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The map label for this gene is pbpA [H]

Identifier: 226950833

GI number: 226950833

Start: 3887710

End: 3889140

Strand: Reverse

Name: pbpA [H]

Synonym: CLM_3845

Alternate gene names: 226950833

Gene position: 3889140-3887710 (Counterclockwise)

Preceding gene: 226950834

Following gene: 226950832

Centisome position: 93.6

GC content: 32.77

Gene sequence:

>1431_bases
ATGAAGGATATTTCAAATAATATAAAAAAAGTTTTATTAGTTTTTTTAATATGCTTTATGGGACTTATAACCTATATAAC
CTATTTTGAAATAGTAGTTGGGCCTAAAATAGTAGATAGTACTTATAATAGAAGGTTATGGGTAAAAAGAAATGAAGTGT
TAAGAGGAACTATATATGATAGAAACATGAAAGCACTAACTAAGAGTGAAAGAGTAAACAGTGAATTGCAAAAAAGAGAA
TATACTGGTAATTCTATGTTTGCTCATGTGTTAGGATATATTAATGTAAAGTATGGACTAACAGGGCTAGAAAAAAAGTA
TGATAAAGAATTAATGTCAACAGATATAAAAGATGATTTAACAACTTTCTTTAAAAATAAAGGTAAGTTGGAACAAAAGG
TAGGACATAATTTAAAAACAACATTAGACTATACAGTTCAAAAGGCTGCTTATGATGCTTTAGGGGACAATAAGGGATCA
GTAGTTATATTAAATCCAAAAACAGGAGAAGTTTTAGGAATGGTATCTAAGCCTTCCTATGATCCTAATAAATTAGATGA
AATATGGGGAAGTATAAATAAAGATAAGAATATTCCCCTAATAAATAGAGCTACAGCAGGATTGTATCCTCCAGGATCTA
CTTTTAAAATAGTAACTGCTTTAAGTGCCTTAGAAAATATGTCTGGGGTAATGAATAGAACATTTCAAGACAATGGTAGT
TTAGATTTAGGGGGGGGATACTCCTTAAGCAACTATGGAGGAGCATCTTATGGAAGCCTTGATTTAAAAGGTGCCTTTGT
TCATTCAAGTAATGTAGTGTTTGGTAGTTTAGCCTTAGAAGTAGGTAATTCAAGATTAAAGGGTACTACAGAAAAATTTT
ATTTTAATAAAGAAGTTCCTACAGATGGTATAGCTATAGAAAGTAGTAAGTTCCCAACTTTAAAGAGCTATGAAAAAGGA
AGTATAGCTCAAAGTGGAATAGGTCAAAGTTCTGTATTAGTAACTCCTATGGAGATGGCTTTGGTTGGAGCTACAGTTGC
TAATGACGGGGTTATGATGAAACCTTATTTAGTAAAAGAAGTTTTAAACAGCAAAGGAGAATTAATAAGAACAATCCCTC
CAGAATCTAATGGAGAAATAGTAAGTAAAAATAATGCCAGAATAGTTAAAGATTTTATGAAAGGTGTTGTAGAGGAAGGT
AGTGGAAGAAATGCTAGTATAGAAGGTATTCAAGTGGCCGGAAAGACTGGAACAGCAGATTATAGCGAATCCATTAGTGG
AAGAGCGGCCGCACATTCTTGGTTTGTAGGTTTTGCACCTTATGATAATCCTCAAGTAGCTATAGCAGTAATTGTAGAAA
ACGGAGGTCAAGGTGGTATAGCTGCAGCTAGTATAGCTTCACAAGTAATGAGTACAGCGTTGAGCAAATAA

Upstream 100 bases:

>100_bases
ATAACATTACCTCTAGTAAGCCGTGGAGGAAGTTCCATGATGAGTATTTATATTTGTTTAGGTATATTACAAAAGATATC
AGAAGAGGGTAGATAAAATT

Downstream 100 bases:

