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The map label for this gene is dusC

Identifier: 227081278

GI number: 227081278

Start: 1132641

End: 1133612

Strand: Reverse

Name: dusC

Synonym: VCM66_1061

Alternate gene names: 227081278

Gene position: 1133612-1132641 (Counterclockwise)

Preceding gene: 227081284

Following gene: 227081273

Centisome position: 39.19

GC content: 50.93

Gene sequence:

>972_bases
ATGCGAGTTATTTTAGGCCCGATGGAGGGCGTGTTAGATCATTTGATGCGTGACATGCTGACACAAATCAATGACTACGA
TTTCTGCGTCACCGAATTTGTACGCGTAGTGAATCTGCTGCTGCCTGATCATGTGTTTTATCGCCTCTGTCCTGAATTGC
AACAGGGTTCTAAAACTCCGTCCGGTGTTCCGGTAAAAGTACAACTGCTTGGCCAAGACCCACACTGGATGGCCGAAAAC
GCGATCCGCGCTGCTGAGCTTGGGGCTTATGGCATCGATCTAAATTTCGGCTGCCCTGCCAAAATGGTCAATCAGAGCAA
AGGTGGCGCGGCTCTTTTGCAGCATCCCGAGTTGATTTATCAGGTGGTCAAAGCGTGTCGTGACGCAGTACCGGCGCACA
TTCCTGTTTCGGCGAAAATCCGCTTAGGTTGGGAAGATCCGGAAGATTGTTTTGAGATTGTCGATGCCGTTGCGCAAGCT
AAAGCCGATGAGCTCACTGTGCACGCGCGCACCAAAGCCGGAGGCTACAAGGCCAGCGAAATCAAATGGCATTACATTGA
CCAGATCCGCCAAAAATGCAGTATTCCATTGATTGCTAACGGTGAAGTGTGGAATTACGCCGATGGTCAAGCTTGCATCC
AAACCACAGGTGTCGATTCACTCATGGTGTGCCGCGGCGCGCTCAACGTGCCGAATTTAGGCAATGTGGTCAAACACAAT
CACGCCGCCATGCCTTGGCATGAAGTGGTGGATTTGTTGCTGCGCTACACGCAATTTGAAGTCCGTGGTGACAAAGGCAA
ATACTACCCAAACCGTATTAAGCAGTGGTTTGCTTATCTGCGCCACGCCTACCCTCAGGCTAATGAGCTGTTTGGTGAGT
TACGTACGTTGACTCAAGTCGAGCCGATCGTTGATCAGTTACATCGCTATCGCGATCAACTCCATCATGCTGCGGCCGAG
CTACCTGTCTAA

Upstream 100 bases:

>100_bases
CGTCACACTGAACTTGAATCAGAAAGGATGAGTGACATTTATCGATGCTACTACTACAATCTCGCTCCCTTTTCTTCAGC
GCTAGCCGGTATTCATCACT

Downstream 100 bases:

>100_bases
TGAGTAAAACAACGAATCAGTAAATCAACTGATCAGTAAACCAACTGATTTTTGCCGCTGGTTTTCGCTACATACAACTT
TTCATCAGCGGCTTTAAACA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MRVILGPMEGVLDHLMRDMLTQINDYDFCVTEFVRVVNLLLPDHVFYRLCPELQQGSKTPSGVPVKVQLLGQDPHWMAEN
AIRAAELGAYGIDLNFGCPAKMVNQSKGGAALLQHPELIYQVVKACRDAVPAHIPVSAKIRLGWEDPEDCFEIVDAVAQA
KADELTVHARTKAGGYKASEIKWHYIDQIRQKCSIPLIANGEVWNYADGQACIQTTGVDSLMVCRGALNVPNLGNVVKHN
HAAMPWHEVVDLLLRYTQFEVRGDKGKYYPNRIKQWFAYLRHAYPQANELFGELRTLTQVEPIVDQLHRYRDQLHHAAAE
LPV

Sequences:

>Translated_323_residues
MRVILGPMEGVLDHLMRDMLTQINDYDFCVTEFVRVVNLLLPDHVFYRLCPELQQGSKTPSGVPVKVQLLGQDPHWMAEN
AIRAAELGAYGIDLNFGCPAKMVNQSKGGAALLQHPELIYQVVKACRDAVPAHIPVSAKIRLGWEDPEDCFEIVDAVAQA
KADELTVHARTKAGGYKASEIKWHYIDQIRQKCSIPLIANGEVWNYADGQACIQTTGVDSLMVCRGALNVPNLGNVVKHN
HAAMPWHEVVDLLLRYTQFEVRGDKGKYYPNRIKQWFAYLRHAYPQANELFGELRTLTQVEPIVDQLHRYRDQLHHAAAE
LPV
>Mature_323_residues
MRVILGPMEGVLDHLMRDMLTQINDYDFCVTEFVRVVNLLLPDHVFYRLCPELQQGSKTPSGVPVKVQLLGQDPHWMAEN
AIRAAELGAYGIDLNFGCPAKMVNQSKGGAALLQHPELIYQVVKACRDAVPAHIPVSAKIRLGWEDPEDCFEIVDAVAQA
KADELTVHARTKAGGYKASEIKWHYIDQIRQKCSIPLIANGEVWNYADGQACIQTTGVDSLMVCRGALNVPNLGNVVKHN
HAAMPWHEVVDLLLRYTQFEVRGDKGKYYPNRIKQWFAYLRHAYPQANELFGELRTLTQVEPIVDQLHRYRDQLHHAAAE
LPV

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusC subfamily

Homologues:

Organism=Homo sapiens, GI40807366, Length=202, Percent_Identity=28.2178217821782, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI31742496, Length=174, Percent_Identity=32.7586206896552, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI239788483, Length=199, Percent_Identity=29.1457286432161, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI239788462, Length=199, Percent_Identity=29.1457286432161, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI8923374, Length=235, Percent_Identity=28.5106382978723, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1788462, Length=298, Percent_Identity=60.4026845637584, Blast_Score=386, Evalue=1e-108,
Organism=Escherichia coli, GI1789660, Length=241, Percent_Identity=33.6099585062241, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI145693211, Length=182, Percent_Identity=29.1208791208791, Blast_Score=65, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI25144369, Length=218, Percent_Identity=29.8165137614679, Blast_Score=87, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17510279, Length=276, Percent_Identity=27.536231884058, Blast_Score=81, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17507177, Length=154, Percent_Identity=31.8181818181818, Blast_Score=71, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6323560, Length=239, Percent_Identity=25.9414225941423, Blast_Score=78, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323437, Length=160, Percent_Identity=28.75, Blast_Score=64, Evalue=3e-11,
Organism=Drosophila melanogaster, GI19921524, Length=176, Percent_Identity=34.0909090909091, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24580595, Length=175, Percent_Identity=30.2857142857143, Blast_Score=84, Evalue=9e-17,
Organism=Drosophila melanogaster, GI19920448, Length=175, Percent_Identity=30.2857142857143, Blast_Score=84, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24585320, Length=232, Percent_Identity=27.5862068965517, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DUSC_VIBCH (Q9KT00)

Other databases:

- EMBL:   AE003852
- PIR:   D82241
- RefSeq:   NP_230750.1
- ProteinModelPortal:   Q9KT00
- SMR:   Q9KT00
- GeneID:   2614375
- GenomeReviews:   AE003852_GR
- KEGG:   vch:VC1105
- TIGR:   VC_1105
- HOGENOM:   HBG557545
- OMA:   VGGIDWC
- ProtClustDB:   CLSK874217
- InterPro:   IPR013785
- InterPro:   IPR001269
- InterPro:   IPR018517
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR11082
- PIRSF:   PIRSF006621

Pfam domain/function: PF01207 Dus

EC number: NA

Molecular weight: Translated: 36411; Mature: 36411

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVILGPMEGVLDHLMRDMLTQINDYDFCVTEFVRVVNLLLPDHVFYRLCPELQQGSKTP
CCEEECHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCC
SGVPVKVQLLGQDPHWMAENAIRAAELGAYGIDLNFGCPAKMVNQSKGGAALLQHPELIY
CCCCEEEEEECCCCCHHHHHHHHHHHCCCEEEECCCCCCHHHHCCCCCCHHHHCCHHHHH
QVVKACRDAVPAHIPVSAKIRLGWEDPEDCFEIVDAVAQAKADELTVHARTKAGGYKASE
HHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCHHHEEEEEECCCCCCCCCH
IKWHYIDQIRQKCSIPLIANGEVWNYADGQACIQTTGVDSLMVCRGALNVPNLGNVVKHN
HHHHHHHHHHHHCCCCEEECCCEECCCCCCHHHHHCCCCHHHHHHCCCCCCCCCHHHHCC
HAAMPWHEVVDLLLRYTQFEVRGDKGKYYPNRIKQWFAYLRHAYPQANELFGELRTLTQV
CCCCCHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
EPIVDQLHRYRDQLHHAAAELPV
HHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRVILGPMEGVLDHLMRDMLTQINDYDFCVTEFVRVVNLLLPDHVFYRLCPELQQGSKTP
CCEEECHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCC
SGVPVKVQLLGQDPHWMAENAIRAAELGAYGIDLNFGCPAKMVNQSKGGAALLQHPELIY
CCCCEEEEEECCCCCHHHHHHHHHHHCCCEEEECCCCCCHHHHCCCCCCHHHHCCHHHHH
QVVKACRDAVPAHIPVSAKIRLGWEDPEDCFEIVDAVAQAKADELTVHARTKAGGYKASE
HHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCHHHEEEEEECCCCCCCCCH
IKWHYIDQIRQKCSIPLIANGEVWNYADGQACIQTTGVDSLMVCRGALNVPNLGNVVKHN
HHHHHHHHHHHHCCCCEEECCCEECCCCCCHHHHHCCCCHHHHHHCCCCCCCCCHHHHCC
HAAMPWHEVVDLLLRYTQFEVRGDKGKYYPNRIKQWFAYLRHAYPQANELFGELRTLTQV
CCCCCHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
EPIVDQLHRYRDQLHHAAAELPV
HHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301