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The map label for this gene is dnaB [H]

Identifier: 227080583

GI number: 227080583

Start: 371321

End: 372727

Strand: Direct

Name: dnaB [H]

Synonym: VCM66_0355

Alternate gene names: 227080583

Gene position: 371321-372727 (Clockwise)

Preceding gene: 227080581

Following gene: 227080584

Centisome position: 12.84

GC content: 50.46

Gene sequence:

>1407_bases
ATGGCAGATCCACGTACTGATAATCGAAACCGTAAAATTCCCGACGCACAAGTCGATGCAATTAAAGTCCCACCGCATTC
ATTAGAAGCTGAGCAATCCGTCATTGGTGGCTTGCTGCTGGATAATGAGCGCTGGGATACGGTCTCAGAACACGTGATGA
CTCAGGACTTCTACAGCCGCCCGCATCGTTTGATTTTTGACGGGGTAAAATCGATTTTGGAGGCGGGTAAACCGCTCGAT
CTCATTACCTTGTCCGAATATCTTGAGCAGCGTGAGCAGTTGGAAGATGTCGGTGGCTTTGCCTATTTGGCCGATTTGGC
CAAAAACACGCCCAGTGCTGCCAACATTAATGCCTATGCGGAGATTGTGGCCGAACGGGCTTTGGTGCGTAATCTGATTG
GTGTTGCCAATGAGATTGCCGATGCGGGATACGATCCACAAGGGCGCAACGCTGAAGATTTGCTGGATCTGGCGGAAAGT
AAAGTTTTTGCGATTGCCGAAGCGCGTACCAGTGAAAATGAAGGCCCGAAAAACGTGGATAGCATTCTAGAGCGCACGCT
AGAGCGGATTGAGCTGCTCTACAAAACGCCGCAGGATGGGGTCACTGGAGTGAACACGGGCTTTACCGACCTGAATAAAA
AGACGGCTGGTTTGCAAGGCTCGGATCTGATCATCGTCGCAGCGCGTCCTTCGATGGGTAAAACCACTTTTGCGATGAAC
CTGTGTGAAAACGCCGCGATGGAGCAAGATAAACCTGTGCTGATCTTCTCGCTGGAGATGCCCGCCGAACAGATCATGAT
GCGTATGCTGGCCTCGTTATCACGTGTCGACCAAACCAAAATTCGTACCGGTCAACTGGATGATGAAGATTGGGCGCGTA
TCTCTTCCACCATGGGGATTCTCATGGAGAAGAAGAACATGTACATCGATGATAGCTCAGGCCTAACCCCCACCGAAGTT
CGCTCGCGTGCCCGACGCATTGCTCGTGAACACGGAGGCTTGTCGCTCATCATGGTCGACTATTTACAGCTGATGCGTGT
ACCGGCATTGACCGATAACCGTACACTGGAAATTGCTGAAATTTCTCGCTCACTCAAAGCGTTAGCTAAAGAATTGAATG
TGCCCGTGGTTGCGCTTTCGCAGTTGAACCGTTCTTTGGAGCAGCGTGCGGATAAGCGTCCAGTCAACTCGGATCTGCGT
GAATCGGGTTCGATTGAGCAGGATGCTGACTTGATCATGTTTATCTATCGAGATGAGGTGTATCACCCAGATAGTCCGCT
AAAAGGCACGGCGGAAATCATTATTGGTAAGCAACGTAACGGCCCGATTGGTTCGGTGCGTTTGACTTTCCAAGGTCACT
ATTCTCGTTTCGACAACTATGCTGGACCTGCATTTGACGATGAGTAA

Upstream 100 bases:

>100_bases
CAATTCTGGTTAGGCGGCGCACAAACTTTCGTTGCTTTAATTACAAATGACGGATTCTCGTTATAATGACGGACATTCAA
ACGTTACTTAAAGCGAAGTC

Downstream 100 bases:

>100_bases
TTCGATGAACTATATGAAGGCGGCCACCGCCTACATCAATTTAGAGGCGTTGCAGCACAACTTGCAGCGCGTTAAGCAGC
AAGCGCCAGAGAGTAAAATC

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 468; Mature: 467

Protein sequence:

>468_residues
MADPRTDNRNRKIPDAQVDAIKVPPHSLEAEQSVIGGLLLDNERWDTVSEHVMTQDFYSRPHRLIFDGVKSILEAGKPLD
LITLSEYLEQREQLEDVGGFAYLADLAKNTPSAANINAYAEIVAERALVRNLIGVANEIADAGYDPQGRNAEDLLDLAES
KVFAIAEARTSENEGPKNVDSILERTLERIELLYKTPQDGVTGVNTGFTDLNKKTAGLQGSDLIIVAARPSMGKTTFAMN
LCENAAMEQDKPVLIFSLEMPAEQIMMRMLASLSRVDQTKIRTGQLDDEDWARISSTMGILMEKKNMYIDDSSGLTPTEV
RSRARRIAREHGGLSLIMVDYLQLMRVPALTDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQRADKRPVNSDLR
ESGSIEQDADLIMFIYRDEVYHPDSPLKGTAEIIIGKQRNGPIGSVRLTFQGHYSRFDNYAGPAFDDE

Sequences:

>Translated_468_residues
MADPRTDNRNRKIPDAQVDAIKVPPHSLEAEQSVIGGLLLDNERWDTVSEHVMTQDFYSRPHRLIFDGVKSILEAGKPLD
LITLSEYLEQREQLEDVGGFAYLADLAKNTPSAANINAYAEIVAERALVRNLIGVANEIADAGYDPQGRNAEDLLDLAES
KVFAIAEARTSENEGPKNVDSILERTLERIELLYKTPQDGVTGVNTGFTDLNKKTAGLQGSDLIIVAARPSMGKTTFAMN
LCENAAMEQDKPVLIFSLEMPAEQIMMRMLASLSRVDQTKIRTGQLDDEDWARISSTMGILMEKKNMYIDDSSGLTPTEV
RSRARRIAREHGGLSLIMVDYLQLMRVPALTDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQRADKRPVNSDLR
ESGSIEQDADLIMFIYRDEVYHPDSPLKGTAEIIIGKQRNGPIGSVRLTFQGHYSRFDNYAGPAFDDE
>Mature_467_residues
ADPRTDNRNRKIPDAQVDAIKVPPHSLEAEQSVIGGLLLDNERWDTVSEHVMTQDFYSRPHRLIFDGVKSILEAGKPLDL
ITLSEYLEQREQLEDVGGFAYLADLAKNTPSAANINAYAEIVAERALVRNLIGVANEIADAGYDPQGRNAEDLLDLAESK
VFAIAEARTSENEGPKNVDSILERTLERIELLYKTPQDGVTGVNTGFTDLNKKTAGLQGSDLIIVAARPSMGKTTFAMNL
CENAAMEQDKPVLIFSLEMPAEQIMMRMLASLSRVDQTKIRTGQLDDEDWARISSTMGILMEKKNMYIDDSSGLTPTEVR
SRARRIAREHGGLSLIMVDYLQLMRVPALTDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQRADKRPVNSDLRE
SGSIEQDADLIMFIYRDEVYHPDSPLKGTAEIIIGKQRNGPIGSVRLTFQGHYSRFDNYAGPAFDDE

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=471, Percent_Identity=78.1316348195329, Blast_Score=754, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 52050; Mature: 51918

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADPRTDNRNRKIPDAQVDAIKVPPHSLEAEQSVIGGLLLDNERWDTVSEHVMTQDFYSR
CCCCCCCCCCCCCCCCCCCEEECCCCCCCHHHHHHCCEEECCCCCHHHHHHHHHHHHHHC
PHRLIFDGVKSILEAGKPLDLITLSEYLEQREQLEDVGGFAYLADLAKNTPSAANINAYA
CHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCHHHHH
EIVAERALVRNLIGVANEIADAGYDPQGRNAEDLLDLAESKVFAIAEARTSENEGPKNVD
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHEEEEEHHHCCCCCCCCHHH
SILERTLERIELLYKTPQDGVTGVNTGFTDLNKKTAGLQGSDLIIVAARPSMGKTTFAMN
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHCCCCCCCEEEEEECCCCCCHHHHHH
LCENAAMEQDKPVLIFSLEMPAEQIMMRMLASLSRVDQTKIRTGQLDDEDWARISSTMGI
HHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
LMEKKNMYIDDSSGLTPTEVRSRARRIAREHGGLSLIMVDYLQLMRVPALTDNRTLEIAE
HEECCCCEEECCCCCCHHHHHHHHHHHHHHHCCEEEHHHHHHHHHHCCCCCCCCEEHHHH
ISRSLKALAKELNVPVVALSQLNRSLEQRADKRPVNSDLRESGSIEQDADLIMFIYRDEV
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCEEEEEEECCC
YHPDSPLKGTAEIIIGKQRNGPIGSVRLTFQGHYSRFDNYAGPAFDDE
CCCCCCCCCCEEEEEECCCCCCCCEEEEEEECCHHHHCCCCCCCCCCC
>Mature Secondary Structure 
ADPRTDNRNRKIPDAQVDAIKVPPHSLEAEQSVIGGLLLDNERWDTVSEHVMTQDFYSR
CCCCCCCCCCCCCCCCCCEEECCCCCCCHHHHHHCCEEECCCCCHHHHHHHHHHHHHHC
PHRLIFDGVKSILEAGKPLDLITLSEYLEQREQLEDVGGFAYLADLAKNTPSAANINAYA
CHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCHHHHH
EIVAERALVRNLIGVANEIADAGYDPQGRNAEDLLDLAESKVFAIAEARTSENEGPKNVD
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHEEEEEHHHCCCCCCCCHHH
SILERTLERIELLYKTPQDGVTGVNTGFTDLNKKTAGLQGSDLIIVAARPSMGKTTFAMN
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHCCCCCCCEEEEEECCCCCCHHHHHH
LCENAAMEQDKPVLIFSLEMPAEQIMMRMLASLSRVDQTKIRTGQLDDEDWARISSTMGI
HHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
LMEKKNMYIDDSSGLTPTEVRSRARRIAREHGGLSLIMVDYLQLMRVPALTDNRTLEIAE
HEECCCCEEECCCCCCHHHHHHHHHHHHHHHCCEEEHHHHHHHHHHCCCCCCCCEEHHHH
ISRSLKALAKELNVPVVALSQLNRSLEQRADKRPVNSDLRESGSIEQDADLIMFIYRDEV
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCEEEEEEECCC
YHPDSPLKGTAEIIIGKQRNGPIGSVRLTFQGHYSRFDNYAGPAFDDE
CCCCCCCCCCEEEEEECCCCCCCCEEEEEEECCHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]