>100_bases
TAAAATAGGGGTGAAGGATAAAGTCATATGAAAATTGCAGATATAACTAGTGAAAACAGTGGTGAAGTAGTAGAATTTAA
TGCTTTAGTTAATGATAAAA

Product: penicillin-binding protein

Products: NA

Alternate protein names: PBPA [H]

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MKDISNNIKKVLLVFLICFMGLITYITYFEIVVGPKIVDSTYNRRLWVKRNEVLRGTIYDRNMKALTKSERVNSELQKRE
YTGNSMFAHVLGYINVKYGLTGLEKKYDKELMSTDIKDDLTTFFKNKGKLEQKVGHNLKTTLDYTVQKAAYDALGDNKGS
VVILNPKTGEVLGMVSKPSYDPNKLDEIWGSINKDKNIPLINRATAGLYPPGSTFKIVTALSALENMSGVMNRTFQDNGS
LDLGGGYSLSNYGGASYGSLDLKGAFVHSSNVVFGSLALEVGNSRLKGTTEKFYFNKEVPTDGIAIESSKFPTLKSYEKG
SIAQSGIGQSSVLVTPMEMALVGATVANDGVMMKPYLVKEVLNSKGELIRTIPPESNGEIVSKNNARIVKDFMKGVVEEG
SGRNASIEGIQVAGKTGTADYSESISGRAAAHSWFVGFAPYDNPQVAIAVIVENGGQGGIAAASIASQVMSTALSK

Sequences:

>Translated_476_residues
MKDISNNIKKVLLVFLICFMGLITYITYFEIVVGPKIVDSTYNRRLWVKRNEVLRGTIYDRNMKALTKSERVNSELQKRE
YTGNSMFAHVLGYINVKYGLTGLEKKYDKELMSTDIKDDLTTFFKNKGKLEQKVGHNLKTTLDYTVQKAAYDALGDNKGS
VVILNPKTGEVLGMVSKPSYDPNKLDEIWGSINKDKNIPLINRATAGLYPPGSTFKIVTALSALENMSGVMNRTFQDNGS
LDLGGGYSLSNYGGASYGSLDLKGAFVHSSNVVFGSLALEVGNSRLKGTTEKFYFNKEVPTDGIAIESSKFPTLKSYEKG
SIAQSGIGQSSVLVTPMEMALVGATVANDGVMMKPYLVKEVLNSKGELIRTIPPESNGEIVSKNNARIVKDFMKGVVEEG
SGRNASIEGIQVAGKTGTADYSESISGRAAAHSWFVGFAPYDNPQVAIAVIVENGGQGGIAAASIASQVMSTALSK
>Mature_476_residues
MKDISNNIKKVLLVFLICFMGLITYITYFEIVVGPKIVDSTYNRRLWVKRNEVLRGTIYDRNMKALTKSERVNSELQKRE
YTGNSMFAHVLGYINVKYGLTGLEKKYDKELMSTDIKDDLTTFFKNKGKLEQKVGHNLKTTLDYTVQKAAYDALGDNKGS
VVILNPKTGEVLGMVSKPSYDPNKLDEIWGSINKDKNIPLINRATAGLYPPGSTFKIVTALSALENMSGVMNRTFQDNGS
LDLGGGYSLSNYGGASYGSLDLKGAFVHSSNVVFGSLALEVGNSRLKGTTEKFYFNKEVPTDGIAIESSKFPTLKSYEKG
SIAQSGIGQSSVLVTPMEMALVGATVANDGVMMKPYLVKEVLNSKGELIRTIPPESNGEIVSKNNARIVKDFMKGVVEEG
SGRNASIEGIQVAGKTGTADYSESISGRAAAHSWFVGFAPYDNPQVAIAVIVENGGQGGIAAASIASQVMSTALSK

