Click here to switch to the map view.

The map label for this gene is dltA [H]

Identifier: 227081732

GI number: 227081732

Start: 1636287

End: 1637957

Strand: Reverse

Name: dltA [H]

Synonym: VCM66_1519

Alternate gene names: 227081732

Gene position: 1637957-1636287 (Counterclockwise)

Preceding gene: 227081733

Following gene: 227081731

Centisome position: 56.63

GC content: 45.78

Gene sequence:

>1671_bases
ATGCCATACAATTCTGCGACAGATTTGTTACAGAGAGAGGGTTATGGTGTCGTGCATAACCAATTTATCAATAACATGGC
GATGTACTGCGAGACAAGACACTCTATTTTGGCGGCTATTCAGGCTAACCGACATGCCCCTGCATTATGGGTTAAACAGA
AAACCTACACCTATCAAGAGATGACCGATATGGCACTCTCATTGAGTGACTATTGGCATTTACAGGGCGTGCAGCGAGTT
GCGATCCTTTCGGTGCGCGATCTCGCGGCTTATTCTGCGATATGGGCGAGCTATTTGGGCGGTATGACTTATATTCCGCT
CAATGCGCGAGCAACAACAGAACAGATCCAAGAGACGCTGATCGCGACACAATGTGATTCCATCATGGTAGACGCGCAGC
AATTGAGTCGTTTGTCCTCCTTACTAGAGACTTGCATTGACCGATTACACATTTATGCACTGCCTGATGTGGATGTGGAA
CCGTTACGTCAGCAATATCCTCAGCACACTTTTCACACAGTGCAAATAACCGAACAAGATGTGGAATTACTGGTAGTGAA
ATACCATTTAGACAATGAACATGAGTACGCTTATATCATGCAAACCTCAGGTTCGACTGGTAAACCCAAACGTATTGCGG
TCAGCTACAGTAATTTGCATTGCTATATCAGTCAAATTGACAAACTATTTCCACTCAATGCGCAAGATCGAGTGGGGCAA
TATTCTGATTTGACTTTTGATCTTTCGGTACATGATATTTTTTACAGTTTGATCTCTGGTGCTTGTTTATATGTTGTGCC
TGAATTGGCGAAATTAAGTCCAGCAGAATTTATTCGTCACCATCAATTAACGGTATGGCTTTCTGTGCCTACTGTTATTG
AGTTGGCCCTACAAAGACAAACCTTAACCCCACACAGTTTACCTTCTCTGCGACTGAGTTTTTTCTGCGGACAAGCCTTG
CTGCATGATTTAGCCGAACAATGGCAACAGGCCACCCAGCAGCCGGTGATCAATCTGTATGGTCCAACAGAGTGCACGAT
TGCCGTTACCTATCATCGATTTGTCGCCCATTCAGGTATGGCATCGGTGCCGATTGGTCGTGCTTTTGAAGAGGAGTGTT
TAGCCATCATCAACGAACAAGGCGAGTTGATGCGCTTTGAATCTGCACCCGAGGGGTATCGCGGAGAATTACTTCTGTCG
GGCAAACAGTTAGTGAAGGGTTATCTGAATGATCCGCTGAATACGCAAAGTGCCTTTTTCCAGCACGAAGGGCGTCTTTG
GTATCGTTCAGGTGACATTGTCACTAAATCAAATGGCGTGCTGATTCATCTTGGGCGCCGCGATCACCAAGTGAAAATTG
CCGGACAACGGGTGGAATTAGAAGAGATTGAAACCGTTGTTCGCCGCGTGACTCAAGCGCACAGCGTGGCGATTGTGCCT
TGGCCTTTAAGTGAGTCGGGCTATGCCAGCGGCACGGTGGCGTTTGTGGATACCCATACCCAATGGCAACCAGATCTGTG
GTTGAGCCAATGCAAACAACAATTAAATCCGACTTTTGTGCCTAAGCGTTGGTATGCCATTGAACAATTACCAAGAAATG
CCAATGGCAAAACCGATATTAAAGCTTTACAACAACAATTAGCGAGTCAGACATATGAAACAAGCCATTGA

Upstream 100 bases:

>100_bases
TGATATGTCTATTGTCAATCGGTTGCTCGACAACTAGTTGCAATTATAGGTTGTAGACCTTCGCGCCATAGAAAGGAGGC
CGGGCGCTCGGTAGCTGACA

Downstream 100 bases:

>100_bases
AAATATCCTTATTGAGCGATTACAAACCAGTATTGAGGGTATTTCATCGATACTCACCAATAAATTTTTCGATGAGTTTG
ATTCCTTCTCTTTCATCGAT

Product: enterobactin synthetase component F-related protein

Products: NA

Alternate protein names: D-alanine-D-alanyl carrier protein ligase; DCL; D-alanine-activating enzyme; DAE [H]

Number of amino acids: Translated: 556; Mature: 555

Protein sequence:

