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The map label for this gene is alaS [H]

Identifier: 227080728

GI number: 227080728

Start: 533971

End: 536553

Strand: Direct

Name: alaS [H]

Synonym: VCM66_0503

Alternate gene names: 227080728

Gene position: 533971-536553 (Clockwise)

Preceding gene: 227080727

Following gene: 227080729

Centisome position: 18.46

GC content: 50.6

Gene sequence:

>2583_bases
ATGTTTATGAGCACGGATGAGGTGCGCCGCGCGTTCCTCTCATTCTTTGAAAGCAAAGGTCACCAAATTGTAGAAAGTTC
TTCTCTGGTGCCTGCTAACGATCCGACCCTGTTGTTTACCAACGCAGGGATGAACCAATTTAAAGACTGTTTCTTGGGCT
TGGAAAAGCGCGCCTACACTCGCGCCACGACCGCTCAACGTTGTGTCCGCGCGGGCGGTAAGCACAACGACCTAGAAAAC
GTCGGTTTCACCGCACGTCACCACACTTTCTTCGAAATGCTGGGTAACTTCAGTTTCGGTGACTATTTCAAAGAAGATGC
GATCCAGTATGCATGGGAGTTTCTGACTGACGTTCTGCAACTGCCGAAAGAGCGCTTGTTAGTCACGGTTTATGAAACCG
ATGACGAAGCCTTTGATATTTGGAATAAAAAAGTCGGCATTCCTGCTGACCGCATTATTCGTATCGGCGATAAAAAAGGT
GGCAAGAAGTTCGATTCGGATAACTTCTGGCAGATGGGTGACACCGGTCCTTGCGGTCCATGTACTGAAATCTTCTACGA
CCACGGTGACCACATTTGGGGTGGACCTCCAGGCTCACCTGAGGAAGATGGCGATCGTTTCATCGAAATCTGGAACAACG
TTTTCATGCAGTTCAACCGTCATGCGGATGGCACGATGGAGCCGCTACCTAAGCCGTCTGTGGACACGGGCATGGGTATT
GAGCGTATTTCCGCGATCATGCAAGGCGTGCACTCCAACTACGAAATCGACGTATTCCAAACGCTGATCAAAGCAGCCGC
AGACGCAATTGGTTACCAAGATCTGACTAATCAGTCTCTGCGCGTTGTCGCGGATCACATCCGTTCATGTGCCTTTTTGA
TTGTGGATGGCGTGATGCCGTCTAACGAAGGTCGTGGTTATGTACTGCGTCGTATCATTCGTCGTGCCGTTCGTCATGGC
AATAAGCTCGGTGCGCAAGGTGCATTCTTCCACAAGTTGGTGGGACCATTGGCGGAAGTGATGGGTACTGCAGGCGTTGA
GCTGAAAAAGCAGCAAGCTCTGGTTGAAAAAGTGCTACGTATCGAAGAAGAAAACTTTGGACGTACGCTGGATCGCGGCA
TGAGCATTCTGAATGACGCGCTGGATCAACTATCAGGCCAAGTGCTGGATGGTGAAACCGTCTTCAAACTCTACGACACC
TACGGCTTCCCTGCGGATTTAACCAATGATGTGGCGCGTGAGCGTGGCTTTAGCATTGATGAAGCGGGTTTTGAACAAGC
GATGGAAGAGCAGCGTCAACGCGCTCGTGAGGCTGGCCAATTTGGTACGGATTATAATTCGCTGATCAAATCCGCCACCA
ATACCGAATTCTGCGGTTACACGGCGAGTCGTGGCCAAAGTGTGGTTCGCGAAATGTTCGTGGAGGGCGCGGAAGTTTCT
ACGCTGTCAGCTGGTGATAAAGCCATCATCGTACTAGACAACACGCCATTCTACGCAGAATCAGGTGGTCAATGCGGCGA
TACTGGCGTTTTGAAAACCGACGCAGGTATTTTCCATGTTGAAGATACTCAAAAATTAGGTAACGCGATTGCGCATCACG
GTGTGATCGCCCAAGGCGTATTGGCGACAGGCGATCAGGTGGATGCCATCGTCGATGAAAAGCGCCGTGCGGCAATTTCG
CTGAACCACTCGGCGACTCACTTGTTGCATGCTGCACTACGTAAAGTGCTGGGTGAACACGTGGCGCAGAAAGGCTCTCT
AGTGCGTGCTGAAACACTGCGTTTTGACTTCTCACATCTGGAAGCGATGACCGCTGCAGAGATTAAAGAAGTAGAACGTT
TAGTGAACCAAGAAGTTCGTCGTAACCACAGTATCGAAACCAACATCATGAATATTGATGAAGCGAAAGCGAAAGGTGCG
ATGGCGCTGTTTGGTGAGAAATATGACGACCAAGTTCGTGTGCTCTCAATGGGAGATTTCTCAACTGAGCTGTGTGGTGG
TATCCACGCGAGCAACACGGGTGATATTGGTCTGTTTAAGATCATCTCTGAAGGTGGTATTGCTGCGGGTATTCGCCGTA
TTGAAGCGGTGACTGGTGAAGGCGCATTAGATTACCTCGATGCACAGCAAGCACAGCATGATGCCAAAGTGTCTGAAATG
GCGGCAAAAGCCAAACTGCTGGAGAAAGAGATCCAACAGTTGAAAGATAAGCTGGCGGCGAAAGAGAGCGCGGGTCTTAT
CAACCAAGTTAAGCAGATCGCGGGTGTTAACGTACTGGTTGCGCAGCTCAACGGTGCAGACAACAAAGCGCTACGTGGCA
TGGTAGATGATCTCAAAAACCAATTAAGCAGCGGCATCATCATGCTGGGTAATGTGGCTGAAGGCAAAGTTGGCCTAATT
GCGGGCGTAACCAACGATTTGACCAATAAAGTCAAAGCGGGTGAGCTGGTCAACATGGTCGCACTACAAGTCGGTGGTAA
GGGTGGTGGCCGCCCTGACATGGCTCAAGCTGGAGGGACAGATGCTCACGCTCTGCCATCGGCCTTAGAGTCTGTCGACG
CTTGGATTGCCGAGCGTCTATAA

Upstream 100 bases:

>100_bases
GCCACACATTCAGTTTGCGGAAGAGAAGAAACTAGGCGAACTGTTATCCTTGCTTTACAATAGCGCAAAATTCTAACTTG
AGTATTTCAGGAAGAGCTGC

Downstream 100 bases:

>100_bases
CAGTGATAAATAGATTACGCTCAATCAATATTTGTTGATTGAGCGTAATTTTATCTGGTCTACCAAGTGGTTTAATTGGC
GCAAATATAGACTAAGCCAA

Product: alanyl-tRNA synthetase

Products: NA

Alternate protein names: Alanine--tRNA ligase; AlaRS [H]

Number of amino acids: Translated: 860; Mature: 860

Protein sequence:

>860_residues
MFMSTDEVRRAFLSFFESKGHQIVESSSLVPANDPTLLFTNAGMNQFKDCFLGLEKRAYTRATTAQRCVRAGGKHNDLEN
VGFTARHHTFFEMLGNFSFGDYFKEDAIQYAWEFLTDVLQLPKERLLVTVYETDDEAFDIWNKKVGIPADRIIRIGDKKG
GKKFDSDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRFIEIWNNVFMQFNRHADGTMEPLPKPSVDTGMGI
ERISAIMQGVHSNYEIDVFQTLIKAAADAIGYQDLTNQSLRVVADHIRSCAFLIVDGVMPSNEGRGYVLRRIIRRAVRHG
NKLGAQGAFFHKLVGPLAEVMGTAGVELKKQQALVEKVLRIEEENFGRTLDRGMSILNDALDQLSGQVLDGETVFKLYDT
YGFPADLTNDVARERGFSIDEAGFEQAMEEQRQRAREAGQFGTDYNSLIKSATNTEFCGYTASRGQSVVREMFVEGAEVS
TLSAGDKAIIVLDNTPFYAESGGQCGDTGVLKTDAGIFHVEDTQKLGNAIAHHGVIAQGVLATGDQVDAIVDEKRRAAIS
LNHSATHLLHAALRKVLGEHVAQKGSLVRAETLRFDFSHLEAMTAAEIKEVERLVNQEVRRNHSIETNIMNIDEAKAKGA
MALFGEKYDDQVRVLSMGDFSTELCGGIHASNTGDIGLFKIISEGGIAAGIRRIEAVTGEGALDYLDAQQAQHDAKVSEM
AAKAKLLEKEIQQLKDKLAAKESAGLINQVKQIAGVNVLVAQLNGADNKALRGMVDDLKNQLSSGIIMLGNVAEGKVGLI
AGVTNDLTNKVKAGELVNMVALQVGGKGGGRPDMAQAGGTDAHALPSALESVDAWIAERL

