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The map label for this gene is vibC

Identifier: 227080952

GI number: 227080952

Start: 786272

End: 787459

Strand: Reverse

Name: vibC

Synonym: VCM66_0731

Alternate gene names: 227080952

Gene position: 787459-786272 (Counterclockwise)

Preceding gene: 227080954

Following gene: 227080951

Centisome position: 27.22

GC content: 51.85

Gene sequence:

>1188_bases
ATGGTCATGAAGCGTGAAGTGGTCGGATACACCACTATGTCTGAACATCTGATCGACACCCAACTCACTCGCTCACCGTT
TTTCTTTGCCTCAGCAAACCAGTCAATGCTCGGTTGTGGGGTCGCTCATGCTTTTCAGCAAGCCATTCCTTTTGCTGAAC
TGGCTAACCAAGCCAAACAGCTTTTGCAGCAGGCCAAACGTGATGAGTGCGACAATCCATTACTGTTTGGCATAGTGCCG
TTTGACCCTAAAACACCGACTCGATTTATGATTCCGCGCACCTTATACGTGTCGAGTAGCCCGCGCTTAAACCGTCCAGC
TCACCTCACCCGCCAAGTGGCTAAACTCATCTCCTCGCCATCAGGAGAGCAATACAAGCAAGGTGTAAGCCATCTGCTCA
ACATGTTTCATCACTCTGGGCTGTCTAAAGTGGTGCTGTCGCGCGCCATTGAAATTGCCACCGAGCAAGAGATAGCACTA
CCGACCTTGCTGCGTAGTCTGCTCGCCATTAATCATCACGGCTATACTTTTGCGGCCAGCTTGGACGAGCAGAGAAAGCT
GATTGGTGCAAGCCCTGAACTCTTGGTCGCCAAACGCGGAAACTATCTCATCTCAAACCCACTGGCTGGCTCGCGCCCGA
GAAGTCAAGATGCCCAAGAAAATGCGCAGCGCCGTGCCTCACTGCTCAATACCGCCAAAGATTTGCATGAGCACGGTTTA
GTCGTCGAAGAAGTCGAGCGGATCATGAGCCGCTACTGTCGTAACTTGTACACCCCCATGGTGCCTTCTGTGATTGAAAC
CGAAACCATGCTGCACCTCTCAACCCTACTTGAAGGACAAGTCAGCGATCCTGAGGTTTGTGCTTTGCAAGTCGCCGCCG
ATTTACACCCCACTCCAGCCGTATGCGGTTTTCCGCGCGAATCGGCTTATCAAGCAATAAGAGAGCTGGAAGAATTTGAT
CGTGGCTACTTCACTGGCATGGTCGGTTGGTGTGATGCACGTGGCAATGGTGAGTGGGTAGTCACCATTCGCTGTGCTGA
AGTGGGATCTCACCAAATGAAGCTTTTTGCTGGCGCAGGGATTGTCGATGAGTCGTTACCACAAAGTGAACTGGAAGAGA
CGGGCGCGAAGATGAAAACCATCTTGGCTGCGGCAGGTATTGAGCTCAACGATGTGCTCACCGCTTAA

Upstream 100 bases:

>100_bases
TGCATTTATTAATAAAAACTATTCTCATTTGAATGTAAGCAAATGTAATCTGTTGCGCTTGGCAACGCAACCCATTCACT
CGCAAATATGGATAAACAAC

Downstream 100 bases:

>100_bases
GGAGATAAGAATGACAACCGATTTTACCCCTTGGCCAGAGGCACTTGCCGCTCAATACCGTCAGTTAGGCTACTGGCAAG
ATAAAACCTTGCTCGATTAC

Product: vibriobactin-specific isochorismate synthase

Products: NA

Alternate protein names: Isochorismate mutase

Number of amino acids: Translated: 395; Mature: 395

Protein sequence:

>395_residues
MVMKREVVGYTTMSEHLIDTQLTRSPFFFASANQSMLGCGVAHAFQQAIPFAELANQAKQLLQQAKRDECDNPLLFGIVP
FDPKTPTRFMIPRTLYVSSSPRLNRPAHLTRQVAKLISSPSGEQYKQGVSHLLNMFHHSGLSKVVLSRAIEIATEQEIAL
PTLLRSLLAINHHGYTFAASLDEQRKLIGASPELLVAKRGNYLISNPLAGSRPRSQDAQENAQRRASLLNTAKDLHEHGL
VVEEVERIMSRYCRNLYTPMVPSVIETETMLHLSTLLEGQVSDPEVCALQVAADLHPTPAVCGFPRESAYQAIRELEEFD
RGYFTGMVGWCDARGNGEWVVTIRCAEVGSHQMKLFAGAGIVDESLPQSELEETGAKMKTILAAAGIELNDVLTA

Sequences:

>Translated_395_residues
MVMKREVVGYTTMSEHLIDTQLTRSPFFFASANQSMLGCGVAHAFQQAIPFAELANQAKQLLQQAKRDECDNPLLFGIVP
FDPKTPTRFMIPRTLYVSSSPRLNRPAHLTRQVAKLISSPSGEQYKQGVSHLLNMFHHSGLSKVVLSRAIEIATEQEIAL
PTLLRSLLAINHHGYTFAASLDEQRKLIGASPELLVAKRGNYLISNPLAGSRPRSQDAQENAQRRASLLNTAKDLHEHGL
VVEEVERIMSRYCRNLYTPMVPSVIETETMLHLSTLLEGQVSDPEVCALQVAADLHPTPAVCGFPRESAYQAIRELEEFD
RGYFTGMVGWCDARGNGEWVVTIRCAEVGSHQMKLFAGAGIVDESLPQSELEETGAKMKTILAAAGIELNDVLTA
>Mature_395_residues
MVMKREVVGYTTMSEHLIDTQLTRSPFFFASANQSMLGCGVAHAFQQAIPFAELANQAKQLLQQAKRDECDNPLLFGIVP
FDPKTPTRFMIPRTLYVSSSPRLNRPAHLTRQVAKLISSPSGEQYKQGVSHLLNMFHHSGLSKVVLSRAIEIATEQEIAL
PTLLRSLLAINHHGYTFAASLDEQRKLIGASPELLVAKRGNYLISNPLAGSRPRSQDAQENAQRRASLLNTAKDLHEHGL
VVEEVERIMSRYCRNLYTPMVPSVIETETMLHLSTLLEGQVSDPEVCALQVAADLHPTPAVCGFPRESAYQAIRELEEFD
RGYFTGMVGWCDARGNGEWVVTIRCAEVGSHQMKLFAGAGIVDESLPQSELEETGAKMKTILAAAGIELNDVLTA

Specific function: Siderophore biosynthesis; enterobactin biosynthesis. [C]

COG id: COG1169

COG function: function code HQ; Isochorismate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismate synthase family

Homologues:

Organism=Escherichia coli, GI1786809, Length=391, Percent_Identity=38.618925831202, Blast_Score=244, Evalue=5e-66,
Organism=Escherichia coli, GI87082077, Length=363, Percent_Identity=26.7217630853994, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1787518, Length=275, Percent_Identity=28.7272727272727, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788114, Length=202, Percent_Identity=27.2277227722772, Blast_Score=74, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6320935, Length=235, Percent_Identity=31.4893617021277, Blast_Score=76, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): VIBC_VIBCH (O07898)

Other databases:

- EMBL:   U52150
- EMBL:   AE003852
- PIR:   A82283
- RefSeq:   NP_230422.1
- ProteinModelPortal:   O07898
- SMR:   O07898
- GeneID:   2615316
- GenomeReviews:   AE003852_GR
- KEGG:   vch:VC0773
- TIGR:   VC_0773
- HOGENOM:   HBG405459
- OMA:   RTMLLAM
- ProtClustDB:   CLSK793765
- BioCyc:   MetaCyc:VC0773-MONOMER
- BioCyc:   VCHO:VC0773-MONOMER
- BRENDA:   5.4.4.2
- InterPro:   IPR005801
- InterPro:   IPR015890
- InterPro:   IPR004561
- Gene3D:   G3DSA:3.60.120.10
- PANTHER:   PTHR11236:SF3
- PANTHER:   PTHR11236
- TIGRFAMs:   TIGR00543

Pfam domain/function: PF00425 Chorismate_bind; SSF56322 TRPE_1_chor_bd

EC number: =5.4.4.2

Molecular weight: Translated: 43595; Mature: 43595

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVMKREVVGYTTMSEHLIDTQLTRSPFFFASANQSMLGCGVAHAFQQAIPFAELANQAKQ
CCCCCHHHCCHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHCCHHHHHHHHHH
LLQQAKRDECDNPLLFGIVPFDPKTPTRFMIPRTLYVSSSPRLNRPAHLTRQVAKLISSP
HHHHHHHCCCCCCEEEEEECCCCCCCCEEECCEEEEECCCCCCCCCHHHHHHHHHHHCCC
SGEQYKQGVSHLLNMFHHSGLSKVVLSRAIEIATEQEIALPTLLRSLLAINHHGYTFAAS
CCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEEC
LDEQRKLIGASPELLVAKRGNYLISNPLAGSRPRSQDAQENAQRRASLLNTAKDLHEHGL
CHHHHHHHCCCCCEEEEECCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
VVEEVERIMSRYCRNLYTPMVPSVIETETMLHLSTLLEGQVSDPEVCALQVAADLHPTPA
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHEEHHHCCCCCCC
VCGFPRESAYQAIRELEEFDRGYFTGMVGWCDARGNGEWVVTIRCAEVGSHQMKLFAGAG
CCCCCHHHHHHHHHHHHHHCCCHHCCCEEEEECCCCCCEEEEEEEECCCCCCEEEEECCC
IVDESLPQSELEETGAKMKTILAAAGIELNDVLTA
CCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHCC
>Mature Secondary Structure
MVMKREVVGYTTMSEHLIDTQLTRSPFFFASANQSMLGCGVAHAFQQAIPFAELANQAKQ
CCCCCHHHCCHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHCCHHHHHHHHHH
LLQQAKRDECDNPLLFGIVPFDPKTPTRFMIPRTLYVSSSPRLNRPAHLTRQVAKLISSP
HHHHHHHCCCCCCEEEEEECCCCCCCCEEECCEEEEECCCCCCCCCHHHHHHHHHHHCCC
SGEQYKQGVSHLLNMFHHSGLSKVVLSRAIEIATEQEIALPTLLRSLLAINHHGYTFAAS
CCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEEC
LDEQRKLIGASPELLVAKRGNYLISNPLAGSRPRSQDAQENAQRRASLLNTAKDLHEHGL
CHHHHHHHCCCCCEEEEECCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
VVEEVERIMSRYCRNLYTPMVPSVIETETMLHLSTLLEGQVSDPEVCALQVAADLHPTPA
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHEEHHHCCCCCCC
VCGFPRESAYQAIRELEEFDRGYFTGMVGWCDARGNGEWVVTIRCAEVGSHQMKLFAGAG
CCCCCHHHHHHHHHHHHHHCCCHHCCCEEEEECCCCCCEEEEEEEECCCCCCEEEEECCC
IVDESLPQSELEETGAKMKTILAAAGIELNDVLTA
CCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371453; 10952301