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The map label for this gene is lexA

Identifier: 227080328

GI number: 227080328

Start: 89978

End: 90607

Strand: Reverse

Name: lexA

Synonym: VCM66_0092

Alternate gene names: 227080328

Gene position: 90607-89978 (Counterclockwise)

Preceding gene: 227080330

Following gene: 227080327

Centisome position: 3.13

GC content: 52.7

Gene sequence:

>630_bases
ATGAAGCCGTTAACCCCTCGCCAACAAGAAGTGTTTGATCTGATCAAAAGTAAGATCGATGAAACCGGAATGCCCCCAAC
CCGCGCCGAAATTGCTAAAGAGCTGGGTTTTCGTTCGGCCAATGCGGCTGAAGAACACCTCAAAGCGCTGGCTCGTAAAC
AAGTGATTGAAATGGTGCCCGGCGCCTCACGCGGGATCCGCATCCTCGTCGATAACGCCGCAAACGAAGAAGAAGCCGAA
ACTGGCTTGCCTTTGATTGGCCGCGTTGCCGCGGGTGAACCGATTCTTGCTCAAGAGCATGTGGAAGCACACTACCAAGT
CGATCCGAGCATGTTCCGCCCGCAAGCCGATTTTCTGCTGCGTGTACATGGCGAAAGTATGAAGAACATCGGCATCCTCG
ATGGTGACTTACTTGCTGTGCACAAAACACAAGATGTACGCAACGGCCAAGTCGTGGTGGCGCGCGTAGAAGATGACGTC
ACGGTGAAACGTCTGGAGCGCAAAGGTTCCAAAGTTTTCCTGCATGCGGAAAATGAAGAGTTTGCGCCAATCGAAGTCGA
TTTAGCCGCGCAATCGCTCACCATTGAAGGCATTGCAGTAGGCGTCATTCGCAACAGCACTTGGATGTGA

Upstream 100 bases:

>100_bases
TGGCGAACTTCACAATCACACAAAAAACAAGCAATTGGCTTTGCAATTCTCATTGCACTGGATATACTCACAGTCAACTG
TATAAAAAGACAGGTGACTC

Downstream 100 bases:

>100_bases
AGATTGTTGAGATAAATTCTCAACAACTTGCCTTCTTATTTGAGATTCATTATCATCTTCCTATCCCAGTATAGGAAGAT
GAGCATGCGCCACTCAGATT

Product: LexA repressor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 209; Mature: 209

Protein sequence:

>209_residues
MKPLTPRQQEVFDLIKSKIDETGMPPTRAEIAKELGFRSANAAEEHLKALARKQVIEMVPGASRGIRILVDNAANEEEAE
TGLPLIGRVAAGEPILAQEHVEAHYQVDPSMFRPQADFLLRVHGESMKNIGILDGDLLAVHKTQDVRNGQVVVARVEDDV
TVKRLERKGSKVFLHAENEEFAPIEVDLAAQSLTIEGIAVGVIRNSTWM

Sequences:

>Translated_209_residues
MKPLTPRQQEVFDLIKSKIDETGMPPTRAEIAKELGFRSANAAEEHLKALARKQVIEMVPGASRGIRILVDNAANEEEAE
TGLPLIGRVAAGEPILAQEHVEAHYQVDPSMFRPQADFLLRVHGESMKNIGILDGDLLAVHKTQDVRNGQVVVARVEDDV
TVKRLERKGSKVFLHAENEEFAPIEVDLAAQSLTIEGIAVGVIRNSTWM
>Mature_209_residues
MKPLTPRQQEVFDLIKSKIDETGMPPTRAEIAKELGFRSANAAEEHLKALARKQVIEMVPGASRGIRILVDNAANEEEAE
TGLPLIGRVAAGEPILAQEHVEAHYQVDPSMFRPQADFLLRVHGESMKNIGILDGDLLAVHKTQDVRNGQVVVARVEDDV
TVKRLERKGSKVFLHAENEEFAPIEVDLAAQSLTIEGIAVGVIRNSTWM

Specific function: Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, recA interacts with lexA causing an autocatalytic cleavage which disrupts the DNA-binding part of lexA, lea

COG id: COG1974

COG function: function code KT; SOS-response transcriptional repressors (RecA-mediated autopeptidases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S24 family

Homologues:

Organism=Escherichia coli, GI1790476, Length=209, Percent_Identity=73.2057416267943, Blast_Score=309, Evalue=7e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEXA_VIBC3 (A5F4F6)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F4F6
- SMR:   A5F4F6
- STRING:   A5F4F6
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A2423
- eggNOG:   COG1974
- HOGENOM:   HBG679610
- OMA:   VGVWRGY
- ProtClustDB:   PRK00215
- BioCyc:   VCHO345073:VC0395_A2423-MONOMER
- GO:   GO:0006508
- GO:   GO:0006350
- HAMAP:   MF_00015
- InterPro:   IPR006199
- InterPro:   IPR006200
- InterPro:   IPR006197
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056
- InterPro:   IPR011991
- Gene3D:   G3DSA:2.10.109.10
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00726
- TIGRFAMs:   TIGR00498

Pfam domain/function: PF01726 LexA_DNA_bind; PF00717 Peptidase_S24; SSF51306 Pept_S24_S26_C

EC number: =3.4.21.88

Molecular weight: Translated: 23030; Mature: 23030

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: NA

Important sites: ACT_SITE 126-126 ACT_SITE 163-163

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPLTPRQQEVFDLIKSKIDETGMPPTRAEIAKELGFRSANAAEEHLKALARKQVIEMVP
CCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCC
GASRGIRILVDNAANEEEAETGLPLIGRVAAGEPILAQEHVEAHYQVDPSMFRPQADFLL
CCCCCEEEEEECCCCCHHHHCCCCEEEECCCCCCCCHHHHHHHHEEECHHHHCCCCCEEE
RVHGESMKNIGILDGDLLAVHKTQDVRNGQVVVARVEDDVTVKRLERKGSKVFLHAENEE
EECCCCCCCCCCCCCCEEEEECCCCCCCCCEEEEEECCCHHHHHHHCCCCEEEEEECCCC
FAPIEVDLAAQSLTIEGIAVGVIRNSTWM
CCCEEEEECCCEEEECEEEEEEEECCCCC
>Mature Secondary Structure
MKPLTPRQQEVFDLIKSKIDETGMPPTRAEIAKELGFRSANAAEEHLKALARKQVIEMVP
CCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCC
GASRGIRILVDNAANEEEAETGLPLIGRVAAGEPILAQEHVEAHYQVDPSMFRPQADFLL
CCCCCEEEEEECCCCCHHHHCCCCEEEECCCCCCCCHHHHHHHHEEECHHHHCCCCCEEE
RVHGESMKNIGILDGDLLAVHKTQDVRNGQVVVARVEDDVTVKRLERKGSKVFLHAENEE
EECCCCCCCCCCCCCCEEEEECCCCCCCCCEEEEEECCCHHHHHHHCCCCEEEEEECCCC
FAPIEVDLAAQSLTIEGIAVGVIRNSTWM
CCCEEEEECCCEEEECEEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA