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The map label for this gene is hlyB [H]

Identifier: 227081576

GI number: 227081576

Start: 1453744

End: 1455531

Strand: Direct

Name: hlyB [H]

Synonym: VCM66_1360

Alternate gene names: 227081576

Gene position: 1453744-1455531 (Clockwise)

Preceding gene: 227081564

Following gene: 227081577

Centisome position: 50.26

GC content: 44.8

Gene sequence:

>1788_bases
TTGTCTATTTACTTAAAGGCTTTGATACCGAGTAGGTTGAGACACTTTTGGAGCCATATTTTATGGCATGTGACATGGAA
CGTGAGGCGCACTGTGATGAAATGGTTTAATAATTTATCGATTACCCAAAAAATGATTGCTCTGGTTGGGTGTTTACTTG
CTCTAACGTTAATGGTTTCCACTTATTCTATTACTAAGATGAAACGAGTAGCGGTTGAGATAGAGGCGATAGCTCACGAT
AACTTACCTTTAGCCAAATTGATGACCGACATGACCGTTCATCAACTCGAAGGGGCGATTACTCTTGAACGAGTCTTCCG
CGCAGCAGGCATCGAAAGTAGTCAAGGCAAGCAGCAGCAAAATCAGTATCAAGCACAGCTCATGGAGTTAGTGAATAAAT
TCAAGCAAGAATTTAACCAATCTCGACAACTACTTGAATCTTCTATAGAACATACGATTTCCCCCTCAATACGTAGCTAT
CTGGCTAATCTAGAGCAAGACCTTGACTCATTAGGTCGTGAACATGAAGAATTTGAAAGAAAGCTCAATGAGTTGCTGAA
TGAACTTCGTTCTGGCAGAGAGGTTAAATTATTAGTAGCAGATGCCGAGCAGATTGAACAATTACAAATCAGCCTAGATC
AGCGTTTAATCGATATTCTGCGTAAAATCGAGCAAGCAACTGAGCAATCTGTCTTAGTGACAGAGCAAGAAGAACGTGAG
GCGCTATGGGGAATGATTTATTTAAGCGCCTTTGCGATTTTATTCGGGTTAATTCTCGGGTTTGCGTTTAGTAAACAAAT
CGTTATGGCGATTGCTAGAGCCCGTAAGTTGGCCAATGAAATGGCTGAAGGAAATTTTAGCAAGCGAGCTAAAGTCACTA
CAGGTGACGAAATTGGCCAGCTCATTACCAGTATGAATACCATGGCTCAATCTCTGAGCCACATCGTCGGTGAGGTAATA
GACAGAGCAAATACGATTGCCTCAACCGTGACACAACTTGCTTCTTCGGCAGAAAGCAATAAGAAGTCAGTACAACAGCA
GCAAGCCAATACAGAACAAGTCACCAGCGCAATGGCGCAAATGGCAATCACGATTACGGAAGTGGCGAGCAGTGCGGAAG
AGTCCTCCGCTGCGACAGCAAGGGCACAGGAAAATGCACGCTACAGCTGCGATGTCTTGGGTAAAACTGAGACGGTTTCT
AGTCAATTGGTTGCTAATGCCCAGCAATCACAGCAAATGATTGTTGAGTTAGAGGCCAGTACTCGGCAGATCGAAAGTTT
TGTGTTAGTCGTAGAAGGAATTTCGGAACAAACTAACCTACTGGCTTTAAATGCAGCGATCGAAGCGGCGCGTGCCGGTG
AACAAGGCCGTGGATTTGCTGTGGTTGCGGATGAGGTGCGAGCTCTGGCATCTCGCAGCCAACAGGCAACACATGAAATT
AAGGGCTTGATCCAGACCTTAGTGGAACGTGCGCAATCGGCTACTAAGATGATTGACTCAAGCGATCGTCAGATAGAAGA
AAGCTTTTCATCGATTACCGCCGCAAAAAAACAGCTCGATTCGATTAATCTTGCTCTTCTAGAACTCACGTCCGCCAATA
CCCAAGTAGCGGCAGCCAGTGAGGAACAATCGGTTGCCGCAGATGAGATTAGCCACAACATGACGGATATTCGCGATGCT
GGGGAAACCATCATGCTCAGTGCACAAGAAACGGCTCAAGCGAGTGAAGAGTTGGCACAACAAGCCCAAGGATTAAAATT
ATTGATGGGACGCTTTGTTATTTCGTAA

Upstream 100 bases:

>100_bases
TAGTTTCATAATCTAAAATACTTAAGCAAAGGCTTAAATTTAAAACAAACAAAAAGAAAAATTTGTACTAGAGGTTAGTA
CCTCGCGTCGGCTAGCGGTT

Downstream 100 bases:

>100_bases
CAGTGTGTTGTCACTAAACGAGTAGAGTATTACAAACATAAAAGATAAGGCTCTCTACTATAATGAGTTTGAGAGTCATT
GATTTGGAAGCCATTTATGC

Product: methyl-accepting chemotaxis protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 595; Mature: 594

Protein sequence:

>595_residues
MSIYLKALIPSRLRHFWSHILWHVTWNVRRTVMKWFNNLSITQKMIALVGCLLALTLMVSTYSITKMKRVAVEIEAIAHD
NLPLAKLMTDMTVHQLEGAITLERVFRAAGIESSQGKQQQNQYQAQLMELVNKFKQEFNQSRQLLESSIEHTISPSIRSY
LANLEQDLDSLGREHEEFERKLNELLNELRSGREVKLLVADAEQIEQLQISLDQRLIDILRKIEQATEQSVLVTEQEERE
ALWGMIYLSAFAILFGLILGFAFSKQIVMAIARARKLANEMAEGNFSKRAKVTTGDEIGQLITSMNTMAQSLSHIVGEVI
DRANTIASTVTQLASSAESNKKSVQQQQANTEQVTSAMAQMAITITEVASSAEESSAATARAQENARYSCDVLGKTETVS
SQLVANAQQSQQMIVELEASTRQIESFVLVVEGISEQTNLLALNAAIEAARAGEQGRGFAVVADEVRALASRSQQATHEI
KGLIQTLVERAQSATKMIDSSDRQIEESFSSITAAKKQLDSINLALLELTSANTQVAAASEEQSVAADEISHNMTDIRDA
GETIMLSAQETAQASEELAQQAQGLKLLMGRFVIS

Sequences:

>Translated_595_residues
MSIYLKALIPSRLRHFWSHILWHVTWNVRRTVMKWFNNLSITQKMIALVGCLLALTLMVSTYSITKMKRVAVEIEAIAHD
NLPLAKLMTDMTVHQLEGAITLERVFRAAGIESSQGKQQQNQYQAQLMELVNKFKQEFNQSRQLLESSIEHTISPSIRSY
LANLEQDLDSLGREHEEFERKLNELLNELRSGREVKLLVADAEQIEQLQISLDQRLIDILRKIEQATEQSVLVTEQEERE
ALWGMIYLSAFAILFGLILGFAFSKQIVMAIARARKLANEMAEGNFSKRAKVTTGDEIGQLITSMNTMAQSLSHIVGEVI
DRANTIASTVTQLASSAESNKKSVQQQQANTEQVTSAMAQMAITITEVASSAEESSAATARAQENARYSCDVLGKTETVS
SQLVANAQQSQQMIVELEASTRQIESFVLVVEGISEQTNLLALNAAIEAARAGEQGRGFAVVADEVRALASRSQQATHEI
KGLIQTLVERAQSATKMIDSSDRQIEESFSSITAAKKQLDSINLALLELTSANTQVAAASEEQSVAADEISHNMTDIRDA
GETIMLSAQETAQASEELAQQAQGLKLLMGRFVIS
>Mature_594_residues
SIYLKALIPSRLRHFWSHILWHVTWNVRRTVMKWFNNLSITQKMIALVGCLLALTLMVSTYSITKMKRVAVEIEAIAHDN
LPLAKLMTDMTVHQLEGAITLERVFRAAGIESSQGKQQQNQYQAQLMELVNKFKQEFNQSRQLLESSIEHTISPSIRSYL
ANLEQDLDSLGREHEEFERKLNELLNELRSGREVKLLVADAEQIEQLQISLDQRLIDILRKIEQATEQSVLVTEQEEREA
LWGMIYLSAFAILFGLILGFAFSKQIVMAIARARKLANEMAEGNFSKRAKVTTGDEIGQLITSMNTMAQSLSHIVGEVID
RANTIASTVTQLASSAESNKKSVQQQQANTEQVTSAMAQMAITITEVASSAEESSAATARAQENARYSCDVLGKTETVSS
QLVANAQQSQQMIVELEASTRQIESFVLVVEGISEQTNLLALNAAIEAARAGEQGRGFAVVADEVRALASRSQQATHEIK
GLIQTLVERAQSATKMIDSSDRQIEESFSSITAAKKQLDSINLALLELTSANTQVAAASEEQSVAADEISHNMTDIRDAG
ETIMLSAQETAQASEELAQQAQGLKLLMGRFVIS

Specific function: May form a pore through which the hemolysin can be exported [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 methyl-accepting transducer domain [H]

Homologues:

Organism=Escherichia coli, GI2367378, Length=381, Percent_Identity=28.6089238845144, Blast_Score=138, Evalue=1e-33,
Organism=Escherichia coli, GI1788195, Length=292, Percent_Identity=30.8219178082192, Blast_Score=127, Evalue=3e-30,
Organism=Escherichia coli, GI1787690, Length=399, Percent_Identity=29.8245614035088, Blast_Score=126, Evalue=3e-30,
Organism=Escherichia coli, GI1788194, Length=330, Percent_Identity=31.2121212121212, Blast_Score=126, Evalue=3e-30,
Organism=Escherichia coli, GI1789453, Length=294, Percent_Identity=27.5510204081633, Blast_Score=100, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004090
- InterPro:   IPR004089
- InterPro:   IPR003660
- InterPro:   IPR000727 [H]

Pfam domain/function: PF00672 HAMP; PF00015 MCPsignal [H]

EC number: NA

Molecular weight: Translated: 65776; Mature: 65645

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS50885 HAMP ; PS50111 CHEMOTAXIS_TRANSDUC_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIYLKALIPSRLRHFWSHILWHVTWNVRRTVMKWFNNLSITQKMIALVGCLLALTLMVS
CCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
TYSITKMKRVAVEIEAIAHDNLPLAKLMTDMTVHQLEGAITLERVFRAAGIESSQGKQQQ
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
NQYQAQLMELVNKFKQEFNQSRQLLESSIEHTISPSIRSYLANLEQDLDSLGREHEEFER
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHH
KLNELLNELRSGREVKLLVADAEQIEQLQISLDQRLIDILRKIEQATEQSVLVTEQEERE
HHHHHHHHHHCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCHHHHH
ALWGMIYLSAFAILFGLILGFAFSKQIVMAIARARKLANEMAEGNFSKRAKVTTGDEIGQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
LITSMNTMAQSLSHIVGEVIDRANTIASTVTQLASSAESNKKSVQQQQANTEQVTSAMAQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
MAITITEVASSAEESSAATARAQENARYSCDVLGKTETVSSQLVANAQQSQQMIVELEAS
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
TRQIESFVLVVEGISEQTNLLALNAAIEAARAGEQGRGFAVVADEVRALASRSQQATHEI
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHH
KGLIQTLVERAQSATKMIDSSDRQIEESFSSITAAKKQLDSINLALLELTSANTQVAAAS
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCEEECC
EEQSVAADEISHNMTDIRDAGETIMLSAQETAQASEELAQQAQGLKLLMGRFVIS
CHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SIYLKALIPSRLRHFWSHILWHVTWNVRRTVMKWFNNLSITQKMIALVGCLLALTLMVS
CEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
TYSITKMKRVAVEIEAIAHDNLPLAKLMTDMTVHQLEGAITLERVFRAAGIESSQGKQQQ
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
NQYQAQLMELVNKFKQEFNQSRQLLESSIEHTISPSIRSYLANLEQDLDSLGREHEEFER
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHH
KLNELLNELRSGREVKLLVADAEQIEQLQISLDQRLIDILRKIEQATEQSVLVTEQEERE
HHHHHHHHHHCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCHHHHH
ALWGMIYLSAFAILFGLILGFAFSKQIVMAIARARKLANEMAEGNFSKRAKVTTGDEIGQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
LITSMNTMAQSLSHIVGEVIDRANTIASTVTQLASSAESNKKSVQQQQANTEQVTSAMAQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
MAITITEVASSAEESSAATARAQENARYSCDVLGKTETVSSQLVANAQQSQQMIVELEAS
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
TRQIESFVLVVEGISEQTNLLALNAAIEAARAGEQGRGFAVVADEVRALASRSQQATHEI
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHH
KGLIQTLVERAQSATKMIDSSDRQIEESFSSITAAKKQLDSINLALLELTSANTQVAAAS
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCEEECC
EEQSVAADEISHNMTDIRDAGETIMLSAQETAQASEELAQQAQGLKLLMGRFVIS
CHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2162464; 10952301 [H]