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The map label for this gene is rluB

Identifier: 227081351

GI number: 227081351

Start: 1208301

End: 1209266

Strand: Direct

Name: rluB

Synonym: VCM66_1134

Alternate gene names: 227081351

Gene position: 1208301-1209266 (Clockwise)

Preceding gene: 227081350

Following gene: 227081355

Centisome position: 41.77

GC content: 51.86

Gene sequence:

>966_bases
ATGAGCGAAAAGTTACAAAAAGTTTTAGCACGTGCTGGTCATGGTTCTCGTCGTGAGCTTGAAGCTCTGATCCGAGCTGG
GCGTGTAAGTGTTAATGGCAAGGTAGCTGTACTGGGCGAACGCCTAGAAGATGATAACGCTGTCGTGCGCATCGATGGAC
ATGTTGTGTCTGTGAAAGCGCAAGAAGAGGTGATCTGCCGAGTTCTGGCTTATTACAAACCAGAAGGCGAGCTGTGTACC
CGTCATGATCCAGAAGGTCGCCGTACGGTATTTGACCGTCTGCCGAAAATTCGTGGTTCACGCTGGATTTCTGTTGGCCG
TTTGGACGCCAACACGTCAGGTTTGCTGCTGTTTACCACCGATGGTGAACTGGCTAACCGTTTAATGCACCCAAGCCGTC
AGGTAGAACGTGAATATCTGGTTCGCGTCTTTGGTGACGTGACGGAAGAAAAAGTTCGCAATCTCGTACGTGGTGTGCAG
CTAGAAGATGGTATGGCGCGTTTTGAAGATGTGATGTACGCCGGTGGCGAAGGCATCAACCATACTTTCTACGTGGTAAT
CAATGAAGGTCGTAACCGTGAAGTGCGTCGTCTGTGGGAATCACAAGGTACGACAGTAAGCCGTCTGAAGCGCGTACGTT
ACGGTGATATTTTCCTCGACAAAAAACTGCCTCGCGGCGGTTGGATGGAGCTTGATCTCAAGCAAGTGAACTACCTGCGT
GAGTTAGTAGAACTGCGTCCAGAGAAAGAAACGGTGCTGAACATGGCGCCTGATGCCGCAACGCGTAAGCGTGAGCGTAC
CCGTAGCCAGAAAATTCGTCGTGCAGTACGTCGTCACGAAGAGCGAATTTCAGCTACTCCGGGTCGTAGTGGAAAAGCCC
CTGCGCGCCGTAAGCCGGGTGGTGAATCGAGCACGCGCAATAAAGTGGCTAACCAGCAACAAAGCTCGCGTAAGCCGCGC
GGATAA

Upstream 100 bases:

>100_bases
CGGGTGCTTTTATCCACAAAATTTGCGATACTACGCGGCCGCGATTTTAACATCGCAACCCGTCATTCGACGTCTGAGAA
GACGACACATAGGTAGATAA

Downstream 100 bases:

>100_bases
GTGCCCGCCTTTCTTCGAGCGATGTCGCTTATAAAAAAAGCCAGTCATGTTGACTGGCTTTTTGCTGTTTATTGTTTGAA
GCTGTTTATTGTTTGAAGCT

Product: pseudouridine synthase family 1 protein

Products: NA

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B

Number of amino acids: Translated: 321; Mature: 320

Protein sequence:

>321_residues
MSEKLQKVLARAGHGSRRELEALIRAGRVSVNGKVAVLGERLEDDNAVVRIDGHVVSVKAQEEVICRVLAYYKPEGELCT
RHDPEGRRTVFDRLPKIRGSRWISVGRLDANTSGLLLFTTDGELANRLMHPSRQVEREYLVRVFGDVTEEKVRNLVRGVQ
LEDGMARFEDVMYAGGEGINHTFYVVINEGRNREVRRLWESQGTTVSRLKRVRYGDIFLDKKLPRGGWMELDLKQVNYLR
ELVELRPEKETVLNMAPDAATRKRERTRSQKIRRAVRRHEERISATPGRSGKAPARRKPGGESSTRNKVANQQQSSRKPR
G

Sequences:

>Translated_321_residues
MSEKLQKVLARAGHGSRRELEALIRAGRVSVNGKVAVLGERLEDDNAVVRIDGHVVSVKAQEEVICRVLAYYKPEGELCT
RHDPEGRRTVFDRLPKIRGSRWISVGRLDANTSGLLLFTTDGELANRLMHPSRQVEREYLVRVFGDVTEEKVRNLVRGVQ
LEDGMARFEDVMYAGGEGINHTFYVVINEGRNREVRRLWESQGTTVSRLKRVRYGDIFLDKKLPRGGWMELDLKQVNYLR
ELVELRPEKETVLNMAPDAATRKRERTRSQKIRRAVRRHEERISATPGRSGKAPARRKPGGESSTRNKVANQQQSSRKPR
G
>Mature_320_residues
SEKLQKVLARAGHGSRRELEALIRAGRVSVNGKVAVLGERLEDDNAVVRIDGHVVSVKAQEEVICRVLAYYKPEGELCTR
HDPEGRRTVFDRLPKIRGSRWISVGRLDANTSGLLLFTTDGELANRLMHPSRQVEREYLVRVFGDVTEEKVRNLVRGVQL
EDGMARFEDVMYAGGEGINHTFYVVINEGRNREVRRLWESQGTTVSRLKRVRYGDIFLDKKLPRGGWMELDLKQVNYLRE
LVELRPEKETVLNMAPDAATRKRERTRSQKIRRAVRRHEERISATPGRSGKAPARRKPGGESSTRNKVANQQQSSRKPRG

Specific function: Responsible for synthesis of pseudouridine from uracil- 2605 in 23S ribosomal RNA

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain

Homologues:

Organism=Escherichia coli, GI1787524, Length=282, Percent_Identity=68.4397163120567, Blast_Score=387, Evalue=1e-109,
Organism=Escherichia coli, GI1790453, Length=304, Percent_Identity=27.6315789473684, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1788510, Length=212, Percent_Identity=30.6603773584906, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI87081838, Length=207, Percent_Identity=29.4685990338164, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLUB_VIBCH (Q9KSS7)

Other databases:

- EMBL:   AE003852
- PIR:   H82230
- RefSeq:   NP_230824.1
- ProteinModelPortal:   Q9KSS7
- SMR:   Q9KSS7
- GeneID:   2614612
- GenomeReviews:   AE003852_GR
- KEGG:   vch:VC1179
- TIGR:   VC_1179
- HOGENOM:   HBG754478
- OMA:   ELCTRSD
- ProtClustDB:   CLSK874246
- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942
- SMART:   SM00363
- TIGRFAMs:   TIGR00093

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4; SSF55120 PsdUridine_synth_cat_dom

EC number: NA

Molecular weight: Translated: 36657; Mature: 36526

Theoretical pI: Translated: 10.95; Mature: 10.95

Prosite motif: PS01149 PSI_RSU; PS50889 S4

Important sites: ACT_SITE 109-109

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKLQKVLARAGHGSRRELEALIRAGRVSVNGKVAVLGERLEDDNAVVRIDGHVVSVKA
CCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEECCEEEEEEC
QEEVICRVLAYYKPEGELCTRHDPEGRRTVFDRLPKIRGSRWISVGRLDANTSGLLLFTT
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEEEEC
DGELANRLMHPSRQVEREYLVRVFGDVTEEKVRNLVRGVQLEDGMARFEDVMYAGGEGIN
CHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCC
HTFYVVINEGRNREVRRLWESQGTTVSRLKRVRYGDIFLDKKLPRGGWMELDLKQVNYLR
EEEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHCCEEEECCCCCCCCEEEHHHHHHHHH
ELVELRPEKETVLNMAPDAATRKRERTRSQKIRRAVRRHEERISATPGRSGKAPARRKPG
HHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
GESSTRNKVANQQQSSRKPRG
CCCHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SEKLQKVLARAGHGSRRELEALIRAGRVSVNGKVAVLGERLEDDNAVVRIDGHVVSVKA
CHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEECCEEEEEEC
QEEVICRVLAYYKPEGELCTRHDPEGRRTVFDRLPKIRGSRWISVGRLDANTSGLLLFTT
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEEEEC
DGELANRLMHPSRQVEREYLVRVFGDVTEEKVRNLVRGVQLEDGMARFEDVMYAGGEGIN
CHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCC
HTFYVVINEGRNREVRRLWESQGTTVSRLKRVRYGDIFLDKKLPRGGWMELDLKQVNYLR
EEEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHCCEEEECCCCCCCCEEEHHHHHHHHH
ELVELRPEKETVLNMAPDAATRKRERTRSQKIRRAVRRHEERISATPGRSGKAPARRKPG
HHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
GESSTRNKVANQQQSSRKPRG
CCCHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301