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The map label for this gene is fdhB [H]

Identifier: 227081670

GI number: 227081670

Start: 1572155

End: 1572763

Strand: Reverse

Name: fdhB [H]

Synonym: VCM66_1454

Alternate gene names: 227081670

Gene position: 1572763-1572155 (Counterclockwise)

Preceding gene: 227081671

Following gene: 227081669

Centisome position: 54.37

GC content: 53.04

Gene sequence:

>609_bases
ATGGCAAGAATGAAATTCCTGTGTGACACCAAACGCTGTATCGAATGTAACGGCTGTGTCACGGCCTGTAAAAATGAAAA
CGATGATGCGCTGGAATGGGGCATCCAGCGTCGCCGCGTAGTCACGCTCAACGATGGTGAGCCGGGTGAAAACTCGATTT
CCGTGGCTTGCATGCACTGTACCGATGCGCCTTGTATGGCGGTGTGTCCAGCAGACTGCTTTGTCCATACCGAAGATGGC
ATTGTGCTGCACAACAAAGATCTTTGTATCGGTTGTGGTTACTGCTTGTTTGCTTGTCCGTTTGGTGCGCCGCAGTTTCC
GAAACAGGCGGCGTTTGGTGAGCGCGGTAAGATGGACAAATGTACCTTCTGTGCTGGCGGCCCTGAAACAGAGCCGGGTT
CGGAAGAGGAACGTCGTAAATACGGAGCCAACCGTATTGCGGAAGGCAAACTGCCCATGTGCGCTTCTCTCTGTTCTACC
AAAGCACTGCTAGCGGGGGATGCGGAGAAAATCTCCGATATTTTCCGTCAACGCGTAGTAGAACGCGGTGCGAAAGGTGC
CGGTTGGACGAACGGTGAAGATCTCGCCTATGACGCAACTCGGAGTTAA

Upstream 100 bases:

>100_bases
CGGCAAACACCGCCACAACCTACGGCTACGATCCTGTCACCTTGATGCAAGAAACCAAAGTCACCCTCTGTAACATTCGT
AAAGCGTAAGGAGCTTACCC

Downstream 100 bases:

>100_bases
GTGGCTATGAGACAACAACTTTCTCGCGCCACTCGTTTTGTGCTGCCTTTATTGGCAGCACTGCTGTGCTGGTTGGCTCA
ACCCGTGATGGCAGAAGAGA

Product: formate dehydrogenase, iron-sulfur subunit

Products: CO2; NADH

Alternate protein names: NA

Number of amino acids: Translated: 202; Mature: 201

Protein sequence:

>202_residues
MARMKFLCDTKRCIECNGCVTACKNENDDALEWGIQRRRVVTLNDGEPGENSISVACMHCTDAPCMAVCPADCFVHTEDG
IVLHNKDLCIGCGYCLFACPFGAPQFPKQAAFGERGKMDKCTFCAGGPETEPGSEEERRKYGANRIAEGKLPMCASLCST
KALLAGDAEKISDIFRQRVVERGAKGAGWTNGEDLAYDATRS

Sequences:

>Translated_202_residues
MARMKFLCDTKRCIECNGCVTACKNENDDALEWGIQRRRVVTLNDGEPGENSISVACMHCTDAPCMAVCPADCFVHTEDG
IVLHNKDLCIGCGYCLFACPFGAPQFPKQAAFGERGKMDKCTFCAGGPETEPGSEEERRKYGANRIAEGKLPMCASLCST
KALLAGDAEKISDIFRQRVVERGAKGAGWTNGEDLAYDATRS
>Mature_201_residues
ARMKFLCDTKRCIECNGCVTACKNENDDALEWGIQRRRVVTLNDGEPGENSISVACMHCTDAPCMAVCPADCFVHTEDGI
VLHNKDLCIGCGYCLFACPFGAPQFPKQAAFGERGKMDKCTFCAGGPETEPGSEEERRKYGANRIAEGKLPMCASLCSTK
ALLAGDAEKISDIFRQRVVERGAKGAGWTNGEDLAYDATRS

Specific function: This chain is an electron transfer unit containing 18 cysteine residues, 16 of which occur in four clusters [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787872, Length=177, Percent_Identity=33.8983050847458, Blast_Score=96, Evalue=1e-21,
Organism=Escherichia coli, GI1787122, Length=177, Percent_Identity=32.7683615819209, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI1789370, Length=198, Percent_Identity=27.7777777777778, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI226510944, Length=194, Percent_Identity=31.9587628865979, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1790326, Length=200, Percent_Identity=29.5, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1787749, Length=214, Percent_Identity=28.9719626168224, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI2367345, Length=199, Percent_Identity=31.6582914572864, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1788811, Length=123, Percent_Identity=31.7073170731707, Blast_Score=68, Evalue=5e-13,
Organism=Escherichia coli, GI2367245, Length=174, Percent_Identity=31.0344827586207, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1789079, Length=101, Percent_Identity=38.6138613861386, Blast_Score=64, Evalue=6e-12,
Organism=Escherichia coli, GI87082114, Length=91, Percent_Identity=37.3626373626374, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR000813 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: 1.2.1.2

Molecular weight: Translated: 21910; Mature: 21779

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

8.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
11.9 %Cys+Met (Translated Protein)
9.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
11.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARMKFLCDTKRCIECNGCVTACKNENDDALEWGIQRRRVVTLNDGEPGENSISVACMHC
CCCCCHHHCCCCEEECCCCEEECCCCCCHHHHHCCCCCEEEEECCCCCCCCCEEEEEEEC
TDAPCMAVCPADCFVHTEDGIVLHNKDLCIGCGYCLFACPFGAPQFPKQAAFGERGKMDK
CCCCEEEECCCCCEEECCCCEEEECCCEEEECCHHHHCCCCCCCCCCCHHCCCCCCCCCC
CTFCAGGPETEPGSEEERRKYGANRIAEGKLPMCASLCSTKALLAGDAEKISDIFRQRVV
EEEECCCCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
ERGAKGAGWTNGEDLAYDATRS
HCCCCCCCCCCCCCEECCCCCC
>Mature Secondary Structure 
ARMKFLCDTKRCIECNGCVTACKNENDDALEWGIQRRRVVTLNDGEPGENSISVACMHC
CCCCHHHCCCCEEECCCCEEECCCCCCHHHHHCCCCCEEEEECCCCCCCCCEEEEEEEC
TDAPCMAVCPADCFVHTEDGIVLHNKDLCIGCGYCLFACPFGAPQFPKQAAFGERGKMDK
CCCCEEEECCCCCEEECCCCEEEECCCEEEECCHHHHCCCCCCCCCCCHHCCCCCCCCCC
CTFCAGGPETEPGSEEERRKYGANRIAEGKLPMCASLCSTKALLAGDAEKISDIFRQRVV
EEEECCCCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
ERGAKGAGWTNGEDLAYDATRS
HCCCCCCCCCCCCCEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1781728; 14500908 [H]