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The map label for this gene is metR [H]

Identifier: 227081855

GI number: 227081855

Start: 1784890

End: 1785807

Strand: Direct

Name: metR [H]

Synonym: VCM66_1646

Alternate gene names: 227081855

Gene position: 1784890-1785807 (Clockwise)

Preceding gene: 227081854

Following gene: 227081858

Centisome position: 61.71

GC content: 52.18

Gene sequence:

>918_bases
ATGATAGAGCTGAAACATTTGAAAACGCTGATCTCGCTACGCGATACCGGTTCACTGACCGCCACCGCGACCTCGTTGCA
TCTCACCCAGTCTGCGCTCTCGCATCAGATTAAAGACTTGGAAGCGCGAATTGGCGGGCAGCTTTTTTTGCGTAAAACAC
GCCCAGTGCGTTTTACCGCCGAAGGCGAAATTTTGCTGCGTTTGGCGGATGATATTTTGCCTAAGATGTCGCGCGCCGAG
AATGAACTGGCAAGCCTTAAAGAAGATGTGAATGGACGCCTGCACATGGCGATTGAGTGCCACTCCTGCTTTCAATGGCT
AATGCCCGCCCTGCGCGAATATCAGGTCAGCTGGCCAAATGTGGCGCTGGATTTTTCGTCCGGCTTTGGGTTTGAACCTC
TACCCGCGCTGTTAGCGGGGGAATTGGATCTGGTGATCACCTCGGATATTTTGCCACGCTCAGAAGTGCATTATGAGCCG
CTGTTTGATTTTGAAATGCGCCTTATCACCGCCACCAACCATCCATTGGCTGACAAAGCCGGTATTGAGCCCCAAGATTT
AGCCGATCAAACCATGCTCACCTATCCGGTGCAAAAGCAAAGATTGGATGTGGTGAAGCACTTTTTGCAACCCGCAGGCG
TTGAGCCCGCACGCTGGAAGCAAGCCGACAATACCTTGATGCTGGTACAAATGGTGTCGGCAGGATTAGGCGTCGCGGCA
CTACCCAATTGGGCAATCAGCGAGTTTTCACGCCAAGGGCTCATCACCAGTAAACCGTTAGGTAAAGGGCTATGGCGTCG
CTTGTTTGCCGCCACTCGCCATAGCGAAAAAGACAAACGCTACCTGCAAGCCTTTTTCGCTACCGCACGCCAACAATGCA
AAAGCCACCTTGATGGGATCAAGATGGCTTCACTTTAG

Upstream 100 bases:

>100_bases
AAGCAGGAAGGTTATAGCCATCTAGATGTTTACAGCTCCGAAAAATCGGCGTAACTTAGCTAAAAATTCATGTTGGCTTA
GGGATAAGTGAGGAGAGTTC

Downstream 100 bases:

>100_bases
GCATCACTACCGAGCATAAGCTTTGAACTGGTTGGTAAGAGGATCGTATTGATAACCCAGTACGTCTAACTTACCGACCA
GATGCTCAATATCCATTTCA

Product: transcriptional activator MetR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MIELKHLKTLISLRDTGSLTATATSLHLTQSALSHQIKDLEARIGGQLFLRKTRPVRFTAEGEILLRLADDILPKMSRAE
NELASLKEDVNGRLHMAIECHSCFQWLMPALREYQVSWPNVALDFSSGFGFEPLPALLAGELDLVITSDILPRSEVHYEP
LFDFEMRLITATNHPLADKAGIEPQDLADQTMLTYPVQKQRLDVVKHFLQPAGVEPARWKQADNTLMLVQMVSAGLGVAA
LPNWAISEFSRQGLITSKPLGKGLWRRLFAATRHSEKDKRYLQAFFATARQQCKSHLDGIKMASL

Sequences:

>Translated_305_residues
MIELKHLKTLISLRDTGSLTATATSLHLTQSALSHQIKDLEARIGGQLFLRKTRPVRFTAEGEILLRLADDILPKMSRAE
NELASLKEDVNGRLHMAIECHSCFQWLMPALREYQVSWPNVALDFSSGFGFEPLPALLAGELDLVITSDILPRSEVHYEP
LFDFEMRLITATNHPLADKAGIEPQDLADQTMLTYPVQKQRLDVVKHFLQPAGVEPARWKQADNTLMLVQMVSAGLGVAA
LPNWAISEFSRQGLITSKPLGKGLWRRLFAATRHSEKDKRYLQAFFATARQQCKSHLDGIKMASL
>Mature_305_residues
MIELKHLKTLISLRDTGSLTATATSLHLTQSALSHQIKDLEARIGGQLFLRKTRPVRFTAEGEILLRLADDILPKMSRAE
NELASLKEDVNGRLHMAIECHSCFQWLMPALREYQVSWPNVALDFSSGFGFEPLPALLAGELDLVITSDILPRSEVHYEP
LFDFEMRLITATNHPLADKAGIEPQDLADQTMLTYPVQKQRLDVVKHFLQPAGVEPARWKQADNTLMLVQMVSAGLGVAA
LPNWAISEFSRQGLITSKPLGKGLWRRLFAATRHSEKDKRYLQAFFATARQQCKSHLDGIKMASL

Specific function: Control of the last step in methionine biosynthesis; metR is a positive activator of the metA, metE and metH genes. It is also a negative regulator of its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790262, Length=303, Percent_Identity=56.7656765676568, Blast_Score=358, Evalue=1e-100,
Organism=Escherichia coli, GI157672245, Length=228, Percent_Identity=23.2456140350877, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1788887, Length=267, Percent_Identity=27.3408239700375, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI145693105, Length=274, Percent_Identity=24.4525547445255, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1790399, Length=257, Percent_Identity=27.6264591439689, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1786401, Length=266, Percent_Identity=25.187969924812, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787879, Length=249, Percent_Identity=26.1044176706827, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1790208, Length=244, Percent_Identity=25.4098360655738, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI145693193, Length=261, Percent_Identity=26.8199233716475, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787530, Length=261, Percent_Identity=22.6053639846743, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI1789506, Length=181, Percent_Identity=24.8618784530387, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1788297, Length=248, Percent_Identity=22.5806451612903, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI2367136, Length=237, Percent_Identity=22.7848101265823, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34223; Mature: 34223

Theoretical pI: Translated: 8.13; Mature: 8.13

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIELKHLKTLISLRDTGSLTATATSLHLTQSALSHQIKDLEARIGGQLFLRKTRPVRFTA
CCCHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCEEEEC
EGEILLRLADDILPKMSRAENELASLKEDVNGRLHMAIECHSCFQWLMPALREYQVSWPN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHHHHCCCCCC
VALDFSSGFGFEPLPALLAGELDLVITSDILPRSEVHYEPLFDFEMRLITATNHPLADKA
EEEECCCCCCCCCHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCC
GIEPQDLADQTMLTYPVQKQRLDVVKHFLQPAGVEPARWKQADNTLMLVQMVSAGLGVAA
CCCHHHHHCCHHEECCHHHHHHHHHHHHHCCCCCCCHHHHCCCCHHHHHHHHHCCCCHHH
LPNWAISEFSRQGLITSKPLGKGLWRRLFAATRHSEKDKRYLQAFFATARQQCKSHLDGI
CCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCC
KMASL
CCCCC
>Mature Secondary Structure
MIELKHLKTLISLRDTGSLTATATSLHLTQSALSHQIKDLEARIGGQLFLRKTRPVRFTA
CCCHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCEEEEC
EGEILLRLADDILPKMSRAENELASLKEDVNGRLHMAIECHSCFQWLMPALREYQVSWPN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHHHHCCCCCC
VALDFSSGFGFEPLPALLAGELDLVITSDILPRSEVHYEPLFDFEMRLITATNHPLADKA
EEEECCCCCCCCCHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCC
GIEPQDLADQTMLTYPVQKQRLDVVKHFLQPAGVEPARWKQADNTLMLVQMVSAGLGVAA
CCCHHHHHCCHHEECCHHHHHHHHHHHHHCCCCCCCHHHHCCCCHHHHHHHHHCCCCHHH
LPNWAISEFSRQGLITSKPLGKGLWRRLFAATRHSEKDKRYLQAFFATARQQCKSHLDGI
CCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCC
KMASL
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]