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The map label for this gene is tyrS-1 [H]

Identifier: 227080675

GI number: 227080675

Start: 479223

End: 480527

Strand: Direct

Name: tyrS-1 [H]

Synonym: VCM66_0450

Alternate gene names: 227080675

Gene position: 479223-480527 (Clockwise)

Preceding gene: 227080674

Following gene: 227080683

Centisome position: 16.57

GC content: 50.88

Gene sequence:

>1305_bases
TTGCCAAGACTTTTAGGAGGCAGAATGAATTCACAGCAACTTTTTGATGATTTACAGCAGCGCGGTTTGATCGCGCAAAG
CACACCTCTGGAGGCGCTGGTTGAGCGCTTTGCCACACCGCAAGTGCTGTATTGCGGTTTTGACCCCACTGCAGGCAGTT
TGCACATCGGCCATTTGGTGCCGTTGCTGATGCTTAAGCGTTTTCAAAACGCCGGACATCAAGCGATTGCGCTTATCGGG
GGGGCCACAGGCATGATTGGCGATCCAAGCTTCAAAGCCAACGAGCGGGTGCTCAATAGTGCGCAAACTGTACAGCAGTG
GGTGAGTGATTTAAGCCAGCAAATCACGCAATTGATGACCTCGCAGGGCGAGAACTGCGCATCGCTGATGGCTGTCAATA
ATGCCGATTGGATCGGTAAGATCAATCTGATCGATTTTTTCCGCGATGTGGGCAAGCACTTTTCCGTCAATGCCATGATC
AATCGTGAATCGGTAAAGCAGCGTTTGGCGCGTCCGGATCAAGGCATCTCTTTTACCGAGTTCAGTTACTCTTTGCTGCA
ATCTTATGACTTTGCGCATCTCAATCGTGAGTTTGGCTGCGCACTGCAAATTGGTGGCAATGATCAGTGGGGCAATATTG
TCAGCGGAATTGATTTGACCCGTCGCCTGAATGGGGTTGAGGTGTGTGGTTTAACTCTGCCGCTCATTACCAAATCCGAT
GGTACTAAATTTGGCAAAACCGAAAGTGGTGCAGTGTGGCTTGAGAGCAGCAAAACCTCGCCGTATCGCTTTTACCAGTT
TTGGTTAAACAGTGATGATGCCGATGTGTACCGCTTCTTGGCGTATTACACCTTTTTGAGCACGGCTGAGATTGAGGAAA
TCCGTCAAGCCGATGCGCTACGTGCAGGCAAACCACAAGCGCAGCAGATTTTGGCCGAACACGTGACCCGTTTTGTGCAT
GGTGAAGAGGGACTCGCTGCGGCGCAGCGGATTACTCAAGCGCTGTTTAGCGGGGACGTCAGCTATCTCAGCTTAAGTGA
GTTGGCTCAGTTAGAACTGGATGGTTTGCCTTGTGTGGCGGCATCACGCACCGATTCCTTACTCGATTTGCTGCTTAGCT
CAGGTTTAGCCAGTTCTAAGCGCCAAGCGCGCGAGTGGCTGGAGAATGGGGCGATTCGCGTCAATGGTGAGCGAGTACAG
CAAGAGGGTGGGTTATCCGGCTTTGCTCTGTTTGATCGCTACTATGTGTTGCAACGTGGTAAGAAGCAGTTTGCTTTGAT
CAAATTGGAAGGCTTGGCTGAGTAA

Upstream 100 bases:

>100_bases
CGCAGCCGCGGATTAGGCTGTCTCTCTTCCTTCGGTCATCAATTACCAAGATTGTCCCTTTAGGGACATCGCTCCCTATG
TTTAAAGTTGGTTACTCTGC

Downstream 100 bases:

>100_bases
TCACAGCGATTCTACTCGGCCAAGAAAAGCAGTGGGGTTAGCCCCACTGACTTTCAAACCAACTTTCCAGAATGATCACC
GCCGATTGGCAGTCCACATT

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase 1; TyrRS 1 [H]

Number of amino acids: Translated: 434; Mature: 433

Protein sequence:

>434_residues
MPRLLGGRMNSQQLFDDLQQRGLIAQSTPLEALVERFATPQVLYCGFDPTAGSLHIGHLVPLLMLKRFQNAGHQAIALIG
GATGMIGDPSFKANERVLNSAQTVQQWVSDLSQQITQLMTSQGENCASLMAVNNADWIGKINLIDFFRDVGKHFSVNAMI
NRESVKQRLARPDQGISFTEFSYSLLQSYDFAHLNREFGCALQIGGNDQWGNIVSGIDLTRRLNGVEVCGLTLPLITKSD
GTKFGKTESGAVWLESSKTSPYRFYQFWLNSDDADVYRFLAYYTFLSTAEIEEIRQADALRAGKPQAQQILAEHVTRFVH
GEEGLAAAQRITQALFSGDVSYLSLSELAQLELDGLPCVAASRTDSLLDLLLSSGLASSKRQAREWLENGAIRVNGERVQ
QEGGLSGFALFDRYYVLQRGKKQFALIKLEGLAE

Sequences:

>Translated_434_residues
MPRLLGGRMNSQQLFDDLQQRGLIAQSTPLEALVERFATPQVLYCGFDPTAGSLHIGHLVPLLMLKRFQNAGHQAIALIG
GATGMIGDPSFKANERVLNSAQTVQQWVSDLSQQITQLMTSQGENCASLMAVNNADWIGKINLIDFFRDVGKHFSVNAMI
NRESVKQRLARPDQGISFTEFSYSLLQSYDFAHLNREFGCALQIGGNDQWGNIVSGIDLTRRLNGVEVCGLTLPLITKSD
GTKFGKTESGAVWLESSKTSPYRFYQFWLNSDDADVYRFLAYYTFLSTAEIEEIRQADALRAGKPQAQQILAEHVTRFVH
GEEGLAAAQRITQALFSGDVSYLSLSELAQLELDGLPCVAASRTDSLLDLLLSSGLASSKRQAREWLENGAIRVNGERVQ
QEGGLSGFALFDRYYVLQRGKKQFALIKLEGLAE
>Mature_433_residues
PRLLGGRMNSQQLFDDLQQRGLIAQSTPLEALVERFATPQVLYCGFDPTAGSLHIGHLVPLLMLKRFQNAGHQAIALIGG
ATGMIGDPSFKANERVLNSAQTVQQWVSDLSQQITQLMTSQGENCASLMAVNNADWIGKINLIDFFRDVGKHFSVNAMIN
RESVKQRLARPDQGISFTEFSYSLLQSYDFAHLNREFGCALQIGGNDQWGNIVSGIDLTRRLNGVEVCGLTLPLITKSDG
TKFGKTESGAVWLESSKTSPYRFYQFWLNSDDADVYRFLAYYTFLSTAEIEEIRQADALRAGKPQAQQILAEHVTRFVHG
EEGLAAAQRITQALFSGDVSYLSLSELAQLELDGLPCVAASRTDSLLDLLLSSGLASSKRQAREWLENGAIRVNGERVQQ
EGGLSGFALFDRYYVLQRGKKQFALIKLEGLAE

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=313, Percent_Identity=43.4504792332268, Blast_Score=252, Evalue=6e-67,
Organism=Escherichia coli, GI1787925, Length=425, Percent_Identity=58.5882352941177, Blast_Score=498, Evalue=1e-142,
Organism=Caenorhabditis elegans, GI25145802, Length=391, Percent_Identity=36.0613810741688, Blast_Score=222, Evalue=2e-58,
Organism=Caenorhabditis elegans, GI25145805, Length=293, Percent_Identity=34.4709897610922, Blast_Score=165, Evalue=5e-41,
Organism=Saccharomyces cerevisiae, GI6325160, Length=441, Percent_Identity=34.6938775510204, Blast_Score=257, Evalue=2e-69,
Organism=Drosophila melanogaster, GI20130383, Length=407, Percent_Identity=38.8206388206388, Blast_Score=282, Evalue=3e-76,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 48001; Mature: 47870

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: PS50889 S4 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRLLGGRMNSQQLFDDLQQRGLIAQSTPLEALVERFATPQVLYCGFDPTAGSLHIGHLV
CCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCEEEECCCCCCCCEEHHHHH
PLLMLKRFQNAGHQAIALIGGATGMIGDPSFKANERVLNSAQTVQQWVSDLSQQITQLMT
HHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SQGENCASLMAVNNADWIGKINLIDFFRDVGKHFSVNAMINRESVKQRLARPDQGISFTE
CCCCCHHHHEEECCCCCEEEHHHHHHHHHHCCCEEEEEEECHHHHHHHHCCCCCCCCHHH
FSYSLLQSYDFAHLNREFGCALQIGGNDQWGNIVSGIDLTRRLNGVEVCGLTLPLITKSD
HHHHHHHHCCHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCEEEECEEEEEEECCC
GTKFGKTESGAVWLESSKTSPYRFYQFWLNSDDADVYRFLAYYTFLSTAEIEEIRQADAL
CCCCCCCCCCEEEEECCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RAGKPQAQQILAEHVTRFVHGEEGLAAAQRITQALFSGDVSYLSLSELAQLELDGLPCVA
CCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHEEHHHHHHHHCCCCCEEE
ASRTDSLLDLLLSSGLASSKRQAREWLENGAIRVNGERVQQEGGLSGFALFDRYYVLQRG
CCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEECHHHHHHHCCCCHHHHHHHHHHHHCC
KKQFALIKLEGLAE
CCEEEEEEECCCCC
>Mature Secondary Structure 
PRLLGGRMNSQQLFDDLQQRGLIAQSTPLEALVERFATPQVLYCGFDPTAGSLHIGHLV
CCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCEEEECCCCCCCCEEHHHHH
PLLMLKRFQNAGHQAIALIGGATGMIGDPSFKANERVLNSAQTVQQWVSDLSQQITQLMT
HHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SQGENCASLMAVNNADWIGKINLIDFFRDVGKHFSVNAMINRESVKQRLARPDQGISFTE
CCCCCHHHHEEECCCCCEEEHHHHHHHHHHCCCEEEEEEECHHHHHHHHCCCCCCCCHHH
FSYSLLQSYDFAHLNREFGCALQIGGNDQWGNIVSGIDLTRRLNGVEVCGLTLPLITKSD
HHHHHHHHCCHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCEEEECEEEEEEECCC
GTKFGKTESGAVWLESSKTSPYRFYQFWLNSDDADVYRFLAYYTFLSTAEIEEIRQADAL
CCCCCCCCCCEEEEECCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RAGKPQAQQILAEHVTRFVHGEEGLAAAQRITQALFSGDVSYLSLSELAQLELDGLPCVA
CCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHEEHHHHHHHHCCCCCEEE
ASRTDSLLDLLLSSGLASSKRQAREWLENGAIRVNGERVQQEGGLSGFALFDRYYVLQRG
CCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEECHHHHHHHCCCCHHHHHHHHHHHHCC
KKQFALIKLEGLAE
CCEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]