Click here to switch to the map view.

The map label for this gene is amiB [H]

Identifier: 227080556

GI number: 227080556

Start: 350047

End: 351792

Strand: Direct

Name: amiB [H]

Synonym: VCM66_0328

Alternate gene names: 227080556

Gene position: 350047-351792 (Clockwise)

Preceding gene: 227080555

Following gene: 227080557

Centisome position: 12.1

GC content: 47.48

Gene sequence:

>1746_bases
ATGCGACCTTTTGAGTCAGTTGGAGTTATGTTGAACGTTAGCCGTTTTGTATTCTTACTCTTTGGATTCGTTTTACTCGC
GATCGCTCCTCAAACTTGGGCGAACGTTTTGGAAGGCGTGCGCGTGTGGCCTTCTCCTGATGAGACACGAGTGGTATTAG
ATGTCAAATCCGAGGTGGATTACAGCTACTTTACCCTCAGCTCTCCTGAGCGCTTGGTGGTTGACCTCAAACAGAGCACC
TCTCGCGCTAAGTTACCGGTTAATGTCACTGAAAGTGGCATTTTATCGAAAGTTCGCGCTTCATCTCCCCCTGAAAAAAG
TACATTTCGTTTGGTGTTTGAGCTCAAACAAAAAACAACGCCAACTTTATTTAAATTGGCTCCGACACCCGGTGGTCAAT
ATGGCCATCGTTTAGTCATTGATATGCCACATGGCAAAGTGAGTGAGAGCTCTTCCGCCAGCACGCCGAGTTCACCCGCT
CAAGTCAGTAAAGATGCCTCTCAACTCTTAGGGAATGACGATATTGTGGTGGCGATTGACGCGGGACATGGCGGTGAAGA
TCCTGGTTCCATTGGTCCTACACGCAAATATGAAAAAGACATCACCTTGAGCGTGTCTAAAAAGTTGGCGGATCAACTCA
ATGCAGTGCCGGGAATGAAAGCGGTACTGACTCGCCGTGGTGACTATTTTGTAAATCTGAATAAACGTACGGAAATTGCG
CGTCGTAGCAAGGCGCACTTGTTGGTTTCGGTGCATGCGGATGCGTTTCATACTCCACAGCCGCGCGGGGGATCGGTGTT
TGTGCTGAATACCCGCCGTGCCAATACCGAAATTGCCCGTTGGGTGGAAAATCACGAGCAACAATCTGAATTGCTGGGCG
GTGCTGGCGAAGTACTTTCGAAAACCAATAACGATCGTAACGTGAGTCAGACCCTACTGGATCTGCAGTTTAGCCATTCG
CAAAAAGAGGGCTATAAAGTCGCGACGAATATTTTGCGTGAAATGGGCAAGGTGGCGCATCTGCATAAAACCGAACCCGT
TAATGCAAGTTTGGCGGTATTGAAGTCACCAGACATCCCATCGGTACTGGTGGAAACAGGCTTTATCTCTAACCCATCAG
AAGAAAAATTGCTGATTCAGCGCAGTCATCAAGATAAGTTAGCTCGCGCTTTAGCCACGGCGATTGTGCAGTACTTTGAA
GATAATCCGCCAGAGGGCACACTGTTTGCCAACCGAGGTAAAGCTCAGAAACACAAAGTGCAGCGTGGAGAATCAATTGG
TCTGATTGCTAACCAGTATGGGGTCAGTGTCGATGCTTTGAAAAAAGCCAACAACCTCAAGTCTTCGACGATTTCGGTTG
GGCAACTGCTGACTATTCCAGCTTCTTCAGCACCAAACCCAGTACCTGTTCCTGTGATGGCAAATCCTGTAGAAACGGAA
ACTATTACCCATGTGGTAAAAACGGGCGATTTTCTCGGTAAACTTGCCACCACCTATAAGGTTTCAGTGGCTTCGATAAA
AAAAGAGAACAATCTGAAATCGGATACTCTAGTGCTCGGGCAGAAGCTGAAAATCACGGTGAGTTTAAAAGATAAGCCGC
TGCGTAAGCACAAGGTGCAGCGCGGTGAATTCCTGAGCAAAATCGCTGATCAATACAATGTTAGCGTTGATAGCATTCGC
CAAGCCAACCAGCTACGCACTGACCAATTGTTGGTTGGGCAGCAACTGATTATCCCGAATAAATAA

Upstream 100 bases:

>100_bases
AAAAAGGGCATGGACTATTGCCAAATGCGGATCTTGATATCGATTTGCGTTACGACGGCGATCAGCGAGTTGCTACGCTG
ACAGCCAATAACCATTATGG

Downstream 100 bases:

>100_bases
GCGCTCATGACGATTCGAATCCTACCCGCCCGTTTAGCCAACCAAATTGCAGCGGGTGAAGTTGTAGAAAGACCGGCTTC
AGTAGTCAAGGAGTTGGTGG

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 581; Mature: 581

Protein sequence:

>581_residues
MRPFESVGVMLNVSRFVFLLFGFVLLAIAPQTWANVLEGVRVWPSPDETRVVLDVKSEVDYSYFTLSSPERLVVDLKQST
SRAKLPVNVTESGILSKVRASSPPEKSTFRLVFELKQKTTPTLFKLAPTPGGQYGHRLVIDMPHGKVSESSSASTPSSPA
QVSKDASQLLGNDDIVVAIDAGHGGEDPGSIGPTRKYEKDITLSVSKKLADQLNAVPGMKAVLTRRGDYFVNLNKRTEIA
RRSKAHLLVSVHADAFHTPQPRGGSVFVLNTRRANTEIARWVENHEQQSELLGGAGEVLSKTNNDRNVSQTLLDLQFSHS
QKEGYKVATNILREMGKVAHLHKTEPVNASLAVLKSPDIPSVLVETGFISNPSEEKLLIQRSHQDKLARALATAIVQYFE
DNPPEGTLFANRGKAQKHKVQRGESIGLIANQYGVSVDALKKANNLKSSTISVGQLLTIPASSAPNPVPVPVMANPVETE
TITHVVKTGDFLGKLATTYKVSVASIKKENNLKSDTLVLGQKLKITVSLKDKPLRKHKVQRGEFLSKIADQYNVSVDSIR
QANQLRTDQLLVGQQLIIPNK

Sequences:

>Translated_581_residues
MRPFESVGVMLNVSRFVFLLFGFVLLAIAPQTWANVLEGVRVWPSPDETRVVLDVKSEVDYSYFTLSSPERLVVDLKQST
SRAKLPVNVTESGILSKVRASSPPEKSTFRLVFELKQKTTPTLFKLAPTPGGQYGHRLVIDMPHGKVSESSSASTPSSPA
QVSKDASQLLGNDDIVVAIDAGHGGEDPGSIGPTRKYEKDITLSVSKKLADQLNAVPGMKAVLTRRGDYFVNLNKRTEIA
RRSKAHLLVSVHADAFHTPQPRGGSVFVLNTRRANTEIARWVENHEQQSELLGGAGEVLSKTNNDRNVSQTLLDLQFSHS
QKEGYKVATNILREMGKVAHLHKTEPVNASLAVLKSPDIPSVLVETGFISNPSEEKLLIQRSHQDKLARALATAIVQYFE
DNPPEGTLFANRGKAQKHKVQRGESIGLIANQYGVSVDALKKANNLKSSTISVGQLLTIPASSAPNPVPVPVMANPVETE
TITHVVKTGDFLGKLATTYKVSVASIKKENNLKSDTLVLGQKLKITVSLKDKPLRKHKVQRGEFLSKIADQYNVSVDSIR
QANQLRTDQLLVGQQLIIPNK
>Mature_581_residues
MRPFESVGVMLNVSRFVFLLFGFVLLAIAPQTWANVLEGVRVWPSPDETRVVLDVKSEVDYSYFTLSSPERLVVDLKQST
SRAKLPVNVTESGILSKVRASSPPEKSTFRLVFELKQKTTPTLFKLAPTPGGQYGHRLVIDMPHGKVSESSSASTPSSPA
QVSKDASQLLGNDDIVVAIDAGHGGEDPGSIGPTRKYEKDITLSVSKKLADQLNAVPGMKAVLTRRGDYFVNLNKRTEIA
RRSKAHLLVSVHADAFHTPQPRGGSVFVLNTRRANTEIARWVENHEQQSELLGGAGEVLSKTNNDRNVSQTLLDLQFSHS
QKEGYKVATNILREMGKVAHLHKTEPVNASLAVLKSPDIPSVLVETGFISNPSEEKLLIQRSHQDKLARALATAIVQYFE
DNPPEGTLFANRGKAQKHKVQRGESIGLIANQYGVSVDALKKANNLKSSTISVGQLLTIPASSAPNPVPVPVMANPVETE
TITHVVKTGDFLGKLATTYKVSVASIKKENNLKSDTLVLGQKLKITVSLKDKPLRKHKVQRGEFLSKIADQYNVSVDSIR
QANQLRTDQLLVGQQLIIPNK

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1790611, Length=418, Percent_Identity=38.2775119617225, Blast_Score=276, Evalue=3e-75,
Organism=Escherichia coli, GI87082163, Length=413, Percent_Identity=34.6246973365617, Blast_Score=234, Evalue=1e-62,
Organism=Escherichia coli, GI1788776, Length=225, Percent_Identity=38.2222222222222, Blast_Score=155, Evalue=7e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508 [H]

