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The map label for this gene is trmE [H]

Identifier: 227080240

GI number: 227080240

Start: 816

End: 2210

Strand: Reverse

Name: trmE [H]

Synonym: VCM66_0003

Alternate gene names: 227080240

Gene position: 2210-816 (Counterclockwise)

Preceding gene: 227080241

Following gene: 227080239

Centisome position: 0.08

GC content: 52.47

Gene sequence:

>1395_bases
ATGGCGCTAATTCAATCCTGTTCAGGTAACACTATGACAACAGATACCATTGTCGCGCAAGCTACGGCACCCGGCCGTGG
TGGTGTCGGCATTATTCGTGTATCAGGCCCACTGGCTGCCCACGTCGCGCAGACAGTCACTGGACGCACGCTCAGACCCC
GCTATGCCGAATACTTGCCTTTCACCGATGAAGATGGCCAACAGCTCGATCAAGGCATTGCGCTGTTTTTCCCCAATCCT
CATTCATTTACCGGTGAAGATGTGCTTGAGCTGCAAGGTCACGGTGGCCCGGTCGTCATGGATATGCTGATCCGCCGTAT
TTTGCAGATTAAAGGAGTGCGCCCAGCCCGTCCCGGTGAATTCTCAGAACGCGCTTTCCTCAATGACAAGATGGATTTAA
CCCAAGCGGAAGCCATCGCAGACCTGATTGATGCCAGCTCTGAGCAAGCCGCGAAATCCGCGCTGCAATCACTGCAAGGC
GAGTTTTCTAAACGCATCCATACCTTGGTGGAATCGCTGATTCACCTGCGCATCTATGTTGAAGCCGCAATTGATTTCCC
AGAAGAAGAGATCGATTTTCTGGCCGATGGTAAGGTTTCCGCTGATCTGCAAACCATCATAGATAATCTTGCCGCAGTGC
GCCGTGAAGCCAACCAAGGCGCCATTATGCGCGAAGGGATGAAGGTGGTGATTGCTGGGCGTCCTAATGCGGGTAAATCG
AGCCTACTCAATGCGCTTTCAGGTAAAGAGTCTGCCATCGTTACCGACATTGCCGGTACCACCCGTGATGTTCTGCGTGA
GCATATCCATATTGATGGGATGCCGCTGCACATCATCGATACTGCGGGACTACGAGACGCTTCTGATGCGGTAGAAAAAA
TTGGTATTGAGCGCGCATGGGAAGAGATCCGCCAAGCCGATCGCGTACTGTTTATGGTCGATGGCACCACCACAGAGGCC
ACCGATCCACAGGACATCTGGCCTGATTTTGTTGACAAACTGCCAGAGAATATTGGGATCACTGTGATCCGTAATAAAGC
CGATCAAACTGGTGAGCCCCTTGGGATCTGCCATGTTAATCAACCCACCTTGATCCGCCTATCAGCCAAAACAGGCCAAG
GGGTCGATGCCTTGCGTCAGCACCTCAAAGAGTGCATGGGTTTTTCAGGCAATCAGGAAGGTGGCTTTATGGCACGCCGC
CGTCATCTCGATGCCCTAGAGCGAGCCGCAGAGCACTTAGCGATTGGCCAGCAACAACTTGAAGGCTATATGGCCGGAGA
AATCCTCGCCGAAGAGCTGCGTATAGCCCAGCAACACCTTAACGAAATTACGGGCGAGTTCAGCTCCGATGATCTGCTTG
GCCGCATTTTTTCATCATTCTGTATTGGGAAATAG

Upstream 100 bases:

>100_bases
CTACAAAGCGCTAGAGAAAAAAGGCTTACACTCCAAGTAAGTTTGATTGAGTAATAAAGAGGCGGCCAAAAGGTCGCCTT
TTTGTTGGCTGCTGATTACA

Downstream 100 bases:

>100_bases
AGTAACGCTATGATTCATATCATTACTGGCAGCACCCTAGGCGGTGCGGAATATGTGGGTGACCACCTGAGTGATTTACT
GCAAGAACAAGGCTTTGACA

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 464; Mature: 463

Protein sequence:

>464_residues
MALIQSCSGNTMTTDTIVAQATAPGRGGVGIIRVSGPLAAHVAQTVTGRTLRPRYAEYLPFTDEDGQQLDQGIALFFPNP
HSFTGEDVLELQGHGGPVVMDMLIRRILQIKGVRPARPGEFSERAFLNDKMDLTQAEAIADLIDASSEQAAKSALQSLQG
EFSKRIHTLVESLIHLRIYVEAAIDFPEEEIDFLADGKVSADLQTIIDNLAAVRREANQGAIMREGMKVVIAGRPNAGKS
SLLNALSGKESAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRDASDAVEKIGIERAWEEIRQADRVLFMVDGTTTEA
TDPQDIWPDFVDKLPENIGITVIRNKADQTGEPLGICHVNQPTLIRLSAKTGQGVDALRQHLKECMGFSGNQEGGFMARR
RHLDALERAAEHLAIGQQQLEGYMAGEILAEELRIAQQHLNEITGEFSSDDLLGRIFSSFCIGK

Sequences:

