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The map label for this gene is tcpE

Identifier: 227081013

GI number: 227081013

Start: 855093

End: 856115

Strand: Direct

Name: tcpE

Synonym: VCM66_0793

Alternate gene names: 227081013

Gene position: 855093-856115 (Clockwise)

Preceding gene: 227081012

Following gene: 227081014

Centisome position: 29.56

GC content: 35.58

Gene sequence:

>1023_bases
ATGAAAATTATCTCCAAGAAGTATAGGCTAGAACTTTATTCAATGCTTGTGGACTTATTGAATGATAATATCCCACTCTA
TGATGCATTAAATAAAATACAAAATGAGGGAGTGGGCATCTATGATAAGAATTTTATTAAATCCATTGAATTAATTAAGG
ATAGGATGAAAAGTAATTCATCACTAACGGATGCCTTAACTGGACTAATACCAGATAAAGAAGTTTTGATGATTAATGTT
GCAGAAAACTCAGGTAAGATTTCGAGTGGTATTGCTGCAATACGCAAAAACATTATCGATGCAGATGAGATCAAATCTAA
AGCTATCTCATCTATGATCACGCCTAGCGTGATGCTTATCGTGACTATGGTTGTTATTGCTGGCTATTCAGTAAAAGTAT
TTCCCACATTTGAGTCTGTACTTCCTGTGAGCCGGTGGCCCGGTGTTACCCAAGCACTTTATAACCTTGGATTTTCATTG
TATGAAGGCTTGTGGATCAAAGTTCTTATTTTTGTCGCTATCTTCATTACGATTTTAGTTTTTATGTCTAAAAACATTAC
AGGGAATTTCAGAGATGGTTTTTTAGATAAGCTGCCACCTTTTAATTTTGTAAAGCATATTGCAGCAACAGAATTTTTGG
CCAATATGTCCATGTTGCTAGATAGTCGAGTCCCATTTAAAGAAGGTCTGGACATTGTCGATCATAAAACGACACGCTGG
CTCTCCTCACATTTGCAAAGAATGAAAGCTAACATGCAAGAAGGGCTAGACTACAAACAGGCTCTCGACACCAATTTACT
AGATAAAAAAATGCTCCTGACAATGGCTGTTTATTCAGAACTACCTAATTTTTCGGATGTAATGCAAAAATTAGCTATTG
AAGCCAATATAAATCTACATAAAAAAATTGCCACTTTAGCAGGGGTAATGAAAAATATTTCACTAATAACGTTGGCGTTG
TCTGTGATTTGGATCTTCGGTGCGATCTTTTCGCTCGTTGATAAACTAAGTTCATCTTTATAG

Upstream 100 bases:

>100_bases
AAAGATAAAGCTTATTACAATGTTTTAAAAGGTAAAGTGTGTCACGAATATTTTATGCTGCATTTCGGTCAGTTAGATCA
TGAGGATGAAAATATAATAT

Downstream 100 bases:

>100_bases
TGAGGATATATGAGATATAAAAAAACCTTAATGTTATCAATCATGATTACATCATTTAACTCATTTGCATTTAATGATAA
TTATAGTTCAACCAGTACGG

Product: toxin co-regulated pilus biosynthesis protein E

Products: NA

Alternate protein names: TCP pilus biosynthesis protein tcpE

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MKIISKKYRLELYSMLVDLLNDNIPLYDALNKIQNEGVGIYDKNFIKSIELIKDRMKSNSSLTDALTGLIPDKEVLMINV
AENSGKISSGIAAIRKNIIDADEIKSKAISSMITPSVMLIVTMVVIAGYSVKVFPTFESVLPVSRWPGVTQALYNLGFSL
YEGLWIKVLIFVAIFITILVFMSKNITGNFRDGFLDKLPPFNFVKHIAATEFLANMSMLLDSRVPFKEGLDIVDHKTTRW
LSSHLQRMKANMQEGLDYKQALDTNLLDKKMLLTMAVYSELPNFSDVMQKLAIEANINLHKKIATLAGVMKNISLITLAL
SVIWIFGAIFSLVDKLSSSL

Sequences:

>Translated_340_residues
MKIISKKYRLELYSMLVDLLNDNIPLYDALNKIQNEGVGIYDKNFIKSIELIKDRMKSNSSLTDALTGLIPDKEVLMINV
AENSGKISSGIAAIRKNIIDADEIKSKAISSMITPSVMLIVTMVVIAGYSVKVFPTFESVLPVSRWPGVTQALYNLGFSL
YEGLWIKVLIFVAIFITILVFMSKNITGNFRDGFLDKLPPFNFVKHIAATEFLANMSMLLDSRVPFKEGLDIVDHKTTRW
LSSHLQRMKANMQEGLDYKQALDTNLLDKKMLLTMAVYSELPNFSDVMQKLAIEANINLHKKIATLAGVMKNISLITLAL
SVIWIFGAIFSLVDKLSSSL
>Mature_340_residues
MKIISKKYRLELYSMLVDLLNDNIPLYDALNKIQNEGVGIYDKNFIKSIELIKDRMKSNSSLTDALTGLIPDKEVLMINV
AENSGKISSGIAAIRKNIIDADEIKSKAISSMITPSVMLIVTMVVIAGYSVKVFPTFESVLPVSRWPGVTQALYNLGFSL
YEGLWIKVLIFVAIFITILVFMSKNITGNFRDGFLDKLPPFNFVKHIAATEFLANMSMLLDSRVPFKEGLDIVDHKTTRW
LSSHLQRMKANMQEGLDYKQALDTNLLDKKMLLTMAVYSELPNFSDVMQKLAIEANINLHKKIATLAGVMKNISLITLAL
SVIWIFGAIFSLVDKLSSSL

Specific function: Probably involved in cholera toxin receptor (GM1) interaction in order to bring the cells within close proximity of the ganglioside for efficient toxin delivery

COG id: COG1459

COG function: function code NU; Type II secretory pathway, component PulF

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP F family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TCPE_VIBC3 (A5F382)

Other databases:

- EMBL:   AF325733
- EMBL:   CP000627
- EMBL:   CP001235
- STRING:   A5F382
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A0361
- eggNOG:   COG1459
- HOGENOM:   HBG264717
- OMA:   WHPFAEL
- ProtClustDB:   CLSK793813
- BioCyc:   VCHO345073:VC0395_A0361-MONOMER
- InterPro:   IPR018076
- InterPro:   IPR001992

Pfam domain/function: PF00482 GSPII_F

EC number: NA

Molecular weight: Translated: 38069; Mature: 38069

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: PS00874 T2SP_F

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x25a48060)-; HASH(0x25bd22e0)-; HASH(0x255cfc24)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIISKKYRLELYSMLVDLLNDNIPLYDALNKIQNEGVGIYDKNFIKSIELIKDRMKSNS
CCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCC
SLTDALTGLIPDKEVLMINVAENSGKISSGIAAIRKNIIDADEIKSKAISSMITPSVMLI
HHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
VTMVVIAGYSVKVFPTFESVLPVSRWPGVTQALYNLGFSLYEGLWIKVLIFVAIFITILV
HHHHHHCCCCEEEEECHHHHCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
FMSKNITGNFRDGFLDKLPPFNFVKHIAATEFLANMSMLLDSRVPFKEGLDIVDHKTTRW
HHHCCCCCCCHHCHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHH
LSSHLQRMKANMQEGLDYKQALDTNLLDKKMLLTMAVYSELPNFSDVMQKLAIEANINLH
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHH
KKIATLAGVMKNISLITLALSVIWIFGAIFSLVDKLSSSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH
>Mature Secondary Structure
MKIISKKYRLELYSMLVDLLNDNIPLYDALNKIQNEGVGIYDKNFIKSIELIKDRMKSNS
CCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCC
SLTDALTGLIPDKEVLMINVAENSGKISSGIAAIRKNIIDADEIKSKAISSMITPSVMLI
HHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
VTMVVIAGYSVKVFPTFESVLPVSRWPGVTQALYNLGFSLYEGLWIKVLIFVAIFITILV
HHHHHHCCCCEEEEECHHHHCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
FMSKNITGNFRDGFLDKLPPFNFVKHIAATEFLANMSMLLDSRVPFKEGLDIVDHKTTRW
HHHCCCCCCCHHCHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHH
LSSHLQRMKANMQEGLDYKQALDTNLLDKKMLLTMAVYSELPNFSDVMQKLAIEANINLH
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHH
KKIATLAGVMKNISLITLALSVIWIFGAIFSLVDKLSSSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11179381