Sequences:

>Translated_860_residues
MFMSTDEVRRAFLSFFESKGHQIVESSSLVPANDPTLLFTNAGMNQFKDCFLGLEKRAYTRATTAQRCVRAGGKHNDLEN
VGFTARHHTFFEMLGNFSFGDYFKEDAIQYAWEFLTDVLQLPKERLLVTVYETDDEAFDIWNKKVGIPADRIIRIGDKKG
GKKFDSDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRFIEIWNNVFMQFNRHADGTMEPLPKPSVDTGMGI
ERISAIMQGVHSNYEIDVFQTLIKAAADAIGYQDLTNQSLRVVADHIRSCAFLIVDGVMPSNEGRGYVLRRIIRRAVRHG
NKLGAQGAFFHKLVGPLAEVMGTAGVELKKQQALVEKVLRIEEENFGRTLDRGMSILNDALDQLSGQVLDGETVFKLYDT
YGFPADLTNDVARERGFSIDEAGFEQAMEEQRQRAREAGQFGTDYNSLIKSATNTEFCGYTASRGQSVVREMFVEGAEVS
TLSAGDKAIIVLDNTPFYAESGGQCGDTGVLKTDAGIFHVEDTQKLGNAIAHHGVIAQGVLATGDQVDAIVDEKRRAAIS
LNHSATHLLHAALRKVLGEHVAQKGSLVRAETLRFDFSHLEAMTAAEIKEVERLVNQEVRRNHSIETNIMNIDEAKAKGA
MALFGEKYDDQVRVLSMGDFSTELCGGIHASNTGDIGLFKIISEGGIAAGIRRIEAVTGEGALDYLDAQQAQHDAKVSEM
AAKAKLLEKEIQQLKDKLAAKESAGLINQVKQIAGVNVLVAQLNGADNKALRGMVDDLKNQLSSGIIMLGNVAEGKVGLI
AGVTNDLTNKVKAGELVNMVALQVGGKGGGRPDMAQAGGTDAHALPSALESVDAWIAERL
>Mature_860_residues
MFMSTDEVRRAFLSFFESKGHQIVESSSLVPANDPTLLFTNAGMNQFKDCFLGLEKRAYTRATTAQRCVRAGGKHNDLEN
VGFTARHHTFFEMLGNFSFGDYFKEDAIQYAWEFLTDVLQLPKERLLVTVYETDDEAFDIWNKKVGIPADRIIRIGDKKG
GKKFDSDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRFIEIWNNVFMQFNRHADGTMEPLPKPSVDTGMGI
ERISAIMQGVHSNYEIDVFQTLIKAAADAIGYQDLTNQSLRVVADHIRSCAFLIVDGVMPSNEGRGYVLRRIIRRAVRHG
NKLGAQGAFFHKLVGPLAEVMGTAGVELKKQQALVEKVLRIEEENFGRTLDRGMSILNDALDQLSGQVLDGETVFKLYDT
YGFPADLTNDVARERGFSIDEAGFEQAMEEQRQRAREAGQFGTDYNSLIKSATNTEFCGYTASRGQSVVREMFVEGAEVS
TLSAGDKAIIVLDNTPFYAESGGQCGDTGVLKTDAGIFHVEDTQKLGNAIAHHGVIAQGVLATGDQVDAIVDEKRRAAIS
LNHSATHLLHAALRKVLGEHVAQKGSLVRAETLRFDFSHLEAMTAAEIKEVERLVNQEVRRNHSIETNIMNIDEAKAKGA
MALFGEKYDDQVRVLSMGDFSTELCGGIHASNTGDIGLFKIISEGGIAAGIRRIEAVTGEGALDYLDAQQAQHDAKVSEM
AAKAKLLEKEIQQLKDKLAAKESAGLINQVKQIAGVNVLVAQLNGADNKALRGMVDDLKNQLSSGIIMLGNVAEGKVGLI
AGVTNDLTNKVKAGELVNMVALQVGGKGGGRPDMAQAGGTDAHALPSALESVDAWIAERL

Specific function: Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction:alanine is first activated by ATP to form Ala- AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editin

COG id: COG0013

COG function: function code J; Alanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI109148542, Length=980, Percent_Identity=37.7551020408163, Blast_Score=542, Evalue=1e-154,
Organism=Homo sapiens, GI38569417, Length=907, Percent_Identity=35.2811466372657, Blast_Score=447, Evalue=1e-125,
Organism=Escherichia coli, GI1789048, Length=876, Percent_Identity=68.37899543379, Blast_Score=1216, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17506981, Length=979, Percent_Identity=37.5893769152196, Blast_Score=550, Evalue=1e-157,
Organism=Caenorhabditis elegans, GI17536681, Length=742, Percent_Identity=34.366576819407, Blast_Score=395, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6324911, Length=958, Percent_Identity=38.9352818371608, Blast_Score=577, Evalue=1e-165,
Organism=Drosophila melanogaster, GI24582809, Length=957, Percent_Identity=37.7220480668757, Blast_Score=548, Evalue=1e-156,
Organism=Drosophila melanogaster, GI45552267, Length=957, Percent_Identity=37.7220480668757, Blast_Score=548, Evalue=1e-156,
Organism=Drosophila melanogaster, GI24658214, Length=853, Percent_Identity=31.1840562719812, Blast_Score=340, Evalue=4e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002318
- InterPro:   IPR018162
- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR023033
- InterPro:   IPR003156
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF02272 DHHA1; PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: =6.1.1.7 [H]

Molecular weight: Translated: 93828; Mature: 93828

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS50860 AA_TRNA_LIGASE_II_ALA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFMSTDEVRRAFLSFFESKGHQIVESSSLVPANDPTLLFTNAGMNQFKDCFLGLEKRAYT
CCCCHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHH
RATTAQRCVRAGGKHNDLENVGFTARHHTFFEMLGNFSFGDYFKEDAIQYAWEFLTDVLQ
HHHHHHHHHHCCCCCCCHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
LPKERLLVTVYETDDEAFDIWNKKVGIPADRIIRIGDKKGGKKFDSDNFWQMGDTGPCGP
CCHHHEEEEEEECCCHHHHHHCCCCCCCHHHEEEECCCCCCCCCCCCCCEECCCCCCCCH
CTEIFYDHGDHIWGGPPGSPEEDGDRFIEIWNNVFMQFNRHADGTMEPLPKPSVDTGMGI
HHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCH
ERISAIMQGVHSNYEIDVFQTLIKAAADAIGYQDLTNQSLRVVADHIRSCAFLIVDGVMP
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCHHHHCCHHHHHHHHHHHHHHHHHEECCCC
SNEGRGYVLRRIIRRAVRHGNKLGAQGAFFHKLVGPLAEVMGTAGVELKKQQALVEKVLR
CCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
IEEENFGRTLDRGMSILNDALDQLSGQVLDGETVFKLYDTYGFPADLTNDVARERGFSID
HHHHHHHHHHHHHHHHHHHHHHHHCCCEECCHHHHHHHHHCCCCCHHHHHHHHHCCCCCC
EAGFEQAMEEQRQRAREAGQFGTDYNSLIKSATNTEFCGYTASRGQSVVREMFVEGAEVS
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCEE
TLSAGDKAIIVLDNTPFYAESGGQCGDTGVLKTDAGIFHVEDTQKLGNAIAHHGVIAQGV
EECCCCEEEEEECCCCCCCCCCCCCCCCCEEEECCCEEEECHHHHHHHHHHHCCHHHHHH
LATGDQVDAIVDEKRRAAISLNHSATHLLHAALRKVLGEHVAQKGSLVRAETLRFDFSHL
HCCCCHHHHHHHHHHHHEEEECCHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHH
EAMTAAEIKEVERLVNQEVRRNHSIETNIMNIDEAKAKGAMALFGEKYDDQVRVLSMGDF
HHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCHHHHHCCHHHHHCCCCCCCEEEEEECCC
STELCGGIHASNTGDIGLFKIISEGGIAAGIRRIEAVTGEGALDYLDAQQAQHDAKVSEM
HHHHHCCCCCCCCCCEEHHEEHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
AAKAKLLEKEIQQLKDKLAAKESAGLINQVKQIAGVNVLVAQLNGADNKALRGMVDDLKN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHH
QLSSGIIMLGNVAEGKVGLIAGVTNDLTNKVKAGELVNMVALQVGGKGGGRPDMAQAGGT
HHHCCEEEEECCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHCCCC
DAHALPSALESVDAWIAERL
CHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFMSTDEVRRAFLSFFESKGHQIVESSSLVPANDPTLLFTNAGMNQFKDCFLGLEKRAYT
CCCCHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHH
RATTAQRCVRAGGKHNDLENVGFTARHHTFFEMLGNFSFGDYFKEDAIQYAWEFLTDVLQ
HHHHHHHHHHCCCCCCCHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
LPKERLLVTVYETDDEAFDIWNKKVGIPADRIIRIGDKKGGKKFDSDNFWQMGDTGPCGP
CCHHHEEEEEEECCCHHHHHHCCCCCCCHHHEEEECCCCCCCCCCCCCCEECCCCCCCCH
CTEIFYDHGDHIWGGPPGSPEEDGDRFIEIWNNVFMQFNRHADGTMEPLPKPSVDTGMGI
HHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCH
ERISAIMQGVHSNYEIDVFQTLIKAAADAIGYQDLTNQSLRVVADHIRSCAFLIVDGVMP
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCHHHHCCHHHHHHHHHHHHHHHHHEECCCC
SNEGRGYVLRRIIRRAVRHGNKLGAQGAFFHKLVGPLAEVMGTAGVELKKQQALVEKVLR
CCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
IEEENFGRTLDRGMSILNDALDQLSGQVLDGETVFKLYDTYGFPADLTNDVARERGFSID
HHHHHHHHHHHHHHHHHHHHHHHHCCCEECCHHHHHHHHHCCCCCHHHHHHHHHCCCCCC
EAGFEQAMEEQRQRAREAGQFGTDYNSLIKSATNTEFCGYTASRGQSVVREMFVEGAEVS
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCEE
TLSAGDKAIIVLDNTPFYAESGGQCGDTGVLKTDAGIFHVEDTQKLGNAIAHHGVIAQGV
EECCCCEEEEEECCCCCCCCCCCCCCCCCEEEECCCEEEECHHHHHHHHHHHCCHHHHHH
LATGDQVDAIVDEKRRAAISLNHSATHLLHAALRKVLGEHVAQKGSLVRAETLRFDFSHL
HCCCCHHHHHHHHHHHHEEEECCHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHH
EAMTAAEIKEVERLVNQEVRRNHSIETNIMNIDEAKAKGAMALFGEKYDDQVRVLSMGDF
HHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCHHHHHCCHHHHHCCCCCCCEEEEEECCC
STELCGGIHASNTGDIGLFKIISEGGIAAGIRRIEAVTGEGALDYLDAQQAQHDAKVSEM
HHHHHCCCCCCCCCCEEHHEEHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
AAKAKLLEKEIQQLKDKLAAKESAGLINQVKQIAGVNVLVAQLNGADNKALRGMVDDLKN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHH
QLSSGIIMLGNVAEGKVGLIAGVTNDLTNKVKAGELVNMVALQVGGKGGGRPDMAQAGGT
HHHCCEEEEECCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHCCCC
DAHALPSALESVDAWIAERL
CHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA