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The map label for this gene is vibE

Identifier: 227080951

GI number: 227080951

Start: 784630

End: 786261

Strand: Reverse

Name: vibE

Synonym: VCM66_0730

Alternate gene names: 227080951

Gene position: 786261-784630 (Counterclockwise)

Preceding gene: 227080952

Following gene: 227080945

Centisome position: 27.18

GC content: 50.67

Gene sequence:

>1632_bases
ATGACAACCGATTTTACCCCTTGGCCAGAGGCACTTGCCGCTCAATACCGTCAGTTAGGCTACTGGCAAGATAAAACCTT
GCTCGATTACTTACAGCAAAGTGCTGAGCGTACCCCCAATGCGCTTGCGCTGGTCGGTGATAATCAGCAGTGGCGTTACC
AAGCCATGCTCGAACGTATTGAGCAGCTTGCTGCCGGATTTACCGAACTTGGGCTAGGCTGTGGCGATAACGTAGTGCTG
CAATTAGGCAATGTTGCGGAATTCTATTTATGCTTTTTCGCTTTGCTGCGCCAAGGCATACGACCGATTCTGGCGCTCCC
CGCTCACCGTTTAGCGGAAATTCGCTATTTTTGTCAGCACAGCCAAGCCAAAGCTTACCTCATTGATGGCGCTCAGCGAC
CATTTGACTATCAAGCTTTGGCGCAAGAGCTGCTTGCTTGCTGCCCAACACTGCAGACTGTGATTGTACGCGGCCAAACT
CGAGTGACAGATCCAAAATTTATTGAACTGGCGAGTTGCTACTCGGCATCATCTTGTCAAGCGAATGCCGACCCGAATCA
AATCGCCTTTTTCCAACTTTCCGGTGGCACAACGGGCACGCCCAAGCTCATCCCGCGTACCCACAATGATTACGCCTACA
GCGTAACCGCCAGTGTCGAAATTTGCCGTTTTGATCAGCATACTCGCTATCTGTGCGTACTGCCCGCGGCACATAACTTC
CCTCTCAGCTCCCCCGGCGCACTCGGTGTTTTCTGGGCGGGTGGCTGCGTTGTCCTCAGTCAAGATGCGTCACCCCAACA
CGCGTTTAAATTGATAGAACAGCATAAAATTACTGTTACCGCGTTGGTGCCGCCTTTAGCACTACTCTGGATGGATCACG
CAGAAAAAAGCACCTACGATCTCTCCTCATTGCACTTTGTGCAAGTAGGCGGGGCAAAATTTAGTGAAGCGGCCGCAAGG
CGTCTGCCGAAAGCGCTAGGCTGCCAATTGCAACAAGTGTTTGGAATGGCAGAAGGCTTGGTCAATTACACTCGTTTGGA
TGACAGTGCGGAACTGATTGCTACAACTCAAGGTCGGCCTATCTCCGCTCACGATCAATTACTGGTGGTTGATGAGCAGG
GTCAACCTGTCGCCAGCGGAGAAGAAGGGTATCTGTTAACCCAAGGTCCCTATACGATTCGCGGTTACTATCGAGCTGAT
CAGCACAATCAACGAGCGTTCAATGCACAAGGTTTTTACATCACCGGCGATAAAGTCAAACTCAGTAGCGAAGGTTACGT
GATTGTCACCGGAAGGGCCAAAGATCAGATCAATCGTGGAGGGGAAAAGATTGCCGCCGAAGAGGTGGAAAATCAGCTTT
TACATCATCCAGCAGTGCATGATGCCGCTTTAATTGCCATCAGTGACGAATACCTAGGAGAGCGCAGTTGCGCGGTGATC
GTACTCAAACCCGAGCAGTCGGTGAATACCATCCAGCTCAAACGTTTTTTACACCAAGCGGGGCTCGCCGATTACAAAAT
CCCAGATCAAATCCAATTTATCGATCAACTGCCCAAAACATCGGTTGGAAAAATCGATAAGAACGCGCTTCGTCGTCGAT
TTGACACGCTCGGCTTAGCGTTAATGTCGTGA