Specific function: Cell wall formation. Plays an important role in cell division and cell shape maintenance by cross-linking adjacent peptidoglycan chains through transpeptidation [H]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell membrane; Single-pass type II membrane protein (Probable). Note=Localized to the division site (septum) along with newly synthesized peptidoglycan, between segregated nucleoids (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786854, Length=452, Percent_Identity=28.3185840707965, Blast_Score=158, Evalue=8e-40,
Organism=Escherichia coli, GI1786272, Length=412, Percent_Identity=27.4271844660194, Blast_Score=114, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 51563; Mature: 51563

Theoretical pI: Translated: 9.52; Mature: 9.52

Prosite motif: PS00337 BETA_LACTAMASE_D

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDISNNIKKVLLVFLICFMGLITYITYFEIVVGPKIVDSTYNRRLWVKRNEVLRGTIYD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCEEEEEEEC
RNMKALTKSERVNSELQKREYTGNSMFAHVLGYINVKYGLTGLEKKYDKELMSTDIKDDL
CCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHEEEEECCCHHHHHHHHHHHHCCHHHHH
TTFFKNKGKLEQKVGHNLKTTLDYTVQKAAYDALGDNKGSVVILNPKTGEVLGMVSKPSY
HHHHHCCCCHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCEEEECCCCCC
DPNKLDEIWGSINKDKNIPLINRATAGLYPPGSTFKIVTALSALENMSGVMNRTFQDNGS
CHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
LDLGGGYSLSNYGGASYGSLDLKGAFVHSSNVVFGSLALEVGNSRLKGTTEKFYFNKEVP
EECCCCCEECCCCCCCCCCEEEEEEEEECCCEEEEEEEEEECCCCCCCCHHHEEECCCCC
TDGIAIESSKFPTLKSYEKGSIAQSGIGQSSVLVTPMEMALVGATVANDGVMMKPYLVKE
CCCEEEECCCCCCCCCCCCCCCHHCCCCCCCEEECHHHHHHHHHEECCCCEEECHHHHHH
VLNSKGELIRTIPPESNGEIVSKNNARIVKDFMKGVVEEGSGRNASIEGIQVAGKTGTAD
HHCCCCCEEEECCCCCCCCEEECCCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCC
YSESISGRAAAHSWFVGFAPYDNPQVAIAVIVENGGQGGIAAASIASQVMSTALSK
HHHHCCCCHHHCEEEEEECCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKDISNNIKKVLLVFLICFMGLITYITYFEIVVGPKIVDSTYNRRLWVKRNEVLRGTIYD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCEEEEEEEC
RNMKALTKSERVNSELQKREYTGNSMFAHVLGYINVKYGLTGLEKKYDKELMSTDIKDDL
CCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHEEEEECCCHHHHHHHHHHHHCCHHHHH
TTFFKNKGKLEQKVGHNLKTTLDYTVQKAAYDALGDNKGSVVILNPKTGEVLGMVSKPSY
HHHHHCCCCHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCEEEECCCCCC
DPNKLDEIWGSINKDKNIPLINRATAGLYPPGSTFKIVTALSALENMSGVMNRTFQDNGS
CHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
LDLGGGYSLSNYGGASYGSLDLKGAFVHSSNVVFGSLALEVGNSRLKGTTEKFYFNKEVP
EECCCCCEECCCCCCCCCCEEEEEEEEECCCEEEEEEEEEECCCCCCCCHHHEEECCCCC
TDGIAIESSKFPTLKSYEKGSIAQSGIGQSSVLVTPMEMALVGATVANDGVMMKPYLVKE
CCCEEEECCCCCCCCCCCCCCCHHCCCCCCCEEECHHHHHHHHHEECCCCEEECHHHHHH
VLNSKGELIRTIPPESNGEIVSKNNARIVKDFMKGVVEEGSGRNASIEGIQVAGKTGTAD
HHCCCCCEEEECCCCCCCCEEECCCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCC
YSESISGRAAAHSWFVGFAPYDNPQVAIAVIVENGGQGGIAAASIASQVMSTALSK
HHHHCCCCHHHCEEEEEECCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA