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The map label for this gene is rlmI [H]

Identifier: 227081525

GI number: 227081525

Start: 1402251

End: 1403507

Strand: Reverse

Name: rlmI [H]

Synonym: VCM66_1309

Alternate gene names: 227081525

Gene position: 1403507-1402251 (Counterclockwise)

Preceding gene: 227081527

Following gene: 227081522

Centisome position: 48.52

GC content: 46.3

Gene sequence:

>1257_bases
TTGCTTTTCTTCATCAGTTCCACGACAATAGCCGCCCATTTCTATTTCCATCTGAGTAGCGTAATGACTCCTGCAATTTA
TTTGGTCAAAGGTCGAGACAAATCTTTGCGTCGTAAACATCCGTGGGTTTTTTCTCGTGGGATCAGCAAAGTCGAAGGGA
CTCCTAAACTGGGAGAAACTGTCGATGTTTACTCCTTTGAAGGCAAATGGTTAGCCAAAGCGGCCTACTCACCGCATTCA
CAAATTACTGCGCGTGTCTGGAGCTTTGAACAAGAGCCGATTGATCGCGATTTCTTCATTAAACGCATCGAACAAGCACA
ATTATTGCGCAACGATATTATCGAACGTGATGGCTTAACCGGTTACCGTTTAATTGCCGCAGAATCAGACGGCTTACCTG
GCATTACTATCGACAAATATCAAGATTATTTGGTCTGCCAACTACTGAGTGCCGGTGCCGAGTATCAAAAACAGACTCTG
GTCGAGGCATTATTACATTGCTTCCCTGAGTGCCATATTTATGAGCGTTCCGATGTTGCGGTGCGTAAAAAAGAAGGGCT
GGACGAGCGCGTTGGGGTACTGCACGGTGAATTACCACCTAAATCGGTCGTGATTGAAGAAAATGGCGTTAAGATCAGCG
TCGATATTGTGGGTGGACACAAAACGGGTTTCTATCTAGATCAACGCGACAGCCGCTTCCAATCCATGAAATACGTTAAA
GAGAAAGAAGTGCTGAACTGCTTCTCTTACACGGGTGGTTTTGGTTTATACGCACTCAAAGGCGGAGCTAAGCGTGTGAT
CAACGCAGATGTCTCTCAACCAGCCTTGGATACGGCTAAGTTCAATGCTGAATTGAACGGTTTCGATATCAGTAAAAAGC
GTGCGGTGTTCTTGAATGCCGATGTATTTAAACTGCTGCGCGAATACCGCGACCAAGGCACACGTTTTGATGTGGTGGTG
ATGGATCCCCCCAAGTTTGCCGAATCTAAAGCCCAGCTGGATGGTGCATGCCGCGGCTATAAAGACATCAACATGCTGGC
CATGCAGATCTTAAACCCAGGTGGCACCCTGCTCACCTACTCGTGTTCAGGGTTAATGGATCAAGTGCTATTTCAGAAAA
TTATTGCCGATGCGGCTCTCGATGCTGGACGTGATGTAAAGTTTGTTGAGCGTTTTGAACAAGCGGCCGATCACCCAACC
GATACCGCTTATCCTGAAGGTTTTTATCTCAAAGGCTTTGCTTGTAAGGTTTTATAA

Upstream 100 bases:

>100_bases
CGACTTTAATGAAACTGGTTCAAGCATAGCCGAAATCCACGTTATCCCAAAAAGAGTTGATAACCCCTTACCTTCGAATC
ATAAGGAAGGGGCTTTTCTT

Downstream 100 bases:

>100_bases
GCGATGGCGTGAGCGAACGTTAGCAAGCATAGAAAGCGGTCTCAATGGAGGCCGCTTTTTACTTGCCACTCTTCACTTTT
CACCACGCGGTTACGCCCGA

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 418; Mature: 418

Protein sequence:

>418_residues
MLFFISSTTIAAHFYFHLSSVMTPAIYLVKGRDKSLRRKHPWVFSRGISKVEGTPKLGETVDVYSFEGKWLAKAAYSPHS
QITARVWSFEQEPIDRDFFIKRIEQAQLLRNDIIERDGLTGYRLIAAESDGLPGITIDKYQDYLVCQLLSAGAEYQKQTL
VEALLHCFPECHIYERSDVAVRKKEGLDERVGVLHGELPPKSVVIEENGVKISVDIVGGHKTGFYLDQRDSRFQSMKYVK
EKEVLNCFSYTGGFGLYALKGGAKRVINADVSQPALDTAKFNAELNGFDISKKRAVFLNADVFKLLREYRDQGTRFDVVV
MDPPKFAESKAQLDGACRGYKDINMLAMQILNPGGTLLTYSCSGLMDQVLFQKIIADAALDAGRDVKFVERFEQAADHPT
DTAYPEGFYLKGFACKVL

Sequences:

>Translated_418_residues
MLFFISSTTIAAHFYFHLSSVMTPAIYLVKGRDKSLRRKHPWVFSRGISKVEGTPKLGETVDVYSFEGKWLAKAAYSPHS
QITARVWSFEQEPIDRDFFIKRIEQAQLLRNDIIERDGLTGYRLIAAESDGLPGITIDKYQDYLVCQLLSAGAEYQKQTL
VEALLHCFPECHIYERSDVAVRKKEGLDERVGVLHGELPPKSVVIEENGVKISVDIVGGHKTGFYLDQRDSRFQSMKYVK
EKEVLNCFSYTGGFGLYALKGGAKRVINADVSQPALDTAKFNAELNGFDISKKRAVFLNADVFKLLREYRDQGTRFDVVV
MDPPKFAESKAQLDGACRGYKDINMLAMQILNPGGTLLTYSCSGLMDQVLFQKIIADAALDAGRDVKFVERFEQAADHPT
DTAYPEGFYLKGFACKVL
>Mature_418_residues
MLFFISSTTIAAHFYFHLSSVMTPAIYLVKGRDKSLRRKHPWVFSRGISKVEGTPKLGETVDVYSFEGKWLAKAAYSPHS
QITARVWSFEQEPIDRDFFIKRIEQAQLLRNDIIERDGLTGYRLIAAESDGLPGITIDKYQDYLVCQLLSAGAEYQKQTL
VEALLHCFPECHIYERSDVAVRKKEGLDERVGVLHGELPPKSVVIEENGVKISVDIVGGHKTGFYLDQRDSRFQSMKYVK
EKEVLNCFSYTGGFGLYALKGGAKRVINADVSQPALDTAKFNAELNGFDISKKRAVFLNADVFKLLREYRDQGTRFDVVV
MDPPKFAESKAQLDGACRGYKDINMLAMQILNPGGTLLTYSCSGLMDQVLFQKIIADAALDAGRDVKFVERFEQAADHPT
DTAYPEGFYLKGFACKVL

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=398, Percent_Identity=62.8140703517588, Blast_Score=525, Evalue=1e-150,
Organism=Escherichia coli, GI1787181, Length=255, Percent_Identity=27.843137254902, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 47001; Mature: 47001

Theoretical pI: Translated: 7.52; Mature: 7.52

Prosite motif: PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFFISSTTIAAHFYFHLSSVMTPAIYLVKGRDKSLRRKHPWVFSRGISKVEGTPKLGET
CEEEEECHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHCCCCHHHCCCHHCCCCCCCCCE
VDVYSFEGKWLAKAAYSPHSQITARVWSFEQEPIDRDFFIKRIEQAQLLRNDIIERDGLT
EEEEEECCCEEEEECCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
GYRLIAAESDGLPGITIDKYQDYLVCQLLSAGAEYQKQTLVEALLHCFPECHIYERSDVA
CEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEECCCCE
VRKKEGLDERVGVLHGELPPKSVVIEENGVKISVDIVGGHKTGFYLDQRDSRFQSMKYVK
EHHHCCCHHHHHHHCCCCCCCEEEEECCCEEEEEEEECCCCCCEEECCCHHHHHHHHHHH
EKEVLNCFSYTGGFGLYALKGGAKRVINADVSQPALDTAKFNAELNGFDISKKRAVFLNA
HHHHHHHHHHCCCCEEEEECCCCCEEEECCCCCCCCCHHHCCCCCCCCCCCCCEEEEECH
DVFKLLREYRDQGTRFDVVVMDPPKFAESKAQLDGACRGYKDINMLAMQILNPGGTLLTY
HHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEEE
SCSGLMDQVLFQKIIADAALDAGRDVKFVERFEQAADHPTDTAYPEGFYLKGFACKVL
EHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCEEECCEEEECC
>Mature Secondary Structure
MLFFISSTTIAAHFYFHLSSVMTPAIYLVKGRDKSLRRKHPWVFSRGISKVEGTPKLGET
CEEEEECHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHCCCCHHHCCCHHCCCCCCCCCE
VDVYSFEGKWLAKAAYSPHSQITARVWSFEQEPIDRDFFIKRIEQAQLLRNDIIERDGLT
EEEEEECCCEEEEECCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
GYRLIAAESDGLPGITIDKYQDYLVCQLLSAGAEYQKQTLVEALLHCFPECHIYERSDVA
CEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEECCCCE
VRKKEGLDERVGVLHGELPPKSVVIEENGVKISVDIVGGHKTGFYLDQRDSRFQSMKYVK
EHHHCCCHHHHHHHCCCCCCCEEEEECCCEEEEEEEECCCCCCEEECCCHHHHHHHHHHH
EKEVLNCFSYTGGFGLYALKGGAKRVINADVSQPALDTAKFNAELNGFDISKKRAVFLNA
HHHHHHHHHHCCCCEEEEECCCCCEEEECCCCCCCCCHHHCCCCCCCCCCCCCEEEEECH
DVFKLLREYRDQGTRFDVVVMDPPKFAESKAQLDGACRGYKDINMLAMQILNPGGTLLTY
HHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEEE
SCSGLMDQVLFQKIIADAALDAGRDVKFVERFEQAADHPTDTAYPEGFYLKGFACKVL
EHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCEEECCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA