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The map label for this gene is mshE [H]

Identifier: 227080616

GI number: 227080616

Start: 416101

End: 417828

Strand: Direct

Name: mshE [H]

Synonym: VCM66_0390

Alternate gene names: 227080616

Gene position: 416101-417828 (Clockwise)

Preceding gene: 227080615

Following gene: 227080617

Centisome position: 14.39

GC content: 50.06

Gene sequence:

>1728_bases
GTGCCAATTAATAAACTGCGTAAACGGCTTGGTGACTTGCTGGTTGAAGAGGGGATTGTGAGCGAAGCGCAGCTTGAACA
AGCGCTCAATGCCCAGAAAAATACCGGACGCCGCTTAGGGGATACCTTAATTTCGCTTGGCTTTTTAAGCGAAACCCAGT
TGCTGAACTTTTTGGCGCAGCAATTGAGTTTGCCAGTGATCGATCTGAGCCGTGCCCATGTCGATATTGATGCTGTTCCT
TTACTGCCTGAAGTTCATGCCCGCCGTTTGCGTGCGCTGGTGATAGGCCGCAGTGGTGATACGCTGCGCATAGCCATGAG
TGATCCTGCGGATTTGTTTGCTCAAGAAGCCTTGCTCAACCAGTTGCCTGATTATGGTTTTGAGTTTGTCATCGCCCCTG
AGAAGCAGTTGGTCGATGGCTTTGATCGTTACTATCGCCGCACCAAAGAAATTGTCTCGTTTGCCGAGCAATTGCATGCC
GAACACAAAACCAATGATAGTTTTGATTTTGAGATCACCGATTCAGATAGCGATGAAGTGACGGTTGTTAAACTGATCAA
CTCGCTGTTTGAAGATGCGATTCAAGTGGGAGCCTCGGATATACATATTGAGCCGGATGCCAACGTACTGCGTTTGCGCC
AACGGATCGATGGGGTATTGCATGAAACCTTGCTCCATGAAGTGAATATCGCCTCAGCCTTAGTGCTGCGCTTAAAATTA
ATGGCGAATCTGGATATTTCAGAAAAACGCCTTCCGCAAGATGGCCGCTTTAACATCCGTGTAAAAGGACAATCCGTCGA
TATCCGGATGTCAACCATGCCTATCCAGCACGGTGAATCGGTGGTGATGCGTTTGCTCAACCAATCTGCAGGGGTTAGAA
AGCTCGAAGAGTCAGGTATTCCGCCTCATTTATTGCTGCGTTTGCGCCATCAGTTGAAACGCCCTCATGGCATGATTCTG
GTGACTGGGCCTACCGGCTCGGGGAAAACCACCACCTTGTATGGCGCACTCAACGAGCTCAATACGTCCGGCAAAAAAAT
CATTACTGCGGAAGATCCGGTGGAATACCGGATTTCACGGATCAATCAGGTGCAAGTGAACCCGAAAATCAATCTCGATT
TCTCGACGTTACTGCGCACTTTCTTGCGCCAAGACCCAGATATTATTTTGGTCGGTGAGATGCGTGACCAAGAGACGGTC
GAAATTGGCCTGCGCGCTGCATTAACCGGTCACTTAGTCTTAAGTACTCTGCACACCAATGATGCCGTCGATAGCGCGCT
GCGTTTGATTGATATGGGGGCGCCCGGTTATCTGGTTGCCAGTGCGGTACGTGCCGTGGTTGCGCAGCGTTTAGTGCGAA
AAGTCTGTCCAGATTGTAGTGGACATGATGAGGTGGATGAAGCGCGTCGCCAATGGCTTGTGACGCGTTTCCCTAATCAA
GCTGCCGCCAAATTCACTCGTGGACGCGGCTGCCAGAACTGTAACTTAACCGGTTATCGCGGCCGGATCGGTGTATTTGA
AATGCTTGAGCTGGATCAGCCGATGATGGATTGCTTGCGGGCAAATGATGCTGTGGCCTTCTCTAAAGCCGCACGCAGTA
ACACAGATTATAAACCGCTACTGGCTTCGGCGATGGAGTTAGCGCTACAAGGCATCGTCAGTCTTGATGAAGTGATGTCG
CTGGGTGAAGGTGATTCCTCTGGTTTGGTTGAACCGATTTATCTGTAG

Upstream 100 bases:

>100_bases
GCCTATCAACAGGCTCTCAATAAAGTCGGATTATCCAGTCAATCACAAGCTTTTATTCATCAACGCTTGCAATTACTGGC
CAGTTTAGAGGAGACACAAA

Downstream 100 bases:

>100_bases
GAGTAGGCATGGCGACGTTTTATTATCAGGGGCGTAATGCCGATGGCAGCAAAGCCTCTGGGTTAGTCGAGGCTGCCACT
GAGGAATTAGCCGCAGAAAT

Product: MSHA biogenesis protein MshE

Products: NA

Alternate protein names: Cholera toxin secretion protein epsE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 575; Mature: 574

Protein sequence:

>575_residues
MPINKLRKRLGDLLVEEGIVSEAQLEQALNAQKNTGRRLGDTLISLGFLSETQLLNFLAQQLSLPVIDLSRAHVDIDAVP
LLPEVHARRLRALVIGRSGDTLRIAMSDPADLFAQEALLNQLPDYGFEFVIAPEKQLVDGFDRYYRRTKEIVSFAEQLHA
EHKTNDSFDFEITDSDSDEVTVVKLINSLFEDAIQVGASDIHIEPDANVLRLRQRIDGVLHETLLHEVNIASALVLRLKL
MANLDISEKRLPQDGRFNIRVKGQSVDIRMSTMPIQHGESVVMRLLNQSAGVRKLEESGIPPHLLLRLRHQLKRPHGMIL
VTGPTGSGKTTTLYGALNELNTSGKKIITAEDPVEYRISRINQVQVNPKINLDFSTLLRTFLRQDPDIILVGEMRDQETV
EIGLRAALTGHLVLSTLHTNDAVDSALRLIDMGAPGYLVASAVRAVVAQRLVRKVCPDCSGHDEVDEARRQWLVTRFPNQ
AAAKFTRGRGCQNCNLTGYRGRIGVFEMLELDQPMMDCLRANDAVAFSKAARSNTDYKPLLASAMELALQGIVSLDEVMS
LGEGDSSGLVEPIYL

Sequences:

>Translated_575_residues
MPINKLRKRLGDLLVEEGIVSEAQLEQALNAQKNTGRRLGDTLISLGFLSETQLLNFLAQQLSLPVIDLSRAHVDIDAVP
LLPEVHARRLRALVIGRSGDTLRIAMSDPADLFAQEALLNQLPDYGFEFVIAPEKQLVDGFDRYYRRTKEIVSFAEQLHA
EHKTNDSFDFEITDSDSDEVTVVKLINSLFEDAIQVGASDIHIEPDANVLRLRQRIDGVLHETLLHEVNIASALVLRLKL
MANLDISEKRLPQDGRFNIRVKGQSVDIRMSTMPIQHGESVVMRLLNQSAGVRKLEESGIPPHLLLRLRHQLKRPHGMIL
VTGPTGSGKTTTLYGALNELNTSGKKIITAEDPVEYRISRINQVQVNPKINLDFSTLLRTFLRQDPDIILVGEMRDQETV
EIGLRAALTGHLVLSTLHTNDAVDSALRLIDMGAPGYLVASAVRAVVAQRLVRKVCPDCSGHDEVDEARRQWLVTRFPNQ
AAAKFTRGRGCQNCNLTGYRGRIGVFEMLELDQPMMDCLRANDAVAFSKAARSNTDYKPLLASAMELALQGIVSLDEVMS
LGEGDSSGLVEPIYL
>Mature_574_residues
PINKLRKRLGDLLVEEGIVSEAQLEQALNAQKNTGRRLGDTLISLGFLSETQLLNFLAQQLSLPVIDLSRAHVDIDAVPL
LPEVHARRLRALVIGRSGDTLRIAMSDPADLFAQEALLNQLPDYGFEFVIAPEKQLVDGFDRYYRRTKEIVSFAEQLHAE
HKTNDSFDFEITDSDSDEVTVVKLINSLFEDAIQVGASDIHIEPDANVLRLRQRIDGVLHETLLHEVNIASALVLRLKLM
ANLDISEKRLPQDGRFNIRVKGQSVDIRMSTMPIQHGESVVMRLLNQSAGVRKLEESGIPPHLLLRLRHQLKRPHGMILV
TGPTGSGKTTTLYGALNELNTSGKKIITAEDPVEYRISRINQVQVNPKINLDFSTLLRTFLRQDPDIILVGEMRDQETVE
IGLRAALTGHLVLSTLHTNDAVDSALRLIDMGAPGYLVASAVRAVVAQRLVRKVCPDCSGHDEVDEARRQWLVTRFPNQA
AAKFTRGRGCQNCNLTGYRGRIGVFEMLELDQPMMDCLRANDAVAFSKAARSNTDYKPLLASAMELALQGIVSLDEVMSL
GEGDSSGLVEPIYL

