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The map label for this gene is ligA-2 [H]

Identifier: 227081697

GI number: 227081697

Start: 1602710

End: 1603558

Strand: Reverse

Name: ligA-2 [H]

Synonym: VCM66_1483

Alternate gene names: 227081697

Gene position: 1603558-1602710 (Counterclockwise)

Preceding gene: 227081698

Following gene: 227081693

Centisome position: 55.44

GC content: 49.82

Gene sequence:

>849_bases
ATGCTTATACGACTGACGCTCACCGCTGCCGCGATCATGCTGGCCCAGCAAACGCTGGCCAATGATGGGCCGCTGGCTTT
TATTCCAATTACTTACGCCAACAACTACCAACACGGCATTGATATTTTCGATTACTGGAAAAGTGAGAAGCTTGACGGTA
TCCGCGCGATTTGGACGGGGAAACAACTGGTCACTCGTAACGGTAACCCTATTGCTGCCCCCGCTTGGTTTACCGAGTCG
TTACCCTCTTATTCACTCGAAGGTGAATTGTGGGCAGGGCGCGGCAATTTCTCACTGGTACAGCAAACGGTTTTAGATAG
CCAACCGGCTGACTCCGCATGGCGCAAAATCAGCCTAATGCTGTTCGATATGCCTGATGCGGCGGGCGATTACAGTAAGC
GCTATTACAACCTCATCCATTTGGTGAATAGCCTTGATCTCAAGCATATCCGATATGTCGAACACACCCCGATAAAATCT
GAGGCGGAACTGCTTTCCTACCTTGATAACATCAGTGAGAAATCGGGGGAAGGGGTAATGCTGCGCAAAATCAGCGCTCG
CTATCAGGCAGGGCGAAGTAATGATTTGTTGAAGCTGAAAAGGCATCAGGATGCAGAAGCAACCGTGATTGGTTATCGGC
TCGGCATGGGAAAATACAAAGGTAAAATGGGCTCTATGCTGGTTCGGACCGCAGAGGGGCTAGAATTTTATATCGGCAGT
GGATTTAGTGATGTCGAGCGCGCCGAGCCGCCCAAGATTGGTAGTGTTATCACCTACCGTTACAACGGACTTACAACGGA
AGGCAAACCAAGGTTTGCCCGTTTTGTTCGGGTCAGAGAAAACTATTGA

Upstream 100 bases:

>100_bases
ACGAGATTTAATGAACTGTTTCGCATCGTGAATGCTTCACCCTTCCATACATCAGCGTCCCTAATGACAATGCTGACGCA
TTCTCATTAGGGACGTGACC

Downstream 100 bases:

>100_bases
GGCAAGCTACTGAGCTTGCCCAACCGTGATACGACAACGCGCAAACAGCCGCTGCCAATATTGGGCATGCTGCTGAGTCG
CAAGACGGAAAGTTTGATGG

Product: DNA ligase

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [ATP] [H]

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MLIRLTLTAAAIMLAQQTLANDGPLAFIPITYANNYQHGIDIFDYWKSEKLDGIRAIWTGKQLVTRNGNPIAAPAWFTES
LPSYSLEGELWAGRGNFSLVQQTVLDSQPADSAWRKISLMLFDMPDAAGDYSKRYYNLIHLVNSLDLKHIRYVEHTPIKS
EAELLSYLDNISEKSGEGVMLRKISARYQAGRSNDLLKLKRHQDAEATVIGYRLGMGKYKGKMGSMLVRTAEGLEFYIGS
GFSDVERAEPPKIGSVITYRYNGLTTEGKPRFARFVRVRENY

Sequences:

>Translated_282_residues
MLIRLTLTAAAIMLAQQTLANDGPLAFIPITYANNYQHGIDIFDYWKSEKLDGIRAIWTGKQLVTRNGNPIAAPAWFTES
LPSYSLEGELWAGRGNFSLVQQTVLDSQPADSAWRKISLMLFDMPDAAGDYSKRYYNLIHLVNSLDLKHIRYVEHTPIKS
EAELLSYLDNISEKSGEGVMLRKISARYQAGRSNDLLKLKRHQDAEATVIGYRLGMGKYKGKMGSMLVRTAEGLEFYIGS
GFSDVERAEPPKIGSVITYRYNGLTTEGKPRFARFVRVRENY
>Mature_282_residues
MLIRLTLTAAAIMLAQQTLANDGPLAFIPITYANNYQHGIDIFDYWKSEKLDGIRAIWTGKQLVTRNGNPIAAPAWFTES
LPSYSLEGELWAGRGNFSLVQQTVLDSQPADSAWRKISLMLFDMPDAAGDYSKRYYNLIHLVNSLDLKHIRYVEHTPIKS
EAELLSYLDNISEKSGEGVMLRKISARYQAGRSNDLLKLKRHQDAEATVIGYRLGMGKYKGKMGSMLVRTAEGLEFYIGS
GFSDVERAEPPKIGSVITYRYNGLTTEGKPRFARFVRVRENY

Specific function: Catalyzes efficient strand joining on a single nicked DNA [H]

COG id: COG1793

COG function: function code L; ATP-dependent DNA ligase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent DNA ligase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012310
- InterPro:   IPR016059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF01068 DNA_ligase_A_M [H]

EC number: =6.5.1.1 [H]

Molecular weight: Translated: 31816; Mature: 31816

Theoretical pI: Translated: 9.52; Mature: 9.52

Prosite motif: PS00333 DNA_LIGASE_A2 ; PS50160 DNA_LIGASE_A3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIRLTLTAAAIMLAQQTLANDGPLAFIPITYANNYQHGIDIFDYWKSEKLDGIRAIWTG
CEEEEEHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCHHHHHHCCCCCCCEEEEECC
KQLVTRNGNPIAAPAWFTESLPSYSLEGELWAGRGNFSLVQQTVLDSQPADSAWRKISLM
CEEEECCCCEEECCHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHCCCCCCHHHHEEEEE
LFDMPDAAGDYSKRYYNLIHLVNSLDLKHIRYVEHTPIKSEAELLSYLDNISEKSGEGVM
EEECCCCCCHHHHHHHHHHHHHHHCCHHHEEEECCCCCCHHHHHHHHHHHHHHCCCCCEE
LRKISARYQAGRSNDLLKLKRHQDAEATVIGYRLGMGKYKGKMGSMLVRTAEGLEFYIGS
EEEHHHHHCCCCCCCEEEEECCCCCCEEEEEEEECCCHHCCHHHHHHHHHHCCEEEEECC
GFSDVERAEPPKIGSVITYRYNGLTTEGKPRFARFVRVRENY
CCCHHHHCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MLIRLTLTAAAIMLAQQTLANDGPLAFIPITYANNYQHGIDIFDYWKSEKLDGIRAIWTG
CEEEEEHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCHHHHHHCCCCCCCEEEEECC
KQLVTRNGNPIAAPAWFTESLPSYSLEGELWAGRGNFSLVQQTVLDSQPADSAWRKISLM
CEEEECCCCEEECCHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHCCCCCCHHHHEEEEE
LFDMPDAAGDYSKRYYNLIHLVNSLDLKHIRYVEHTPIKSEAELLSYLDNISEKSGEGVM
EEECCCCCCHHHHHHHHHHHHHHHCCHHHEEEECCCCCCHHHHHHHHHHHHHHCCCCCEE
LRKISARYQAGRSNDLLKLKRHQDAEATVIGYRLGMGKYKGKMGSMLVRTAEGLEFYIGS
EEEHHHHHCCCCCCCEEEEECCCCCCEEEEEEEECCCHHCCHHHHHHHHHHCCEEEEECC
GFSDVERAEPPKIGSVITYRYNGLTTEGKPRFARFVRVRENY
CCCHHHHCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8550458; 7542800; 9060431 [H]