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The map label for this gene is celB [H]

Identifier: 227081454

GI number: 227081454

Start: 1314748

End: 1316088

Strand: Direct

Name: celB [H]

Synonym: VCM66_1237

Alternate gene names: 227081454

Gene position: 1314748-1316088 (Clockwise)

Preceding gene: 227081453

Following gene: 227081455

Centisome position: 45.45

GC content: 45.71

Gene sequence:

>1341_bases
GTGAATCCTATGAAGCTTTATGATGCGATAATTGGAATCGTAGAAAAGCATATAGCCCCCATTGCAGCAAAAGTGGGGAA
TCAGCCTCATGTACGTGCCATGCGTGATGGTTTTATTGTGGCAATGCCATTTATTATTGTGGGTAGTTTTATTTTAATTT
TTGCATTCCCTCCTTTTGCTGAAGATACCACCAATGTTTTTGGTCGCGTGTGGTTAAACTTCGCTACGACGCATTTCGAT
ATCATCATGATGCCGTTTAATATGTCGATGGGCATTATGACCATATTTGTCTCGTTGGGTGTAGCATACAGCTTAGCGAA
AGCTTACAAAATGGACGGTATCACGAGTGCAGTACTGTCACTGATGAGCTTTTTGTTGGTCGCGGCACCCGCCAAAGAAG
ACAGTCTATCGATGGCGCATATGGGCGGCACAGGGATTTTCACGGCTGTTATGTGTGCTTTTTTTGCCGTCGAACTGTAT
CGCTTTATGAAAAAGCATAACATTACCATTCGTATGCCAGAGCAAGTTCCACCTGCGATTGCTCGCTCATTCGAAGTTTT
GTTGCCTGTCTTGGCGATTTTCTTGACGCTTTATCCACTGAGCCTTTTTGTACAAGCCGAATATGGCATGTTGATCCCTG
ATGCCGTGATGGCGATGTTCAAACCGTTGATCAGTGCGTCAAACACATTGCCAGCGATTATCGGTGCGCTGCTGGTGTGT
CAATTGTTATGGTTTGCGGGCATTCATGGGGCTGCGATTGTGGTCGGTCTGCTATCTCCCATCTTCTTAACCAATATCAG
TGCTAACATTGATGCGTTTGTTACTGGGCAGCCCGTCCCGAATATCTTCACACAGCCATTCTGGGATTTTTACATCTTTA
TCGGTGGTTCAGGTGCAACCTTAGCGTTAGTGATTTTGATGTCCTTCAGCCGCTCAGCTCATTTAAAAAGCATCGGTCGT
ATGAGTGCGGTACCAGGATTCTTCCAAATTAACGAACCTGTGATTTTTGGTAGTCCAGTGGTGATGAACCCGATTTTATT
CCTGCCTTTCGTTTTCGCTCCCGTAATCAATGCCACCATTGCTTATTTTGCGATTCAGTTGGGCTTTGTCGGTATGGGAG
TAGCAACCACGCCATGGACAACACCGGCCATTATCGGTGCGTCATGGGGTAGTGGTTGGACGTTTACGCCTGTGTTGCTG
GTCGTGGGTTTACTCATCCTTGATCTGCTCATTTACTTACCTTTCTTCAAAATGTTTGAGAAGCAAGTGCTTGAACAAGA
GCAACCTGTGGAAAGTAAAGTCAAAGCAGCGCAACCAGGTAGACAAGGCGTCACGGCATAA

Upstream 100 bases:

>100_bases
AGCTTTAGATTTAATTAACTAAATCTGTTTTTCGGACAATATTTTCACTCAATCCTCATTAATTAAATGAGGATAGGGAA
CTCTTTATCTTAAATATAAG

Downstream 100 bases:

>100_bases
GCAGTTTAAGGCTGCCGGTGGGCAGCCTTAGCTTCGGGAGAGAGAAATGGAACAAGAACTTATCGTGATGGAAATTATTT
GCAACGCCGGTGAAGCGCGA

Product: PTS system, cellobiose-specific IIC component

Products: pyruvate; diacetylchitobiose-6-phosphate [Cytoplasm]; cellobiose-6-phosphate [Cytoplasm] [C]

Alternate protein names: EIIC-Lic; PTS system lichenan-specific EIIC component [H]

Number of amino acids: Translated: 446; Mature: 446

Protein sequence:

>446_residues
MNPMKLYDAIIGIVEKHIAPIAAKVGNQPHVRAMRDGFIVAMPFIIVGSFILIFAFPPFAEDTTNVFGRVWLNFATTHFD
IIMMPFNMSMGIMTIFVSLGVAYSLAKAYKMDGITSAVLSLMSFLLVAAPAKEDSLSMAHMGGTGIFTAVMCAFFAVELY
RFMKKHNITIRMPEQVPPAIARSFEVLLPVLAIFLTLYPLSLFVQAEYGMLIPDAVMAMFKPLISASNTLPAIIGALLVC
QLLWFAGIHGAAIVVGLLSPIFLTNISANIDAFVTGQPVPNIFTQPFWDFYIFIGGSGATLALVILMSFSRSAHLKSIGR
MSAVPGFFQINEPVIFGSPVVMNPILFLPFVFAPVINATIAYFAIQLGFVGMGVATTPWTTPAIIGASWGSGWTFTPVLL
VVGLLILDLLIYLPFFKMFEKQVLEQEQPVESKVKAAQPGRQGVTA

Sequences:

>Translated_446_residues
MNPMKLYDAIIGIVEKHIAPIAAKVGNQPHVRAMRDGFIVAMPFIIVGSFILIFAFPPFAEDTTNVFGRVWLNFATTHFD
IIMMPFNMSMGIMTIFVSLGVAYSLAKAYKMDGITSAVLSLMSFLLVAAPAKEDSLSMAHMGGTGIFTAVMCAFFAVELY
RFMKKHNITIRMPEQVPPAIARSFEVLLPVLAIFLTLYPLSLFVQAEYGMLIPDAVMAMFKPLISASNTLPAIIGALLVC
QLLWFAGIHGAAIVVGLLSPIFLTNISANIDAFVTGQPVPNIFTQPFWDFYIFIGGSGATLALVILMSFSRSAHLKSIGR
MSAVPGFFQINEPVIFGSPVVMNPILFLPFVFAPVINATIAYFAIQLGFVGMGVATTPWTTPAIIGASWGSGWTFTPVLL
VVGLLILDLLIYLPFFKMFEKQVLEQEQPVESKVKAAQPGRQGVTA
>Mature_446_residues
MNPMKLYDAIIGIVEKHIAPIAAKVGNQPHVRAMRDGFIVAMPFIIVGSFILIFAFPPFAEDTTNVFGRVWLNFATTHFD
IIMMPFNMSMGIMTIFVSLGVAYSLAKAYKMDGITSAVLSLMSFLLVAAPAKEDSLSMAHMGGTGIFTAVMCAFFAVELY
RFMKKHNITIRMPEQVPPAIARSFEVLLPVLAIFLTLYPLSLFVQAEYGMLIPDAVMAMFKPLISASNTLPAIIGALLVC
QLLWFAGIHGAAIVVGLLSPIFLTNISANIDAFVTGQPVPNIFTQPFWDFYIFIGGSGATLALVILMSFSRSAHLKSIGR
MSAVPGFFQINEPVIFGSPVVMNPILFLPFVFAPVINATIAYFAIQLGFVGMGVATTPWTTPAIIGASWGSGWTFTPVLL
VVGLLILDLLIYLPFFKMFEKQVLEQEQPVESKVKAAQPGRQGVTA

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system i

COG id: COG1455

COG function: function code G; Phosphotransferase system cellobiose-specific component IIC

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-3 domain [H]

Homologues:

Organism=Escherichia coli, GI1788032, Length=453, Percent_Identity=30.0220750551876, Blast_Score=186, Evalue=3e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003352
- InterPro:   IPR004796
- InterPro:   IPR004501 [H]

Pfam domain/function: PF02378 PTS_EIIC [H]

EC number: NA

Molecular weight: Translated: 48528; Mature: 48528

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: PS51105 PTS_EIIC_TYPE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHCCCHHHHHHHCCCCCCCCCC
>Mature Secondary Structure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HHHHHHCCCHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: diacetylchitobiose [Periplasm]; phosphoenolpyruvate; cellobiose [Periplasm] [C]

Specific reaction: phosphoenolpyruvate + diacetylchitobiose [Periplasm] = pyruvate + diacetylchitobiose-6-phosphate [Cytoplasm] phosphoenolpyruvate + cellobiose [Periplasm] = cellobiose-6-phosphate [Cytoplasm] + pyruvate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8990303; 8969509; 9384377 [H]