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=384, Percent_Identity=41.9270833333333, Blast_Score=316, Evalue=3e-87,
Organism=Escherichia coli, GI1786296, Length=435, Percent_Identity=38.1609195402299, Blast_Score=298, Evalue=7e-82,
Organism=Escherichia coli, GI87082188, Length=217, Percent_Identity=39.1705069124424, Blast_Score=118, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 63845; Mature: 63714

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPINKLRKRLGDLLVEEGIVSEAQLEQALNAQKNTGRRLGDTLISLGFLSETQLLNFLAQ
CCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHCCHHHHHHHHHHHCCHHHHHHHHHHH
QLSLPVIDLSRAHVDIDAVPLLPEVHARRLRALVIGRSGDTLRIAMSDPADLFAQEALLN
HHCCCEEECCCCEECCCCCCCCHHHHHHHHHEEEEECCCCEEEEEECCCHHHHHHHHHHH
QLPDYGFEFVIAPEKQLVDGFDRYYRRTKEIVSFAEQLHAEHKTNDSFDFEITDSDSDEV
HCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCC
TVVKLINSLFEDAIQVGASDIHIEPDANVLRLRQRIDGVLHETLLHEVNIASALVLRLKL
HHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MANLDISEKRLPQDGRFNIRVKGQSVDIRMSTMPIQHGESVVMRLLNQSAGVRKLEESGI
HHCCCCCHHHCCCCCCEEEEEECCEEEEEEEECCCCCCHHHHHHHHHCCCCCHHHHHCCC
PPHLLLRLRHQLKRPHGMILVTGPTGSGKTTTLYGALNELNTSGKKIITAEDPVEYRISR
CHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEEEHHHCCCCCCEEEECCCCHHHHHHH
INQVQVNPKINLDFSTLLRTFLRQDPDIILVGEMRDQETVEIGLRAALTGHLVLSTLHTN
CCEEEECCEECCCHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCH
DAVDSALRLIDMGAPGYLVASAVRAVVAQRLVRKVCPDCSGHDEVDEARRQWLVTRFPNQ
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCH
AAAKFTRGRGCQNCNLTGYRGRIGVFEMLELDQPMMDCLRANDAVAFSKAARSNTDYKPL
HHHHHHHCCCCCCCCCCCCCCCCCHHHHHHCCHHHHHHHHCCCHHHHHHHHCCCCCCHHH
LASAMELALQGIVSLDEVMSLGEGDSSGLVEPIYL
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PINKLRKRLGDLLVEEGIVSEAQLEQALNAQKNTGRRLGDTLISLGFLSETQLLNFLAQ
CHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHCCHHHHHHHHHHHCCHHHHHHHHHHH
QLSLPVIDLSRAHVDIDAVPLLPEVHARRLRALVIGRSGDTLRIAMSDPADLFAQEALLN
HHCCCEEECCCCEECCCCCCCCHHHHHHHHHEEEEECCCCEEEEEECCCHHHHHHHHHHH
QLPDYGFEFVIAPEKQLVDGFDRYYRRTKEIVSFAEQLHAEHKTNDSFDFEITDSDSDEV
HCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCC
TVVKLINSLFEDAIQVGASDIHIEPDANVLRLRQRIDGVLHETLLHEVNIASALVLRLKL
HHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MANLDISEKRLPQDGRFNIRVKGQSVDIRMSTMPIQHGESVVMRLLNQSAGVRKLEESGI
HHCCCCCHHHCCCCCCEEEEEECCEEEEEEEECCCCCCHHHHHHHHHCCCCCHHHHHCCC
PPHLLLRLRHQLKRPHGMILVTGPTGSGKTTTLYGALNELNTSGKKIITAEDPVEYRISR
CHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEEEHHHCCCCCCEEEECCCCHHHHHHH
INQVQVNPKINLDFSTLLRTFLRQDPDIILVGEMRDQETVEIGLRAALTGHLVLSTLHTN
CCEEEECCEECCCHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCH
DAVDSALRLIDMGAPGYLVASAVRAVVAQRLVRKVCPDCSGHDEVDEARRQWLVTRFPNQ
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCH
AAAKFTRGRGCQNCNLTGYRGRIGVFEMLELDQPMMDCLRANDAVAFSKAARSNTDYKPL
HHHHHHHCCCCCCCCCCCCCCCCCHHHHHHCCHHHHHHHHCCCHHHHHHHHCCCCCCHHH
LASAMELALQGIVSLDEVMSLGEGDSSGLVEPIYL
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8423007; 10952301 [H]