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The map label for this gene is astE [H]

Identifier: 227081414

GI number: 227081414

Start: 1272257

End: 1273285

Strand: Direct

Name: astE [H]

Synonym: VCM66_1197

Alternate gene names: 227081414

Gene position: 1272257-1273285 (Clockwise)

Preceding gene: 227081413

Following gene: 227081416

Centisome position: 43.98

GC content: 49.47

Gene sequence:

>1029_bases
ATGACGAAGTCTCTCTTCCGCCAATCTTTTCTTTTCGACAGCTTAGATCTTGACCATCCCATGGTCGCGCAAACCGTACG
TACAGAGCAGGGTGTGACCTTAAAGCTGCATCAACGTGGCGTGTTGGAAGTGATCCCAGCTCAAACGGATGCGGCGACAA
AAAATATGGTGATCTCCTGTGGGATACATGGTGATGAAACCGCACCTATGGAACTGCTGGATAAATGGATTGACGATATC
GTCAGCGGATTTCAACCCGTTGCTGAGCGCTGCTTATTTATTATGGCGCACCCGCAAGCCACGGTGCGCCATGTCCGTTT
TATTGAGCAGAATCTCAACCGCTTATTTGATGATAAGCCCCACACTCCGAGCACTGAGCTGGCGATTGCCGATAACCTCA
AAGTTCTGCTTAGACAGTTTTTTGCCAACACGGATGAACACTCGCGTTGGCATCTAGATTTGCACTGTGCCATTCGTGGC
TCGAAACACTATTCGTTTGCAGTAAGCCCGAAAGCGCGTCATCCGGTGCGCAGCCGTAGCCTGATGCAATTTATCGAGCA
AGCCCATATTGAAGCCGTGATGCTATCGAATGCGCCTTCCAGTACGTTTAGTTGGTATTCAGCCGAGCATTATGCTGCGC
AAGCCTTAACCTTAGAGCTTGGTCAAGTGGCACGCTTAGGTGAAAATCTATTAGACCGTCTGCTGGCCTTTGATCTGGCG
ATGCGAGATCTCATCTCTCGCCATAAACCCGAGCACTTACCGAGAAAATCGGTGATGTACCGAGTCAGTCGCACCATAGT
GCGTCTGCATGATGATTTTGATTTTCGCTTCAGTGACGACGTGGAAAACTTCACCGCCTTTATGCATGGGGAAGTGTTTG
GTCACGATGGTGATAAGCCTTTAATGGCGAAAAATGAAGGGGAAGCGATTGTTTTCCCGAACCGTAAAGTGGCCATTGGC
CAGCGTGCTGCCTTGATGGTGTGTAAGGTGAACACGCGCTATGAAGATGACCAATTGGTGTACGACTGA

Upstream 100 bases:

>100_bases
CGACCACGGATATTGAATGGGTGATGATAAGTGCGATCATCTTGGCATAAATTGTTCGTACAATTTCTTAACATACCGCT
GTGCAATAAAGGAGTAGCGC

Downstream 100 bases:

>100_bases
TCTTGCTTGGTCATGATCTCCTTTCTATTTAAATGGCTTAACGGTAAGGTTAGGCCATTCTTTTTTCTATGAACGACAAG
ATGGATTTTCATCGACTGCT

Product: succinylglutamate desuccinylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 342; Mature: 341

Protein sequence:

>342_residues
MTKSLFRQSFLFDSLDLDHPMVAQTVRTEQGVTLKLHQRGVLEVIPAQTDAATKNMVISCGIHGDETAPMELLDKWIDDI
VSGFQPVAERCLFIMAHPQATVRHVRFIEQNLNRLFDDKPHTPSTELAIADNLKVLLRQFFANTDEHSRWHLDLHCAIRG
SKHYSFAVSPKARHPVRSRSLMQFIEQAHIEAVMLSNAPSSTFSWYSAEHYAAQALTLELGQVARLGENLLDRLLAFDLA
MRDLISRHKPEHLPRKSVMYRVSRTIVRLHDDFDFRFSDDVENFTAFMHGEVFGHDGDKPLMAKNEGEAIVFPNRKVAIG
QRAALMVCKVNTRYEDDQLVYD

Sequences:

>Translated_342_residues
MTKSLFRQSFLFDSLDLDHPMVAQTVRTEQGVTLKLHQRGVLEVIPAQTDAATKNMVISCGIHGDETAPMELLDKWIDDI
VSGFQPVAERCLFIMAHPQATVRHVRFIEQNLNRLFDDKPHTPSTELAIADNLKVLLRQFFANTDEHSRWHLDLHCAIRG
SKHYSFAVSPKARHPVRSRSLMQFIEQAHIEAVMLSNAPSSTFSWYSAEHYAAQALTLELGQVARLGENLLDRLLAFDLA
MRDLISRHKPEHLPRKSVMYRVSRTIVRLHDDFDFRFSDDVENFTAFMHGEVFGHDGDKPLMAKNEGEAIVFPNRKVAIG
QRAALMVCKVNTRYEDDQLVYD
>Mature_341_residues
TKSLFRQSFLFDSLDLDHPMVAQTVRTEQGVTLKLHQRGVLEVIPAQTDAATKNMVISCGIHGDETAPMELLDKWIDDIV
SGFQPVAERCLFIMAHPQATVRHVRFIEQNLNRLFDDKPHTPSTELAIADNLKVLLRQFFANTDEHSRWHLDLHCAIRGS
KHYSFAVSPKARHPVRSRSLMQFIEQAHIEAVMLSNAPSSTFSWYSAEHYAAQALTLELGQVARLGENLLDRLLAFDLAM
RDLISRHKPEHLPRKSVMYRVSRTIVRLHDDFDFRFSDDVENFTAFMHGEVFGHDGDKPLMAKNEGEAIVFPNRKVAIGQ
RAALMVCKVNTRYEDDQLVYD

Specific function: Transforms N(2)-succinylglutamate into succinate and glutamate [H]

COG id: COG2988

COG function: function code E; Succinylglutamate desuccinylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AspA/AstE family. Succinylglutamate desuccinylase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788040, Length=325, Percent_Identity=33.8461538461538, Blast_Score=174, Evalue=9e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007036
- InterPro:   IPR016681 [H]

Pfam domain/function: PF04952 AstE_AspA [H]

EC number: =3.5.1.96 [H]

Molecular weight: Translated: 39093; Mature: 38962

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKSLFRQSFLFDSLDLDHPMVAQTVRTEQGVTLKLHQRGVLEVIPAQTDAATKNMVISC
CCHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCEEEEECCCCEEEECCCCCCCCCCEEEEE
GIHGDETAPMELLDKWIDDIVSGFQPVAERCLFIMAHPQATVRHVRFIEQNLNRLFDDKP
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEECCHHHHHHHHHHHHHHHHHHCCCC
HTPSTELAIADNLKVLLRQFFANTDEHSRWHLDLHCAIRGSKHYSFAVSPKARHPVRSRS
CCCCCCEEEHHHHHHHHHHHHCCCCCCCEEEEEEEEEEECCCCEEEEECCCCCCCHHHHH
LMQFIEQAHIEAVMLSNAPSSTFSWYSAEHYAAQALTLELGQVARLGENLLDRLLAFDLA
HHHHHHHHHHHEEEECCCCCCCCCHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MRDLISRHKPEHLPRKSVMYRVSRTIVRLHDDFDFRFSDDVENFTAFMHGEVFGHDGDKP
HHHHHHHCCCCCCCHHHHHHHHHHHHHEEECCCCCEECCCHHHHHHHHCCEEECCCCCCC
LMAKNEGEAIVFPNRKVAIGQRAALMVCKVNTRYEDDQLVYD
CEECCCCCEEEECCCEEECCCCEEEEEEEECCCCCCCCEECC
>Mature Secondary Structure 
TKSLFRQSFLFDSLDLDHPMVAQTVRTEQGVTLKLHQRGVLEVIPAQTDAATKNMVISC
CHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCEEEEECCCCEEEECCCCCCCCCCEEEEE
GIHGDETAPMELLDKWIDDIVSGFQPVAERCLFIMAHPQATVRHVRFIEQNLNRLFDDKP
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEECCHHHHHHHHHHHHHHHHHHCCCC
HTPSTELAIADNLKVLLRQFFANTDEHSRWHLDLHCAIRGSKHYSFAVSPKARHPVRSRS
CCCCCCEEEHHHHHHHHHHHHCCCCCCCEEEEEEEEEEECCCCEEEEECCCCCCCHHHHH
LMQFIEQAHIEAVMLSNAPSSTFSWYSAEHYAAQALTLELGQVARLGENLLDRLLAFDLA
HHHHHHHHHHHEEEECCCCCCCCCHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MRDLISRHKPEHLPRKSVMYRVSRTIVRLHDDFDFRFSDDVENFTAFMHGEVFGHDGDKP
HHHHHHHCCCCCCCHHHHHHHHHHHHHEEECCCCCEECCCHHHHHHHHCCEEECCCCCCC
LMAKNEGEAIVFPNRKVAIGQRAALMVCKVNTRYEDDQLVYD
CEECCCCCEEEECCCEEECCCCEEEEEEEECCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA