Click here to switch to the map view.

The map label for this gene is nusG

Identifier: 227080535

GI number: 227080535

Start: 321856

End: 322404

Strand: Direct

Name: nusG

Synonym: VCM66_0307

Alternate gene names: 227080535

Gene position: 321856-322404 (Clockwise)

Preceding gene: 227080534

Following gene: 227080536

Centisome position: 11.13

GC content: 46.99

Gene sequence:

>549_bases
ATGAGTGAAGCTCCCAAAAAACGCTGGTATGTAGTTCAAGCCTTCTCGGGATTTGAAGGACGTGTTGCTCAGTCACTTCG
TGAACATATCAAAATGCACGGTATGGAAGAGCTGTTTGGTGAAGTGTTAGTACCAACAGAAGAAGTGGTAGAAATGCGCG
CTGGCCAACGCCGCAAAAGTGAACGTAAATTCTTCCCAGGTTATGTGCTAGTGCAGATGATCATGAATGATGAATCTTGG
CACTTGGTACGTAGCGTACCTCGCGTGATGGGATTCATCGGTGGTACATCAGATCGCCCAGCTCCTATCACAGATAAGGA
AGCGGATGCGATTCTGAATCGTCTTGAGAAAGCCAGCGAAGCACCACGTCCTAAAACCATGTTTGAAGCAGGTGAAGTCG
TGCGTGTTAACGACGGTCCATTTGCTGACTTCAACGGTACCGTTGAAGAGGTGGATTACGAGAAAAGCCGCTTGAAAGTG
TCGGTTTCGATCTTTGGTCGTGCAACACCTGTTGAACTCGAATTTGGTCAAGTGGAAAAGCTTGATTAA

Upstream 100 bases:

>100_bases
AGCAGTAAGTATTGTAATGGCTCTGGCGCTTTGGGGTATTGACGGCATTATGGTTCGTCTAGTGGCCTTTGCGACCGGAG
TATAGTAGAGGGTTTAATAC

Downstream 100 bases:

>100_bases
AAAAGCATCTCTTTAGGCTTGTCTGGGGCGTGGATTATGACTATAATTTCGCGCCTTTTTACAGGTTCTTTATGAAAACC
GGGGAGCTGATCAATCGATT

Product: transcription antitermination protein NusG

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 182; Mature: 181

Protein sequence:

>182_residues
MSEAPKKRWYVVQAFSGFEGRVAQSLREHIKMHGMEELFGEVLVPTEEVVEMRAGQRRKSERKFFPGYVLVQMIMNDESW
HLVRSVPRVMGFIGGTSDRPAPITDKEADAILNRLEKASEAPRPKTMFEAGEVVRVNDGPFADFNGTVEEVDYEKSRLKV
SVSIFGRATPVELEFGQVEKLD

Sequences:

>Translated_182_residues
MSEAPKKRWYVVQAFSGFEGRVAQSLREHIKMHGMEELFGEVLVPTEEVVEMRAGQRRKSERKFFPGYVLVQMIMNDESW
HLVRSVPRVMGFIGGTSDRPAPITDKEADAILNRLEKASEAPRPKTMFEAGEVVRVNDGPFADFNGTVEEVDYEKSRLKV
SVSIFGRATPVELEFGQVEKLD
>Mature_181_residues
SEAPKKRWYVVQAFSGFEGRVAQSLREHIKMHGMEELFGEVLVPTEEVVEMRAGQRRKSERKFFPGYVLVQMIMNDESWH
LVRSVPRVMGFIGGTSDRPAPITDKEADAILNRLEKASEAPRPKTMFEAGEVVRVNDGPFADFNGTVEEVDYEKSRLKVS
VSIFGRATPVELEFGQVEKLD

Specific function: Influences transcription termination and antitermination. Acts as a component of the transcription complex, and interacts with the termination factor rho and RNA polymerase

COG id: COG0250

COG function: function code K; Transcription antiterminator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 KOW domain

Homologues:

Organism=Escherichia coli, GI1790414, Length=180, Percent_Identity=86.6666666666667, Blast_Score=328, Evalue=1e-91,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NUSG_VIBCH (Q9KV35)

Other databases:

- EMBL:   AE003852
- PIR:   A82338
- RefSeq:   NP_229977.1
- ProteinModelPortal:   Q9KV35
- SMR:   Q9KV35
- GeneID:   2615089
- GenomeReviews:   AE003852_GR
- KEGG:   vch:VC0323
- TIGR:   VC_0323
- HOGENOM:   HBG751162
- OMA:   VASGCEK
- ProtClustDB:   PRK05609
- InterPro:   IPR005824
- InterPro:   IPR001062
- InterPro:   IPR015869
- InterPro:   IPR006645
- InterPro:   IPR014722
- InterPro:   IPR008991
- Gene3D:   G3DSA:2.30.30.30
- Gene3D:   G3DSA:3.30.70.940
- PRINTS:   PR00338
- SMART:   SM00739
- SMART:   SM00738
- TIGRFAMs:   TIGR00922

Pfam domain/function: PF00467 KOW; PF02357 NusG; SSF82679 Transcrpt_antiterm_NusG_N; SSF50104 Transl_SH3_like

EC number: NA

Molecular weight: Translated: 20651; Mature: 20520

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS01014 NUSG

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEAPKKRWYVVQAFSGFEGRVAQSLREHIKMHGMEELFGEVLVPTEEVVEMRAGQRRKS
CCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHCHHHHH
ERKFFPGYVLVQMIMNDESWHLVRSVPRVMGFIGGTSDRPAPITDKEADAILNRLEKASE
HCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHC
APRPKTMFEAGEVVRVNDGPFADFNGTVEEVDYEKSRLKVSVSIFGRATPVELEFGQVEK
CCCCCHHHCCCCEEEECCCCCCCCCCCHHHHCCCCHHEEEEEEEECCCCCEEECCCCCCC
LD
CC
>Mature Secondary Structure 
SEAPKKRWYVVQAFSGFEGRVAQSLREHIKMHGMEELFGEVLVPTEEVVEMRAGQRRKS
CCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHCHHHHH
ERKFFPGYVLVQMIMNDESWHLVRSVPRVMGFIGGTSDRPAPITDKEADAILNRLEKASE
HCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHC
APRPKTMFEAGEVVRVNDGPFADFNGTVEEVDYEKSRLKVSVSIFGRATPVELEFGQVEK
CCCCCHHHCCCCEEEECCCCCCCCCCCHHHHCCCCHHEEEEEEEECCCCCEEECCCCCCC
LD
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301