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The map label for this gene is ubiF [H]

Identifier: 227081137

GI number: 227081137

Start: 985258

End: 986451

Strand: Direct

Name: ubiF [H]

Synonym: VCM66_0919

Alternate gene names: 227081137

Gene position: 985258-986451 (Clockwise)

Preceding gene: 227081131

Following gene: 227081144

Centisome position: 34.06

GC content: 50.25

Gene sequence:

>1194_bases
GTGAAGAGAAAATACTGGGTGGGAAAAGGTATGAGTGGCAACAAATTTGACATCCTCGTGGTGGGGGGCGGCATGGTGGG
CGCGGCTGCTGCACTGGGCTTTGCACGCCAAGGACGCAAGGTTGCGGTAATTGATTCTGGCGCGCCGCACGCTTTTGAGC
CGAGTCAGCCTATGGATGTGCGTGTCTCTGCGATTTCGCAAACTTCAGTAGGACTGCTAGAAGAACTGGGTGCTTGGTCA
AGTGTTGTTGGTATGCGTGCTTGTCCTTATCGTCACTTAGAAACGTGGGAACACCCAGAATGTCGAACGCGTTTTTCTTG
TGAGTCGCTCGGGTTGCCACAATTAGGTTACATGGTAGAAAACCGAGTCATTCAATTAGCACTATGGCAGCAATTTGCAG
CTTATCCGCAGTTGACTTTAATGTGTCCCGAACAATTGGCGCAGTTGAATGTGAGTGCAGAGGGATGCCGTGTTACCTTG
CAATCGGGCGCTGAAATTGAGTGTGATTGGGTAATCGGTGCGGATGGCGCAAATTCCCAGGTGCGCAATCTGGTGCGTAT
CGGCGTAACGGCGTGGGATTATCGTCAACACTGTATGTTGATTAATGTGCAGACGGAGCAGATCCAACAAGAAACCACGT
GGCAGCAATTTTTTCCAACAGGTCCGCGCTCCTACTTACCTTTGGGCGGCAATCAGGCGTCTTTGGTGTGGTATGACACA
CCGCAGCGTATTCGCAAGCTCAGCGGTCTTAATCCGGAGCAATTGCGTAGCGAAATTCTGCAGTATTTCCCGGCCGAGTT
GGGGAATATTAAAGTGTTGCAACACGGTGCTTTCCCATTAACGCGCCGTCATGCTCAGCAGTATGTGAAAAATCGCTGTA
TTTTGATTGGGGATGCAGCACACACCATAAACCCATTAGCAGGGCAGGGCGTCAATTTGGGCTTTAAAGATGTGGCCGTG
TTGCTGGCGCAAACTCAAGAGCAGGCGTTAAATCAGGCATCTTTTGAACGCTATGAACGCTGCCGTCGTCCCGATAATTT
ACTGATGCAAACAGGGATGGACTTGTTCTACAAAACCTTCAGTAACTCAATCACACCAGTGAAGTTTCTGCGTAATGCTG
CGTTGGCATTGGCTGAGCGTTCTGGCCCACTGAAAACGCAAGTGCTGCGTTATGCCCTTGGTTTTACGAATTGA

Upstream 100 bases:

>100_bases
ATTCTCTTGTAACCCTTGTTGTGATTGGCTTTTTACCTTATTACGAGCAAGGTTTTAACGTAAAAAATGTCGCAATATTT
CCCTTACTCCGTACAATGCC

Downstream 100 bases:

>100_bases
ATGTTGTTTGAGATGAGCGCTAGTTAGGCGCTATTGGCTATGTCAAAAAACTCGGCCGTAAAAACAAAAAACGCTGCACT
CGGCAGCGTTTTTTACATCG

Product: 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MKRKYWVGKGMSGNKFDILVVGGGMVGAAAALGFARQGRKVAVIDSGAPHAFEPSQPMDVRVSAISQTSVGLLEELGAWS
SVVGMRACPYRHLETWEHPECRTRFSCESLGLPQLGYMVENRVIQLALWQQFAAYPQLTLMCPEQLAQLNVSAEGCRVTL
QSGAEIECDWVIGADGANSQVRNLVRIGVTAWDYRQHCMLINVQTEQIQQETTWQQFFPTGPRSYLPLGGNQASLVWYDT
PQRIRKLSGLNPEQLRSEILQYFPAELGNIKVLQHGAFPLTRRHAQQYVKNRCILIGDAAHTINPLAGQGVNLGFKDVAV
LLAQTQEQALNQASFERYERCRRPDNLLMQTGMDLFYKTFSNSITPVKFLRNAALALAERSGPLKTQVLRYALGFTN

Sequences:

>Translated_397_residues
MKRKYWVGKGMSGNKFDILVVGGGMVGAAAALGFARQGRKVAVIDSGAPHAFEPSQPMDVRVSAISQTSVGLLEELGAWS
SVVGMRACPYRHLETWEHPECRTRFSCESLGLPQLGYMVENRVIQLALWQQFAAYPQLTLMCPEQLAQLNVSAEGCRVTL
QSGAEIECDWVIGADGANSQVRNLVRIGVTAWDYRQHCMLINVQTEQIQQETTWQQFFPTGPRSYLPLGGNQASLVWYDT
PQRIRKLSGLNPEQLRSEILQYFPAELGNIKVLQHGAFPLTRRHAQQYVKNRCILIGDAAHTINPLAGQGVNLGFKDVAV
LLAQTQEQALNQASFERYERCRRPDNLLMQTGMDLFYKTFSNSITPVKFLRNAALALAERSGPLKTQVLRYALGFTN
>Mature_397_residues
MKRKYWVGKGMSGNKFDILVVGGGMVGAAAALGFARQGRKVAVIDSGAPHAFEPSQPMDVRVSAISQTSVGLLEELGAWS
SVVGMRACPYRHLETWEHPECRTRFSCESLGLPQLGYMVENRVIQLALWQQFAAYPQLTLMCPEQLAQLNVSAEGCRVTL
QSGAEIECDWVIGADGANSQVRNLVRIGVTAWDYRQHCMLINVQTEQIQQETTWQQFFPTGPRSYLPLGGNQASLVWYDT
PQRIRKLSGLNPEQLRSEILQYFPAELGNIKVLQHGAFPLTRRHAQQYVKNRCILIGDAAHTINPLAGQGVNLGFKDVAV
LLAQTQEQALNQASFERYERCRRPDNLLMQTGMDLFYKTFSNSITPVKFLRNAALALAERSGPLKTQVLRYALGFTN

Specific function: Oxygenase that introduces the hydroxyl group at carbon five of 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol resulting in the formation of 2-octaprenyl-3-methyl-5-hydroxy-6-methoxy-1,4- benzoquinol [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ubiH/COQ6 family [H]

Homologues:

Organism=Homo sapiens, GI32967305, Length=421, Percent_Identity=29.6912114014252, Blast_Score=178, Evalue=7e-45,
Organism=Homo sapiens, GI32967307, Length=335, Percent_Identity=29.8507462686567, Blast_Score=149, Evalue=4e-36,
Organism=Escherichia coli, GI1786883, Length=391, Percent_Identity=51.9181585677749, Blast_Score=389, Evalue=1e-109,
Organism=Escherichia coli, GI1789273, Length=390, Percent_Identity=36.6666666666667, Blast_Score=241, Evalue=7e-65,
Organism=Escherichia coli, GI1789274, Length=392, Percent_Identity=28.0612244897959, Blast_Score=142, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI25146631, Length=431, Percent_Identity=27.3781902552204, Blast_Score=170, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6321694, Length=448, Percent_Identity=24.7767857142857, Blast_Score=113, Evalue=5e-26,
Organism=Drosophila melanogaster, GI19920760, Length=437, Percent_Identity=28.1464530892449, Blast_Score=162, Evalue=3e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042
- InterPro:   IPR018168
- InterPro:   IPR010971 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.-

Molecular weight: Translated: 44263; Mature: 44263

Theoretical pI: Translated: 8.76; Mature: 8.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRKYWVGKGMSGNKFDILVVGGGMVGAAAALGFARQGRKVAVIDSGAPHAFEPSQPMDV
CCCCEEECCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCEE
RVSAISQTSVGLLEELGAWSSVVGMRACPYRHLETWEHPECRTRFSCESLGLPQLGYMVE
EEEEHHHHHHHHHHHHCCHHHHHHHHCCCCHHCCCCCCCCHHCCCCHHHCCCCHHHHHHH
NRVIQLALWQQFAAYPQLTLMCPEQLAQLNVSAEGCRVTLQSGAEIECDWVIGADGANSQ
HHHHHHHHHHHHHCCCCEEEECHHHHHHCCCCCCCCEEEECCCCEEEEEEEEECCCCCHH
VRNLVRIGVTAWDYRQHCMLINVQTEQIQQETTWQQFFPTGPRSYLPLGGNQASLVWYDT
HHHHHHHCCEEECCCCCEEEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECC
PQRIRKLSGLNPEQLRSEILQYFPAELGNIKVLQHGAFPLTRRHAQQYVKNRCILIGDAA
HHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHCCEEEEECCH
HTINPLAGQGVNLGFKDVAVLLAQTQEQALNQASFERYERCRRPDNLLMQTGMDLFYKTF
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
SNSITPVKFLRNAALALAERSGPLKTQVLRYALGFTN
CCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MKRKYWVGKGMSGNKFDILVVGGGMVGAAAALGFARQGRKVAVIDSGAPHAFEPSQPMDV
CCCCEEECCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCEE
RVSAISQTSVGLLEELGAWSSVVGMRACPYRHLETWEHPECRTRFSCESLGLPQLGYMVE
EEEEHHHHHHHHHHHHCCHHHHHHHHCCCCHHCCCCCCCCHHCCCCHHHCCCCHHHHHHH
NRVIQLALWQQFAAYPQLTLMCPEQLAQLNVSAEGCRVTLQSGAEIECDWVIGADGANSQ
HHHHHHHHHHHHHCCCCEEEECHHHHHHCCCCCCCCEEEECCCCEEEEEEEEECCCCCHH
VRNLVRIGVTAWDYRQHCMLINVQTEQIQQETTWQQFFPTGPRSYLPLGGNQASLVWYDT
HHHHHHHCCEEECCCCCEEEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECC
PQRIRKLSGLNPEQLRSEILQYFPAELGNIKVLQHGAFPLTRRHAQQYVKNRCILIGDAA
HHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHCCEEEEECCH
HTINPLAGQGVNLGFKDVAVLLAQTQEQALNQASFERYERCRRPDNLLMQTGMDLFYKTF
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
SNSITPVKFLRNAALALAERSGPLKTQVLRYALGFTN
CCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydroxylation [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10802164 [H]