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The map label for this gene is alr [H]

Identifier: 227080584

GI number: 227080584

Start: 372699

End: 373826

Strand: Direct

Name: alr [H]

Synonym: VCM66_0356

Alternate gene names: 227080584

Gene position: 372699-373826 (Clockwise)

Preceding gene: 227080583

Following gene: 227080586

Centisome position: 12.88

GC content: 52.39

Gene sequence:

>1128_bases
ATGCTGGACCTGCATTTGACGATGAGTAATTCGATGAACTATATGAAGGCGGCCACCGCCTACATCAATTTAGAGGCGTT
GCAGCACAACTTGCAGCGCGTTAAGCAGCAAGCGCCAGAGAGTAAAATCATGGCGGTGGTGAAAGCCAATGGTTATGGAC
ACGGGCTGCGCCATATCGCACGTCACGCCCTTGGGGCGGATGCCTTTGGCGTGGCGCGCATTGAAGAGGCTTTACAGCTG
CGCGCCAGTGGCGTAGTGAAACCCATTTTGTTGCTTGAGGGATTTTATTCTCCCGGTGATTTGCCAGTATTGGTTACCAA
CAACATCCAAACCGTCGTACATTGTGAAGAGCAGTTACAAGCGCTGGAGCAAGCTCAGCTAGAGACTCCGGTCATGGTAT
GGCTGAAAGTCGACAGCGGGATGCATCGGCTAGGGGTTCGCCCTGAACAATATCAAGATTTCGTGGCTCGTTTGCACCAG
TGTGAGAATGTGGCTAAACCACTGCGTTACATGAGCCATTTCGGCTGTGCCGATGAGTTAGATAAATCGACGACGGTAGA
GCAAACAGAACTGTTTTTATCCCTGACGCAAGGCTGTCAGGGTGAGCGTTCGCTGGCCGCTTCTGCCGGATTATTAGCTT
GGCCGCAAAGCCAGCTTGAATGGGTAAGGCCGGGAATTATTATGTACGGCGTTTCTCCCTTTGTGGAAAAGAGCGCGGTT
CAGTTAGGTTACCAGCCAGTGATGACCTTAAAGTCGCACTTGATCGCTGTCCGCGAAGTGAAAGCGGGGGAGAGTGTCGG
TTATGGCGGTACTTGGACCAGCCAGCGAGATACCAAAATTGGTGTGATTGCGATTGGTTATGGTGATGGCTATCCACGCA
CTGCGCCGAATGGGACGCCAGTGGTTGTCAATGGGCGTCGGGTGCCGATTGCTGGGCGCGTATCTATGGATATGTTGACG
GTAGATTTAGGGCCGGATGCTTGCGATCGCGTCGGTGATGAAGCCATGCTGTGGGGTAATGAGCTGCCCGTAGAAGAAGT
GGCCGCACATATTGGTACGATAGGGTATGAGTTAGTGACGAAACTCACCTCGCGAGTGGAAATGTCTTATTATGGAGCAG
GCGTTTAG

Upstream 100 bases:

>100_bases
CTAAAAGGCACGGCGGAAATCATTATTGGTAAGCAACGTAACGGCCCGATTGGTTCGGTGCGTTTGACTTTCCAAGGTCA
CTATTCTCGTTTCGACAACT

Downstream 100 bases:

>100_bases
CCTGCTTTGTGTTGTCCGTGCATGACCACCGCTTATCGCTGTGGCCATGCATGTCACTCTCTTCTCTTGCACTCTTTGGG
CGTTACTCGCCTTGAATGGT

Product: alanine racemase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MLDLHLTMSNSMNYMKAATAYINLEALQHNLQRVKQQAPESKIMAVVKANGYGHGLRHIARHALGADAFGVARIEEALQL
RASGVVKPILLLEGFYSPGDLPVLVTNNIQTVVHCEEQLQALEQAQLETPVMVWLKVDSGMHRLGVRPEQYQDFVARLHQ
CENVAKPLRYMSHFGCADELDKSTTVEQTELFLSLTQGCQGERSLAASAGLLAWPQSQLEWVRPGIIMYGVSPFVEKSAV
QLGYQPVMTLKSHLIAVREVKAGESVGYGGTWTSQRDTKIGVIAIGYGDGYPRTAPNGTPVVVNGRRVPIAGRVSMDMLT
VDLGPDACDRVGDEAMLWGNELPVEEVAAHIGTIGYELVTKLTSRVEMSYYGAGV

Sequences:

>Translated_375_residues
MLDLHLTMSNSMNYMKAATAYINLEALQHNLQRVKQQAPESKIMAVVKANGYGHGLRHIARHALGADAFGVARIEEALQL
RASGVVKPILLLEGFYSPGDLPVLVTNNIQTVVHCEEQLQALEQAQLETPVMVWLKVDSGMHRLGVRPEQYQDFVARLHQ
CENVAKPLRYMSHFGCADELDKSTTVEQTELFLSLTQGCQGERSLAASAGLLAWPQSQLEWVRPGIIMYGVSPFVEKSAV
QLGYQPVMTLKSHLIAVREVKAGESVGYGGTWTSQRDTKIGVIAIGYGDGYPRTAPNGTPVVVNGRRVPIAGRVSMDMLT
VDLGPDACDRVGDEAMLWGNELPVEEVAAHIGTIGYELVTKLTSRVEMSYYGAGV
>Mature_375_residues
MLDLHLTMSNSMNYMKAATAYINLEALQHNLQRVKQQAPESKIMAVVKANGYGHGLRHIARHALGADAFGVARIEEALQL
RASGVVKPILLLEGFYSPGDLPVLVTNNIQTVVHCEEQLQALEQAQLETPVMVWLKVDSGMHRLGVRPEQYQDFVARLHQ
CENVAKPLRYMSHFGCADELDKSTTVEQTELFLSLTQGCQGERSLAASAGLLAWPQSQLEWVRPGIIMYGVSPFVEKSAV
QLGYQPVMTLKSHLIAVREVKAGESVGYGGTWTSQRDTKIGVIAIGYGDGYPRTAPNGTPVVVNGRRVPIAGRVSMDMLT
VDLGPDACDRVGDEAMLWGNELPVEEVAAHIGTIGYELVTKLTSRVEMSYYGAGV

Specific function: Provides the D-alanine required for cell wall biosynthesis [H]

COG id: COG0787

COG function: function code M; Alanine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alanine racemase family [H]

Homologues:

Organism=Escherichia coli, GI1790487, Length=357, Percent_Identity=60.2240896358543, Blast_Score=448, Evalue=1e-127,
Organism=Escherichia coli, GI1787439, Length=349, Percent_Identity=43.5530085959885, Blast_Score=281, Evalue=4e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000821
- InterPro:   IPR009006
- InterPro:   IPR011079
- InterPro:   IPR001608
- InterPro:   IPR020622 [H]

Pfam domain/function: PF00842 Ala_racemase_C; PF01168 Ala_racemase_N [H]

EC number: =5.1.1.1 [H]

Molecular weight: Translated: 41002; Mature: 41002

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS00395 ALANINE_RACEMASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDLHLTMSNSMNYMKAATAYINLEALQHNLQRVKQQAPESKIMAVVKANGYGHGLRHIA
CEEEEEEECCCCHHHHHHHHHEEHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHH
RHALGADAFGVARIEEALQLRASGVVKPILLLEGFYSPGDLPVLVTNNIQTVVHCEEQLQ
HHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCEEEEEECCCEEEEEHHHHHH
ALEQAQLETPVMVWLKVDSGMHRLGVRPEQYQDFVARLHQCENVAKPLRYMSHFGCADEL
HHHHHHCCCCEEEEEEECCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHC
DKSTTVEQTELFLSLTQGCQGERSLAASAGLLAWPQSQLEWVRPGIIMYGVSPFVEKSAV
CCCCCHHHHHHHHHHHHCCCCCHHHHHCCCCEECCHHHHHHHCCCEEEECCCHHHHHHHH
QLGYQPVMTLKSHLIAVREVKAGESVGYGGTWTSQRDTKIGVIAIGYGDGYPRTAPNGTP
HHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCE
VVVNGRRVPIAGRVSMDMLTVDLGPDACDRVGDEAMLWGNELPVEEVAAHIGTIGYELVT
EEECCEEECEECCCEEEEEEEECCCCHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHH
KLTSRVEMSYYGAGV
HHHHHHHHHHCCCCC
>Mature Secondary Structure
MLDLHLTMSNSMNYMKAATAYINLEALQHNLQRVKQQAPESKIMAVVKANGYGHGLRHIA
CEEEEEEECCCCHHHHHHHHHEEHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHH
RHALGADAFGVARIEEALQLRASGVVKPILLLEGFYSPGDLPVLVTNNIQTVVHCEEQLQ
HHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCEEEEEECCCEEEEEHHHHHH
ALEQAQLETPVMVWLKVDSGMHRLGVRPEQYQDFVARLHQCENVAKPLRYMSHFGCADEL
HHHHHHCCCCEEEEEEECCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHC
DKSTTVEQTELFLSLTQGCQGERSLAASAGLLAWPQSQLEWVRPGIIMYGVSPFVEKSAV
CCCCCHHHHHHHHHHHHCCCCCHHHHHCCCCEECCHHHHHHHCCCEEEECCCHHHHHHHH
QLGYQPVMTLKSHLIAVREVKAGESVGYGGTWTSQRDTKIGVIAIGYGDGYPRTAPNGTP
HHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCE
VVVNGRRVPIAGRVSMDMLTVDLGPDACDRVGDEAMLWGNELPVEEVAAHIGTIGYELVT
EEECCEEECEECCCEEEEEEEECCCCHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHH
KLTSRVEMSYYGAGV
HHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]