>556_residues
MPYNSATDLLQREGYGVVHNQFINNMAMYCETRHSILAAIQANRHAPALWVKQKTYTYQEMTDMALSLSDYWHLQGVQRV
AILSVRDLAAYSAIWASYLGGMTYIPLNARATTEQIQETLIATQCDSIMVDAQQLSRLSSLLETCIDRLHIYALPDVDVE
PLRQQYPQHTFHTVQITEQDVELLVVKYHLDNEHEYAYIMQTSGSTGKPKRIAVSYSNLHCYISQIDKLFPLNAQDRVGQ
YSDLTFDLSVHDIFYSLISGACLYVVPELAKLSPAEFIRHHQLTVWLSVPTVIELALQRQTLTPHSLPSLRLSFFCGQAL
LHDLAEQWQQATQQPVINLYGPTECTIAVTYHRFVAHSGMASVPIGRAFEEECLAIINEQGELMRFESAPEGYRGELLLS
GKQLVKGYLNDPLNTQSAFFQHEGRLWYRSGDIVTKSNGVLIHLGRRDHQVKIAGQRVELEEIETVVRRVTQAHSVAIVP
WPLSESGYASGTVAFVDTHTQWQPDLWLSQCKQQLNPTFVPKRWYAIEQLPRNANGKTDIKALQQQLASQTYETSH

Sequences:

>Translated_556_residues
MPYNSATDLLQREGYGVVHNQFINNMAMYCETRHSILAAIQANRHAPALWVKQKTYTYQEMTDMALSLSDYWHLQGVQRV
AILSVRDLAAYSAIWASYLGGMTYIPLNARATTEQIQETLIATQCDSIMVDAQQLSRLSSLLETCIDRLHIYALPDVDVE
PLRQQYPQHTFHTVQITEQDVELLVVKYHLDNEHEYAYIMQTSGSTGKPKRIAVSYSNLHCYISQIDKLFPLNAQDRVGQ
YSDLTFDLSVHDIFYSLISGACLYVVPELAKLSPAEFIRHHQLTVWLSVPTVIELALQRQTLTPHSLPSLRLSFFCGQAL
LHDLAEQWQQATQQPVINLYGPTECTIAVTYHRFVAHSGMASVPIGRAFEEECLAIINEQGELMRFESAPEGYRGELLLS
GKQLVKGYLNDPLNTQSAFFQHEGRLWYRSGDIVTKSNGVLIHLGRRDHQVKIAGQRVELEEIETVVRRVTQAHSVAIVP
WPLSESGYASGTVAFVDTHTQWQPDLWLSQCKQQLNPTFVPKRWYAIEQLPRNANGKTDIKALQQQLASQTYETSH
>Mature_555_residues
PYNSATDLLQREGYGVVHNQFINNMAMYCETRHSILAAIQANRHAPALWVKQKTYTYQEMTDMALSLSDYWHLQGVQRVA
ILSVRDLAAYSAIWASYLGGMTYIPLNARATTEQIQETLIATQCDSIMVDAQQLSRLSSLLETCIDRLHIYALPDVDVEP
LRQQYPQHTFHTVQITEQDVELLVVKYHLDNEHEYAYIMQTSGSTGKPKRIAVSYSNLHCYISQIDKLFPLNAQDRVGQY
SDLTFDLSVHDIFYSLISGACLYVVPELAKLSPAEFIRHHQLTVWLSVPTVIELALQRQTLTPHSLPSLRLSFFCGQALL
HDLAEQWQQATQQPVINLYGPTECTIAVTYHRFVAHSGMASVPIGRAFEEECLAIINEQGELMRFESAPEGYRGELLLSG
KQLVKGYLNDPLNTQSAFFQHEGRLWYRSGDIVTKSNGVLIHLGRRDHQVKIAGQRVELEEIETVVRRVTQAHSVAIVPW
PLSESGYASGTVAFVDTHTQWQPDLWLSQCKQQLNPTFVPKRWYAIEQLPRNANGKTDIKALQQQLASQTYETSH

Specific function: Involved in the biosynthesis of D-alanyl-lipoteichoic acid (LTA). Catalyzes an ATP-dependent two-step reaction where it forms a high energy D-alanyl AMP intermediate and transfers the alanyl residues from AMP to Dcp [H]

COG id: COG1020

COG function: function code Q; Non-ribosomal peptide synthetase modules and related proteins

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. DltA subfamily [H]

Homologues:

Organism=Homo sapiens, GI45580730, Length=527, Percent_Identity=25.0474383301708, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI187761345, Length=532, Percent_Identity=23.8721804511278, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI187761343, Length=532, Percent_Identity=23.8721804511278, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI157311624, Length=538, Percent_Identity=20.631970260223, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI157311622, Length=538, Percent_Identity=20.631970260223, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI58082049, Length=541, Percent_Identity=21.0720887245841, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI42544132, Length=527, Percent_Identity=21.4421252371917, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI38505220, Length=361, Percent_Identity=19.9445983379501, Blast_Score=67, Evalue=6e-11,
Organism=Escherichia coli, GI1786801, Length=542, Percent_Identity=30.4428044280443, Blast_Score=168, Evalue=9e-43,
Organism=Escherichia coli, GI1788107, Length=539, Percent_Identity=22.0779220779221, Blast_Score=91, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17556356, Length=375, Percent_Identity=27.2, Blast_Score=85, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI32564422, Length=358, Percent_Identity=24.3016759776536, Blast_Score=72, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI32564420, Length=358, Percent_Identity=24.3016759776536, Blast_Score=72, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6319591, Length=412, Percent_Identity=27.4271844660194, Blast_Score=83, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6319699, Length=373, Percent_Identity=21.71581769437, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24648676, Length=391, Percent_Identity=26.5984654731458, Blast_Score=94, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010071
- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010072 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.1.1.13 [H]

Molecular weight: Translated: 63151; Mature: 63019

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPYNSATDLLQREGYGVVHNQFINNMAMYCETRHSILAAIQANRHAPALWVKQKTYTYQE
CCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHH
MTDMALSLSDYWHLQGVQRVAILSVRDLAAYSAIWASYLGGMTYIPLNARATTEQIQETL
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHH
IATQCDSIMVDAQQLSRLSSLLETCIDRLHIYALPDVDVEPLRQQYPQHTFHTVQITEQD
HHHCCHHHHCCHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHCCCCCEEEEEEECCC
VELLVVKYHLDNEHEYAYIMQTSGSTGKPKRIAVSYSNLHCYISQIDKLFPLNAQDRVGQ
EEEEEEEEEECCCCCEEEEEEECCCCCCCCEEEEEECCHHHEEHHHHHHCCCCCHHHCCC
YSDLTFDLSVHDIFYSLISGACLYVVPELAKLSPAEFIRHHQLTVWLSVPTVIELALQRQ
CCCEEEEEHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHCEEEEEEECHHHHHHHHHHC
TLTPHSLPSLRLSFFCGQALLHDLAEQWQQATQQPVINLYGPTECTIAVTYHRFVAHSGM
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEEEEEEEHHHHHHCCC
ASVPIGRAFEEECLAIINEQGELMRFESAPEGYRGELLLSGKQLVKGYLNDPLNTQSAFF
CCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCCEEECCHHHHHHHHCCCCCCHHHHH
QHEGRLWYRSGDIVTKSNGVLIHLGRRDHQVKIAGQRVELEEIETVVRRVTQAHSVAIVP
HCCCEEEEECCCEEEECCCEEEEECCCCCEEEEECCEECHHHHHHHHHHHHHHCCEEEEE
WPLSESGYASGTVAFVDTHTQWQPDLWLSQCKQQLNPTFVPKRWYAIEQLPRNANGKTDI
CCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCHHH
KALQQQLASQTYETSH
HHHHHHHHHHCCCCCC
>Mature Secondary Structure 
PYNSATDLLQREGYGVVHNQFINNMAMYCETRHSILAAIQANRHAPALWVKQKTYTYQE
CCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHH
MTDMALSLSDYWHLQGVQRVAILSVRDLAAYSAIWASYLGGMTYIPLNARATTEQIQETL
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHH
IATQCDSIMVDAQQLSRLSSLLETCIDRLHIYALPDVDVEPLRQQYPQHTFHTVQITEQD
HHHCCHHHHCCHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHCCCCCEEEEEEECCC
VELLVVKYHLDNEHEYAYIMQTSGSTGKPKRIAVSYSNLHCYISQIDKLFPLNAQDRVGQ
EEEEEEEEEECCCCCEEEEEEECCCCCCCCEEEEEECCHHHEEHHHHHHCCCCCHHHCCC
YSDLTFDLSVHDIFYSLISGACLYVVPELAKLSPAEFIRHHQLTVWLSVPTVIELALQRQ
CCCEEEEEHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHCEEEEEEECHHHHHHHHHHC
TLTPHSLPSLRLSFFCGQALLHDLAEQWQQATQQPVINLYGPTECTIAVTYHRFVAHSGM
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEEEEEEEHHHHHHCCC
ASVPIGRAFEEECLAIINEQGELMRFESAPEGYRGELLLSGKQLVKGYLNDPLNTQSAFF
CCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCCEEECCHHHHHHHHCCCCCCHHHHH
QHEGRLWYRSGDIVTKSNGVLIHLGRRDHQVKIAGQRVELEEIETVVRRVTQAHSVAIVP
HCCCEEEEECCCEEEECCCEEEEECCCCCEEEEECCEECHHHHHHHHHHHHHHCCEEEEE
WPLSESGYASGTVAFVDTHTQWQPDLWLSQCKQQLNPTFVPKRWYAIEQLPRNANGKTDI
CCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCHHH
KALQQQLASQTYETSH
HHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA