Click here to switch to the map view.

The map label for this gene is amtB [C]

Identifier: 227080788

GI number: 227080788

Start: 595008

End: 596237

Strand: Direct

Name: amtB [C]

Synonym: VCM66_0565

Alternate gene names: 227080788

Gene position: 595008-596237 (Clockwise)

Preceding gene: 227080787

Following gene: 227080789

Centisome position: 20.57

GC content: 51.14

Gene sequence:

>1230_bases
ATGGAACTATCAATCACAGTTACTGAGCTGCGTTACGCACTCGATACATTTTTCTTCCTGATCTCAGGAGCGCTCGTCAT
GTGGATGGCGGCAGGTTTCGCTATGTTGGAAGCGGGTTTGGTTCGCTCCAAGAACACCACCGAAATTTTGACGAAAAACT
TTGTTCTGTATGCGATTGCTTGTACCACCTATTTGATCGTCGGCTACAACATCATGTATGTCGATAACACGGAAGGTGGC
TGGTTGCCTTCGTTTGGTGCGCTGATTGGCTCACAGGCCGATGGTGCTGACCACTCGCTCGAATCGGACTTCTTCTTCCA
AGTGGTGTTTGTGGCGACCGCGATGTCTGTCGTCTCTGGTGCCGTCGCGGAACGGATGAAACTCTGGGCATTTTTGATTT
TCTCCGCAGTATTGACCGCGTTCATCTATCCCGTTGAAGGTTACTGGACTTGGGGCGGTGGCTTCCTTTCAGCGGCAGGA
TTTAGTGATTTCGCAGGTTCCGGCATTGTGCATTTAGCGGGAGCTTCAGCGGCATTAGCAGGCGTTCTTCTGCTCGGGGC
TCGTAAAGGCAAATACGGCAAAAACGGCGAAATTTATCCAATTCCCGGTTCCAACATGCCATTGGCCACACTCGGTACGT
TTATTCTGTGGTTCGGCTGGTTTGGCTTTAACGGCGGCTCACAGTTGATGGTGTCGGATTTTGAAAATGCGACGTCTGTG
GGCAAAATTTTCCTCAATACCAATGCGGCTGCGGCGGCAGGGGCTATCGCTGCGCTTTTAGTGTGTAAAACCACTTGGGG
TAAAGCGGATCTCACCATGGTACTGAACGGTGCATTAGCGGGCTTAGTAGCGATTACGGCCGATCCTCTCTCTCCTTCAC
CGCTTTATTCGGTAGCGATTGGCGCAGTATCTGGCTCTCTGGTGGTGTTTAGCATTATTGGTCTCGATAAGCTGAAAATT
GATGATCCAGTGGGTGCGATTTCCGTCCATGGCGTGTGTGGTTTCTTTGGTCTGATGGTGGTACCACTGAGCAACGCTGA
TGCAACCTTTGGCGCACAATTACTCGGTGCTGCGGTGATCTTCGCATGGGTGTTTGGTGCAAGTCTGGTGGTTTGGGGCG
TGCTTAAAGCGACGATGGGGATCCGCGTCTCTGAAGAAGAAGAGATGGAAGGGATGGACATGCACGATTGTGGTGTCGGT
GCTTACCCAGAGTTTGTTACTGTTAAGTGA

Upstream 100 bases:

>100_bases
TGATGGCAAAATTTTTGTGTATGACCTAAGCCAAGCGGTACGTATCCGTACTGGCGAAATGGATTCAGAAGCCCTTTAAA
TTCAAGGATGAGATTTGATT

Downstream 100 bases:

>100_bases
AATATCATTTTACAAATTGAACCGAACAAAACCTGCCGTAAAGGCGGGTTTTGTTTTTTTGCCAATTGTTTTCTTATTGA
ACATTCCTATAATACATTGC

Product: putative ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 409; Mature: 409

Protein sequence:

>409_residues
MELSITVTELRYALDTFFFLISGALVMWMAAGFAMLEAGLVRSKNTTEILTKNFVLYAIACTTYLIVGYNIMYVDNTEGG
WLPSFGALIGSQADGADHSLESDFFFQVVFVATAMSVVSGAVAERMKLWAFLIFSAVLTAFIYPVEGYWTWGGGFLSAAG
FSDFAGSGIVHLAGASAALAGVLLLGARKGKYGKNGEIYPIPGSNMPLATLGTFILWFGWFGFNGGSQLMVSDFENATSV
GKIFLNTNAAAAAGAIAALLVCKTTWGKADLTMVLNGALAGLVAITADPLSPSPLYSVAIGAVSGSLVVFSIIGLDKLKI
DDPVGAISVHGVCGFFGLMVVPLSNADATFGAQLLGAAVIFAWVFGASLVVWGVLKATMGIRVSEEEEMEGMDMHDCGVG
AYPEFVTVK

Sequences:

>Translated_409_residues
MELSITVTELRYALDTFFFLISGALVMWMAAGFAMLEAGLVRSKNTTEILTKNFVLYAIACTTYLIVGYNIMYVDNTEGG
WLPSFGALIGSQADGADHSLESDFFFQVVFVATAMSVVSGAVAERMKLWAFLIFSAVLTAFIYPVEGYWTWGGGFLSAAG
FSDFAGSGIVHLAGASAALAGVLLLGARKGKYGKNGEIYPIPGSNMPLATLGTFILWFGWFGFNGGSQLMVSDFENATSV
GKIFLNTNAAAAAGAIAALLVCKTTWGKADLTMVLNGALAGLVAITADPLSPSPLYSVAIGAVSGSLVVFSIIGLDKLKI
DDPVGAISVHGVCGFFGLMVVPLSNADATFGAQLLGAAVIFAWVFGASLVVWGVLKATMGIRVSEEEEMEGMDMHDCGVG
AYPEFVTVK
>Mature_409_residues
MELSITVTELRYALDTFFFLISGALVMWMAAGFAMLEAGLVRSKNTTEILTKNFVLYAIACTTYLIVGYNIMYVDNTEGG
WLPSFGALIGSQADGADHSLESDFFFQVVFVATAMSVVSGAVAERMKLWAFLIFSAVLTAFIYPVEGYWTWGGGFLSAAG
FSDFAGSGIVHLAGASAALAGVLLLGARKGKYGKNGEIYPIPGSNMPLATLGTFILWFGWFGFNGGSQLMVSDFENATSV
GKIFLNTNAAAAAGAIAALLVCKTTWGKADLTMVLNGALAGLVAITADPLSPSPLYSVAIGAVSGSLVVFSIIGLDKLKI
DDPVGAISVHGVCGFFGLMVVPLSNADATFGAQLLGAAVIFAWVFGASLVVWGVLKATMGIRVSEEEEMEGMDMHDCGVG
AYPEFVTVK

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=417, Percent_Identity=31.4148681055156, Blast_Score=156, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI17550180, Length=443, Percent_Identity=36.117381489842, Blast_Score=204, Evalue=5e-53,
Organism=Caenorhabditis elegans, GI17550182, Length=426, Percent_Identity=33.5680751173709, Blast_Score=184, Evalue=7e-47,
Organism=Caenorhabditis elegans, GI32564042, Length=427, Percent_Identity=26.9320843091335, Blast_Score=140, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI133931100, Length=341, Percent_Identity=30.4985337243402, Blast_Score=131, Evalue=8e-31,
Organism=Saccharomyces cerevisiae, GI6325396, Length=421, Percent_Identity=29.2161520190024, Blast_Score=118, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6321559, Length=421, Percent_Identity=28.0285035629454, Blast_Score=112, Evalue=7e-26,
Organism=Saccharomyces cerevisiae, GI6324187, Length=417, Percent_Identity=28.2973621103118, Blast_Score=110, Evalue=4e-25,
Organism=Drosophila melanogaster, GI161078314, Length=404, Percent_Identity=30.9405940594059, Blast_Score=164, Evalue=8e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 42907; Mature: 42907

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELSITVTELRYALDTFFFLISGALVMWMAAGFAMLEAGLVRSKNTTEILTKNFVLYAIA
CCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
CTTYLIVGYNIMYVDNTEGGWLPSFGALIGSQADGADHSLESDFFFQVVFVATAMSVVSG
HHHHHHHCEEEEEEECCCCCCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
AVAERMKLWAFLIFSAVLTAFIYPVEGYWTWGGGFLSAAGFSDFAGSGIVHLAGASAALA
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHCCCCCCCCCCCEEEECCCHHHHH
GVLLLGARKGKYGKNGEIYPIPGSNMPLATLGTFILWFGWFGFNGGSQLMVSDFENATSV
HHHHHCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCC
GKIFLNTNAAAAAGAIAALLVCKTTWGKADLTMVLNGALAGLVAITADPLSPSPLYSVAI
EEEEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHEEEEEECCCCCCCHHHHHH
GAVSGSLVVFSIIGLDKLKIDDPVGAISVHGVCGFFGLMVVPLSNADATFGAQLLGAAVI
HHCCCCEEEEEEECCCEEEECCCCCEEEHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHH
FAWVFGASLVVWGVLKATMGIRVSEEEEMEGMDMHDCGVGAYPEFVTVK
HHHHHHHHHHHHHHHHHHHCCEECCHHHCCCCCCCCCCCCCCCCEEEEC
>Mature Secondary Structure
MELSITVTELRYALDTFFFLISGALVMWMAAGFAMLEAGLVRSKNTTEILTKNFVLYAIA
CCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
CTTYLIVGYNIMYVDNTEGGWLPSFGALIGSQADGADHSLESDFFFQVVFVATAMSVVSG
HHHHHHHCEEEEEEECCCCCCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
AVAERMKLWAFLIFSAVLTAFIYPVEGYWTWGGGFLSAAGFSDFAGSGIVHLAGASAALA
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHCCCCCCCCCCCEEEECCCHHHHH
GVLLLGARKGKYGKNGEIYPIPGSNMPLATLGTFILWFGWFGFNGGSQLMVSDFENATSV
HHHHHCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCC
GKIFLNTNAAAAAGAIAALLVCKTTWGKADLTMVLNGALAGLVAITADPLSPSPLYSVAI
EEEEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHEEEEEECCCCCCCHHHHHH
GAVSGSLVVFSIIGLDKLKIDDPVGAISVHGVCGFFGLMVVPLSNADATFGAQLLGAAVI
HHCCCCEEEEEEECCCEEEECCCCCEEEHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHH
FAWVFGASLVVWGVLKATMGIRVSEEEEMEGMDMHDCGVGAYPEFVTVK
HHHHHHHHHHHHHHHHHHHCCEECCHHHCCCCCCCCCCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]