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The map label for this gene is sdaA-1 [H]

Identifier: 227081471

GI number: 227081471

Start: 1337466

End: 1338827

Strand: Reverse

Name: sdaA-1 [H]

Synonym: VCM66_1255

Alternate gene names: 227081471

Gene position: 1338827-1337466 (Counterclockwise)

Preceding gene: 227081472

Following gene: 227081469

Centisome position: 46.29

GC content: 49.63

Gene sequence:

>1362_bases
ATGATTAGTGTATTTGATATCTATAAAATCGGTGTTGGTCCTTCAAGTTCACACACGGTTGGACCGATGAAAGCGGGTAA
AGAGTTTATTGACGAACTACGCTCAATGGGAAAATTGCGCGACGTCACTAAAATCACCGTGGACGTTTATGGATCGCTAT
CACTGACAGGGAAAGGTCACCACACGGATATCGCTATCATCATGGGTCTGGCAGGCAATACGCCAGAGAAAGTGGATATC
GACTCGATTCCGAGCTTTATCGCTCGTGTAGAAGAAACAGAACGTCTTCCTGTTGGTATGCACTGTCATACCGTCTCTTT
CCCTCGTGAAGGTGGGATGAATTTCCACCGCACTAATCTGCCTTTGCACGAAAACGGCATGCAGATTCACGCATGGATCA
ACGACGAGAAAATCTTCAGCAAAACTTACTACTCTATCGGTGGTGGTTTCATTGTTGATGAAGAGCACTTTGGTAAAGAG
AACGAATCTCTTATTCAAGTGCCTTACGCTTTCCGTTCAGCGGAAGAGCTGCTGAACCAATGTAAAGAGAGTGGCCTCTC
GATCAGCACGCTCGTTATGGCGAACGAAAAAGCCATGCATTCTGACGAAGAAGTGCGCACCTATTTCGCGAACATTTGGC
GTACCATGCGTGAGTGTATGGAACGCGGGATGAACACCGAAGGCATCCTACCTGGGCCTTTGCGTGTACCACGCCGTGCG
GCAGCGCTGCGTCAACAACTGCTGACGTCCGAAAAAACCACCAACGATCCAATGGCGGTGGTTGACTGGGTAAACATGTA
TGCGTTTGCGGTTAACGAAGAAAACGCAGCCGGTGGCCGTGTAGTCACTGCACCGACTAACGGTGCGTGTGGCATTATCC
CTGCGGTACTCGCCTACTACGACAAATTCATCCAAACCGTGACTGAGAAAGACTACATCCGTTACTTCGCTGCTTCTGGC
GCGATTGGTGGTTTGTACAAACGTAATGCGTCTATCTCAGGTGCAGAAGTGGGCTGTCAAGGTGAAGTCGGTGTCGCTTG
TTCTATGGCAGCAGCGGGTCTTGCTGAGCTTTTAGGCGGTAGCCCAGAGCAAGTCTGTATGGCGGCTGAAATCGCGATGG
AGCATAACCTTGGTTTGACTTGTGACCCTGTTGCAGGACAAGTACAGGTGCCATGTATCGAACGTAACGGCATTGCAGCG
GTGAAAGCGATCAACTCAACCCGTATGGCGCTGCGTCGTTCTTCTGCCCCACGTGTTTCACTGGATAAAGTGATTGAAAC
CATGCTGGAAACCGGCAAGGACATGAATGCTAAGTACCGTGAAACCTCACAAGGTGGCTTGGCGATCAAAGTGATCTGTT
AA

Upstream 100 bases:

>100_bases
CTCTTTAATCTCTACCTCTGCGTATGAGCTTAAAACACAAAACATAACTATACCAAAAGCCTCCCTACCCTTAGGGGGGC
TTAGTTTTGAGGCATTCAAA

Downstream 100 bases:

>100_bases
TCAGAACCTTTTTGTGCTTACAAAAACGCCCGGATTTCCGGGCGTTTTTTATGTGGGGCGTTTTCACTCTGAATCTCAAA
TCGATTCGCTATTCACCACG

Product: L-serine dehydratase

Products: NA

Alternate protein names: SDH 1; L-serine deaminase 1; L-SD1 [H]

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MISVFDIYKIGVGPSSSHTVGPMKAGKEFIDELRSMGKLRDVTKITVDVYGSLSLTGKGHHTDIAIIMGLAGNTPEKVDI
DSIPSFIARVEETERLPVGMHCHTVSFPREGGMNFHRTNLPLHENGMQIHAWINDEKIFSKTYYSIGGGFIVDEEHFGKE
NESLIQVPYAFRSAEELLNQCKESGLSISTLVMANEKAMHSDEEVRTYFANIWRTMRECMERGMNTEGILPGPLRVPRRA
AALRQQLLTSEKTTNDPMAVVDWVNMYAFAVNEENAAGGRVVTAPTNGACGIIPAVLAYYDKFIQTVTEKDYIRYFAASG
AIGGLYKRNASISGAEVGCQGEVGVACSMAAAGLAELLGGSPEQVCMAAEIAMEHNLGLTCDPVAGQVQVPCIERNGIAA
VKAINSTRMALRRSSAPRVSLDKVIETMLETGKDMNAKYRETSQGGLAIKVIC

Sequences:

>Translated_453_residues
MISVFDIYKIGVGPSSSHTVGPMKAGKEFIDELRSMGKLRDVTKITVDVYGSLSLTGKGHHTDIAIIMGLAGNTPEKVDI
DSIPSFIARVEETERLPVGMHCHTVSFPREGGMNFHRTNLPLHENGMQIHAWINDEKIFSKTYYSIGGGFIVDEEHFGKE
NESLIQVPYAFRSAEELLNQCKESGLSISTLVMANEKAMHSDEEVRTYFANIWRTMRECMERGMNTEGILPGPLRVPRRA
AALRQQLLTSEKTTNDPMAVVDWVNMYAFAVNEENAAGGRVVTAPTNGACGIIPAVLAYYDKFIQTVTEKDYIRYFAASG
AIGGLYKRNASISGAEVGCQGEVGVACSMAAAGLAELLGGSPEQVCMAAEIAMEHNLGLTCDPVAGQVQVPCIERNGIAA
VKAINSTRMALRRSSAPRVSLDKVIETMLETGKDMNAKYRETSQGGLAIKVIC
>Mature_453_residues
MISVFDIYKIGVGPSSSHTVGPMKAGKEFIDELRSMGKLRDVTKITVDVYGSLSLTGKGHHTDIAIIMGLAGNTPEKVDI
DSIPSFIARVEETERLPVGMHCHTVSFPREGGMNFHRTNLPLHENGMQIHAWINDEKIFSKTYYSIGGGFIVDEEHFGKE
NESLIQVPYAFRSAEELLNQCKESGLSISTLVMANEKAMHSDEEVRTYFANIWRTMRECMERGMNTEGILPGPLRVPRRA
AALRQQLLTSEKTTNDPMAVVDWVNMYAFAVNEENAAGGRVVTAPTNGACGIIPAVLAYYDKFIQTVTEKDYIRYFAASG
AIGGLYKRNASISGAEVGCQGEVGVACSMAAAGLAELLGGSPEQVCMAAEIAMEHNLGLTCDPVAGQVQVPCIERNGIAA
VKAINSTRMALRRSSAPRVSLDKVIETMLETGKDMNAKYRETSQGGLAIKVIC

Specific function: Deaminates also threonine, particularly when it is present in high concentration [H]

COG id: COG1760

COG function: function code E; L-serine deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-sulfur dependent L-serine dehydratase family [H]

Homologues:

Organism=Escherichia coli, GI1788116, Length=453, Percent_Identity=75.0551876379691, Blast_Score=724, Evalue=0.0,
Organism=Escherichia coli, GI1789161, Length=452, Percent_Identity=71.6814159292035, Blast_Score=683, Evalue=0.0,
Organism=Escherichia coli, GI48994925, Length=453, Percent_Identity=70.4194260485651, Blast_Score=662, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004644
- InterPro:   IPR005130
- InterPro:   IPR005131 [H]

Pfam domain/function: PF03313 SDH_alpha; PF03315 SDH_beta [H]

EC number: =4.3.1.17 [H]

Molecular weight: Translated: 49253; Mature: 49253

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISVFDIYKIGVGPSSSHTVGPMKAGKEFIDELRSMGKLRDVTKITVDVYGSLSLTGKGH
CCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEEEECCCCC
HTDIAIIMGLAGNTPEKVDIDSIPSFIARVEETERLPVGMHCHTVSFPREGGMNFHRTNL
CCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCEEECCC
PLHENGMQIHAWINDEKIFSKTYYSIGGGFIVDEEHFGKENESLIQVPYAFRSAEELLNQ
CCCCCCCEEEEEECCCHHHHHHHHHCCCCEEEEHHHCCCCCCCEEECCHHHHHHHHHHHH
CKESGLSISTLVMANEKAMHSDEEVRTYFANIWRTMRECMERGMNTEGILPGPLRVPRRA
HHHCCCCEEEEEEECCHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHH
AALRQQLLTSEKTTNDPMAVVDWVNMYAFAVNEENAAGGRVVTAPTNGACGIIPAVLAYY
HHHHHHHHHHCCCCCCCHHHHHHHHHEEEEECCCCCCCCEEEECCCCCCCHHHHHHHHHH
DKFIQTVTEKDYIRYFAASGAIGGLYKRNASISGAEVGCQGEVGVACSMAAAGLAELLGG
HHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCC
SPEQVCMAAEIAMEHNLGLTCDPVAGQVQVPCIERNGIAAVKAINSTRMALRRSSAPRVS
CHHHHHHHHHHHHHCCCCCEECCCCCCEEEEEECCCCCEEEEEHHHHHHHHHHCCCCCCC
LDKVIETMLETGKDMNAKYRETSQGGLAIKVIC
HHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEC
>Mature Secondary Structure
MISVFDIYKIGVGPSSSHTVGPMKAGKEFIDELRSMGKLRDVTKITVDVYGSLSLTGKGH
CCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEEEECCCCC
HTDIAIIMGLAGNTPEKVDIDSIPSFIARVEETERLPVGMHCHTVSFPREGGMNFHRTNL
CCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCEEECCC
PLHENGMQIHAWINDEKIFSKTYYSIGGGFIVDEEHFGKENESLIQVPYAFRSAEELLNQ
CCCCCCCEEEEEECCCHHHHHHHHHCCCCEEEEHHHCCCCCCCEEECCHHHHHHHHHHHH
CKESGLSISTLVMANEKAMHSDEEVRTYFANIWRTMRECMERGMNTEGILPGPLRVPRRA
HHHCCCCEEEEEEECCHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHH
AALRQQLLTSEKTTNDPMAVVDWVNMYAFAVNEENAAGGRVVTAPTNGACGIIPAVLAYY
HHHHHHHHHHCCCCCCCHHHHHHHHHEEEEECCCCCCCCEEEECCCCCCCHHHHHHHHHH
DKFIQTVTEKDYIRYFAASGAIGGLYKRNASISGAEVGCQGEVGVACSMAAAGLAELLGG
HHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCC
SPEQVCMAAEIAMEHNLGLTCDPVAGQVQVPCIERNGIAAVKAINSTRMALRRSSAPRVS
CHHHHHHHHHHHHHCCCCCEECCCCCCEEEEEECCCCCEEEEEHHHHHHHHHHCCCCCCC
LDKVIETMLETGKDMNAKYRETSQGGLAIKVIC
HHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2504697; 8436113; 9097040; 9278503 [H]