Pfam domain/function: PF01520 Amidase_3 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 63549; Mature: 63549

Theoretical pI: Translated: 10.30; Mature: 10.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPFESVGVMLNVSRFVFLLFGFVLLAIAPQTWANVLEGVRVWPSPDETRVVLDVKSEVD
CCCHHHHHHEEEHHHHHHHHHHHHHHHHCCHHHHHHHCCCEECCCCCCCEEEEEECCCCC
YSYFTLSSPERLVVDLKQSTSRAKLPVNVTESGILSKVRASSPPEKSTFRLVFELKQKTT
EEEEEECCCCEEEEEEHHCCCCCCCCEECCCHHHHHHHHCCCCCCCCCEEEEEEHHHCCC
PTLFKLAPTPGGQYGHRLVIDMPHGKVSESSSASTPSSPAQVSKDASQLLGNDDIVVAID
CCEEEECCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEE
AGHGGEDPGSIGPTRKYEKDITLSVSKKLADQLNAVPGMKAVLTRRGDYFVNLNKRTEIA
CCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHCCCCHHHHHHCCCCEEEEECCHHHHH
RRSKAHLLVSVHADAFHTPQPRGGSVFVLNTRRANTEIARWVENHEQQSELLGGAGEVLS
HCCCCEEEEEEECCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCHHHHHH
KTNNDRNVSQTLLDLQFSHSQKEGYKVATNILREMGKVAHLHKTEPVNASLAVLKSPDIP
CCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCC
SVLVETGFISNPSEEKLLIQRSHQDKLARALATAIVQYFEDNPPEGTLFANRGKAQKHKV
HHHHHCCCCCCCCCCHHEEECCHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHH
QRGESIGLIANQYGVSVDALKKANNLKSSTISVGQLLTIPASSAPNPVPVPVMANPVETE
HCCCCEEEEEHHHCCCHHHHHHHCCCCCCCEECCEEEEECCCCCCCCCCCCEECCCCCHH
TITHVVKTGDFLGKLATTYKVSVASIKKENNLKSDTLVLGQKLKITVSLKDKPLRKHKVQ
HHHHHHHHHHHHHHHHHHHEEHHHHHHHCCCCCCCEEEECCEEEEEEEECCCCHHHHHHH
RGEFLSKIADQYNVSVDSIRQANQLRTDQLLVGQQLIIPNK
HHHHHHHHHHHHCCCHHHHHHHHHHCHHHHHHCCEEECCCC
>Mature Secondary Structure
MRPFESVGVMLNVSRFVFLLFGFVLLAIAPQTWANVLEGVRVWPSPDETRVVLDVKSEVD
CCCHHHHHHEEEHHHHHHHHHHHHHHHHCCHHHHHHHCCCEECCCCCCCEEEEEECCCCC
YSYFTLSSPERLVVDLKQSTSRAKLPVNVTESGILSKVRASSPPEKSTFRLVFELKQKTT
EEEEEECCCCEEEEEEHHCCCCCCCCEECCCHHHHHHHHCCCCCCCCCEEEEEEHHHCCC
PTLFKLAPTPGGQYGHRLVIDMPHGKVSESSSASTPSSPAQVSKDASQLLGNDDIVVAID
CCEEEECCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEE
AGHGGEDPGSIGPTRKYEKDITLSVSKKLADQLNAVPGMKAVLTRRGDYFVNLNKRTEIA
CCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHCCCCHHHHHHCCCCEEEEECCHHHHH
RRSKAHLLVSVHADAFHTPQPRGGSVFVLNTRRANTEIARWVENHEQQSELLGGAGEVLS
HCCCCEEEEEEECCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCHHHHHH
KTNNDRNVSQTLLDLQFSHSQKEGYKVATNILREMGKVAHLHKTEPVNASLAVLKSPDIP
CCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCC
SVLVETGFISNPSEEKLLIQRSHQDKLARALATAIVQYFEDNPPEGTLFANRGKAQKHKV
HHHHHCCCCCCCCCCHHEEECCHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHH
QRGESIGLIANQYGVSVDALKKANNLKSSTISVGQLLTIPASSAPNPVPVPVMANPVETE
HCCCCEEEEEHHHCCCHHHHHHHCCCCCCCEECCEEEEECCCCCCCCCCCCEECCCCCHH
TITHVVKTGDFLGKLATTYKVSVASIKKENNLKSDTLVLGQKLKITVSLKDKPLRKHKVQ
HHHHHHHHHHHHHHHHHHHEEHHHHHHHCCCCCCCEEEECCEEEEEEEECCCCHHHHHHH
RGEFLSKIADQYNVSVDSIRQANQLRTDQLLVGQQLIIPNK
HHHHHHHHHHHHCCCHHHHHHHHHHCHHHHHHCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609; 2676972 [H]