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The map label for this gene is metG [H]

Identifier: 227081209

GI number: 227081209

Start: 1059797

End: 1061992

Strand: Reverse

Name: metG [H]

Synonym: VCM66_0991

Alternate gene names: 227081209

Gene position: 1061992-1059797 (Counterclockwise)

Preceding gene: 227081213

Following gene: 227081208

Centisome position: 36.72

GC content: 48.86

Gene sequence:

>2196_bases
TTGCCCATGCTACCACCGCTCAAAGAGCAACTGAACCCCGAAAAAGATAGGGGAATCAACTGTTGCGCGCCTTGGCGTGA
CTGCGTGCTTAGGGTAGTATTATCGCTTCAATTTGGTCTCTATCAAGAAAAGCGAATATTAAGTATGGCAAACGATCCAA
GAAAACTTCTGGTAACTTGTGCCCTGCCTTACGCGAACGGTTCAATCCACCTTGGTCATATGCTTGAGCATATCCAAGCT
GACATTTGGGTTCGTTATCAGCGTCTGCGTGGCAATACAGTAAACTTCATCTGTGCGGATGATGCGCACGGCACACCTAT
CATGCTGAAAGCACAGCAAATGGGTATGACACCAGAAGCGATGATCGAGATGGTGAGCGAAGAGCACCAACGTGACTTTG
CTGGCTTCGATATTAGCTTTGATAACTACCACAGCACGCACAGCGATGAAAACCGCGAGCTGGCTTCGCACATCTATCTG
CAACTGAAAAAGAACGGCTTTATTTCAAGCCGCACCATTTCTCAGCTGTTCGATCCTGAAAAAGAGATGTTCTTGCCGGA
TCGTTTCGTAAAAGGCACTTGCCCGAAATGTAAATCGGAAGACCAGTACGGCGACAACTGTGACGCGTGTGGTGAGACTT
ACAGCCCGACTGAGCTGATCAATCCAAAATCCGCCGTCTCTGGTGCGACGCCAGTGATGAAAGATTCTGAGCACTTTTTC
TTTGACCTGCCTCAGTTTGAAAGCATGCTCAAAGAGTGGACACGTTCAGGCTCTCTGCAATCAGAAACCGCCAACAAAAT
GCAAGAGTGGTTTGAAGGCGGCTTGCAGCAGTGGGATATTTCCCGCGATGCACCGTACTTTGGTTTTGAAATCCCGGGCG
AGAAAGACAAATTCTTCTACGTATGGCTTGATGCCCCGATTGGTTACATGGGTTCATTCAAAAACCTGTGCGACAAGCGT
GGCGATTTGGACTTTAACGAATACTGGAATAAAGACAGCAAAACCGAGCTTTACCATTTCATCGGTAAAGACATCGTTTA
TTTCCACAGCTTGTTCTGGCCTGCCATGCTCGATGGCTCTGGTTTCCGTAAACCGACCAACGTGTTTGTGCATGGTTATG
TGACCGTCAATGGCGCGAAAATGTCTAAGTCGAAAGGTACGTTTGTGAAAGCCAGCACTTACCTGAATCACTTGGATCCA
GAGTGTCTGCGTTACTACTACGCGGCAAAACTGAATAACCGCATTGATGATCTCGATTTGAACCTCGAAGACTTCACCCA
ACGTGTGAATGCGGATGTGGTGAACAAGATCGTAAACCTTGCGTCACGTAATGCAGGTTTCATCACTAAACGTTTTGATG
GCAAGCTGTCTGCACACTTTGCGGAGCCTGAGCTGTACGCTGAATTTGCTGGCGCAGCCGATCGTATTGCTGAGCTGTTT
GAAGCACGTGAGTTTGGTCGTGCCATCCGTGAAATTACTGCATTGGCAGATAAAGCCAACCAGTATGTGGATGAAAAAGC
CCCTTGGGTTGTCGCGAAACAAGAAGGCCAAGACCAAGCACTGCAAGACATCTGTACGGTAGGCATTAACCTATTCCGCG
TACTGATGACTTACCTCAAGCCAGTGATGCCTGCGTTGGCAGAGCGTACTGAAGCTTTCCTTAACCAAGAATTGACTTGG
GAAGGCGTGGCAACTCCGCTAACGGATCATGCGGTAACGCCATTCAAAGCACTGTTCAACCGTATCGATCCGAAACAGGT
TGAAGCTATGATTGAGGCGTCGAAAGCCGAGGCGGCAGCCGAAAAAGCCGCAGCAGATGCCGCAAAACCAAAGAGCGCAG
AAACCGAGCTGAGCAAAGACCCCCTAGCGGCGGAGATTGAGTTTGATGATTTTGCGAAAGTGGATCTGCGGATTGCGAAG
ATTCTTTCTTGTGAAGCGGTCGAGAAATCGGACAAGCTGCTCAAGTTTGAACTCGATATCGGCGGTGAAACCCGTCAAGT
CTTCTCTGGCATTAAGTCTGCCTACCAACCAGAAGATCTGATTGGTAAATACACTGTCGTCGTAGCAAACCTCAAACCGC
GTAAGATGAAGTTTGGTATGTCTGAAGGCATGATCCTCGCCGCAGGCCCTGGCGGTAGCGACCTGTGGTTACTTGAACCC
CATCAAGGCGCTCAAGCGGGTATGCGTGTGATGTAA

Upstream 100 bases:

>100_bases
CGACATCGCCCAATCACTGATTAAATCGGGATGCGAAAATTGGCTCAGCCAGCGGCAGAGATCCTGTTTGGATTGAATAG
ATTGCATAACTTCCCCTTTT

Downstream 100 bases:

>100_bases
TTATTGCGTCTTGATTAGCAAGCCAACACCTGAAAAGCCTTTGCATCTGCAAAGGCTTTTTTGTCATCACCCGCTTTCTC
AAGCCAAACATTTTCTCTTC

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 731; Mature: 730

Protein sequence:

>731_residues
MPMLPPLKEQLNPEKDRGINCCAPWRDCVLRVVLSLQFGLYQEKRILSMANDPRKLLVTCALPYANGSIHLGHMLEHIQA
DIWVRYQRLRGNTVNFICADDAHGTPIMLKAQQMGMTPEAMIEMVSEEHQRDFAGFDISFDNYHSTHSDENRELASHIYL
QLKKNGFISSRTISQLFDPEKEMFLPDRFVKGTCPKCKSEDQYGDNCDACGETYSPTELINPKSAVSGATPVMKDSEHFF
FDLPQFESMLKEWTRSGSLQSETANKMQEWFEGGLQQWDISRDAPYFGFEIPGEKDKFFYVWLDAPIGYMGSFKNLCDKR
GDLDFNEYWNKDSKTELYHFIGKDIVYFHSLFWPAMLDGSGFRKPTNVFVHGYVTVNGAKMSKSKGTFVKASTYLNHLDP
ECLRYYYAAKLNNRIDDLDLNLEDFTQRVNADVVNKIVNLASRNAGFITKRFDGKLSAHFAEPELYAEFAGAADRIAELF
EAREFGRAIREITALADKANQYVDEKAPWVVAKQEGQDQALQDICTVGINLFRVLMTYLKPVMPALAERTEAFLNQELTW
EGVATPLTDHAVTPFKALFNRIDPKQVEAMIEASKAEAAAEKAAADAAKPKSAETELSKDPLAAEIEFDDFAKVDLRIAK
ILSCEAVEKSDKLLKFELDIGGETRQVFSGIKSAYQPEDLIGKYTVVVANLKPRKMKFGMSEGMILAAGPGGSDLWLLEP
HQGAQAGMRVM

Sequences:

>Translated_731_residues
MPMLPPLKEQLNPEKDRGINCCAPWRDCVLRVVLSLQFGLYQEKRILSMANDPRKLLVTCALPYANGSIHLGHMLEHIQA
DIWVRYQRLRGNTVNFICADDAHGTPIMLKAQQMGMTPEAMIEMVSEEHQRDFAGFDISFDNYHSTHSDENRELASHIYL
QLKKNGFISSRTISQLFDPEKEMFLPDRFVKGTCPKCKSEDQYGDNCDACGETYSPTELINPKSAVSGATPVMKDSEHFF
FDLPQFESMLKEWTRSGSLQSETANKMQEWFEGGLQQWDISRDAPYFGFEIPGEKDKFFYVWLDAPIGYMGSFKNLCDKR
GDLDFNEYWNKDSKTELYHFIGKDIVYFHSLFWPAMLDGSGFRKPTNVFVHGYVTVNGAKMSKSKGTFVKASTYLNHLDP
ECLRYYYAAKLNNRIDDLDLNLEDFTQRVNADVVNKIVNLASRNAGFITKRFDGKLSAHFAEPELYAEFAGAADRIAELF
EAREFGRAIREITALADKANQYVDEKAPWVVAKQEGQDQALQDICTVGINLFRVLMTYLKPVMPALAERTEAFLNQELTW
EGVATPLTDHAVTPFKALFNRIDPKQVEAMIEASKAEAAAEKAAADAAKPKSAETELSKDPLAAEIEFDDFAKVDLRIAK
ILSCEAVEKSDKLLKFELDIGGETRQVFSGIKSAYQPEDLIGKYTVVVANLKPRKMKFGMSEGMILAAGPGGSDLWLLEP
HQGAQAGMRVM
>Mature_730_residues
PMLPPLKEQLNPEKDRGINCCAPWRDCVLRVVLSLQFGLYQEKRILSMANDPRKLLVTCALPYANGSIHLGHMLEHIQAD
IWVRYQRLRGNTVNFICADDAHGTPIMLKAQQMGMTPEAMIEMVSEEHQRDFAGFDISFDNYHSTHSDENRELASHIYLQ
LKKNGFISSRTISQLFDPEKEMFLPDRFVKGTCPKCKSEDQYGDNCDACGETYSPTELINPKSAVSGATPVMKDSEHFFF
DLPQFESMLKEWTRSGSLQSETANKMQEWFEGGLQQWDISRDAPYFGFEIPGEKDKFFYVWLDAPIGYMGSFKNLCDKRG
DLDFNEYWNKDSKTELYHFIGKDIVYFHSLFWPAMLDGSGFRKPTNVFVHGYVTVNGAKMSKSKGTFVKASTYLNHLDPE
CLRYYYAAKLNNRIDDLDLNLEDFTQRVNADVVNKIVNLASRNAGFITKRFDGKLSAHFAEPELYAEFAGAADRIAELFE
AREFGRAIREITALADKANQYVDEKAPWVVAKQEGQDQALQDICTVGINLFRVLMTYLKPVMPALAERTEAFLNQELTWE
GVATPLTDHAVTPFKALFNRIDPKQVEAMIEASKAEAAAEKAAADAAKPKSAETELSKDPLAAEIEFDDFAKVDLRIAKI
LSCEAVEKSDKLLKFELDIGGETRQVFSGIKSAYQPEDLIGKYTVVVANLKPRKMKFGMSEGMILAAGPGGSDLWLLEPH
QGAQAGMRVM

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI14043022, Length=613, Percent_Identity=28.8743882544861, Blast_Score=266, Evalue=5e-71,
Organism=Homo sapiens, GI24308436, Length=521, Percent_Identity=26.295585412668, Blast_Score=178, Evalue=2e-44,
Organism=Escherichia coli, GI1788432, Length=684, Percent_Identity=72.8070175438597, Blast_Score=1064, Evalue=0.0,
Organism=Escherichia coli, GI1789455, Length=107, Percent_Identity=36.4485981308411, Blast_Score=73, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17540794, Length=632, Percent_Identity=28.7974683544304, Blast_Score=256, Evalue=4e-68,
Organism=Caenorhabditis elegans, GI212645096, Length=512, Percent_Identity=24.609375, Blast_Score=155, Evalue=5e-38,
Organism=Caenorhabditis elegans, GI71992781, Length=407, Percent_Identity=23.8329238329238, Blast_Score=112, Evalue=7e-25,
Organism=Saccharomyces cerevisiae, GI6321704, Length=567, Percent_Identity=29.2768959435626, Blast_Score=265, Evalue=3e-71,
Organism=Saccharomyces cerevisiae, GI6321610, Length=542, Percent_Identity=23.2472324723247, Blast_Score=111, Evalue=5e-25,
Organism=Drosophila melanogaster, GI24655311, Length=599, Percent_Identity=28.5475792988314, Blast_Score=251, Evalue=1e-66,
Organism=Drosophila melanogaster, GI21358483, Length=517, Percent_Identity=24.3713733075435, Blast_Score=139, Evalue=9e-33,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080 [H]

Pfam domain/function: PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 82499; Mature: 82368

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS50886 TRBD ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPMLPPLKEQLNPEKDRGINCCAPWRDCVLRVVLSLQFGLYQEKRILSMANDPRKLLVTC
CCCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEEE
ALPYANGSIHLGHMLEHIQADIWVRYQRLRGNTVNFICADDAHGTPIMLKAQQMGMTPEA
EEECCCCCEEHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEHHHHCCCHHH
MIEMVSEEHQRDFAGFDISFDNYHSTHSDENRELASHIYLQLKKNGFISSRTISQLFDPE
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHEEEEECCCCCCHHHHHHHHCCC
KEMFLPDRFVKGTCPKCKSEDQYGDNCDACGETYSPTELINPKSAVSGATPVMKDSEHFF
HHHCCCCHHHCCCCCCCCCCCCCCCCCHHCCCCCCCHHHCCCHHHCCCCCCCCCCCCCEE
FDLPQFESMLKEWTRSGSLQSETANKMQEWFEGGLQQWDISRDAPYFGFEIPGEKDKFFY
EECHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEECCCCCCCCCCEECCCCCCCEEE
VWLDAPIGYMGSFKNLCDKRGDLDFNEYWNKDSKTELYHFIGKDIVYFHSLFWPAMLDGS
EEECCCCCHHHHHHHHHHHCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
GFRKPTNVFVHGYVTVNGAKMSKSKGTFVKASTYLNHLDPECLRYYYAAKLNNRIDDLDL
CCCCCCEEEEEEEEEEECCEECCCCCCEEEHHHHHHCCCHHHHHHHHHHHHCCCCCCCCC
NLEDFTQRVNADVVNKIVNLASRNAGFITKRFDGKLSAHFAEPELYAEFAGAADRIAELF
CHHHHHHHHCHHHHHHHHHHHCCCCCCEEEECCCCEEEECCCHHHHHHHHCHHHHHHHHH
EAREFGRAIREITALADKANQYVDEKAPWVVAKQEGQDQALQDICTVGINLFRVLMTYLK
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
PVMPALAERTEAFLNQELTWEGVATPLTDHAVTPFKALFNRIDPKQVEAMIEASKAEAAA
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHH
EKAAADAAKPKSAETELSKDPLAAEIEFDDFAKVDLRIAKILSCEAVEKSDKLLKFELDI
HHHHHHCCCCCCCCHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECC
GGETRQVFSGIKSAYQPEDLIGKYTVVVANLKPRKMKFGMSEGMILAAGPGGSDLWLLEP
CCCHHHHHHHHHHCCCCHHHCCCEEEEEECCCCHHHCCCCCCCEEEEECCCCCCEEEECC
HQGAQAGMRVM
CCCCCCCCCCC
>Mature Secondary Structure 
PMLPPLKEQLNPEKDRGINCCAPWRDCVLRVVLSLQFGLYQEKRILSMANDPRKLLVTC
CCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEEE
ALPYANGSIHLGHMLEHIQADIWVRYQRLRGNTVNFICADDAHGTPIMLKAQQMGMTPEA
EEECCCCCEEHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEHHHHCCCHHH
MIEMVSEEHQRDFAGFDISFDNYHSTHSDENRELASHIYLQLKKNGFISSRTISQLFDPE
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHEEEEECCCCCCHHHHHHHHCCC
KEMFLPDRFVKGTCPKCKSEDQYGDNCDACGETYSPTELINPKSAVSGATPVMKDSEHFF
HHHCCCCHHHCCCCCCCCCCCCCCCCCHHCCCCCCCHHHCCCHHHCCCCCCCCCCCCCEE
FDLPQFESMLKEWTRSGSLQSETANKMQEWFEGGLQQWDISRDAPYFGFEIPGEKDKFFY
EECHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEECCCCCCCCCCEECCCCCCCEEE
VWLDAPIGYMGSFKNLCDKRGDLDFNEYWNKDSKTELYHFIGKDIVYFHSLFWPAMLDGS
EEECCCCCHHHHHHHHHHHCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
GFRKPTNVFVHGYVTVNGAKMSKSKGTFVKASTYLNHLDPECLRYYYAAKLNNRIDDLDL
CCCCCCEEEEEEEEEEECCEECCCCCCEEEHHHHHHCCCHHHHHHHHHHHHCCCCCCCCC
NLEDFTQRVNADVVNKIVNLASRNAGFITKRFDGKLSAHFAEPELYAEFAGAADRIAELF
CHHHHHHHHCHHHHHHHHHHHCCCCCCEEEECCCCEEEECCCHHHHHHHHCHHHHHHHHH
EAREFGRAIREITALADKANQYVDEKAPWVVAKQEGQDQALQDICTVGINLFRVLMTYLK
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
PVMPALAERTEAFLNQELTWEGVATPLTDHAVTPFKALFNRIDPKQVEAMIEASKAEAAA
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHH
EKAAADAAKPKSAETELSKDPLAAEIEFDDFAKVDLRIAKILSCEAVEKSDKLLKFELDI
HHHHHHCCCCCCCCHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECC
GGETRQVFSGIKSAYQPEDLIGKYTVVVANLKPRKMKFGMSEGMILAAGPGGSDLWLLEP
CCCHHHHHHHHHHCCCCHHHCCCEEEEEECCCCHHHCCCCCCCEEEEECCCCCCEEEECC
HQGAQAGMRVM
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA