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The map label for this gene is fdnI [H]

Identifier: 227081669

GI number: 227081669

Start: 1571081

End: 1572154

Strand: Reverse

Name: fdnI [H]

Synonym: VCM66_1453

Alternate gene names: 227081669

Gene position: 1572154-1571081 (Counterclockwise)

Preceding gene: 227081670

Following gene: 227081668

Centisome position: 54.35

GC content: 49.53

Gene sequence:

>1074_bases
GTGGCTATGAGACAACAACTTTCTCGCGCCACTCGTTTTGTGCTGCCTTTATTGGCAGCACTGCTGTGCTGGTTGGCTCA
ACCCGTGATGGCAGAAGAGAACGCAAAACCGGATGTGGTGCAAAAAGAGATGACCCAACTGGCGGGTGCAGACTTTTGGC
GTCAGGTACGACAAGGCGAAGCGGGGTACACCACGTCTCAGTCTCCTGAACATGGCGTGTTGATCAGTAAACCGGGTGAA
ACTTGGTTTATCCTCAAAGAGAAATGGATGTCTCCGGCTGGTGCGGTGGCGATTTTCGGCAGCATAGCAATGGTCGTGCT
AGCGTATATCGCAGTCGGTCCGCTGATGCTGAGTAAGCCGCGCACCGGACGTAAGCTCAATCGCTGGTCACGCTCAGATA
GGGCGCTGCACTGGAGCATGGCGTTTACCTTCCTGACCTTGGCATTCAGTGGCTTGATGCTGGTGTACGGTAAGCACTTC
TTAAAACCGTATATCCCGAGTGAGCTGTGGGGCTGGATCATCTATGCCGCGAAGCAGTATCACAACTACATGGGACCACT
GTTTTTCATTTTGCTGATGTTTGTACTGCTCAAGTGGTGGCGCAAATCCATCTTCAATAAAGTGGATGTGCAGTGGTTTA
TGAAACTCGGTGGCATGGTTGGAAAATACAAAGGCACACATCCTTCAGCAGGCTTTTCCAATGCTGGGGAAAAAGCCATT
TACTGGTTACTGATTGTGTTTGGAACCTTTGCGGCCATTAGTGGTTTGGTGCTCGACTTCCCGATCTTTGGTCAAACACG
TCGTGATATGGAACTGTCGAACCTCATTCATATGCTCTCAGCGTTGATCTTAATCTGTGGCTTTATCTTCCATATTTATA
TCGGTCTGTTTGGTATGGAAGGCGCATTAGAAGGCATGGTGACCGGCAAAGTGGATGAAACGTGGGCGAAAGAGCATCAT
GACCTTTGGTATGAAGAAGTGAAACGCCGCGGTGAAATTGAAGAAGCCAGTACGGAATCTCAACCGGCCTCATTCAATCA
AACGAAAGGAGAGGTGAGCCATGAATCAAGATAG

Upstream 100 bases:

>100_bases
AATCTCCGATATTTTCCGTCAACGCGTAGTAGAACGCGGTGCGAAAGGTGCCGGTTGGACGAACGGTGAAGATCTCGCCT
ATGACGCAACTCGGAGTTAA

Downstream 100 bases:

>100_bases
AGCGCACAAGTCAGTGTGGTTTGTCTACATTGCAAGCTTGCTGACGCCATTCACCTGCTTGCTCTCGGGCGTGATTGGCA
TTATCTATGCGGGTTATCGA

Product: formate dehydrogenase, cytochrome B556 subunit

Products: CO2; NADH

Alternate protein names: Aerobic formate dehydrogenase cytochrome b556 subunit; FDH-Z subunit gamma; Formate dehydrogenase-O subunit gamma [H]

Number of amino acids: Translated: 357; Mature: 356

Protein sequence:

>357_residues
MAMRQQLSRATRFVLPLLAALLCWLAQPVMAEENAKPDVVQKEMTQLAGADFWRQVRQGEAGYTTSQSPEHGVLISKPGE
TWFILKEKWMSPAGAVAIFGSIAMVVLAYIAVGPLMLSKPRTGRKLNRWSRSDRALHWSMAFTFLTLAFSGLMLVYGKHF
LKPYIPSELWGWIIYAAKQYHNYMGPLFFILLMFVLLKWWRKSIFNKVDVQWFMKLGGMVGKYKGTHPSAGFSNAGEKAI
YWLLIVFGTFAAISGLVLDFPIFGQTRRDMELSNLIHMLSALILICGFIFHIYIGLFGMEGALEGMVTGKVDETWAKEHH
DLWYEEVKRRGEIEEASTESQPASFNQTKGEVSHESR

Sequences:

>Translated_357_residues
MAMRQQLSRATRFVLPLLAALLCWLAQPVMAEENAKPDVVQKEMTQLAGADFWRQVRQGEAGYTTSQSPEHGVLISKPGE
TWFILKEKWMSPAGAVAIFGSIAMVVLAYIAVGPLMLSKPRTGRKLNRWSRSDRALHWSMAFTFLTLAFSGLMLVYGKHF
LKPYIPSELWGWIIYAAKQYHNYMGPLFFILLMFVLLKWWRKSIFNKVDVQWFMKLGGMVGKYKGTHPSAGFSNAGEKAI
YWLLIVFGTFAAISGLVLDFPIFGQTRRDMELSNLIHMLSALILICGFIFHIYIGLFGMEGALEGMVTGKVDETWAKEHH
DLWYEEVKRRGEIEEASTESQPASFNQTKGEVSHESR
>Mature_356_residues
AMRQQLSRATRFVLPLLAALLCWLAQPVMAEENAKPDVVQKEMTQLAGADFWRQVRQGEAGYTTSQSPEHGVLISKPGET
WFILKEKWMSPAGAVAIFGSIAMVVLAYIAVGPLMLSKPRTGRKLNRWSRSDRALHWSMAFTFLTLAFSGLMLVYGKHFL
KPYIPSELWGWIIYAAKQYHNYMGPLFFILLMFVLLKWWRKSIFNKVDVQWFMKLGGMVGKYKGTHPSAGFSNAGEKAIY
WLLIVFGTFAAISGLVLDFPIFGQTRRDMELSNLIHMLSALILICGFIFHIYIGLFGMEGALEGMVTGKVDETWAKEHHD
LWYEEVKRRGEIEEASTESQPASFNQTKGEVSHESR

Specific function: Allows to use formate as major electron donor during aerobic respiration. Subunit gamma is probably the cytochrome b556(FDO) component of the formate dehydrogenase [H]

COG id: COG2864

COG function: function code C; Cytochrome b subunit of formate dehydrogenase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the formate dehydrogenase gamma subunit family [H]

Homologues:

Organism=Escherichia coli, GI1790325, Length=211, Percent_Identity=27.4881516587678, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1787750, Length=215, Percent_Identity=27.906976744186, Blast_Score=86, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005797
- InterPro:   IPR016174
- InterPro:   IPR006471 [H]

Pfam domain/function: PF00033 Cytochrom_B_N [H]

EC number: 1.2.1.2

Molecular weight: Translated: 40548; Mature: 40417

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAMRQQLSRATRFVLPLLAALLCWLAQPVMAEENAKPDVVQKEMTQLAGADFWRQVRQGE
CCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
AGYTTSQSPEHGVLISKPGETWFILKEKWMSPAGAVAIFGSIAMVVLAYIAVGPLMLSKP
CCCCCCCCCCCCEEEECCCCEEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RTGRKLNRWSRSDRALHWSMAFTFLTLAFSGLMLVYGKHFLKPYIPSELWGWIIYAAKQY
CCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
HNYMGPLFFILLMFVLLKWWRKSIFNKVDVQWFMKLGGMVGKYKGTHPSAGFSNAGEKAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCCCCHHHH
YWLLIVFGTFAAISGLVLDFPIFGQTRRDMELSNLIHMLSALILICGFIFHIYIGLFGME
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GALEGMVTGKVDETWAKEHHDLWYEEVKRRGEIEEASTESQPASFNQTKGEVSHESR
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AMRQQLSRATRFVLPLLAALLCWLAQPVMAEENAKPDVVQKEMTQLAGADFWRQVRQGE
CHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
AGYTTSQSPEHGVLISKPGETWFILKEKWMSPAGAVAIFGSIAMVVLAYIAVGPLMLSKP
CCCCCCCCCCCCEEEECCCCEEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RTGRKLNRWSRSDRALHWSMAFTFLTLAFSGLMLVYGKHFLKPYIPSELWGWIIYAAKQY
CCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
HNYMGPLFFILLMFVLLKWWRKSIFNKVDVQWFMKLGGMVGKYKGTHPSAGFSNAGEKAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCCCCHHHH
YWLLIVFGTFAAISGLVLDFPIFGQTRRDMELSNLIHMLSALILICGFIFHIYIGLFGME
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GALEGMVTGKVDETWAKEHHDLWYEEVKRRGEIEEASTESQPASFNQTKGEVSHESR
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]