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The map label for this gene is lysC [H]

Identifier: 226950771

GI number: 226950771

Start: 3815825

End: 3817027

Strand: Reverse

Name: lysC [H]

Synonym: CLM_3781

Alternate gene names: 226950771

Gene position: 3817027-3815825 (Counterclockwise)

Preceding gene: 226950772

Following gene: 226950770

Centisome position: 91.86

GC content: 29.43

Gene sequence:

>1203_bases
ATGGCAACAATCGTTCAAAAATATGGAGGTACATCTGTAGCAACAGTAGAAAAGATAAAAAACGTAGCCAAATCCATCGT
AAAAAGAAAAAGGGAAGGAAATGATATAGTAGTTGTTGTTTCTGCTATGGGACATACTACAGATAATCTTATAAATTTAG
CTAAAGATATAAGCAATGTACCAGATAAAAGAGAATTAGATGCATTAATTTCTACAGGGGAAATGGTATCATCTTCACTT
TTATCTATGGCAATTAAGGAACTAGGGGAAGATGCTATAAGTTATACTGCTTATCAAATCAATATAAGCACTAATGGACA
ACATGGAAAATCTTTAATAAATGATATAGATAAAAAAAAGATAGATAAAAGCTTAAAGGAAGGAAAGGTTGTTGTTGTCG
CAGGATTTCAAGGAATAAATTGTGATGGTAACATAACAACTTTAGGTAGAGGGGGGTCTGATACCTCAGCAGTAGCAATA
GCAGCAAAGCTAAATAATGCTATATGTGAAATATACACAGATGTAGATGGAATATATAGTGTAGATCCAAGAGTATATCA
TAGAGCTAAAAAATTAGATTATATAGATTATGAAGAAATGCTAGAATTATCTAGCTTAGGTGCACAGGTGATGCACTCAA
GATCTATAGAATTAGCTGAAAAATATAATATACCCATTTATGTAGGATTAAGTAATAGTAATATCAAGGGAACAGTTATT
AGGGGGATGAATAATATGAATATGGAAATGAAATCAGTAACAGGATTAGCAACTAGTGATGATGATGTTGCAATAACAAT
GGAAGATATAAATGAAAATATAAACATAGTAGCAGATATATTTAGTAGATTAGCAGAAAAGCAGATTAATGTAGATATGA
TAAGCCAAACAGCACCTATAAATAGTAGAATAAGTCTATCCTTTACTATACCAAAAGAAGATTTGCAGGAAGGCATGAAG
ATAATAAAAAATTATGCCTCAAAGGATAAAATAAAAATTGATGAAAACTTAACTAAATTTTCTATAGTGGGAATAGGAAT
GAAAAATACATCCGGTGTAGCAGCTAAAATGTTAAAAATTTTTAGTGATAATAATATAAAGGTAAAAATGATAACAACCT
CAGAAATAAGAATCACTTGTGCAATAAATGTAAGGGATAAAATAAATGCTATAAATTTAGTAGTAAAAGAATTTAATTTA
TAA

Upstream 100 bases:

>100_bases
TGCATTATTTTTTATTTTTATAAAATACATAAGTTAGAGTATTTATTAAATTTTAGAGAAACATTTAAAATATTTTATTT
ATAAATAGGGGGGTATTTAT

Downstream 100 bases:

>100_bases
ATAGGATGTGGGAGTATGAAACTTTTTGGAAGTATGAATATAAAGAACAATAATTTATATATAGGTGAAATAAATACGTT
AGACTTGGCTAAGAAATTTA

Product: aspartokinase II

Products: NA

Alternate protein names: Aspartate kinase 2; Aspartokinase II [H]

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MATIVQKYGGTSVATVEKIKNVAKSIVKRKREGNDIVVVVSAMGHTTDNLINLAKDISNVPDKRELDALISTGEMVSSSL
LSMAIKELGEDAISYTAYQINISTNGQHGKSLINDIDKKKIDKSLKEGKVVVVAGFQGINCDGNITTLGRGGSDTSAVAI
AAKLNNAICEIYTDVDGIYSVDPRVYHRAKKLDYIDYEEMLELSSLGAQVMHSRSIELAEKYNIPIYVGLSNSNIKGTVI
RGMNNMNMEMKSVTGLATSDDDVAITMEDINENINIVADIFSRLAEKQINVDMISQTAPINSRISLSFTIPKEDLQEGMK
IIKNYASKDKIKIDENLTKFSIVGIGMKNTSGVAAKMLKIFSDNNIKVKMITTSEIRITCAINVRDKINAINLVVKEFNL

Sequences:

>Translated_400_residues
MATIVQKYGGTSVATVEKIKNVAKSIVKRKREGNDIVVVVSAMGHTTDNLINLAKDISNVPDKRELDALISTGEMVSSSL
LSMAIKELGEDAISYTAYQINISTNGQHGKSLINDIDKKKIDKSLKEGKVVVVAGFQGINCDGNITTLGRGGSDTSAVAI
AAKLNNAICEIYTDVDGIYSVDPRVYHRAKKLDYIDYEEMLELSSLGAQVMHSRSIELAEKYNIPIYVGLSNSNIKGTVI
RGMNNMNMEMKSVTGLATSDDDVAITMEDINENINIVADIFSRLAEKQINVDMISQTAPINSRISLSFTIPKEDLQEGMK
IIKNYASKDKIKIDENLTKFSIVGIGMKNTSGVAAKMLKIFSDNNIKVKMITTSEIRITCAINVRDKINAINLVVKEFNL
>Mature_399_residues
ATIVQKYGGTSVATVEKIKNVAKSIVKRKREGNDIVVVVSAMGHTTDNLINLAKDISNVPDKRELDALISTGEMVSSSLL
SMAIKELGEDAISYTAYQINISTNGQHGKSLINDIDKKKIDKSLKEGKVVVVAGFQGINCDGNITTLGRGGSDTSAVAIA
AKLNNAICEIYTDVDGIYSVDPRVYHRAKKLDYIDYEEMLELSSLGAQVMHSRSIELAEKYNIPIYVGLSNSNIKGTVIR
GMNNMNMEMKSVTGLATSDDDVAITMEDINENINIVADIFSRLAEKQINVDMISQTAPINSRISLSFTIPKEDLQEGMKI
IKNYASKDKIKIDENLTKFSIVGIGMKNTSGVAAKMLKIFSDNNIKVKMITTSEIRITCAINVRDKINAINLVVKEFNL

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=468, Percent_Identity=26.0683760683761, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI1790455, Length=372, Percent_Identity=25.2688172043011, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1790376, Length=405, Percent_Identity=25.9259259259259, Blast_Score=88, Evalue=9e-19,
Organism=Saccharomyces cerevisiae, GI6320893, Length=299, Percent_Identity=28.4280936454849, Blast_Score=104, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR005260
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 43742; Mature: 43611

Theoretical pI: Translated: 7.49; Mature: 7.49

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATIVQKYGGTSVATVEKIKNVAKSIVKRKREGNDIVVVVSAMGHTTDNLINLAKDISNV
CCCEEHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHCC
PDKRELDALISTGEMVSSSLLSMAIKELGEDAISYTAYQINISTNGQHGKSLINDIDKKK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEEEEEEEECCCCHHHHHHHHHHHHH
IDKSLKEGKVVVVAGFQGINCDGNITTLGRGGSDTSAVAIAAKLNNAICEIYTDVDGIYS
HHHHHHCCCEEEEECCCCCCCCCCEEEECCCCCCCCCEEEEEHHCCCEEEEEECCCCEEE
VDPRVYHRAKKLDYIDYEEMLELSSLGAQVMHSRSIELAEKYNIPIYVGLSNSNIKGTVI
CCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHCCCEEEEEECCCCCCEEEE
RGMNNMNMEMKSVTGLATSDDDVAITMEDINENINIVADIFSRLAEKQINVDMISQTAPI
ECCCCCCEEEHHHCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEECCCCCC
NSRISLSFTIPKEDLQEGMKIIKNYASKDKIKIDENLTKFSIVGIGMKNTSGVAAKMLKI
CCEEEEEEECCHHHHHHHHHHHHHHCCCCCEEECCCCEEEEEEEECCCCCCHHHHHHHHH
FSDNNIKVKMITTSEIRITCAINVRDKINAINLVVKEFNL
HCCCCEEEEEEEECEEEEEEEEECCCCCCCEEEEEEECCC
>Mature Secondary Structure 
ATIVQKYGGTSVATVEKIKNVAKSIVKRKREGNDIVVVVSAMGHTTDNLINLAKDISNV
CCEEHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHCC
PDKRELDALISTGEMVSSSLLSMAIKELGEDAISYTAYQINISTNGQHGKSLINDIDKKK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEEEEEEEECCCCHHHHHHHHHHHHH
IDKSLKEGKVVVVAGFQGINCDGNITTLGRGGSDTSAVAIAAKLNNAICEIYTDVDGIYS
HHHHHHCCCEEEEECCCCCCCCCCEEEECCCCCCCCCEEEEEHHCCCEEEEEECCCCEEE
VDPRVYHRAKKLDYIDYEEMLELSSLGAQVMHSRSIELAEKYNIPIYVGLSNSNIKGTVI
CCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHCCCEEEEEECCCCCCEEEE
RGMNNMNMEMKSVTGLATSDDDVAITMEDINENINIVADIFSRLAEKQINVDMISQTAPI
ECCCCCCEEEHHHCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEECCCCCC
NSRISLSFTIPKEDLQEGMKIIKNYASKDKIKIDENLTKFSIVGIGMKNTSGVAAKMLKI
CCEEEEEEECCHHHHHHHHHHHHHHCCCCCEEECCCCEEEEEEEECCCCCCHHHHHHHHH
FSDNNIKVKMITTSEIRITCAINVRDKINAINLVVKEFNL
HCCCCEEEEEEEECEEEEEEEEECCCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1444390 [H]