Upstream 100 bases:

>100_bases
CCACAAAGTGAACTGGAAGAGACGGGCGCGAAGATGAAAACCATCTTGGCTGCGGCAGGTATTGAGCTCAACGATGTGCT
CACCGCTTAAGGAGATAAGA

Downstream 100 bases:

>100_bases
AATCAATGTAAAGACAATGAGTGAGTAGAGGGAATCCTTGAATAAGCGGTTCCCTTCCTACTTAGAATCCGTGTGTTAAG
CAGGAAGGGAACTCGTTCAA

Product: vibriobactin-specific 2,3-dihydroxybenzoate-AMP ligase

Products: NA

Alternate protein names: Dihydroxybenzoic acid-activating enzyme

Number of amino acids: Translated: 543; Mature: 542

Protein sequence:

>543_residues
MTTDFTPWPEALAAQYRQLGYWQDKTLLDYLQQSAERTPNALALVGDNQQWRYQAMLERIEQLAAGFTELGLGCGDNVVL
QLGNVAEFYLCFFALLRQGIRPILALPAHRLAEIRYFCQHSQAKAYLIDGAQRPFDYQALAQELLACCPTLQTVIVRGQT
RVTDPKFIELASCYSASSCQANADPNQIAFFQLSGGTTGTPKLIPRTHNDYAYSVTASVEICRFDQHTRYLCVLPAAHNF
PLSSPGALGVFWAGGCVVLSQDASPQHAFKLIEQHKITVTALVPPLALLWMDHAEKSTYDLSSLHFVQVGGAKFSEAAAR
RLPKALGCQLQQVFGMAEGLVNYTRLDDSAELIATTQGRPISAHDQLLVVDEQGQPVASGEEGYLLTQGPYTIRGYYRAD
QHNQRAFNAQGFYITGDKVKLSSEGYVIVTGRAKDQINRGGEKIAAEEVENQLLHHPAVHDAALIAISDEYLGERSCAVI
VLKPEQSVNTIQLKRFLHQAGLADYKIPDQIQFIDQLPKTSVGKIDKNALRRRFDTLGLALMS

Sequences:

>Translated_543_residues
MTTDFTPWPEALAAQYRQLGYWQDKTLLDYLQQSAERTPNALALVGDNQQWRYQAMLERIEQLAAGFTELGLGCGDNVVL
QLGNVAEFYLCFFALLRQGIRPILALPAHRLAEIRYFCQHSQAKAYLIDGAQRPFDYQALAQELLACCPTLQTVIVRGQT
RVTDPKFIELASCYSASSCQANADPNQIAFFQLSGGTTGTPKLIPRTHNDYAYSVTASVEICRFDQHTRYLCVLPAAHNF
PLSSPGALGVFWAGGCVVLSQDASPQHAFKLIEQHKITVTALVPPLALLWMDHAEKSTYDLSSLHFVQVGGAKFSEAAAR
RLPKALGCQLQQVFGMAEGLVNYTRLDDSAELIATTQGRPISAHDQLLVVDEQGQPVASGEEGYLLTQGPYTIRGYYRAD
QHNQRAFNAQGFYITGDKVKLSSEGYVIVTGRAKDQINRGGEKIAAEEVENQLLHHPAVHDAALIAISDEYLGERSCAVI
VLKPEQSVNTIQLKRFLHQAGLADYKIPDQIQFIDQLPKTSVGKIDKNALRRRFDTLGLALMS
>Mature_542_residues
TTDFTPWPEALAAQYRQLGYWQDKTLLDYLQQSAERTPNALALVGDNQQWRYQAMLERIEQLAAGFTELGLGCGDNVVLQ
LGNVAEFYLCFFALLRQGIRPILALPAHRLAEIRYFCQHSQAKAYLIDGAQRPFDYQALAQELLACCPTLQTVIVRGQTR
VTDPKFIELASCYSASSCQANADPNQIAFFQLSGGTTGTPKLIPRTHNDYAYSVTASVEICRFDQHTRYLCVLPAAHNFP
LSSPGALGVFWAGGCVVLSQDASPQHAFKLIEQHKITVTALVPPLALLWMDHAEKSTYDLSSLHFVQVGGAKFSEAAARR
LPKALGCQLQQVFGMAEGLVNYTRLDDSAELIATTQGRPISAHDQLLVVDEQGQPVASGEEGYLLTQGPYTIRGYYRADQ
HNQRAFNAQGFYITGDKVKLSSEGYVIVTGRAKDQINRGGEKIAAEEVENQLLHHPAVHDAALIAISDEYLGERSCAVIV
LKPEQSVNTIQLKRFLHQAGLADYKIPDQIQFIDQLPKTSVGKIDKNALRRRFDTLGLALMS

Specific function: Activation of the carboxylate group of 2,3-dihydroxy- benzoate (DHB), via ATP-dependent PPi exchange reactions, to the acyladenylate, preparing that molecule for the final stages of vibriobactin synthesis

COG id: COG1021

COG function: function code Q; Peptide arylation enzymes

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family

Homologues:

Organism=Homo sapiens, GI115511026, Length=556, Percent_Identity=27.5179856115108, Blast_Score=146, Evalue=6e-35,
Organism=Homo sapiens, GI38505220, Length=527, Percent_Identity=25.0474383301708, Blast_Score=142, Evalue=7e-34,
Organism=Homo sapiens, GI42544132, Length=555, Percent_Identity=25.4054054054054, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI187761345, Length=390, Percent_Identity=29.2307692307692, Blast_Score=135, Evalue=1e-31,
Organism=Homo sapiens, GI187761343, Length=390, Percent_Identity=29.2307692307692, Blast_Score=135, Evalue=1e-31,
Organism=Homo sapiens, GI156151445, Length=552, Percent_Identity=23.731884057971, Blast_Score=135, Evalue=1e-31,
Organism=Homo sapiens, GI122937307, Length=524, Percent_Identity=25.9541984732824, Blast_Score=128, Evalue=2e-29,
Organism=Homo sapiens, GI157311624, Length=540, Percent_Identity=25.1851851851852, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI157311622, Length=540, Percent_Identity=25.1851851851852, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI58082049, Length=545, Percent_Identity=25.1376146788991, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI28416953, Length=547, Percent_Identity=24.1316270566728, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI195947378, Length=412, Percent_Identity=22.5728155339806, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI8923896, Length=377, Percent_Identity=24.1379310344828, Blast_Score=71, Evalue=3e-12,
Organism=Homo sapiens, GI253970489, Length=377, Percent_Identity=24.1379310344828, Blast_Score=71, Evalue=3e-12,
Organism=Escherichia coli, GI1786810, Length=538, Percent_Identity=48.1412639405204, Blast_Score=512, Evalue=1e-146,
Organism=Escherichia coli, GI145693145, Length=528, Percent_Identity=30.8712121212121, Blast_Score=227, Evalue=1e-60,
Organism=Escherichia coli, GI1788107, Length=536, Percent_Identity=25.5597014925373, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI221142682, Length=502, Percent_Identity=26.0956175298805, Blast_Score=123, Evalue=4e-29,
Organism=Escherichia coli, GI1790505, Length=366, Percent_Identity=24.8633879781421, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI1789201, Length=371, Percent_Identity=24.5283018867925, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1786529, Length=553, Percent_Identity=22.0614828209765, Blast_Score=86, Evalue=8e-18,
Organism=Escherichia coli, GI1788595, Length=495, Percent_Identity=23.6363636363636, Blast_Score=81, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17531443, Length=534, Percent_Identity=27.1535580524345, Blast_Score=154, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17557194, Length=514, Percent_Identity=25.2918287937743, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI17538037, Length=518, Percent_Identity=24.9034749034749, Blast_Score=128, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI17560308, Length=548, Percent_Identity=23.9051094890511, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI71994703, Length=545, Percent_Identity=24.0366972477064, Blast_Score=124, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI71994694, Length=545, Percent_Identity=24.0366972477064, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI71994690, Length=545, Percent_Identity=24.0366972477064, Blast_Score=123, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17558820, Length=510, Percent_Identity=23.7254901960784, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI32563687, Length=517, Percent_Identity=22.0502901353965, Blast_Score=95, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17559526, Length=367, Percent_Identity=24.2506811989101, Blast_Score=95, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI71983001, Length=551, Percent_Identity=22.8675136116152, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI71982997, Length=551, Percent_Identity=22.8675136116152, Blast_Score=89, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17560140, Length=523, Percent_Identity=22.9445506692161, Blast_Score=86, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI71985884, Length=315, Percent_Identity=25.0793650793651, Blast_Score=76, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6319699, Length=486, Percent_Identity=23.4567901234568, Blast_Score=129, Evalue=8e-31,
Organism=Saccharomyces cerevisiae, GI6319264, Length=559, Percent_Identity=21.824686940966, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24581924, Length=547, Percent_Identity=27.0566727605119, Blast_Score=162, Evalue=6e-40,
Organism=Drosophila melanogaster, GI18859661, Length=514, Percent_Identity=23.1517509727626, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI21355181, Length=541, Percent_Identity=23.6598890942699, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI19922652, Length=354, Percent_Identity=24.8587570621469, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24667955, Length=532, Percent_Identity=23.8721804511278, Blast_Score=93, Evalue=5e-19,
Organism=Drosophila melanogaster, GI62472339, Length=532, Percent_Identity=23.8721804511278, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI24648260, Length=283, Percent_Identity=22.9681978798587, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24667959, Length=372, Percent_Identity=25, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24656500, Length=490, Percent_Identity=23.469387755102, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21356947, Length=364, Percent_Identity=22.2527472527473, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI281365686, Length=349, Percent_Identity=22.9226361031519, Blast_Score=75, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24648257, Length=356, Percent_Identity=23.314606741573, Blast_Score=69, Evalue=8e-12,
Organism=Drosophila melanogaster, GI21356441, Length=476, Percent_Identity=21.8487394957983, Blast_Score=69, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24648253, Length=347, Percent_Identity=21.0374639769452, Blast_Score=66, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): VIBE_VIBCH (O07899)

Other databases:

- EMBL:   U52150
- EMBL:   AE003852
- PIR:   H82282
- RefSeq:   NP_230421.1
- ProteinModelPortal:   O07899
- SMR:   O07899
- GeneID:   2615315
- GenomeReviews:   AE003852_GR
- KEGG:   vch:VC0772
- TIGR:   VC_0772
- HOGENOM:   HBG547964
- OMA:   TEIGYFC
- ProtClustDB:   CLSK793764
- BioCyc:   MetaCyc:VC0772-MONOMER
- BioCyc:   VCHO:VC0772-MONOMER
- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR011963
- TIGRFAMs:   TIGR02275

Pfam domain/function: PF00501 AMP-binding

EC number: 6.3.2.-

Molecular weight: Translated: 60066; Mature: 59935

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x254c01dc)-;

Cys/Met content:

2.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTDFTPWPEALAAQYRQLGYWQDKTLLDYLQQSAERTPNALALVGDNQQWRYQAMLERI
CCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHH
EQLAAGFTELGLGCGDNVVLQLGNVAEFYLCFFALLRQGIRPILALPAHRLAEIRYFCQH
HHHHCCHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHC
SQAKAYLIDGAQRPFDYQALAQELLACCPTLQTVIVRGQTRVTDPKFIELASCYSASSCQ
CCCCEEEECCCCCCCCHHHHHHHHHHHCCHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCC
ANADPNQIAFFQLSGGTTGTPKLIPRTHNDYAYSVTASVEICRFDQHTRYLCVLPAAHNF
CCCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEEEEEEEEEEECCCCCCEEEEEECCCCC
PLSSPGALGVFWAGGCVVLSQDASPQHAFKLIEQHKITVTALVPPLALLWMDHAEKSTYD
CCCCCCCEEEEECCCEEEEECCCCHHHHHHHHHHCCEEEEEHHHHHHHHHHHCCCCCCCC
LSSLHFVQVGGAKFSEAAARRLPKALGCQLQQVFGMAEGLVNYTRLDDSAELIATTQGRP
CCCEEEEEECCCCHHHHHHHHCCHHHCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCC
ISAHDQLLVVDEQGQPVASGEEGYLLTQGPYTIRGYYRADQHNQRAFNAQGFYITGDKVK
CCCCCCEEEECCCCCCCCCCCCCEEEECCCEEEEEEEECCCCCCCEECCCCEEEECCEEE
LSSEGYVIVTGRAKDQINRGGEKIAAEEVENQLLHHPAVHDAALIAISDEYLGERSCAVI
ECCCCEEEEECCCHHHHHCCCHHHHHHHHHHHHHCCCCCCCEEEEEEEHHHCCCCCEEEE
VLKPEQSVNTIQLKRFLHQAGLADYKIPDQIQFIDQLPKTSVGKIDKNALRRRFDTLGLA
EECCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
LMS
HCH
>Mature Secondary Structure 
TTDFTPWPEALAAQYRQLGYWQDKTLLDYLQQSAERTPNALALVGDNQQWRYQAMLERI
CCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHH
EQLAAGFTELGLGCGDNVVLQLGNVAEFYLCFFALLRQGIRPILALPAHRLAEIRYFCQH
HHHHCCHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHC
SQAKAYLIDGAQRPFDYQALAQELLACCPTLQTVIVRGQTRVTDPKFIELASCYSASSCQ
CCCCEEEECCCCCCCCHHHHHHHHHHHCCHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCC
ANADPNQIAFFQLSGGTTGTPKLIPRTHNDYAYSVTASVEICRFDQHTRYLCVLPAAHNF
CCCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEEEEEEEEEEECCCCCCEEEEEECCCCC
PLSSPGALGVFWAGGCVVLSQDASPQHAFKLIEQHKITVTALVPPLALLWMDHAEKSTYD
CCCCCCCEEEEECCCEEEEECCCCHHHHHHHHHHCCEEEEEHHHHHHHHHHHCCCCCCCC
LSSLHFVQVGGAKFSEAAARRLPKALGCQLQQVFGMAEGLVNYTRLDDSAELIATTQGRP
CCCEEEEEECCCCHHHHHHHHCCHHHCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCC
ISAHDQLLVVDEQGQPVASGEEGYLLTQGPYTIRGYYRADQHNQRAFNAQGFYITGDKVK
CCCCCCEEEECCCCCCCCCCCCCEEEECCCEEEEEEEECCCCCCCEECCCCEEEECCEEE
LSSEGYVIVTGRAKDQINRGGEKIAAEEVENQLLHHPAVHDAALIAISDEYLGERSCAVI
ECCCCEEEEECCCHHHHHCCCHHHHHHHHHHHHHCCCCCCCEEEEEEEHHHCCCCCEEEE
VLKPEQSVNTIQLKRFLHQAGLADYKIPDQIQFIDQLPKTSVGKIDKNALRRRFDTLGLA
EECCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
LMS
HCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9371453; 10952301