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The map label for this gene is truA [H]

Identifier: 226950899

GI number: 226950899

Start: 3956605

End: 3957345

Strand: Reverse

Name: truA [H]

Synonym: CLM_3914

Alternate gene names: 226950899

Gene position: 3957345-3956605 (Counterclockwise)

Preceding gene: 226950900

Following gene: 226950898

Centisome position: 95.24

GC content: 27.94

Gene sequence:

>741_bases
GTGAAAAATATAAAATTGAAACTTGAGTATGATGGAGCAAATTATTGTGGATGGCAAAAACAAAAAAATGAAAAGTTTAT
TACGTTACAAGGTACTTTAGAAAAAGCTATAAGTAATATAACTAAAGAAAAAATAGAAGTTATAGGAGCCAGTAGAACAG
ATTCGGGGGTCCATGCTAAAGGATATATATGTAATTTTTTTACAAACACTAAAATACCACCTGAAAACTTACAAAAAGTA
ATTAATAATAATTTACCACCAGATATAGTAGTTTTAAGTTCAGAGGAAGCTAGTAGTGAGTTCCATTCTAGATTTTGTAG
TAAGGGCAAGACTTATGAATATACAGTTCTAAATAGCTCTAAGCCTATAGCCATAGGCAGAAATTATATTTTTCAGTTTA
AAGATAAACTCAATATAGATAATATGAAGATGGCATCAAGATATTTTATAGGAACACATGATTTCTCTGCTTTTAAAACA
AAGGGAAGTAGTATAAAAACTTCAGTAAGAACTATAAGTAAATTTGAAATAAATAAAAAGGGTGATTTTATAAAGTTTAT
AATTACAGGAGATGGTTTTCTTTATAACATGGTAAGAATAATAGTAGGTACATTGATAGAAATTGGCCTAAATAAAAGAG
AACCAGAATATATAAAGTATGTTATAAAATCAAAGGATAGAAGAAAAGCAGGGAAATGTGTGCCTTCATCTGGACTGTGT
TTAAAGGAAGTATTTTATTAA

Upstream 100 bases:

>100_bases
CTAGAATGAAAGAACTTTCCCTTTCGTCAAGAGATTTTGTAGCAGCAGCATTTACTTCTATTTTAGTTATACTATCTATT
TGGAGTAGGATGTGGACTTA

Downstream 100 bases:

>100_bases
TACTATTGACACACCGGGCAGATTGTATTATAATAACTTTGTTTGTATAGTATAATAGATTCACTAGCCCCGGATCTTTA
TATAGAAGAACGTTTTATGT

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MKNIKLKLEYDGANYCGWQKQKNEKFITLQGTLEKAISNITKEKIEVIGASRTDSGVHAKGYICNFFTNTKIPPENLQKV
INNNLPPDIVVLSSEEASSEFHSRFCSKGKTYEYTVLNSSKPIAIGRNYIFQFKDKLNIDNMKMASRYFIGTHDFSAFKT
KGSSIKTSVRTISKFEINKKGDFIKFIITGDGFLYNMVRIIVGTLIEIGLNKREPEYIKYVIKSKDRRKAGKCVPSSGLC
LKEVFY

Sequences:

>Translated_246_residues
MKNIKLKLEYDGANYCGWQKQKNEKFITLQGTLEKAISNITKEKIEVIGASRTDSGVHAKGYICNFFTNTKIPPENLQKV
INNNLPPDIVVLSSEEASSEFHSRFCSKGKTYEYTVLNSSKPIAIGRNYIFQFKDKLNIDNMKMASRYFIGTHDFSAFKT
KGSSIKTSVRTISKFEINKKGDFIKFIITGDGFLYNMVRIIVGTLIEIGLNKREPEYIKYVIKSKDRRKAGKCVPSSGLC
LKEVFY
>Mature_246_residues
MKNIKLKLEYDGANYCGWQKQKNEKFITLQGTLEKAISNITKEKIEVIGASRTDSGVHAKGYICNFFTNTKIPPENLQKV
INNNLPPDIVVLSSEEASSEFHSRFCSKGKTYEYTVLNSSKPIAIGRNYIFQFKDKLNIDNMKMASRYFIGTHDFSAFKT
KGSSIKTSVRTISKFEINKKGDFIKFIITGDGFLYNMVRIIVGTLIEIGLNKREPEYIKYVIKSKDRRKAGKCVPSSGLC
LKEVFY

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=267, Percent_Identity=27.7153558052434, Blast_Score=112, Evalue=3e-25,
Organism=Escherichia coli, GI1788657, Length=243, Percent_Identity=31.6872427983539, Blast_Score=150, Evalue=9e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 27995; Mature: 27995

Theoretical pI: Translated: 10.12; Mature: 10.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNIKLKLEYDGANYCGWQKQKNEKFITLQGTLEKAISNITKEKIEVIGASRTDSGVHAK
CCCEEEEEEECCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHEECCCCCCCCEEC
GYICNFFTNTKIPPENLQKVINNNLPPDIVVLSSEEASSEFHSRFCSKGKTYEYTVLNSS
CEEEEEECCCCCCHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHCCCCEEEEEECCC
KPIAIGRNYIFQFKDKLNIDNMKMASRYFIGTHDFSAFKTKGSSIKTSVRTISKFEINKK
CCEEECCCEEEEECCCCCCCCEEHHHEEEEECCCCHHHHCCCCCHHHHHHHHHHEEECCC
GDFIKFIITGDGFLYNMVRIIVGTLIEIGLNKREPEYIKYVIKSKDRRKAGKCVPSSGLC
CCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHCCCCCCCCCH
LKEVFY
HHHHCC
>Mature Secondary Structure
MKNIKLKLEYDGANYCGWQKQKNEKFITLQGTLEKAISNITKEKIEVIGASRTDSGVHAK
CCCEEEEEEECCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHEECCCCCCCCEEC
GYICNFFTNTKIPPENLQKVINNNLPPDIVVLSSEEASSEFHSRFCSKGKTYEYTVLNSS
CEEEEEECCCCCCHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHCCCCEEEEEECCC
KPIAIGRNYIFQFKDKLNIDNMKMASRYFIGTHDFSAFKTKGSSIKTSVRTISKFEINKK
CCEEECCCEEEEECCCCCCCCEEHHHEEEEECCCCHHHHCCCCCHHHHHHHHHHEEECCC
GDFIKFIITGDGFLYNMVRIIVGTLIEIGLNKREPEYIKYVIKSKDRRKAGKCVPSSGLC
CCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHCCCCCCCCCH
LKEVFY
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]