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The map label for this gene is maiA [H]

Identifier: 227081518

GI number: 227081518

Start: 1392164

End: 1392811

Strand: Direct

Name: maiA [H]

Synonym: VCM66_1302

Alternate gene names: 227081518

Gene position: 1392164-1392811 (Clockwise)

Preceding gene: 227081517

Following gene: 227081520

Centisome position: 48.13

GC content: 48.15

Gene sequence:

>648_bases
GTGATGAGTTTAATTCTCTATGGTTATTGGCGTTCGTCAGCCGCCTATCGAGTGCGTATTGCCCTCAATATTAAACAGTT
GGTTTACGAGTCCCGTGCCGTGCATCTTAGCCGTGAAGGAGGGGAGCAGCATCATGCCGAGTTTCATCGCCTCAATCCTA
GCGAGTTGATTCCTGTGCTTATCGATGGTGAGCTCTGCTTGAATCAATCGCTGGCGATTATTGAGTATTTGGATGAAACC
TATCCCGAACCCCGTTTGATCCCAGAGCGAGGTGCCGAGCGTTATCAGGTGAAAGCGTTAGCGTTAGACATCGCAGCGGA
TATCCATCCGATCAATAACTTGCGGATTTTGCAGTATCTTACCGCGGAGCTTGGTGTGGCCGATGAAGAAAAAAATCGTT
GGTATCGCCACTGGATTGATAAAGGTTTTCAAGGTTTGGAAGAGAAATTGCGCCATACGGCAGGGGAATATTGTGTGGGT
AATCGCTTGTCTCTGGTCGATGTCTGTTTAGTGCCTCAAGTTTATAACGCTGAGCGATTTGATCTCGATATGAGTCGTTA
TCCCACCTTACAGCAGATTGCAGCAAGGTTACGTGCTTTGCCTGCGTTTGCGCAGGCTGCTCCAGAAAATCAGCCTGATG
CTTGTTGA

Upstream 100 bases:

>100_bases
TGCGCTTTGGTGATACGGTGCGAATTGAGATGCTCAATCCACAGCAACAGAGTATTTTTGGTGCGATTGAGCAACAGGTG
ATGCAGTACAACAAGGAAGT

Downstream 100 bases:

>100_bases
CCCCGTTGAATCGGTACCATAGCAGATTCTTCATCGGCAAACTTTTGTGCAATGTCGATGAAGTTCTGCTCCTCCAGCAA
AAAAGCATCAATCACCATTG

Product: maleylacetoacetate isomerase

Products: NA

Alternate protein names: MAAI [H]

Number of amino acids: Translated: 215; Mature: 215

Protein sequence:

>215_residues
MMSLILYGYWRSSAAYRVRIALNIKQLVYESRAVHLSREGGEQHHAEFHRLNPSELIPVLIDGELCLNQSLAIIEYLDET
YPEPRLIPERGAERYQVKALALDIAADIHPINNLRILQYLTAELGVADEEKNRWYRHWIDKGFQGLEEKLRHTAGEYCVG
NRLSLVDVCLVPQVYNAERFDLDMSRYPTLQQIAARLRALPAFAQAAPENQPDAC

Sequences:

>Translated_215_residues
MMSLILYGYWRSSAAYRVRIALNIKQLVYESRAVHLSREGGEQHHAEFHRLNPSELIPVLIDGELCLNQSLAIIEYLDET
YPEPRLIPERGAERYQVKALALDIAADIHPINNLRILQYLTAELGVADEEKNRWYRHWIDKGFQGLEEKLRHTAGEYCVG
NRLSLVDVCLVPQVYNAERFDLDMSRYPTLQQIAARLRALPAFAQAAPENQPDAC
>Mature_215_residues
MMSLILYGYWRSSAAYRVRIALNIKQLVYESRAVHLSREGGEQHHAEFHRLNPSELIPVLIDGELCLNQSLAIIEYLDET
YPEPRLIPERGAERYQVKALALDIAADIHPINNLRILQYLTAELGVADEEKNRWYRHWIDKGFQGLEEKLRHTAGEYCVG
NRLSLVDVCLVPQVYNAERFDLDMSRYPTLQQIAARLRALPAFAQAAPENQPDAC

Specific function: Forms An Equimolar Complex With The RNA Polymerase Holoenzyme (Rnap) But Not With The Core Enzyme. It Is Synthesized Predominantly When Cells Are Exposed To Amino Acid Starvation, At Which Time It Accounts For Over 50% Of The Total Protein Synthesized. It

COG id: COG0625

COG function: function code O; Glutathione S-transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI22202624, Length=211, Percent_Identity=45.0236966824645, Blast_Score=184, Evalue=3e-47,
Organism=Homo sapiens, GI22202622, Length=159, Percent_Identity=44.6540880503145, Blast_Score=134, Evalue=6e-32,
Organism=Homo sapiens, GI194440732, Length=211, Percent_Identity=36.0189573459716, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17510461, Length=215, Percent_Identity=43.7209302325581, Blast_Score=174, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI17556142, Length=212, Percent_Identity=43.8679245283019, Blast_Score=165, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17551302, Length=211, Percent_Identity=37.4407582938389, Blast_Score=157, Evalue=4e-39,
Organism=Drosophila melanogaster, GI21355859, Length=211, Percent_Identity=44.0758293838863, Blast_Score=189, Evalue=8e-49,
Organism=Drosophila melanogaster, GI24645375, Length=211, Percent_Identity=44.0758293838863, Blast_Score=189, Evalue=9e-49,
Organism=Drosophila melanogaster, GI45553325, Length=215, Percent_Identity=43.7209302325581, Blast_Score=189, Evalue=1e-48,
Organism=Drosophila melanogaster, GI21355857, Length=212, Percent_Identity=39.622641509434, Blast_Score=171, Evalue=2e-43,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1982 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR005955
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: =5.2.1.2 [H]

Molecular weight: Translated: 24688; Mature: 24688

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSLILYGYWRSSAAYRVRIALNIKQLVYESRAVHLSREGGEQHHAEFHRLNPSELIPVL
CCEEEEEEEECCCCEEEEEEEECHHHHHHHHHHHEEECCCCHHHHHHHHCCCHHHEEEEE
IDGELCLNQSLAIIEYLDETYPEPRLIPERGAERYQVKALALDIAADIHPINNLRILQYL
ECCHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHH
TAELGVADEEKNRWYRHWIDKGFQGLEEKLRHTAGEYCVGNRLSLVDVCLVPQVYNAERF
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCHHC
DLDMSRYPTLQQIAARLRALPAFAQAAPENQPDAC
CCCHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MMSLILYGYWRSSAAYRVRIALNIKQLVYESRAVHLSREGGEQHHAEFHRLNPSELIPVL
CCEEEEEEEECCCCEEEEEEEECHHHHHHHHHHHEEECCCCHHHHHHHHCCCHHHEEEEE
IDGELCLNQSLAIIEYLDETYPEPRLIPERGAERYQVKALALDIAADIHPINNLRILQYL
ECCHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHH
TAELGVADEEKNRWYRHWIDKGFQGLEEKLRHTAGEYCVGNRLSLVDVCLVPQVYNAERF
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCHHC
DLDMSRYPTLQQIAARLRALPAFAQAAPENQPDAC
CCCHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]