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The map label for this gene is proS

Identifier: 227081051

GI number: 227081051

Start: 895306

End: 897021

Strand: Reverse

Name: proS

Synonym: VCM66_0832

Alternate gene names: 227081051

Gene position: 897021-895306 (Counterclockwise)

Preceding gene: 227081052

Following gene: 227081049

Centisome position: 31.01

GC content: 50.99

Gene sequence:

>1716_bases
ATGCGTACCAGTAACTATCTTCTTTCCACTCTGAAGGAGACTCCAAACGACGCAGAAGTTGTGAGCCATAAGCTCATGCT
CCGTGCAGGTATGATCCGTAAGCTGGCTTCAGGTCTCTATACTTGGCTGCCTACTGGTCTGCGTGTACTGCGTAAAGTCG
AAAACATCGTTCGCCAAGAGATCGATAATGCCGGTGCAATCGAAACCTTGATGCCGGTGGTGCAGCCGTTTGAATTGTGG
GAAGAGACTGGTCGCTCTGAAAAAATGGGCCCAGAGCTACTGCGCTTTACTGACCGCCATGAGCGTCCGTTTGTTTTAAG
CCCAACTGCGGAAGAAGTGATCACGGCGCTGGTGCGTAATGAGGTGAGCTCTTACAAGCAGTTGCCACTGAACTTGTATC
AAATTCAAACCAAATTCCGTGATGAACGCCGTCCACGTTTTGGTGTGATGCGTGCCCGTGAATTCTGCATGATGGATGCG
TACAGCTTTGATATCGACAAAGCGGGCCTAGAAAAATCCTACGAAGCCATGCACGTTGCGTATTGCAAAGCGTTTGACCG
TATGGGCCTCGATTACCGCCCAGTGCTAGCGGATACTGGTGCTATCGGTGGTAACGGCTCACACGAATTCCACGTACTGG
CAGAAAGTGGCGAAGATCTGATCGCCTTCTCAACCGAATCGGATTACGCAGCCAACATCGAAAAAGCCGAAGCGGTTGCA
CCGGCGATTGAGCGCCCAGCGCCTACGCAAGCGATGACCTTGGTTGATACCCCTAACGCAAAAACCATCGCAGAATTGGT
TGAGCAGCACGGTCTGCCTATCGAAAAAACGGTGAAAACCTTATTTGTCAAAGCCTCAGATGAAATCGATGCGCCTATCG
TTGCGCTGATCATCCGTGGTGACCATGAACTGAACGAAATCAAAGCCGAGAAACTGGCCGAAGTGGCCTCGCCGCTGGAA
ATGGCCAGCGAAGAAGAGATTCGCGCGCTGATCGGTGCAGGCCCTGGCTCATTAGGCCCTGTCGGCCTGAAACTGCCATT
TATTGTTGACCGCACCGTTGCTGTGATGAACGATTTCGGCGCTGGCGCAAACATCGATGGTAAACACTACTTCGGTATCA
ACTGGGGTCGTGATGTTGAACTGGGTAAAGTCGAAGATCTGCGTAATGTGGTTGAAGGCGATCCAAGCCCATGTGGCAAA
GGCACACTGATGCTCAAGCGCGGTATCGAAGTGGGTCACATCTTCCAATTGGGTACCAACTACTCTGAAAAGATGAACTG
TGGCGTACTCGATTCGAACGGTAAAAACGTCATTCTTGAGATGGGTTGCTACGGTATCGGCGTCTCTCGCGTCGTCGCGG
CAGCCATCGAACAAAACCATGACGATTACGGCATCATTTGGCCTGATGCGATTGCACCTTTCCAAGTGGCTATTGTGCCG
ATGAACATGCACAAATCTGAGCGTGTTCAGCAAGCCGCTGAAAAATTGTATGCAGAATTGACAGCTGCAGGCATCGAAGT
GCTGTTTGATGATCGTAAAGAGCGTCCGGGCGTCATGTTCTCAGATATGGAGCTGATCGGCGTGCCACACACCATCATCA
TTGGTGATCGCAGCATGGATGAAGGCCACTTCGAATACAAAAATCGCCGTCAAGGTGAGAAAGAAGCCGTGGCAATGGAG
TCAATCATCGACTTTATCCAAGCCAAACTGGCCTAA

Upstream 100 bases:

>100_bases
CACCCACTTAGCCAACACGCCTGCCGCTTGCAGTAAGAAGGGAATGACGTCACTCCTGTTAAAGCGATCCTTTAAACTTT
GATGAAACGGATACCATAGA

Downstream 100 bases:

>100_bases
TTTTCGGTCACATTCACTTAAAAAGGCTGCCATCGAGCAGCCTTTTTATTACCAGTTTTCCCCAAACGACTTGGATTTGC
AGGTAGGCGGCAAGTGAGTT

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 571; Mature: 571

Protein sequence:

>571_residues
MRTSNYLLSTLKETPNDAEVVSHKLMLRAGMIRKLASGLYTWLPTGLRVLRKVENIVRQEIDNAGAIETLMPVVQPFELW
EETGRSEKMGPELLRFTDRHERPFVLSPTAEEVITALVRNEVSSYKQLPLNLYQIQTKFRDERRPRFGVMRAREFCMMDA
YSFDIDKAGLEKSYEAMHVAYCKAFDRMGLDYRPVLADTGAIGGNGSHEFHVLAESGEDLIAFSTESDYAANIEKAEAVA
PAIERPAPTQAMTLVDTPNAKTIAELVEQHGLPIEKTVKTLFVKASDEIDAPIVALIIRGDHELNEIKAEKLAEVASPLE
MASEEEIRALIGAGPGSLGPVGLKLPFIVDRTVAVMNDFGAGANIDGKHYFGINWGRDVELGKVEDLRNVVEGDPSPCGK
GTLMLKRGIEVGHIFQLGTNYSEKMNCGVLDSNGKNVILEMGCYGIGVSRVVAAAIEQNHDDYGIIWPDAIAPFQVAIVP
MNMHKSERVQQAAEKLYAELTAAGIEVLFDDRKERPGVMFSDMELIGVPHTIIIGDRSMDEGHFEYKNRRQGEKEAVAME
SIIDFIQAKLA

Sequences:

>Translated_571_residues
MRTSNYLLSTLKETPNDAEVVSHKLMLRAGMIRKLASGLYTWLPTGLRVLRKVENIVRQEIDNAGAIETLMPVVQPFELW
EETGRSEKMGPELLRFTDRHERPFVLSPTAEEVITALVRNEVSSYKQLPLNLYQIQTKFRDERRPRFGVMRAREFCMMDA
YSFDIDKAGLEKSYEAMHVAYCKAFDRMGLDYRPVLADTGAIGGNGSHEFHVLAESGEDLIAFSTESDYAANIEKAEAVA
PAIERPAPTQAMTLVDTPNAKTIAELVEQHGLPIEKTVKTLFVKASDEIDAPIVALIIRGDHELNEIKAEKLAEVASPLE
MASEEEIRALIGAGPGSLGPVGLKLPFIVDRTVAVMNDFGAGANIDGKHYFGINWGRDVELGKVEDLRNVVEGDPSPCGK
GTLMLKRGIEVGHIFQLGTNYSEKMNCGVLDSNGKNVILEMGCYGIGVSRVVAAAIEQNHDDYGIIWPDAIAPFQVAIVP
MNMHKSERVQQAAEKLYAELTAAGIEVLFDDRKERPGVMFSDMELIGVPHTIIIGDRSMDEGHFEYKNRRQGEKEAVAME
SIIDFIQAKLA
>Mature_571_residues
MRTSNYLLSTLKETPNDAEVVSHKLMLRAGMIRKLASGLYTWLPTGLRVLRKVENIVRQEIDNAGAIETLMPVVQPFELW
EETGRSEKMGPELLRFTDRHERPFVLSPTAEEVITALVRNEVSSYKQLPLNLYQIQTKFRDERRPRFGVMRAREFCMMDA
YSFDIDKAGLEKSYEAMHVAYCKAFDRMGLDYRPVLADTGAIGGNGSHEFHVLAESGEDLIAFSTESDYAANIEKAEAVA
PAIERPAPTQAMTLVDTPNAKTIAELVEQHGLPIEKTVKTLFVKASDEIDAPIVALIIRGDHELNEIKAEKLAEVASPLE
MASEEEIRALIGAGPGSLGPVGLKLPFIVDRTVAVMNDFGAGANIDGKHYFGINWGRDVELGKVEDLRNVVEGDPSPCGK
GTLMLKRGIEVGHIFQLGTNYSEKMNCGVLDSNGKNVILEMGCYGIGVSRVVAAAIEQNHDDYGIIWPDAIAPFQVAIVP
MNMHKSERVQQAAEKLYAELTAAGIEVLFDDRKERPGVMFSDMELIGVPHTIIIGDRSMDEGHFEYKNRRQGEKEAVAME
SIIDFIQAKLA

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=241, Percent_Identity=41.0788381742739, Blast_Score=199, Evalue=6e-51,
Organism=Escherichia coli, GI1786392, Length=566, Percent_Identity=71.9081272084806, Blast_Score=856, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115532348, Length=252, Percent_Identity=38.0952380952381, Blast_Score=170, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI193203271, Length=93, Percent_Identity=38.7096774193548, Blast_Score=79, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6320931, Length=202, Percent_Identity=39.1089108910891, Blast_Score=160, Evalue=6e-40,
Organism=Drosophila melanogaster, GI24656200, Length=225, Percent_Identity=36.4444444444444, Blast_Score=158, Evalue=1e-38,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_VIBC3 (A5F340)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F340
- SMR:   A5F340
- STRING:   A5F340
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A0400
- eggNOG:   COG0442
- HOGENOM:   HBG403504
- OMA:   DFVLGPT
- ProtClustDB:   PRK09194
- BioCyc:   VCHO345073:VC0395_A0400-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01569
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.90.960.10
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK; SSF52954 Anticodon_bd; SSF55826 YbaK/aa-tRNA-synth-assoc-reg

EC number: =6.1.1.15

Molecular weight: Translated: 63252; Mature: 63252

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTSNYLLSTLKETPNDAEVVSHKLMLRAGMIRKLASGLYTWLPTGLRVLRKVENIVRQE
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IDNAGAIETLMPVVQPFELWEETGRSEKMGPELLRFTDRHERPFVLSPTAEEVITALVRN
HCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCEEECCCHHHHHHHHHHH
EVSSYKQLPLNLYQIQTKFRDERRPRFGVMRAREFCMMDAYSFDIDKAGLEKSYEAMHVA
HHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
YCKAFDRMGLDYRPVLADTGAIGGNGSHEFHVLAESGEDLIAFSTESDYAANIEKAEAVA
HHHHHHHCCCCCCCHHCCCCCCCCCCCCEEEEEECCCCCEEEEECCCCCCCCCHHHHHHH
PAIERPAPTQAMTLVDTPNAKTIAELVEQHGLPIEKTVKTLFVKASDEIDAPIVALIIRG
HHHCCCCCCCEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHEEECCCCCCCCEEEEEEEC
DHELNEIKAEKLAEVASPLEMASEEEIRALIGAGPGSLGPVGLKLPFIVDRTVAVMNDFG
CCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCEECCHHHHHHHHHHHCCC
AGANIDGKHYFGINWGRDVELGKVEDLRNVVEGDPSPCGKGTLMLKRGIEVGHIFQLGTN
CCCCCCCCEEECCCCCCCCCCCCHHHHHHHHCCCCCCCCCCEEEEECCCCEEEEEEECCC
YSEKMNCGVLDSNGKNVILEMGCYGIGVSRVVAAAIEQNHDDYGIIWPDAIAPFQVAIVP
CCCCCCCCEEECCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCEEECCCCCCCEEEEEEE
MNMHKSERVQQAAEKLYAELTAAGIEVLFDDRKERPGVMFSDMELIGVPHTIIIGDRSMD
ECCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCEEECCCEECCCEEEEECCCCCC
EGHFEYKNRRQGEKEAVAMESIIDFIQAKLA
CCCHHHHHHCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRTSNYLLSTLKETPNDAEVVSHKLMLRAGMIRKLASGLYTWLPTGLRVLRKVENIVRQE
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IDNAGAIETLMPVVQPFELWEETGRSEKMGPELLRFTDRHERPFVLSPTAEEVITALVRN
HCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCEEECCCHHHHHHHHHHH
EVSSYKQLPLNLYQIQTKFRDERRPRFGVMRAREFCMMDAYSFDIDKAGLEKSYEAMHVA
HHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
YCKAFDRMGLDYRPVLADTGAIGGNGSHEFHVLAESGEDLIAFSTESDYAANIEKAEAVA
HHHHHHHCCCCCCCHHCCCCCCCCCCCCEEEEEECCCCCEEEEECCCCCCCCCHHHHHHH
PAIERPAPTQAMTLVDTPNAKTIAELVEQHGLPIEKTVKTLFVKASDEIDAPIVALIIRG
HHHCCCCCCCEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHEEECCCCCCCCEEEEEEEC
DHELNEIKAEKLAEVASPLEMASEEEIRALIGAGPGSLGPVGLKLPFIVDRTVAVMNDFG
CCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCEECCHHHHHHHHHHHCCC
AGANIDGKHYFGINWGRDVELGKVEDLRNVVEGDPSPCGKGTLMLKRGIEVGHIFQLGTN
CCCCCCCCEEECCCCCCCCCCCCHHHHHHHHCCCCCCCCCCEEEEECCCCEEEEEEECCC
YSEKMNCGVLDSNGKNVILEMGCYGIGVSRVVAAAIEQNHDDYGIIWPDAIAPFQVAIVP
CCCCCCCCEEECCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCEEECCCCCCCEEEEEEE
MNMHKSERVQQAAEKLYAELTAAGIEVLFDDRKERPGVMFSDMELIGVPHTIIIGDRSMD
ECCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCEEECCCEECCCEEEEECCCCCC
EGHFEYKNRRQGEKEAVAMESIIDFIQAKLA
CCCHHHHHHCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA