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The map label for this gene is dusA

Identifier: 227080591

GI number: 227080591

Start: 379056

End: 380039

Strand: Direct

Name: dusA

Synonym: VCM66_0363

Alternate gene names: 227080591

Gene position: 379056-380039 (Clockwise)

Preceding gene: 227080588

Following gene: 227080592

Centisome position: 13.1

GC content: 50.51

Gene sequence:

>984_bases
ATGACTCACTCATGCAGACTCTCAGTTGCTCCAATGCTCGATTGGACCGACCGCCACTGCCGTTATTTCCACCGTCTGCT
CTCTGCGCAGACTCTGCTGTACACCGAAATGGTGACGACTGGCGCGATCATCCATGGTCGTGGCGATTTTCTGGCGTACA
ACCAAGAAGAGCATCCCGTAGCGCTACAGTTTGGTGGTTCCAATCCTAAGGATTTAGCGCATTGTGCCAAGCTGGCTCAA
GAGCGAGGCTATGATGAAATTAACCTCAACGTCGGCTGTCCATCCGATCGGGTACAGAATGGCCGCTTTGGCGCTTGTCT
GATGGGCGAGCCGGACTTGGTGGCGGAGTGCGTCGCCGCCATGCGTGCGGTTGTCGACATTCCGGTGACGGTCAAAACGC
GTATCGGTATTGATGATCAAGACTCGTACGAGTTTTTAACTCAGTTTATCGCCACAGTTGCGGAAAAAGGTGGCTGTGAG
CAATTTACTATCCATGCGCGTAAAGCTTGGTTAAGTGGTCTGAGCCCGAAAGAAAACCGTGAAATTCCACCACTGGATTA
CCCACGAGCGTACCAAATCAAACGTGATTTTCCGCACTTAACCATTGCAGTTAACGGTGGGGTGAAGAGCTTGGAAGAAG
CGAAACTTCATCTGCAACATCTTGATGGTGTGATGATTGGGCGTGAGGCGTATCAAAATCCGTATTTGCTGGCTGAAGTG
GATCAGCAGATTTTTGGCTTAGAAACGCCTGTGAAAAAGCGCTCACAAGTCATCCATGAAATGATGCCTTATATTGAGCG
TGAACTTTCACAAGGCACCCATCTTGGTCATATGACTCGCCATATGTTAGGACTGTTCCAAAATATGCCGGGTGCACGCC
AATGGCGACGCCATATCAGTGAGAATGCGCACAAGCCTGGAGCGGGATTGGAAGTGGTTGAACAGGCGTTGGCCAAGATC
CCTTACCAAGAGTTAGGTGTGTAA

Upstream 100 bases:

>100_bases
AAGGCATGGCGCTCACGCTAAGTTGCGATTAAAATGCCGGACTTCGATTTTTTCGCCACCGTGCGGTGGTTGTTTTTGAC
TGAAATGGGGACGTTCACCT

Downstream 100 bases:

>100_bases
AAAACACCAAACATTAGCGAAAATGCTTACTGTTTTTTGATAAGCCAAGTGAGATGACGTCGCTTGGCTTATTTTTTTCT
ATATTAATCAAATGGTTGGC

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MTHSCRLSVAPMLDWTDRHCRYFHRLLSAQTLLYTEMVTTGAIIHGRGDFLAYNQEEHPVALQFGGSNPKDLAHCAKLAQ
ERGYDEINLNVGCPSDRVQNGRFGACLMGEPDLVAECVAAMRAVVDIPVTVKTRIGIDDQDSYEFLTQFIATVAEKGGCE
QFTIHARKAWLSGLSPKENREIPPLDYPRAYQIKRDFPHLTIAVNGGVKSLEEAKLHLQHLDGVMIGREAYQNPYLLAEV
DQQIFGLETPVKKRSQVIHEMMPYIERELSQGTHLGHMTRHMLGLFQNMPGARQWRRHISENAHKPGAGLEVVEQALAKI
PYQELGV

Sequences:

>Translated_327_residues
MTHSCRLSVAPMLDWTDRHCRYFHRLLSAQTLLYTEMVTTGAIIHGRGDFLAYNQEEHPVALQFGGSNPKDLAHCAKLAQ
ERGYDEINLNVGCPSDRVQNGRFGACLMGEPDLVAECVAAMRAVVDIPVTVKTRIGIDDQDSYEFLTQFIATVAEKGGCE
QFTIHARKAWLSGLSPKENREIPPLDYPRAYQIKRDFPHLTIAVNGGVKSLEEAKLHLQHLDGVMIGREAYQNPYLLAEV
DQQIFGLETPVKKRSQVIHEMMPYIERELSQGTHLGHMTRHMLGLFQNMPGARQWRRHISENAHKPGAGLEVVEQALAKI
PYQELGV
>Mature_326_residues
THSCRLSVAPMLDWTDRHCRYFHRLLSAQTLLYTEMVTTGAIIHGRGDFLAYNQEEHPVALQFGGSNPKDLAHCAKLAQE
RGYDEINLNVGCPSDRVQNGRFGACLMGEPDLVAECVAAMRAVVDIPVTVKTRIGIDDQDSYEFLTQFIATVAEKGGCEQ
FTIHARKAWLSGLSPKENREIPPLDYPRAYQIKRDFPHLTIAVNGGVKSLEEAKLHLQHLDGVMIGREAYQNPYLLAEVD
QQIFGLETPVKKRSQVIHEMMPYIERELSQGTHLGHMTRHMLGLFQNMPGARQWRRHISENAHKPGAGLEVVEQALAKIP
YQELGV

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusA subfamily

Homologues:

Organism=Homo sapiens, GI31742496, Length=307, Percent_Identity=27.3615635179153, Blast_Score=85, Evalue=7e-17,
Organism=Homo sapiens, GI40807366, Length=243, Percent_Identity=27.1604938271605, Blast_Score=76, Evalue=5e-14,
Organism=Homo sapiens, GI239788483, Length=213, Percent_Identity=26.2910798122066, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI239788462, Length=213, Percent_Identity=26.2910798122066, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI145693211, Length=314, Percent_Identity=77.0700636942675, Blast_Score=515, Evalue=1e-147,
Organism=Escherichia coli, GI1789660, Length=240, Percent_Identity=28.75, Blast_Score=85, Evalue=8e-18,
Organism=Escherichia coli, GI1788462, Length=226, Percent_Identity=26.9911504424779, Blast_Score=72, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI25144369, Length=213, Percent_Identity=27.6995305164319, Blast_Score=77, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17507177, Length=168, Percent_Identity=27.9761904761905, Blast_Score=76, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6323560, Length=293, Percent_Identity=23.8907849829352, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24580595, Length=296, Percent_Identity=26.6891891891892, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI19920448, Length=296, Percent_Identity=26.6891891891892, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI19921524, Length=235, Percent_Identity=27.2340425531915, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DUSA_VIBCH (Q9KUX9)

Other databases:

- EMBL:   AE003852
- PIR:   G82330
- RefSeq:   NP_230033.1
- ProteinModelPortal:   Q9KUX9
- SMR:   Q9KUX9
- GeneID:   2615022
- GenomeReviews:   AE003852_GR
- KEGG:   vch:VC0379
- TIGR:   VC_0379
- HOGENOM:   HBG630545
- OMA:   RIGIDEQ
- ProtClustDB:   PRK11815
- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR11082
- PIRSF:   PIRSF006621
- TIGRFAMs:   TIGR00742

Pfam domain/function: PF01207 Dus

EC number: 1.-.-.-

Molecular weight: Translated: 36792; Mature: 36660

Theoretical pI: Translated: 6.83; Mature: 6.83

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHSCRLSVAPMLDWTDRHCRYFHRLLSAQTLLYTEMVTTGAIIHGRGDFLAYNQEEHPV
CCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCEEEECCCCCCE
ALQFGGSNPKDLAHCAKLAQERGYDEINLNVGCPSDRVQNGRFGACLMGEPDLVAECVAA
EEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHCCCCCCEEECCCHHHHHHHHHH
MRAVVDIPVTVKTRIGIDDQDSYEFLTQFIATVAEKGGCEQFTIHARKAWLSGLSPKENR
HHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHCCCCCCCCC
EIPPLDYPRAYQIKRDFPHLTIAVNGGVKSLEEAKLHLQHLDGVMIGREAYQNPYLLAEV
CCCCCCCCCCCCEECCCCEEEEEECCCHHHHHHHHHHHHHHCCHHCCHHHHCCCEEEEHH
DQQIFGLETPVKKRSQVIHEMMPYIERELSQGTHLGHMTRHMLGLFQNMPGARQWRRHIS
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHH
ENAHKPGAGLEVVEQALAKIPYQELGV
HCCCCCCCCHHHHHHHHHHCCHHHHCC
>Mature Secondary Structure 
THSCRLSVAPMLDWTDRHCRYFHRLLSAQTLLYTEMVTTGAIIHGRGDFLAYNQEEHPV
CCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCEEEECCCCCCE
ALQFGGSNPKDLAHCAKLAQERGYDEINLNVGCPSDRVQNGRFGACLMGEPDLVAECVAA
EEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHCCCCCCEEECCCHHHHHHHHHH
MRAVVDIPVTVKTRIGIDDQDSYEFLTQFIATVAEKGGCEQFTIHARKAWLSGLSPKENR
HHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHCCCCCCCCC
EIPPLDYPRAYQIKRDFPHLTIAVNGGVKSLEEAKLHLQHLDGVMIGREAYQNPYLLAEV
CCCCCCCCCCCCEECCCCEEEEEECCCHHHHHHHHHHHHHHCCHHCCHHHHCCCEEEEHH
DQQIFGLETPVKKRSQVIHEMMPYIERELSQGTHLGHMTRHMLGLFQNMPGARQWRRHIS
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHH
ENAHKPGAGLEVVEQALAKIPYQELGV
HCCCCCCCCHHHHHHHHHHCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301