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The map label for this gene is cysK [H]

Identifier: 227081142

GI number: 227081142

Start: 989604

End: 990572

Strand: Reverse

Name: cysK [H]

Synonym: VCM66_0924

Alternate gene names: 227081142

Gene position: 990572-989604 (Counterclockwise)

Preceding gene: 227081143

Following gene: 227081141

Centisome position: 34.25

GC content: 46.13

Gene sequence:

>969_bases
ATGAGCAAAATCTACGAAGACAATTCACTGACTATTGGTAACACCCCACTAGTGCGCTTAAACAAAGTCTCTAACGGCAA
AGTGCTGGCTAAGATTGAAGCACGCAACCCAAGCTTCAGCGTAAAATGCCGAATCGGAGCCAATATGATTTGGGAAGCTG
AAAAGCAAGGCAAACTCAAGCCTGGCGTTGAATTAGTAGAGCCAACAAGTGGTAATACTGGTGTTGCCCTAGCGTTTGTT
GCTGCAGCTCGTGGATACAAGTTAACTCTGACCATGCCTGAATCGATGAGTTTAGAGCGTCGTAAGCTACTCAAAGCACT
TGGCGCCAATTTAGAACTGACTGAAGCAGCAAAAGGCATGAAAGGCGCGATTGCGAAAGCAGAAGAGATTGTTGCCAGCA
ACCCAGAAAAATATTTGCTCCTCCAGCAATTCAATAACCCAGCTAACCCGCAGATCCATGAAAAGACCACAGGCCCAGAG
ATTTGGGATGCAACAGATGGTCAAATTGATGTGTTTGTTGCTGGTGTAGGTACTGGCGGTACATTAACGGGTACCAGTCG
CTACATCAAAGGCACTCAAGGTAAAGCTATTCTTTCTGTTGCGGTTGAACCGGCTGAATCATCGGTCATTACACAAGCGC
TGGCGGGAGAAGAAATCAAACCTGCACCACATAAAATTCAAGGTATCGGTGCCGGTTTTATCCCTGGTAACTTAGATTTA
AGCTTGATTGATCGTGTTGAGCGAGTCACCTCAGACGAGGCGATTGAAATGGCGCGTCGCCTAATGGAAGAAGAAGGCAT
TCTCGCGGGGATCTCTTCTGGTGCTGCTGTTGTTGCCGCTAACCGAATCGCACAATTACCTGAATTTGAAGGAAAAACTA
TAGTTACCGTGTTACCAAGTTCAGGTGAACGTTACTTGAGCACAGCTCTATTCGCAGGCATTTTCACTGATAAAGAAAAC
CAGCAGTAA

Upstream 100 bases:

>100_bases
TTTTTAATCCTTTTCGCTTAGTGAATATGAACTTATTCTCTGTCCATATTCACTAAGTATTCACGCACACTCACTGCGTA
ACGAATGAAGGAACATCGCC

Downstream 100 bases:

>100_bases
TACGTGGTTAAATCAAATTTTCGTTTAAAAAAGCCCCAGTCAGGGGCTTTTTTGTTGATCCCTGCCTCACCTTTGGTAAT
ATCGGCATGTTTTATTTTTA

Product: cysteine synthase A

Products: NA

Alternate protein names: CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine sulfhydrylase A; Sulfate starvation-induced protein 5; SSI5 [H]

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MSKIYEDNSLTIGNTPLVRLNKVSNGKVLAKIEARNPSFSVKCRIGANMIWEAEKQGKLKPGVELVEPTSGNTGVALAFV
AAARGYKLTLTMPESMSLERRKLLKALGANLELTEAAKGMKGAIAKAEEIVASNPEKYLLLQQFNNPANPQIHEKTTGPE
IWDATDGQIDVFVAGVGTGGTLTGTSRYIKGTQGKAILSVAVEPAESSVITQALAGEEIKPAPHKIQGIGAGFIPGNLDL
SLIDRVERVTSDEAIEMARRLMEEEGILAGISSGAAVVAANRIAQLPEFEGKTIVTVLPSSGERYLSTALFAGIFTDKEN
QQ

Sequences:

>Translated_322_residues
MSKIYEDNSLTIGNTPLVRLNKVSNGKVLAKIEARNPSFSVKCRIGANMIWEAEKQGKLKPGVELVEPTSGNTGVALAFV
AAARGYKLTLTMPESMSLERRKLLKALGANLELTEAAKGMKGAIAKAEEIVASNPEKYLLLQQFNNPANPQIHEKTTGPE
IWDATDGQIDVFVAGVGTGGTLTGTSRYIKGTQGKAILSVAVEPAESSVITQALAGEEIKPAPHKIQGIGAGFIPGNLDL
SLIDRVERVTSDEAIEMARRLMEEEGILAGISSGAAVVAANRIAQLPEFEGKTIVTVLPSSGERYLSTALFAGIFTDKEN
QQ
>Mature_321_residues
SKIYEDNSLTIGNTPLVRLNKVSNGKVLAKIEARNPSFSVKCRIGANMIWEAEKQGKLKPGVELVEPTSGNTGVALAFVA
AARGYKLTLTMPESMSLERRKLLKALGANLELTEAAKGMKGAIAKAEEIVASNPEKYLLLQQFNNPANPQIHEKTTGPEI
WDATDGQIDVFVAGVGTGGTLTGTSRYIKGTQGKAILSVAVEPAESSVITQALAGEEIKPAPHKIQGIGAGFIPGNLDLS
LIDRVERVTSDEAIEMARRLMEEEGILAGISSGAAVVAANRIAQLPEFEGKTIVTVLPSSGERYLSTALFAGIFTDKENQ
Q

Specific function: Cysteine biosynthesis. [C]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=316, Percent_Identity=37.9746835443038, Blast_Score=196, Evalue=3e-50,
Organism=Homo sapiens, GI295821200, Length=316, Percent_Identity=37.9746835443038, Blast_Score=196, Evalue=3e-50,
Organism=Homo sapiens, GI4557415, Length=316, Percent_Identity=37.9746835443038, Blast_Score=196, Evalue=3e-50,
Organism=Escherichia coli, GI1788754, Length=323, Percent_Identity=79.8761609907121, Blast_Score=507, Evalue=1e-145,
Organism=Escherichia coli, GI2367138, Length=305, Percent_Identity=43.9344262295082, Blast_Score=221, Evalue=4e-59,
Organism=Caenorhabditis elegans, GI17535051, Length=315, Percent_Identity=51.7460317460318, Blast_Score=298, Evalue=3e-81,
Organism=Caenorhabditis elegans, GI17562970, Length=318, Percent_Identity=49.3710691823899, Blast_Score=289, Evalue=2e-78,
Organism=Caenorhabditis elegans, GI115535073, Length=317, Percent_Identity=53.9432176656151, Blast_Score=287, Evalue=5e-78,
Organism=Caenorhabditis elegans, GI32566674, Length=307, Percent_Identity=50.814332247557, Blast_Score=283, Evalue=1e-76,
Organism=Caenorhabditis elegans, GI17561720, Length=316, Percent_Identity=49.6835443037975, Blast_Score=264, Evalue=5e-71,
Organism=Caenorhabditis elegans, GI32566672, Length=226, Percent_Identity=49.1150442477876, Blast_Score=212, Evalue=2e-55,
Organism=Caenorhabditis elegans, GI17534315, Length=319, Percent_Identity=40.7523510971787, Blast_Score=155, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI25147552, Length=313, Percent_Identity=38.3386581469649, Blast_Score=143, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17561716, Length=131, Percent_Identity=50.381679389313, Blast_Score=111, Evalue=6e-25,
Organism=Saccharomyces cerevisiae, GI6321594, Length=313, Percent_Identity=36.741214057508, Blast_Score=172, Evalue=8e-44,
Organism=Saccharomyces cerevisiae, GI6321449, Length=323, Percent_Identity=34.984520123839, Blast_Score=135, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24643623, Length=304, Percent_Identity=37.828947368421, Blast_Score=159, Evalue=2e-39,
Organism=Drosophila melanogaster, GI20129101, Length=304, Percent_Identity=37.828947368421, Blast_Score=159, Evalue=2e-39,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR005856
- InterPro:   IPR005859
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =2.5.1.47 [H]

Molecular weight: Translated: 34229; Mature: 34097

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS00901 CYS_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKIYEDNSLTIGNTPLVRLNKVSNGKVLAKIEARNPSFSVKCRIGANMIWEAEKQGKLK
CCCEECCCCEEECCCCEEEEEECCCCEEEEEEECCCCCEEEEEEECCCEEEECCCCCCCC
PGVELVEPTSGNTGVALAFVAAARGYKLTLTMPESMSLERRKLLKALGANLELTEAAKGM
CCCEEECCCCCCCCEEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHCCCCEEHHHHCCH
KGAIAKAEEIVASNPEKYLLLQQFNNPANPQIHEKTTGPEIWDATDGQIDVFVAGVGTGG
HHHHHHHHHHHHCCCCCEEEEEECCCCCCCCEECCCCCCCCCCCCCCEEEEEEEEECCCC
TLTGTSRYIKGTQGKAILSVAVEPAESSVITQALAGEEIKPAPHKIQGIGAGFIPGNLDL
EECCCCCEEECCCCCEEEEEEECCCHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCH
SLIDRVERVTSDEAIEMARRLMEEEGILAGISSGAAVVAANRIAQLPEFEGKTIVTVLPS
HHHHHHHHHCCHHHHHHHHHHHHHCCCEEECCCCCEEEEHHHHHCCCCCCCCEEEEEECC
SGERYLSTALFAGIFTDKENQQ
CCCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SKIYEDNSLTIGNTPLVRLNKVSNGKVLAKIEARNPSFSVKCRIGANMIWEAEKQGKLK
CCEECCCCEEECCCCEEEEEECCCCEEEEEEECCCCCEEEEEEECCCEEEECCCCCCCC
PGVELVEPTSGNTGVALAFVAAARGYKLTLTMPESMSLERRKLLKALGANLELTEAAKGM
CCCEEECCCCCCCCEEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHCCCCEEHHHHCCH
KGAIAKAEEIVASNPEKYLLLQQFNNPANPQIHEKTTGPEIWDATDGQIDVFVAGVGTGG
HHHHHHHHHHHHCCCCCEEEEEECCCCCCCCEECCCCCCCCCCCCCCEEEEEEEEECCCC
TLTGTSRYIKGTQGKAILSVAVEPAESSVITQALAGEEIKPAPHKIQGIGAGFIPGNLDL
EECCCCCEEECCCCCEEEEEEECCCHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCH
SLIDRVERVTSDEAIEMARRLMEEEGILAGISSGAAVVAANRIAQLPEFEGKTIVTVLPS
HHHHHHHHHCCHHHHHHHHHHHHHCCCEEECCCCCEEEEHHHHHCCCCCCCCEEEEEECC
SGERYLSTALFAGIFTDKENQQ
CCCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]