>Translated_464_residues
MALIQSCSGNTMTTDTIVAQATAPGRGGVGIIRVSGPLAAHVAQTVTGRTLRPRYAEYLPFTDEDGQQLDQGIALFFPNP
HSFTGEDVLELQGHGGPVVMDMLIRRILQIKGVRPARPGEFSERAFLNDKMDLTQAEAIADLIDASSEQAAKSALQSLQG
EFSKRIHTLVESLIHLRIYVEAAIDFPEEEIDFLADGKVSADLQTIIDNLAAVRREANQGAIMREGMKVVIAGRPNAGKS
SLLNALSGKESAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRDASDAVEKIGIERAWEEIRQADRVLFMVDGTTTEA
TDPQDIWPDFVDKLPENIGITVIRNKADQTGEPLGICHVNQPTLIRLSAKTGQGVDALRQHLKECMGFSGNQEGGFMARR
RHLDALERAAEHLAIGQQQLEGYMAGEILAEELRIAQQHLNEITGEFSSDDLLGRIFSSFCIGK
>Mature_463_residues
ALIQSCSGNTMTTDTIVAQATAPGRGGVGIIRVSGPLAAHVAQTVTGRTLRPRYAEYLPFTDEDGQQLDQGIALFFPNPH
SFTGEDVLELQGHGGPVVMDMLIRRILQIKGVRPARPGEFSERAFLNDKMDLTQAEAIADLIDASSEQAAKSALQSLQGE
FSKRIHTLVESLIHLRIYVEAAIDFPEEEIDFLADGKVSADLQTIIDNLAAVRREANQGAIMREGMKVVIAGRPNAGKSS
LLNALSGKESAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRDASDAVEKIGIERAWEEIRQADRVLFMVDGTTTEAT
DPQDIWPDFVDKLPENIGITVIRNKADQTGEPLGICHVNQPTLIRLSAKTGQGVDALRQHLKECMGFSGNQEGGFMARRR
HLDALERAAEHLAIGQQQLEGYMAGEILAEELRIAQQHLNEITGEFSSDDLLGRIFSSFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI193082991, Length=468, Percent_Identity=35.4700854700855, Blast_Score=250, Evalue=2e-66,
Organism=Homo sapiens, GI193082988, Length=500, Percent_Identity=33.6, Blast_Score=238, Evalue=1e-62,
Organism=Homo sapiens, GI193082993, Length=458, Percent_Identity=35.3711790393013, Blast_Score=235, Evalue=8e-62,
Organism=Escherichia coli, GI2367268, Length=451, Percent_Identity=75.3880266075388, Blast_Score=698, Evalue=0.0,
Organism=Escherichia coli, GI87082120, Length=206, Percent_Identity=30.5825242718447, Blast_Score=71, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17507259, Length=454, Percent_Identity=28.6343612334802, Blast_Score=170, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6323665, Length=497, Percent_Identity=31.1871227364185, Blast_Score=211, Evalue=2e-55,
Organism=Drosophila melanogaster, GI45550813, Length=488, Percent_Identity=33.4016393442623, Blast_Score=212, Evalue=4e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 50569; Mature: 50438

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALIQSCSGNTMTTDTIVAQATAPGRGGVGIIRVSGPLAAHVAQTVTGRTLRPRYAEYLP
CCCCCCCCCCCEECHHEEEEECCCCCCCEEEEEECCHHHHHHHHHHCCCCCCCCHHHHCC
FTDEDGQQLDQGIALFFPNPHSFTGEDVLELQGHGGPVVMDMLIRRILQIKGVRPARPGE
CCCCCHHHHHCCEEEEECCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCC
FSERAFLNDKMDLTQAEAIADLIDASSEQAAKSALQSLQGEFSKRIHTLVESLIHLRIYV
CCHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
EAAIDFPEEEIDFLADGKVSADLQTIIDNLAAVRREANQGAIMREGMKVVIAGRPNAGKS
EHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHCCCEEEEECCCCCCHH
SLLNALSGKESAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRDASDAVEKIGIERAW
HHHHHHCCCCCEEEECCCCCHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHH
EEIRQADRVLFMVDGTTTEATDPQDIWPDFVDKLPENIGITVIRNKADQTGEPLGICHVN
HHHHHHCCEEEEEECCCCCCCCCHHCCHHHHHHCCCCCCEEEEECCCCCCCCCEEEEECC
QPTLIRLSAKTGQGVDALRQHLKECMGFSGNQEGGFMARRRHLDALERAAEHLAIGQQQL
CCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EGYMAGEILAEELRIAQQHLNEITGEFSSDDLLGRIFSSFCIGK
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
ALIQSCSGNTMTTDTIVAQATAPGRGGVGIIRVSGPLAAHVAQTVTGRTLRPRYAEYLP
CCCCCCCCCCEECHHEEEEECCCCCCCEEEEEECCHHHHHHHHHHCCCCCCCCHHHHCC
FTDEDGQQLDQGIALFFPNPHSFTGEDVLELQGHGGPVVMDMLIRRILQIKGVRPARPGE
CCCCCHHHHHCCEEEEECCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCC
FSERAFLNDKMDLTQAEAIADLIDASSEQAAKSALQSLQGEFSKRIHTLVESLIHLRIYV
CCHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
EAAIDFPEEEIDFLADGKVSADLQTIIDNLAAVRREANQGAIMREGMKVVIAGRPNAGKS
EHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHCCCEEEEECCCCCCHH
SLLNALSGKESAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRDASDAVEKIGIERAW
HHHHHHCCCCCEEEECCCCCHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHH
EEIRQADRVLFMVDGTTTEATDPQDIWPDFVDKLPENIGITVIRNKADQTGEPLGICHVN
HHHHHHCCEEEEEECCCCCCCCCHHCCHHHHHHCCCCCCEEEEECCCCCCCCCEEEEECC
QPTLIRLSAKTGQGVDALRQHLKECMGFSGNQEGGFMARRRHLDALERAAEHLAIGQQQL
CCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EGYMAGEILAEELRIAQQHLNEITGEFSSDDLLGRIFSSFCIGK
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA