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The map label for this gene is btuC [H]

Identifier: 227081416

GI number: 227081416

Start: 1273354

End: 1274361

Strand: Direct

Name: btuC [H]

Synonym: VCM66_1199

Alternate gene names: 227081416

Gene position: 1273354-1274361 (Clockwise)

Preceding gene: 227081414

Following gene: 227081417

Centisome position: 44.02

GC content: 52.68

Gene sequence:

>1008_bases
ATGAACGACAAGATGGATTTTCATCGACTGCTTCAACATAAACAGCAACGCTGGCAGCGCAGCAGTTATCTGCTGATCGG
GCTGTTTTTGAGTGTGTGTGTCCTTTATTTGTTGGTGGGAGAGCTGTGGCTATCTCCTTTTTCCGCTTGGTCTTCCCTCG
AACAACAGTTGGTGTGGGAACTGCGTTTGCCAAGATTACTGGCTGCGGCGGTCATTGGGGCTTCTCTGGCCGTAGCGGGA
GCCACCTTACAAGTGTTGCTAGGCAATGTGCTTGCTGAGCCGGGCGTGGTGGGGGTATCGGGTGGTGCAAGCGTGGCGAT
GGTGATTCTGCTGCTGTTTTTCCCGAGTTTAAATTCCCCCGTGGCTTTTATGGCGGCCGCGGTATTGGGCGCGCTGCTGT
TTACGTTGCTCTTGGTGGTGATGGCTCGTAAGTTACGCTTGACCACTGCAAGGCTATTACTGGTCGGGGTCGCATTGGGG
ATTTTGTCTGGCGCGGTAGTCACTTGGGCTTTCTATTTCAGTGATGACCTTGGTTTGCGTCAACTGATGTACTGGTTAAT
GGGCAGTGTCGCAGGCGTCAGTTGGTATCAACATCTCCTCTCTTTGGTGGCAGTACCTGTCGTGATTTGGCTTGTCTTGC
AAGGTGGCGTTTTGGATAAGCTGATGCTTGGTGAAGGGCATGCCAAACAGTTAGGGATCGATATTCATCGTGTTCGCTGG
CGGCTCATTCTCGCTATCGCATTACTGGTCGGGGCTTCCGTCGCACTAGGGGGAGTGATCGGTTTTGTTGGGTTAGTGGT
TCCTCATTTGCTGCGTTTAACGCTAGGCTCGGAAAATCGTCTATTGCTACCACTTTCTGCTCTGTGCGGCGCTTTATTAC
TGGTGAGTGCGGACCTGATTGCGCGTTTGGCGCTGGGCAGCGGCGAATTGCCATTGGGGGTAGTCACCACCACTCTGGGC
GCGCCCATTTTTATCTGGATGTTGGTGAGAAACCATGATTCGTGTTAA

Upstream 100 bases:

>100_bases
GCTATGAAGATGACCAATTGGTGTACGACTGATCTTGCTTGGTCATGATCTCCTTTCTATTTAAATGGCTTAACGGTAAG
GTTAGGCCATTCTTTTTTCT

Downstream 100 bases:

>100_bases
CAGCTTACAGGTGGATTCTCGTCTGCTGCCGTTGTCATTACAGGCTAACGCTGGCGAAGTTTGGCATGTGATTGGCCCCA
ACGGCTGTGGCAAAAGCACC

Product: vtamin B12-transporter permease

Products: ADP; phosphate; cob(I)alamin [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MNDKMDFHRLLQHKQQRWQRSSYLLIGLFLSVCVLYLLVGELWLSPFSAWSSLEQQLVWELRLPRLLAAAVIGASLAVAG
ATLQVLLGNVLAEPGVVGVSGGASVAMVILLLFFPSLNSPVAFMAAAVLGALLFTLLLVVMARKLRLTTARLLLVGVALG
ILSGAVVTWAFYFSDDLGLRQLMYWLMGSVAGVSWYQHLLSLVAVPVVIWLVLQGGVLDKLMLGEGHAKQLGIDIHRVRW
RLILAIALLVGASVALGGVIGFVGLVVPHLLRLTLGSENRLLLPLSALCGALLLVSADLIARLALGSGELPLGVVTTTLG
APIFIWMLVRNHDSC

Sequences:

>Translated_335_residues
MNDKMDFHRLLQHKQQRWQRSSYLLIGLFLSVCVLYLLVGELWLSPFSAWSSLEQQLVWELRLPRLLAAAVIGASLAVAG
ATLQVLLGNVLAEPGVVGVSGGASVAMVILLLFFPSLNSPVAFMAAAVLGALLFTLLLVVMARKLRLTTARLLLVGVALG
ILSGAVVTWAFYFSDDLGLRQLMYWLMGSVAGVSWYQHLLSLVAVPVVIWLVLQGGVLDKLMLGEGHAKQLGIDIHRVRW
RLILAIALLVGASVALGGVIGFVGLVVPHLLRLTLGSENRLLLPLSALCGALLLVSADLIARLALGSGELPLGVVTTTLG
APIFIWMLVRNHDSC
>Mature_335_residues
MNDKMDFHRLLQHKQQRWQRSSYLLIGLFLSVCVLYLLVGELWLSPFSAWSSLEQQLVWELRLPRLLAAAVIGASLAVAG
ATLQVLLGNVLAEPGVVGVSGGASVAMVILLLFFPSLNSPVAFMAAAVLGALLFTLLLVVMARKLRLTTARLLLVGVALG
ILSGAVVTWAFYFSDDLGLRQLMYWLMGSVAGVSWYQHLLSLVAVPVVIWLVLQGGVLDKLMLGEGHAKQLGIDIHRVRW
RLILAIALLVGASVALGGVIGFVGLVVPHLLRLTLGSENRLLLPLSALCGALLLVSADLIARLALGSGELPLGVVTTTLG
APIFIWMLVRNHDSC

Specific function: Part of the ABC transporter complex BtuCDF involved in vitamin B12 import. Involved in the translocation of the substrate across the membrane [H]

COG id: COG4139

COG function: function code H; ABC-type cobalamin transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. FecCD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788004, Length=291, Percent_Identity=49.1408934707904, Blast_Score=228, Evalue=6e-61,
Organism=Escherichia coli, GI1790740, Length=295, Percent_Identity=39.3220338983051, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1790741, Length=283, Percent_Identity=35.3356890459364, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1786805, Length=294, Percent_Identity=34.6938775510204, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1786347, Length=294, Percent_Identity=30.952380952381, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1786804, Length=281, Percent_Identity=30.9608540925267, Blast_Score=82, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000522 [H]

Pfam domain/function: PF01032 FecCD [H]

EC number: NA

Molecular weight: Translated: 35986; Mature: 35986

Theoretical pI: Translated: 9.81; Mature: 9.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDKMDFHRLLQHKQQRWQRSSYLLIGLFLSVCVLYLLVGELWLSPFSAWSSLEQQLVWE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRLPRLLAAAVIGASLAVAGATLQVLLGNVLAEPGVVGVSGGASVAMVILLLFFPSLNSP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHHHHCCCCCC
VAFMAAAVLGALLFTLLLVVMARKLRLTTARLLLVGVALGILSGAVVTWAFYFSDDLGLR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
QLMYWLMGSVAGVSWYQHLLSLVAVPVVIWLVLQGGVLDKLMLGEGHAKQLGIDIHRVRW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHCHHHHHHHH
RLILAIALLVGASVALGGVIGFVGLVVPHLLRLTLGSENRLLLPLSALCGALLLVSADLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHH
ARLALGSGELPLGVVTTTLGAPIFIWMLVRNHDSC
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNDKMDFHRLLQHKQQRWQRSSYLLIGLFLSVCVLYLLVGELWLSPFSAWSSLEQQLVWE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRLPRLLAAAVIGASLAVAGATLQVLLGNVLAEPGVVGVSGGASVAMVILLLFFPSLNSP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHHHHCCCCCC
VAFMAAAVLGALLFTLLLVVMARKLRLTTARLLLVGVALGILSGAVVTWAFYFSDDLGLR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
QLMYWLMGSVAGVSWYQHLLSLVAVPVVIWLVLQGGVLDKLMLGEGHAKQLGIDIHRVRW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHCHHHHHHHH
RLILAIALLVGASVALGGVIGFVGLVVPHLLRLTLGSENRLLLPLSALCGALLLVSADLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHH
ARLALGSGELPLGVVTTTLGAPIFIWMLVRNHDSC
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; cob(I)alamin [Periplasm]; H2O [C]

Specific reaction: ATP + cob(I)alamin [Periplasm] + H2O = ADP + phosphate + cob(I)alamin [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]