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The map label for this gene is purR

Identifier: 227081870

GI number: 227081870

Start: 1806655

End: 1807665

Strand: Direct

Name: purR

Synonym: VCM66_1661

Alternate gene names: 227081870

Gene position: 1806655-1807665 (Clockwise)

Preceding gene: 227081869

Following gene: 227081871

Centisome position: 62.46

GC content: 48.37

Gene sequence:

>1011_bases
ATGGCTACGATTAAAGATGTGGCGCGCCTTGCGGGCGTGTCAACCACTACCGTTTCTCACGTCATCAATAAAACTCGCTT
TGTGGCAGAAACCACCCAAGAGAAAGTGATGGAAGCCGTTAAGCAGCTGAACTACGCGCCAAGTGCCGTGGCACGCAGCT
TAAAGTGCAATACCACTCGCACGATTGGTATGTTAGTGACGCAATCTACTAACCTGTTTTTCTCGGAAGTTATCGACGGT
GTGGAAAGCTACTGTTACCGTCAAGGCTATACGCTGATCCTATGTAACACAGGCGGCATCTATGAAAAGCAACGCGATTA
TATCCGCATGCTGGCCGAAAAACGGGTTGATGGCATTTTGGTGATGTGCTCAGACCTGACCCAAGAGCTGCAAGATATGT
TGGATGCACACAAAGATATTCCAAAAGTGGTTATGGATTGGGGTCCAGAAACGTCGCACGCCGATAAAATCATCGATAAC
TCCGAAGAAGGCGGCTACCTCGCGACCAAATATCTCACCGACCGCGGTCACACTGAGATTGCATGTTTGAGTGGTCACTT
CGTCAAAGCCGCATGCCAAGAGCGCATTCAAGGTTTCCGTCGCGCGATGGCGGAGGCCAAGTTGACCGTCAATGAAGATT
GGATCTTGGAAGGTAACTTTGAGTGTGATACTGCCGTTTTAGCGGCGGATAAAATCATTGCGATGGACAAGCGCCCCACG
GCGGTATTCTGTTTCAACGATACGATGGCATTAGGTTTGATGAGCCGTTTGCAACAGAAAGGCATACGTATTCCAGAAGA
TATGTCTGTCATCGGTTACGATAATATCGAGTTAGCAGAATACTTCTCTCCACCACTGACCACAGTGCACCAACCTAAGC
GTCGTGTGGGTAAGAACGCGTTTGAAATTTTGCTAGAACGCATTAAAGACAAAGAACACGAGCGCCGAATTTTCGAAATG
CACCCAGAGATCGTAGAGCGCGACACGGTGAAAGACTTGACAAAATCCTAA

Upstream 100 bases:

>100_bases
GCTCGCACAGAGTGTGAAGATTCGCTCAGTCTCGCCTTACTTCTGCTGATTAAATCCACCGATGTTATTCGCTGGCAGCA
GTCATCGACAGGAACACTCT

Downstream 100 bases:

>100_bases
CTTTCTTGACAATTCATAACGCCTATATTTGAAAGCTTGTCACATAAATATGACAAAACATATGTAGTGCGAGTATTATT
AGTAGGGCTCATTCTCACAT

Product: transcriptional regulator, LacI family

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor

Number of amino acids: Translated: 336; Mature: 335

Protein sequence:

>336_residues
MATIKDVARLAGVSTTTVSHVINKTRFVAETTQEKVMEAVKQLNYAPSAVARSLKCNTTRTIGMLVTQSTNLFFSEVIDG
VESYCYRQGYTLILCNTGGIYEKQRDYIRMLAEKRVDGILVMCSDLTQELQDMLDAHKDIPKVVMDWGPETSHADKIIDN
SEEGGYLATKYLTDRGHTEIACLSGHFVKAACQERIQGFRRAMAEAKLTVNEDWILEGNFECDTAVLAADKIIAMDKRPT
AVFCFNDTMALGLMSRLQQKGIRIPEDMSVIGYDNIELAEYFSPPLTTVHQPKRRVGKNAFEILLERIKDKEHERRIFEM
HPEIVERDTVKDLTKS

Sequences:

>Translated_336_residues
MATIKDVARLAGVSTTTVSHVINKTRFVAETTQEKVMEAVKQLNYAPSAVARSLKCNTTRTIGMLVTQSTNLFFSEVIDG
VESYCYRQGYTLILCNTGGIYEKQRDYIRMLAEKRVDGILVMCSDLTQELQDMLDAHKDIPKVVMDWGPETSHADKIIDN
SEEGGYLATKYLTDRGHTEIACLSGHFVKAACQERIQGFRRAMAEAKLTVNEDWILEGNFECDTAVLAADKIIAMDKRPT
AVFCFNDTMALGLMSRLQQKGIRIPEDMSVIGYDNIELAEYFSPPLTTVHQPKRRVGKNAFEILLERIKDKEHERRIFEM
HPEIVERDTVKDLTKS
>Mature_335_residues
ATIKDVARLAGVSTTTVSHVINKTRFVAETTQEKVMEAVKQLNYAPSAVARSLKCNTTRTIGMLVTQSTNLFFSEVIDGV
ESYCYRQGYTLILCNTGGIYEKQRDYIRMLAEKRVDGILVMCSDLTQELQDMLDAHKDIPKVVMDWGPETSHADKIIDNS
EEGGYLATKYLTDRGHTEIACLSGHFVKAACQERIQGFRRAMAEAKLTVNEDWILEGNFECDTAVLAADKIIAMDKRPTA
VFCFNDTMALGLMSRLQQKGIRIPEDMSVIGYDNIELAEYFSPPLTTVHQPKRRVGKNAFEILLERIKDKEHERRIFEMH
PEIVERDTVKDLTKS

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1787948, Length=333, Percent_Identity=51.3513513513513, Blast_Score=363, Evalue=1e-102,
Organism=Escherichia coli, GI1790194, Length=329, Percent_Identity=47.112462006079, Blast_Score=330, Evalue=1e-91,
Organism=Escherichia coli, GI1790369, Length=312, Percent_Identity=32.6923076923077, Blast_Score=206, Evalue=1e-54,
Organism=Escherichia coli, GI1789202, Length=293, Percent_Identity=33.4470989761092, Blast_Score=166, Evalue=3e-42,
Organism=Escherichia coli, GI1788474, Length=333, Percent_Identity=32.7327327327327, Blast_Score=165, Evalue=5e-42,
Organism=Escherichia coli, GI1789068, Length=295, Percent_Identity=30.1694915254237, Blast_Score=159, Evalue=3e-40,
Organism=Escherichia coli, GI1787580, Length=321, Percent_Identity=28.9719626168224, Blast_Score=153, Evalue=2e-38,
Organism=Escherichia coli, GI1786540, Length=310, Percent_Identity=29.3548387096774, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI48994940, Length=322, Percent_Identity=26.3975155279503, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1786268, Length=305, Percent_Identity=25.5737704918033, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1790715, Length=333, Percent_Identity=24.6246246246246, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1787906, Length=327, Percent_Identity=25.0764525993884, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1789456, Length=320, Percent_Identity=26.875, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1790689, Length=320, Percent_Identity=22.5, Blast_Score=79, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PURR_VIBC3 (A5F7H0)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F7H0
- SMR:   A5F7H0
- STRING:   A5F7H0
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A1324
- eggNOG:   COG1609
- HOGENOM:   HBG753640
- OMA:   DWGPESS
- ProtClustDB:   CLSK874518
- BioCyc:   VCHO345073:VC0395_A1324-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_01277
- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761
- PRINTS:   PR00036
- SMART:   SM00354

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1; SSF47413 Lambda_like_DNA

EC number: NA

Molecular weight: Translated: 38002; Mature: 37871

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS00356 HTH_LACI_1; PS50932 HTH_LACI_2

Important sites: BINDING 73-73 BINDING 189-189 BINDING 220-220 BINDING 274-274

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATIKDVARLAGVSTTTVSHVINKTRFVAETTQEKVMEAVKQLNYAPSAVARSLKCNTTR
CCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCC
TIGMLVTQSTNLFFSEVIDGVESYCYRQGYTLILCNTGGIYEKQRDYIRMLAEKRVDGIL
EEEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCEE
VMCSDLTQELQDMLDAHKDIPKVVMDWGPETSHADKIIDNSEEGGYLATKYLTDRGHTEI
EHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHCCCCCCCEEEEEEECCCCCCEE
ACLSGHFVKAACQERIQGFRRAMAEAKLTVNEDWILEGNFECDTAVLAADKIIAMDKRPT
EEECCHHHHHHHHHHHHHHHHHHHHHCEEECCCEEEECCCCCCHHHHHHHHHEEECCCCC
AVFCFNDTMALGLMSRLQQKGIRIPEDMSVIGYDNIELAEYFSPPLTTVHQPKRRVGKNA
EEEEECCHHHHHHHHHHHHCCCCCCCCCEEECCCCCHHHHHCCCCCCHHCCHHHHHCHHH
FEILLERIKDKEHERRIFEMHPEIVERDTVKDLTKS
HHHHHHHHHCHHHHHHHHHHCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ATIKDVARLAGVSTTTVSHVINKTRFVAETTQEKVMEAVKQLNYAPSAVARSLKCNTTR
CCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCC
TIGMLVTQSTNLFFSEVIDGVESYCYRQGYTLILCNTGGIYEKQRDYIRMLAEKRVDGIL
EEEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCEE
VMCSDLTQELQDMLDAHKDIPKVVMDWGPETSHADKIIDNSEEGGYLATKYLTDRGHTEI
EHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHCCCCCCCEEEEEEECCCCCCEE
ACLSGHFVKAACQERIQGFRRAMAEAKLTVNEDWILEGNFECDTAVLAADKIIAMDKRPT
EEECCHHHHHHHHHHHHHHHHHHHHHCEEECCCEEEECCCCCCHHHHHHHHHEEECCCCC
AVFCFNDTMALGLMSRLQQKGIRIPEDMSVIGYDNIELAEYFSPPLTTVHQPKRRVGKNA
EEEEECCHHHHHHHHHHHHCCCCCCCCCEEECCCCCHHHHHCCCCCCHHCCHHHHHCHHH
FEILLERIKDKEHERRIFEMHPEIVERDTVKDLTKS
HHHHHHHHHCHHHHHHHHHHCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA