGA
TCATCGGTGGCGATGAAAAGCGATATAAACGAGTGGTATTTAATGAAATCACCAAAAACGCCATTCAGCAGGCTTTTAAG
CAGCCTGGTGAACTGAATATGGATGGCGTTAACGCTCAACAAGCACGTCGTTTCATGGACCGCGTAGTGGGCTTTATGGT
CTCACCTCTGCTGTGGAAAAAAGTGGCGCGAGGCCTTTCCGCTGGTCGTGTACAGTCTGTCGCCGTGAAACTTCTGGTTG
AGCGTGAACGCGAAATCAAAGCGTTTGTTCCGGAAGAGTTTTGGGACATCAATGCCGATACCTTGACCGCAGACAAGCAA
GATTTTCGCCTGTTGGTGGCCCAAAAAGACGGTTTAGCGTTCAAGCCCGTGAACGAAACGGAAGCCTTGGCGGCGGTTGC
TGCGCTCAATAAAGCGCAGTACGAAGTGTGCAAACGCGAAGACAAACCGACCACCAGTAAACCGAGTGCGCCTTTTATTA
CGTCAACCCTGCAGCAAGCCGCGAGTACTCGCCTTGGCTATGGGGTGAAAAAGACCATGATGCTCGCTCAGCGTTTGTAT
GAAGCGGGTTACATCACCTATATGCGTACCGACTCAACCAACTTGAGTGCGGAAGCGGTTGAGAATCTGCGTGGCTACAT
CACCAAGCATTACGGTGAGGCGTATTTGCCAAGCCAGCCAAATGTGTATGGCAGCAAGCAAAATGCCCAAGAGGCGCACG
AAGCGATTCGTCCTTCGGATGTGACTGTCACCGCCGACGCTCTGGAAGGAATGGAAGCGGATGCACACAAGCTGTATGCC
CTGATTTGGAATCAGTTTGTGGCCTGTCAAATGACACCTGCGCAATACGATTCCACGACAGTCAGTGTAAAAGCGGCAGA
GTACACCCTGAAAGCGAAAGGTCGTATCCTCAAGTTTGATGGTTGGACTCGCGTACAGCGCCCGTTGGGTAAAAACGAAG
ATCAGATCCTGCCTCCGGTGAAGGTCGGTGATAGCCTAAAGCTCGTCAGCCTAGATCCGAAACAGCATTTTACCAAACCG
CCAGCACGTTACACGGAAGCGGCATTGGTGAAAGAGTTAGAAAAGCGTGGTATTGGTCGCCCATCGACGTATGCATCGAT
CATTTCCACCATTCAAGACCGTGGCTATGTGAAAGTCGATCAGCGCCGTTTCTTCGCGGAAAAAATGGGTGAAATTGTCA
CCGATCGTCTTGATGAGAATTTTGACGACTTGATGAACTACGACTTTACCGCGCGTATGGAAGAGAAACTCGACCAAATC
GCCGAAGGTGAAGTGAACTGGACCGCAGTACTGGATGAGTTCTTTGCTGATTTCAGTCGCGACCTCGAAACGGCGGAGCA
AGATGAAAGCTTAGGTGGCATGAAGCCTAATCACATCGTGATGACCAATATTTTGTGTCCGGCCTGCTCGCGTCCGATGG
GGATCCGCACGGCATCGACAGGGGTGTTTTTAGGCTGTTCTGGCTATGCATTACCGCCGAAAGAGCGCTGCAAAACCACC
ATCAACTTAGGTGACGAAGAAGGGGTGATCAACGTTCTCGAAGAGGATGTCGAAACCGCAGCACTGCGTGCTAAGAAGCG
TTGCCCAATTTGTGAAACGGCGATGGATGCGTATCTGATTGACGATAAGCGTAAGCTGCATGTTTGTGGTAACAATCCGA
ACTGTGAAGGCTTTATCGTTGAAGAAGGCGAGTTCAAAGTCAAAGGCTATGAAGGCCCAACCGTCGAGTGTGATAAATGT
GGCTCAGACATGGTGCTGAAAAATGGCCGCTTTGGTAAGTACATGGGTTGTACCAACGATGCTTGTAAAAATACGCGTAA
GATCCTGAAAAATGGCGAAGTTGCGCCACCGAAAGAAGAGCCAGTGCATTTCCCTGAACTGCCTTGTGAAAATTCGGATG
CGTATTTTGTGCTGCGTGATGGTGCATCTGGCTTGTTCTTTGCGGCGAGCAATTTCCCTAAATCGCGTGAAACGCGCGCG
CCTTTGGTGGAAGAGTTGAAACGTTTTGCTGAGCGTTTGCCAGAAAAATACCAGTATCTGACTTCTGCACCCGCACATGA
CCCTGATGGCTTACCTGCTGTGGTGCGCTTCTCTCGTAAAGAGAAAGAGCATTACGTGCGTACCGAGACAGAGGGTAAAC
CTTCCGGTTGGATGGCGGTTTACCAAGAGGGTAAATGGTTGGTCACGGATAAACGCAAAAACGCGAAATAA

Upstream 100 bases:

>100_bases
TGATCTTCTTGTTATCTGGCTCGATAGTAAAAAAAGAATTACATTTTAGAACGTCATGCGATTTAAATCGCGCCAAATGA
TGAAGGACAATTGAGTCAAT

Downstream 100 bases:

>100_bases
TCCGACTCATCCTTTGTTCAATCAAAATAAGACAGGCAGCCTCGCTGCCTTGTCTTATTTTTGTATCAGGCTTTGCGAAG
CAGTTCTGAGAAATACCAGA

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 876; Mature: 875

Protein sequence:

>876_residues
MGKSLVIVESPAKAKTINKYLGKDFIVKSSVGHVRDLPTAGQTATPTGKAAAASTKKASTTDKEQQKREKERAALIKKMG
VDPYHGWQANYQILPGKEKVVAELQKLAKDADSVYLATDLDREGEAIAWHLREIIGGDEKRYKRVVFNEITKNAIQQAFK
QPGELNMDGVNAQQARRFMDRVVGFMVSPLLWKKVARGLSAGRVQSVAVKLLVEREREIKAFVPEEFWDINADTLTADKQ
DFRLLVAQKDGLAFKPVNETEALAAVAALNKAQYEVCKREDKPTTSKPSAPFITSTLQQAASTRLGYGVKKTMMLAQRLY
EAGYITYMRTDSTNLSAEAVENLRGYITKHYGEAYLPSQPNVYGSKQNAQEAHEAIRPSDVTVTADALEGMEADAHKLYA
LIWNQFVACQMTPAQYDSTTVSVKAAEYTLKAKGRILKFDGWTRVQRPLGKNEDQILPPVKVGDSLKLVSLDPKQHFTKP
PARYTEAALVKELEKRGIGRPSTYASIISTIQDRGYVKVDQRRFFAEKMGEIVTDRLDENFDDLMNYDFTARMEEKLDQI
AEGEVNWTAVLDEFFADFSRDLETAEQDESLGGMKPNHIVMTNILCPACSRPMGIRTASTGVFLGCSGYALPPKERCKTT
INLGDEEGVINVLEEDVETAALRAKKRCPICETAMDAYLIDDKRKLHVCGNNPNCEGFIVEEGEFKVKGYEGPTVECDKC
GSDMVLKNGRFGKYMGCTNDACKNTRKILKNGEVAPPKEEPVHFPELPCENSDAYFVLRDGASGLFFAASNFPKSRETRA
PLVEELKRFAERLPEKYQYLTSAPAHDPDGLPAVVRFSRKEKEHYVRTETEGKPSGWMAVYQEGKWLVTDKRKNAK

Sequences:

>Translated_876_residues
MGKSLVIVESPAKAKTINKYLGKDFIVKSSVGHVRDLPTAGQTATPTGKAAAASTKKASTTDKEQQKREKERAALIKKMG
VDPYHGWQANYQILPGKEKVVAELQKLAKDADSVYLATDLDREGEAIAWHLREIIGGDEKRYKRVVFNEITKNAIQQAFK
QPGELNMDGVNAQQARRFMDRVVGFMVSPLLWKKVARGLSAGRVQSVAVKLLVEREREIKAFVPEEFWDINADTLTADKQ
DFRLLVAQKDGLAFKPVNETEALAAVAALNKAQYEVCKREDKPTTSKPSAPFITSTLQQAASTRLGYGVKKTMMLAQRLY
EAGYITYMRTDSTNLSAEAVENLRGYITKHYGEAYLPSQPNVYGSKQNAQEAHEAIRPSDVTVTADALEGMEADAHKLYA
LIWNQFVACQMTPAQYDSTTVSVKAAEYTLKAKGRILKFDGWTRVQRPLGKNEDQILPPVKVGDSLKLVSLDPKQHFTKP
PARYTEAALVKELEKRGIGRPSTYASIISTIQDRGYVKVDQRRFFAEKMGEIVTDRLDENFDDLMNYDFTARMEEKLDQI
AEGEVNWTAVLDEFFADFSRDLETAEQDESLGGMKPNHIVMTNILCPACSRPMGIRTASTGVFLGCSGYALPPKERCKTT
INLGDEEGVINVLEEDVETAALRAKKRCPICETAMDAYLIDDKRKLHVCGNNPNCEGFIVEEGEFKVKGYEGPTVECDKC
GSDMVLKNGRFGKYMGCTNDACKNTRKILKNGEVAPPKEEPVHFPELPCENSDAYFVLRDGASGLFFAASNFPKSRETRA
PLVEELKRFAERLPEKYQYLTSAPAHDPDGLPAVVRFSRKEKEHYVRTETEGKPSGWMAVYQEGKWLVTDKRKNAK
>Mature_875_residues
GKSLVIVESPAKAKTINKYLGKDFIVKSSVGHVRDLPTAGQTATPTGKAAAASTKKASTTDKEQQKREKERAALIKKMGV
DPYHGWQANYQILPGKEKVVAELQKLAKDADSVYLATDLDREGEAIAWHLREIIGGDEKRYKRVVFNEITKNAIQQAFKQ
PGELNMDGVNAQQARRFMDRVVGFMVSPLLWKKVARGLSAGRVQSVAVKLLVEREREIKAFVPEEFWDINADTLTADKQD
FRLLVAQKDGLAFKPVNETEALAAVAALNKAQYEVCKREDKPTTSKPSAPFITSTLQQAASTRLGYGVKKTMMLAQRLYE
AGYITYMRTDSTNLSAEAVENLRGYITKHYGEAYLPSQPNVYGSKQNAQEAHEAIRPSDVTVTADALEGMEADAHKLYAL
IWNQFVACQMTPAQYDSTTVSVKAAEYTLKAKGRILKFDGWTRVQRPLGKNEDQILPPVKVGDSLKLVSLDPKQHFTKPP
ARYTEAALVKELEKRGIGRPSTYASIISTIQDRGYVKVDQRRFFAEKMGEIVTDRLDENFDDLMNYDFTARMEEKLDQIA
EGEVNWTAVLDEFFADFSRDLETAEQDESLGGMKPNHIVMTNILCPACSRPMGIRTASTGVFLGCSGYALPPKERCKTTI
NLGDEEGVINVLEEDVETAALRAKKRCPICETAMDAYLIDDKRKLHVCGNNPNCEGFIVEEGEFKVKGYEGPTVECDKCG
SDMVLKNGRFGKYMGCTNDACKNTRKILKNGEVAPPKEEPVHFPELPCENSDAYFVLRDGASGLFFAASNFPKSRETRAP
LVEELKRFAERLPEKYQYLTSAPAHDPDGLPAVVRFSRKEKEHYVRTETEGKPSGWMAVYQEGKWLVTDKRKNAK

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=611, Percent_Identity=25.69558101473, Blast_Score=149, Evalue=1e-35,
Organism=Homo sapiens, GI4507635, Length=629, Percent_Identity=24.483306836248, Blast_Score=135, Evalue=2e-31,
Organism=Escherichia coli, GI1787529, Length=869, Percent_Identity=70.5408515535098, Blast_Score=1305, Evalue=0.0,
Organism=Escherichia coli, GI1788061, Length=562, Percent_Identity=24.5551601423488, Blast_Score=102, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI32563869, Length=519, Percent_Identity=25.4335260115607, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17555378, Length=511, Percent_Identity=24.6575342465753, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24585251, Length=630, Percent_Identity=25.8730158730159, Blast_Score=136, Evalue=7e-32,
Organism=Drosophila melanogaster, GI24640096, Length=491, Percent_Identity=25.6619144602851, Blast_Score=117, Evalue=4e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR013263
- InterPro:   IPR006171 [H]

Pfam domain/function: PF08272 Topo_Zn_Ribbon; PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 97850; Mature: 97719

Theoretical pI: Translated: 8.03; Mature: 8.03

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKSLVIVESPAKAKTINKYLGKDFIVKSSVGHVRDLPTAGQTATPTGKAAAASTKKAST
CCCCEEEEECCCHHHHHHHHHCCCCEEECCCCCHHCCCCCCCCCCCCCCHHHCCCCCCCC
TDKEQQKREKERAALIKKMGVDPYHGWQANYQILPGKEKVVAELQKLAKDADSVYLATDL
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCHHHHHHHHHHHHCCCCCEEEEECC
DREGEAIAWHLREIIGGDEKRYKRVVFNEITKNAIQQAFKQPGELNMDGVNAQQARRFMD
CCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
RVVGFMVSPLLWKKVARGLSAGRVQSVAVKLLVEREREIKAFVPEEFWDINADTLTADKQ
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCEEECCCC
DFRLLVAQKDGLAFKPVNETEALAAVAALNKAQYEVCKREDKPTTSKPSAPFITSTLQQA
CEEEEEEECCCCEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
ASTRLGYGVKKTMMLAQRLYEAGYITYMRTDSTNLSAEAVENLRGYITKHYGEAYLPSQP
HHHHHCCCHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
NVYGSKQNAQEAHEAIRPSDVTVTADALEGMEADAHKLYALIWNQFVACQMTPAQYDSTT
CCCCCCCCHHHHHHHCCCCCEEEEHHHHCCCCHHHHHHHHHHHHHHHEEEECCCCCCCCE
VSVKAAEYTLKAKGRILKFDGWTRVQRPLGKNEDQILPPVKVGDSLKLVSLDPKQHFTKP
EEEEEEEEEEECCCCEEEECCCHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCHHHCCCC
PARYTEAALVKELEKRGIGRPSTYASIISTIQDRGYVKVDQRRFFAEKMGEIVTDRLDEN
CHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHCCC
FDDLMNYDFTARMEEKLDQIAEGEVNWTAVLDEFFADFSRDLETAEQDESLGGMKPNHIV
HHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEE
MTNILCPACSRPMGIRTASTGVFLGCSGYALPPKERCKTTINLGDEEGVINVLEEDVETA
EEEEECCCCCCCCCCEECCCCEEEECCCCCCCCHHHCCEEECCCCCCCHHHHHHHHHHHH
ALRAKKRCPICETAMDAYLIDDKRKLHVCGNNPNCEGFIVEEGEFKVKGYEGPTVECDKC
HHHHHCCCCHHHHHHHHEEECCCCEEEEECCCCCCCEEEEECCCEEEECCCCCCCCHHCC
GSDMVLKNGRFGKYMGCTNDACKNTRKILKNGEVAPPKEEPVHFPELPCENSDAYFVLRD
CCCEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEC
GASGLFFAASNFPKSRETRAPLVEELKRFAERLPEKYQYLTSAPAHDPDGLPAVVRFSRK
CCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
EKEHYVRTETEGKPSGWMAVYQEGKWLVTDKRKNAK
HHHHHEECCCCCCCCCCEEEEECCCEEEECCCCCCC
>Mature Secondary Structure 
GKSLVIVESPAKAKTINKYLGKDFIVKSSVGHVRDLPTAGQTATPTGKAAAASTKKAST
CCCEEEEECCCHHHHHHHHHCCCCEEECCCCCHHCCCCCCCCCCCCCCHHHCCCCCCCC
TDKEQQKREKERAALIKKMGVDPYHGWQANYQILPGKEKVVAELQKLAKDADSVYLATDL
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCHHHHHHHHHHHHCCCCCEEEEECC
DREGEAIAWHLREIIGGDEKRYKRVVFNEITKNAIQQAFKQPGELNMDGVNAQQARRFMD
CCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
RVVGFMVSPLLWKKVARGLSAGRVQSVAVKLLVEREREIKAFVPEEFWDINADTLTADKQ
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCEEECCCC
DFRLLVAQKDGLAFKPVNETEALAAVAALNKAQYEVCKREDKPTTSKPSAPFITSTLQQA
CEEEEEEECCCCEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
ASTRLGYGVKKTMMLAQRLYEAGYITYMRTDSTNLSAEAVENLRGYITKHYGEAYLPSQP
HHHHHCCCHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
NVYGSKQNAQEAHEAIRPSDVTVTADALEGMEADAHKLYALIWNQFVACQMTPAQYDSTT
CCCCCCCCHHHHHHHCCCCCEEEEHHHHCCCCHHHHHHHHHHHHHHHEEEECCCCCCCCE
VSVKAAEYTLKAKGRILKFDGWTRVQRPLGKNEDQILPPVKVGDSLKLVSLDPKQHFTKP
EEEEEEEEEEECCCCEEEECCCHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCHHHCCCC
PARYTEAALVKELEKRGIGRPSTYASIISTIQDRGYVKVDQRRFFAEKMGEIVTDRLDEN
CHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHCCC
FDDLMNYDFTARMEEKLDQIAEGEVNWTAVLDEFFADFSRDLETAEQDESLGGMKPNHIV
HHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEE
MTNILCPACSRPMGIRTASTGVFLGCSGYALPPKERCKTTINLGDEEGVINVLEEDVETA
EEEEECCCCCCCCCCEECCCCEEEECCCCCCCCHHHCCEEECCCCCCCHHHHHHHHHHHH
ALRAKKRCPICETAMDAYLIDDKRKLHVCGNNPNCEGFIVEEGEFKVKGYEGPTVECDKC
HHHHHCCCCHHHHHHHHEEECCCCEEEEECCCCCCCEEEEECCCEEEECCCCCCCCHHCC
GSDMVLKNGRFGKYMGCTNDACKNTRKILKNGEVAPPKEEPVHFPELPCENSDAYFVLRD
CCCEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEC
GASGLFFAASNFPKSRETRAPLVEELKRFAERLPEKYQYLTSAPAHDPDGLPAVVRFSRK
CCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
EKEHYVRTETEGKPSGWMAVYQEGKWLVTDKRKNAK
HHHHHEECCCCCCCCCCEEEEECCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]