| Definition | Clostridium botulinum A2 str. Kyoto chromosome, complete genome. |
|---|---|
| Accession | NC_012563 |
| Length | 4,155,278 |
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The map label for this gene is ligA [H]
Identifier: 226950614
GI number: 226950614
Start: 3634977
End: 3635705
Strand: Reverse
Name: ligA [H]
Synonym: CLM_3598
Alternate gene names: 226950614
Gene position: 3635705-3634977 (Counterclockwise)
Preceding gene: 226950615
Following gene: 226950613
Centisome position: 87.5
GC content: 22.36
Gene sequence:
>729_bases ATGGATAATATAAACTTCATAAATAGAATTAAAATCATGGTAGGAGAGAAAGGAATAAATATTAAAGAGTTAAATGATGA AACCATAAATATTTTATTTAAAAATGGGCTTTTAAATAATGCTTATGATATCTTTTTATTAAAAAAAGAAGAACTATATA AAATAGATGGTTTTACAAAAGAATATGTAGATGAACTTATAAAATCTATTAATAAAACTAAAAATTGTAGCTTTGAAAAA TTTATATATGCATGTTCTATACCTAAAGTTACAGAAAAAGAAGCCATAGTTATTGCTCACACTTTTTTAAATTTTACAGA TTTAGTAATAGATATAAACAATAACGATTGTGATAGATTAAAAAGAATAGATGGAATGAGTGAAGAAATAGTAGAAAGTA TAAAAAGAAATAAGGTTCTTTTAGTTAATTTATTTATGTATGTTAATCCAATTTCTATAGATGAAAAGAATACAAATATT AAGAGATATAAATTCGCTATAACAGGAGTACTAAATAAGGATACAAGTTATTATGAGGAAATGATAAAGGAAGCTAATTG TATAGTGGTAGATAATGTAACAAAGGATGTAGATTATTTGGTATTTGGTGATTTAGCTAATGCTATTAAAATGATGGACG CTAAAAAATATAATACTAGGTTAATATCAGAACGTCAATTAGTAGATATATTAAAGGAAATTAAAGAGAATAATAAAATG AAAAATTAA
Upstream 100 bases:
>100_bases TTTGAGTAATTTAGCAAAAGAAGATTTAATAAAAAAAATTTTGAATTTAGAAAAAGAAATTTAAGGTGTAAATATATATA ACAAAGATGGGGAGATATAG
Downstream 100 bases:
>100_bases AAATTGTAAAACAAATAAATAGCAAAAACTATTGATATATCAAGTTATCAATAGTTTTTTTGTTCCAATAAGCTTAGTTA TAAGTAAAAAATTTAAAATC
Product: BRCT domain-containing protein
Products: NA
Alternate protein names: Polydeoxyribonucleotide synthase [NAD+] [H]
Number of amino acids: Translated: 242; Mature: 242
Protein sequence:
>242_residues MDNINFINRIKIMVGEKGINIKELNDETINILFKNGLLNNAYDIFLLKKEELYKIDGFTKEYVDELIKSINKTKNCSFEK FIYACSIPKVTEKEAIVIAHTFLNFTDLVIDINNNDCDRLKRIDGMSEEIVESIKRNKVLLVNLFMYVNPISIDEKNTNI KRYKFAITGVLNKDTSYYEEMIKEANCIVVDNVTKDVDYLVFGDLANAIKMMDAKKYNTRLISERQLVDILKEIKENNKM KN
Sequences:
>Translated_242_residues MDNINFINRIKIMVGEKGINIKELNDETINILFKNGLLNNAYDIFLLKKEELYKIDGFTKEYVDELIKSINKTKNCSFEK FIYACSIPKVTEKEAIVIAHTFLNFTDLVIDINNNDCDRLKRIDGMSEEIVESIKRNKVLLVNLFMYVNPISIDEKNTNI KRYKFAITGVLNKDTSYYEEMIKEANCIVVDNVTKDVDYLVFGDLANAIKMMDAKKYNTRLISERQLVDILKEIKENNKM KN >Mature_242_residues MDNINFINRIKIMVGEKGINIKELNDETINILFKNGLLNNAYDIFLLKKEELYKIDGFTKEYVDELIKSINKTKNCSFEK FIYACSIPKVTEKEAIVIAHTFLNFTDLVIDINNNDCDRLKRIDGMSEEIVESIKRNKVLLVNLFMYVNPISIDEKNTNI KRYKFAITGVLNKDTSYYEEMIKEANCIVVDNVTKDVDYLVFGDLANAIKMMDAKKYNTRLISERQLVDILKEIKENNKM KN
Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam
COG id: COG0272
COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)
Gene ontology:
Cell location: Cytoplasmic
Metaboloic importance: NA
Operon status: Not Known
Operon components: None
Similarity: Contains 1 BRCT domain [H]
Homologues:
None
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR001357 - InterPro: IPR018239 - InterPro: IPR004150 - InterPro: IPR001679 - InterPro: IPR013839 - InterPro: IPR013840 - InterPro: IPR003583 - InterPro: IPR012340 - InterPro: IPR016027 - InterPro: IPR010994 - InterPro: IPR004149 [H]
Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD [H]
EC number: =6.5.1.2 [H]
Molecular weight: Translated: 28183; Mature: 28183
Theoretical pI: Translated: 6.11; Mature: 6.11
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.7 %Cys (Translated Protein) 3.3 %Met (Translated Protein) 5.0 %Cys+Met (Translated Protein) 1.7 %Cys (Mature Protein) 3.3 %Met (Mature Protein) 5.0 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MDNINFINRIKIMVGEKGINIKELNDETINILFKNGLLNNAYDIFLLKKEELYKIDGFTK CCCCCCEEEEEEEECCCCCCEEECCCCEEEEEEECCCCCCCEEEEEECHHHHEEECCCCH EYVDELIKSINKTKNCSFEKFIYACSIPKVTEKEAIVIAHTFLNFTDLVIDINNNDCDRL HHHHHHHHHHCCCCCCCHHHEEEEECCCCCCCCCEEEEEEEHHCEEEEEEECCCCCHHHH KRIDGMSEEIVESIKRNKVLLVNLFMYVNPISIDEKNTNIKRYKFAITGVLNKDTSYYEE HHHCCCHHHHHHHHHCCCEEEEEEEHHCCCCCCCCCCCCCEEEEEEEEEEECCCHHHHHH MIKEANCIVVDNVTKDVDYLVFGDLANAIKMMDAKKYNTRLISERQLVDILKEIKENNKM HHHCCCEEEEECCCCCCHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC KN CC >Mature Secondary Structure MDNINFINRIKIMVGEKGINIKELNDETINILFKNGLLNNAYDIFLLKKEELYKIDGFTK CCCCCCEEEEEEEECCCCCCEEECCCCEEEEEEECCCCCCCEEEEEECHHHHEEECCCCH EYVDELIKSINKTKNCSFEKFIYACSIPKVTEKEAIVIAHTFLNFTDLVIDINNNDCDRL HHHHHHHHHHCCCCCCCHHHEEEEECCCCCCCCCEEEEEEEHHCEEEEEEECCCCCHHHH KRIDGMSEEIVESIKRNKVLLVNLFMYVNPISIDEKNTNIKRYKFAITGVLNKDTSYYEE HHHCCCHHHHHHHHHCCCEEEEEEEHHCCCCCCCCCCCCCEEEEEEEEEEECCCHHHHHH MIKEANCIVVDNVTKDVDYLVFGDLANAIKMMDAKKYNTRLISERQLVDILKEIKENNKM HHHCCCEEEEECCCCCCHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC KN CC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 10.0
TargetDB status: NA
Availability: NA
References: NA