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The map label for this gene is miaA

Identifier: 227080558

GI number: 227080558

Start: 353757

End: 354704

Strand: Direct

Name: miaA

Synonym: VCM66_0330

Alternate gene names: 227080558

Gene position: 353757-354704 (Clockwise)

Preceding gene: 227080557

Following gene: 227080559

Centisome position: 12.23

GC content: 48.84

Gene sequence:

>948_bases
ATGACTCAAAAGTTACCTCTCGCCCTCTTCTTAATGGGCCCAACGGCCTCTGGCAAAACGGATTTAGCCATTCGCTTACG
TCAGAAATATCCGGTGGAGATCATCAGCGTCGACTCGGCACTGATTTATCGAGGGATGGACATAGGCACCGCAAAACCGG
ATGCTCAAGAGCTAGCACTCGCACCGCACCGTTTGATCGATATTCTCGATCCGAGTGAAGCGTATTCGGCTGCCGATTTT
CGTCGTGATGCATTAAAAGAGATGGCGGATATCGTCGCTCAGGGCAAAATCCCACTGTTGGTGGGAGGCACAATGCTCTA
CTTTAAAGCCTTACTCGAAGGGTTATCACCACTACCTGCCGCGGATCCCGTGATTCGCCAGCAGATCGAACTGGAAGCTG
AAAAGCTTGGCTGGCAGGCGTTACACGATCAGTTACAACAGATTGATCCCGTCTCAGCACAGCGAATTCATCCCAATGAT
CCACAAAGATTGTCGCGGGCATTGGAAGTTTATCGAATTTCAGGTAAAACTCTTACTGAACTGACACAAACCAAAGGGGA
AGCAATTCCTTATCGGGTGCTGCAGTTTGCAATTGCTCCCAAGGAAAGGGCAGAACTCCATCGTCGGATTGAACTTCGCT
TTGAGAAGATGGTCGAATCAGGGTTTGAAGAAGAGGTCAAAGCATTATATGCCAGAGACGATCTTCATCCGGATCTTCCT
TCGATTCGCTGCGTCGGTTACCGACAGATGTGGGATTACTTAGACGGTCATGGAACCTTAGATGAAGCGATTTATCGCGG
CATCTGTGCCACCCGTCAATTGGCCAAGCGACAGATCACCTGGTTACGCAGCTGGGATGATTTAACTTGGTTAGATAGCG
AAAACGTTGATCAAGCCGTTGAAACGCTCTCGAATGCGATAGCTTCCAACGAAATAAGCTGTGTATAA

Upstream 100 bases:

>100_bases
TCGCAGAGCTGGAGCAGCTCTGGCAAGGCAACTTGCCTTTGCAAGACCCGCATTTTATTACTTTGGTGGATTTTTCCGCC
TCAATTACAGCATTACACTC

Downstream 100 bases:

>100_bases
TGTGATCGCTTTTGCGTGATGTACCCCGGAAAGGATCCGCTATTGGTACTCTGTTGAGCATCGGTAGCAACGGGGATTTT
GATTCGTTTAGCTCAGATGC

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 315; Mature: 314

Protein sequence:

>315_residues
MTQKLPLALFLMGPTASGKTDLAIRLRQKYPVEIISVDSALIYRGMDIGTAKPDAQELALAPHRLIDILDPSEAYSAADF
RRDALKEMADIVAQGKIPLLVGGTMLYFKALLEGLSPLPAADPVIRQQIELEAEKLGWQALHDQLQQIDPVSAQRIHPND
PQRLSRALEVYRISGKTLTELTQTKGEAIPYRVLQFAIAPKERAELHRRIELRFEKMVESGFEEEVKALYARDDLHPDLP
SIRCVGYRQMWDYLDGHGTLDEAIYRGICATRQLAKRQITWLRSWDDLTWLDSENVDQAVETLSNAIASNEISCV

Sequences:

>Translated_315_residues
MTQKLPLALFLMGPTASGKTDLAIRLRQKYPVEIISVDSALIYRGMDIGTAKPDAQELALAPHRLIDILDPSEAYSAADF
RRDALKEMADIVAQGKIPLLVGGTMLYFKALLEGLSPLPAADPVIRQQIELEAEKLGWQALHDQLQQIDPVSAQRIHPND
PQRLSRALEVYRISGKTLTELTQTKGEAIPYRVLQFAIAPKERAELHRRIELRFEKMVESGFEEEVKALYARDDLHPDLP
SIRCVGYRQMWDYLDGHGTLDEAIYRGICATRQLAKRQITWLRSWDDLTWLDSENVDQAVETLSNAIASNEISCV
>Mature_314_residues
TQKLPLALFLMGPTASGKTDLAIRLRQKYPVEIISVDSALIYRGMDIGTAKPDAQELALAPHRLIDILDPSEAYSAADFR
RDALKEMADIVAQGKIPLLVGGTMLYFKALLEGLSPLPAADPVIRQQIELEAEKLGWQALHDQLQQIDPVSAQRIHPNDP
QRLSRALEVYRISGKTLTELTQTKGEAIPYRVLQFAIAPKERAELHRRIELRFEKMVESGFEEEVKALYARDDLHPDLPS
IRCVGYRQMWDYLDGHGTLDEAIYRGICATRQLAKRQITWLRSWDDLTWLDSENVDQAVETLSNAIASNEISCV

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=319, Percent_Identity=30.4075235109718, Blast_Score=129, Evalue=5e-30,
Organism=Escherichia coli, GI1790613, Length=296, Percent_Identity=73.9864864864865, Blast_Score=460, Evalue=1e-131,
Organism=Caenorhabditis elegans, GI25144712, Length=299, Percent_Identity=30.7692307692308, Blast_Score=117, Evalue=8e-27,
Organism=Saccharomyces cerevisiae, GI6324848, Length=288, Percent_Identity=29.1666666666667, Blast_Score=130, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24649830, Length=186, Percent_Identity=35.4838709677419, Blast_Score=116, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_VIBC3 (A5F3L6)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F3L6
- SMR:   A5F3L6
- STRING:   A5F3L6
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A2757
- eggNOG:   COG0324
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- ProtClustDB:   PRK00091
- BioCyc:   VCHO345073:VC0395_A2757-MONOMER
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 35516; Mature: 35385

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQKLPLALFLMGPTASGKTDLAIRLRQKYPVEIISVDSALIYRGMDIGTAKPDAQELAL
CCCCCCEEEEEECCCCCCCCCEEEEECCCCCEEEEECCHHHHHCCCCCCCCCCCHHHHHH
APHRLIDILDPSEAYSAADFRRDALKEMADIVAQGKIPLLVGGTMLYFKALLEGLSPLPA
CHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHCCCCCCC
ADPVIRQQIELEAEKLGWQALHDQLQQIDPVSAQRIHPNDPQRLSRALEVYRISGKTLTE
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHH
LTQTKGEAIPYRVLQFAIAPKERAELHRRIELRFEKMVESGFEEEVKALYARDDLHPDLP
HHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCC
SIRCVGYRQMWDYLDGHGTLDEAIYRGICATRQLAKRQITWLRSWDDLTWLDSENVDQAV
CEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
ETLSNAIASNEISCV
HHHHHHHHCCCCCCC
>Mature Secondary Structure 
TQKLPLALFLMGPTASGKTDLAIRLRQKYPVEIISVDSALIYRGMDIGTAKPDAQELAL
CCCCCEEEEEECCCCCCCCCEEEEECCCCCEEEEECCHHHHHCCCCCCCCCCCHHHHHH
APHRLIDILDPSEAYSAADFRRDALKEMADIVAQGKIPLLVGGTMLYFKALLEGLSPLPA
CHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHCCCCCCC
ADPVIRQQIELEAEKLGWQALHDQLQQIDPVSAQRIHPNDPQRLSRALEVYRISGKTLTE
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHH
LTQTKGEAIPYRVLQFAIAPKERAELHRRIELRFEKMVESGFEEEVKALYARDDLHPDLP
HHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCC
SIRCVGYRQMWDYLDGHGTLDEAIYRGICATRQLAKRQITWLRSWDDLTWLDSENVDQAV
CEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
ETLSNAIASNEISCV
HHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA