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The map label for this gene is cmoB

Identifier: 227081335

GI number: 227081335

Start: 1192453

End: 1193424

Strand: Reverse

Name: cmoB

Synonym: VCM66_1118

Alternate gene names: 227081335

Gene position: 1193424-1192453 (Counterclockwise)

Preceding gene: 227081336

Following gene: 227081334

Centisome position: 41.26

GC content: 50.62

Gene sequence:

>972_bases
ATGTTCAACTTTGCCAACGTTTACCAACAGATCGCTCAACATCCGCAGTTACAGTTGTGGCTCAACACCCTGCCTCAGCA
ATTGACGGATTGGCAAGCTAAGCAGCACGGAGATCTCGATCGCTGGATGCGTAACCTCAAGAAAATTCCGGTCGGTCAAC
CGGAGGTGATTGATCTGAAAAATGCGGTAGCGGCACACAATCATCAGCCACTCGCGCAAGGTGAACAGAAGAAATTGGAA
GCGGTGCTCAAAACCTTTCACCCTTGGCGTAAAGGTCCTTACCATCTGCACGGCATTCATATTGATACCGAATGGCGATC
CGATTGGAAATGGGATCGCTTACTGCCACACATCTCGCCACTGAAAAACCGTTTGGTACTTGATGTGGGTTGTGGCAACG
GCTATCACATGTGGCGCATGCTGGGTGAAGGGGCGCAGCAAGTGTTCGGCATTGACCCTTCCGAGCTGTTTCTGATCCAG
TTTGAAGCGGTACGTAAACTGCTGGGCGATGACCAACGCGTTCATCTCTTGCCACTCGGTATTGAACAGATGCCGGAGCT
CAACGCCTTTGATACTGTGTTCAGCATGGGCGTGCTTTACCACCGTCGCTCACCACTCGATCACCTGCTGCAACTGAAAA
ATCAGTTGGTTGCTGGCGGTGAATTGATCCTGGAAACCTTAGTCGTAGAAGGGGATGAACATACGGTACTGGTGCCGTTT
GATCGCTACGCCCAAATGCGCAACGTCTATTTCTTCCCTTCTGCACTGGCTCTTAAAGTGTGGTTAGAAAAGACAGGGTT
TGTCGATGTGCGTATTGTCGATGAAAATATCACTTCACTCGGTGAGCAGCGTACCACAGAGTGGATGACACACAATTCAC
TGCCCGATTACGTTGACCCACAAGACCCAAGCAAGACCATTGAAGGCTATCCGGCGCCGCGACGTGCCATTCTGATTGCC
AAAAAGCCATAA

Upstream 100 bases:

>100_bases
TGTTAACTATTTTTGTCATTTATCTTCATGTTAGCGACTCTCTGCGCCACGTATGATCCTGAAGTCATGTGCAGAGAGCA
TAGTTTAGGTATTTAGCCCC

Downstream 100 bases:

>100_bases
TTGACACAGTTTTAACTCGCTTATTCGGGTATCACGTTACCCTTGGTAAAGGATGCGCTAAGCATCCTTTGCTATAACTT
ATCTATACAATCACTCATTA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MFNFANVYQQIAQHPQLQLWLNTLPQQLTDWQAKQHGDLDRWMRNLKKIPVGQPEVIDLKNAVAAHNHQPLAQGEQKKLE
AVLKTFHPWRKGPYHLHGIHIDTEWRSDWKWDRLLPHISPLKNRLVLDVGCGNGYHMWRMLGEGAQQVFGIDPSELFLIQ
FEAVRKLLGDDQRVHLLPLGIEQMPELNAFDTVFSMGVLYHRRSPLDHLLQLKNQLVAGGELILETLVVEGDEHTVLVPF
DRYAQMRNVYFFPSALALKVWLEKTGFVDVRIVDENITSLGEQRTTEWMTHNSLPDYVDPQDPSKTIEGYPAPRRAILIA
KKP

Sequences:

>Translated_323_residues
MFNFANVYQQIAQHPQLQLWLNTLPQQLTDWQAKQHGDLDRWMRNLKKIPVGQPEVIDLKNAVAAHNHQPLAQGEQKKLE
AVLKTFHPWRKGPYHLHGIHIDTEWRSDWKWDRLLPHISPLKNRLVLDVGCGNGYHMWRMLGEGAQQVFGIDPSELFLIQ
FEAVRKLLGDDQRVHLLPLGIEQMPELNAFDTVFSMGVLYHRRSPLDHLLQLKNQLVAGGELILETLVVEGDEHTVLVPF
DRYAQMRNVYFFPSALALKVWLEKTGFVDVRIVDENITSLGEQRTTEWMTHNSLPDYVDPQDPSKTIEGYPAPRRAILIA
KKP
>Mature_323_residues
MFNFANVYQQIAQHPQLQLWLNTLPQQLTDWQAKQHGDLDRWMRNLKKIPVGQPEVIDLKNAVAAHNHQPLAQGEQKKLE
AVLKTFHPWRKGPYHLHGIHIDTEWRSDWKWDRLLPHISPLKNRLVLDVGCGNGYHMWRMLGEGAQQVFGIDPSELFLIQ
FEAVRKLLGDDQRVHLLPLGIEQMPELNAFDTVFSMGVLYHRRSPLDHLLQLKNQLVAGGELILETLVVEGDEHTVLVPF
DRYAQMRNVYFFPSALALKVWLEKTGFVDVRIVDENITSLGEQRTTEWMTHNSLPDYVDPQDPSKTIEGYPAPRRAILIA
KKP

Specific function: Catalyzes the conversion of 5-hydroxyuridine (ho5U) to 5-methoxyuridine (mo5U) at position 34 in tRNA

COG id: COG0500

COG function: function code QR; SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. CmoB family

Homologues:

Organism=Escherichia coli, GI1788178, Length=324, Percent_Identity=62.962962962963, Blast_Score=437, Evalue=1e-124,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CMOB_VIBC3 (A5F277)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F277
- SMR:   A5F277
- STRING:   A5F277
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A0732
- eggNOG:   COG0500
- HOGENOM:   HBG296882
- OMA:   DVIFCLG
- ProtClustDB:   PRK15068
- BioCyc:   VCHO345073:VC0395_A0732-MONOMER
- HAMAP:   MF_01590
- InterPro:   IPR010017
- TIGRFAMs:   TIGR00452

Pfam domain/function: PF08003 Methyltransf_9

EC number: NA

Molecular weight: Translated: 37340; Mature: 37340

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNFANVYQQIAQHPQLQLWLNTLPQQLTDWQAKQHGDLDRWMRNLKKIPVGQPEVIDLK
CCCHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCEEEHH
NAVAAHNHQPLAQGEQKKLEAVLKTFHPWRKGPYHLHGIHIDTEWRSDWKWDRLLPHISP
HHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCCHHHHCCCHHC
LKNRLVLDVGCGNGYHMWRMLGEGAQQVFGIDPSELFLIQFEAVRKLLGDDQRVHLLPLG
CCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHEEEEEHHHHHHHHCCCCEEEEEECC
IEQMPELNAFDTVFSMGVLYHRRSPLDHLLQLKNQLVAGGELILETLVVEGDEHTVLVPF
HHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHEECCCCEEEEEH
DRYAQMRNVYFFPSALALKVWLEKTGFVDVRIVDENITSLGEQRTTEWMTHNSLPDYVDP
HHHHHHCCEEECCHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCC
QDPSKTIEGYPAPRRAILIAKKP
CCCCCCCCCCCCCCEEEEEEECC
>Mature Secondary Structure
MFNFANVYQQIAQHPQLQLWLNTLPQQLTDWQAKQHGDLDRWMRNLKKIPVGQPEVIDLK
CCCHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCEEEHH
NAVAAHNHQPLAQGEQKKLEAVLKTFHPWRKGPYHLHGIHIDTEWRSDWKWDRLLPHISP
HHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCCHHHHCCCHHC
LKNRLVLDVGCGNGYHMWRMLGEGAQQVFGIDPSELFLIQFEAVRKLLGDDQRVHLLPLG
CCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHEEEEEHHHHHHHHCCCCEEEEEECC
IEQMPELNAFDTVFSMGVLYHRRSPLDHLLQLKNQLVAGGELILETLVVEGDEHTVLVPF
HHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHEECCCCEEEEEH
DRYAQMRNVYFFPSALALKVWLEKTGFVDVRIVDENITSLGEQRTTEWMTHNSLPDYVDP
HHHHHHCCEEECCHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCC
QDPSKTIEGYPAPRRAILIAKKP
CCCCCCCCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA