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The map label for this gene is glpK [H]

Identifier: 226950196

GI number: 226950196

Start: 3160658

End: 3162154

Strand: Reverse

Name: glpK [H]

Synonym: CLM_3150

Alternate gene names: 226950196

Gene position: 3162154-3160658 (Counterclockwise)

Preceding gene: 226950197

Following gene: 226950194

Centisome position: 76.1

GC content: 33.67

Gene sequence:

>1497_bases
ATGGAAAAGTATATAATGTCTTTAGATCAAGGAACTACTAGTTCAAGATGTATAATATTTAATAAAAAAGGTGAAGTAGT
AAGTGTTGCCCAAAAGGAATTTACACAAATATATCCTAAAGCAGGATGGGTAGAGCATGATCCACTAGAAATATGGGGAA
AACAAGCAGGGGTAGCAGGAGAAGCATTAAATATAGCTAGAATTTCTCCAGAACAAATAGCAGGTATAGGTATAACTAAT
CAAAGGGAAACCACAGTAGTTTGGAATAAAAGAACAGGAATGCCAATATATAATGCTATAGTATGGCAATGTAGAAGAAC
AGCTGGTTATTGTGATGAATTAAGAGAAAAAGGTTTAGATAAAACTATAAAAGAAAAAACAGGTTTAATGCTAGATGCCT
ATTTTTCAGCAACAAAAATAAAATGGATATTAGATAATGTAGAGGGAGCTAGGGAATTAGCAGAAAAAGGTGATTTGTTA
TTCGGAAATATAGATACTTGGTTAATCTGGAATATGACAAAAGGGAAAATTCATGTAACAGATTATACTAATGCTTCAAG
AACTATGTTATTTAACATACATGAATTAAAATGGGATGAGGAATTATTAGAAATACTTGATATTCCTAAATCTATGCTTC
CGGAAGTAAAACCATCTAGTTGTGTATATGGTGAAACAGATGAAATATTGTTCGGGGTATCAATTCCAATATCCGGAGAT
GCAGGAGATCAACAAGCAGCATTGTTTGGACAAACTTGCTTTAATGCGGGTATGGCTAAAAATACTTATGGTACAGGTTG
TTTCTTGTTGATGAATACAGGAGAAAAAGCAGTAGATTCTAAAAATGGATTATTAACTACTATAGCTGTGGGTATAGATG
GAAAAGTTGAATATGCTTTAGAAGGAAGTATATTTATAGGTGGAGCAGTAATTCAATGGTTAAGAGATGAACTTAGAATG
GTAAAAACTGCTCAAGAAACAGAAAAATATGCTACAGAAGTAGAAGATAATAATGGAGTTTACCTTGTGCCAGCTTTTGT
TGGAATAGGTGCACCATACTGGGATTCTTATGCAAGAGGAACTATATTAGGACTTACAAGAGGAGCTAAAAAAGAACATA
TAATAAGAGCCGCATTAGAATCTATGGCATACCAAACTCATGATGTTTTAAAGGCTATGGAAGAGGACTCAGGTATAGAA
CTAAAAGCATTAAAAGTAGATGGGGGAGCTTGTCAAAATAATTTCTTAATGCAATTTCAATCTGATATATTAGGTGTAGA
AGTAGATAGACCAGAAGTAGTAGAGACTACAGCATTAGGAGCAGCTTATCTTGCAGGACTTGCAGTAGGTTATTGGAAGG
ATAGAAATGAAATATCACAAAACTGGGCAATTTCAAGAAGCTTTGCTCCAGCGATGGAAGATGAAAAGAAAGAAAAACTT
ATAAAAGGATGGCATAAGGCAGTAACAAAAGCAATGGATTGGGAAGATAAAGAATAA

Upstream 100 bases:

>100_bases
AGTTTAAATATAAGAAAGTTATGTTTTAAAAGATTATTTTACTAGTTTTTTTATCTTAATATCTTATTAAATGAATAAAA
AAGGGAAGGTGGAGTTTATA

Downstream 100 bases:

>100_bases
TTTAAAGAAATTATAGCTTAAAAGGTAATTACCTTCAGATTATTAAAAAGGGATATGTGACGAGATCGACAACTCAATTC
GCTAAGATATTATAAATTTG

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK [H]

Number of amino acids: Translated: 498; Mature: 498

Protein sequence:

>498_residues
MEKYIMSLDQGTTSSRCIIFNKKGEVVSVAQKEFTQIYPKAGWVEHDPLEIWGKQAGVAGEALNIARISPEQIAGIGITN
QRETTVVWNKRTGMPIYNAIVWQCRRTAGYCDELREKGLDKTIKEKTGLMLDAYFSATKIKWILDNVEGARELAEKGDLL
FGNIDTWLIWNMTKGKIHVTDYTNASRTMLFNIHELKWDEELLEILDIPKSMLPEVKPSSCVYGETDEILFGVSIPISGD
AGDQQAALFGQTCFNAGMAKNTYGTGCFLLMNTGEKAVDSKNGLLTTIAVGIDGKVEYALEGSIFIGGAVIQWLRDELRM
VKTAQETEKYATEVEDNNGVYLVPAFVGIGAPYWDSYARGTILGLTRGAKKEHIIRAALESMAYQTHDVLKAMEEDSGIE
LKALKVDGGACQNNFLMQFQSDILGVEVDRPEVVETTALGAAYLAGLAVGYWKDRNEISQNWAISRSFAPAMEDEKKEKL
IKGWHKAVTKAMDWEDKE

Sequences:

>Translated_498_residues
MEKYIMSLDQGTTSSRCIIFNKKGEVVSVAQKEFTQIYPKAGWVEHDPLEIWGKQAGVAGEALNIARISPEQIAGIGITN
QRETTVVWNKRTGMPIYNAIVWQCRRTAGYCDELREKGLDKTIKEKTGLMLDAYFSATKIKWILDNVEGARELAEKGDLL
FGNIDTWLIWNMTKGKIHVTDYTNASRTMLFNIHELKWDEELLEILDIPKSMLPEVKPSSCVYGETDEILFGVSIPISGD
AGDQQAALFGQTCFNAGMAKNTYGTGCFLLMNTGEKAVDSKNGLLTTIAVGIDGKVEYALEGSIFIGGAVIQWLRDELRM
VKTAQETEKYATEVEDNNGVYLVPAFVGIGAPYWDSYARGTILGLTRGAKKEHIIRAALESMAYQTHDVLKAMEEDSGIE
LKALKVDGGACQNNFLMQFQSDILGVEVDRPEVVETTALGAAYLAGLAVGYWKDRNEISQNWAISRSFAPAMEDEKKEKL
IKGWHKAVTKAMDWEDKE
>Mature_498_residues
MEKYIMSLDQGTTSSRCIIFNKKGEVVSVAQKEFTQIYPKAGWVEHDPLEIWGKQAGVAGEALNIARISPEQIAGIGITN
QRETTVVWNKRTGMPIYNAIVWQCRRTAGYCDELREKGLDKTIKEKTGLMLDAYFSATKIKWILDNVEGARELAEKGDLL
FGNIDTWLIWNMTKGKIHVTDYTNASRTMLFNIHELKWDEELLEILDIPKSMLPEVKPSSCVYGETDEILFGVSIPISGD
AGDQQAALFGQTCFNAGMAKNTYGTGCFLLMNTGEKAVDSKNGLLTTIAVGIDGKVEYALEGSIFIGGAVIQWLRDELRM
VKTAQETEKYATEVEDNNGVYLVPAFVGIGAPYWDSYARGTILGLTRGAKKEHIIRAALESMAYQTHDVLKAMEEDSGIE
LKALKVDGGACQNNFLMQFQSDILGVEVDRPEVVETTALGAAYLAGLAVGYWKDRNEISQNWAISRSFAPAMEDEKKEKL
IKGWHKAVTKAMDWEDKE

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism [H]

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family [H]

Homologues:

Organism=Homo sapiens, GI189409120, Length=502, Percent_Identity=49.402390438247, Blast_Score=437, Evalue=1e-122,
Organism=Homo sapiens, GI4504007, Length=502, Percent_Identity=49.402390438247, Blast_Score=436, Evalue=1e-122,
Organism=Homo sapiens, GI42794763, Length=506, Percent_Identity=48.6166007905138, Blast_Score=436, Evalue=1e-122,
Organism=Homo sapiens, GI41393575, Length=499, Percent_Identity=47.6953907815631, Blast_Score=428, Evalue=1e-120,
Organism=Homo sapiens, GI88196792, Length=510, Percent_Identity=34.5098039215686, Blast_Score=340, Evalue=2e-93,
Organism=Escherichia coli, GI1790361, Length=498, Percent_Identity=64.2570281124498, Blast_Score=687, Evalue=0.0,
Organism=Escherichia coli, GI1787789, Length=480, Percent_Identity=24.5833333333333, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1789168, Length=498, Percent_Identity=23.6947791164659, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1789987, Length=482, Percent_Identity=23.6514522821577, Blast_Score=89, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17535599, Length=502, Percent_Identity=42.4302788844622, Blast_Score=398, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI71986140, Length=518, Percent_Identity=26.2548262548263, Blast_Score=190, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI71986147, Length=271, Percent_Identity=30.2583025830258, Blast_Score=126, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6321755, Length=554, Percent_Identity=37.5451263537906, Blast_Score=322, Evalue=8e-89,
Organism=Drosophila melanogaster, GI17864214, Length=512, Percent_Identity=41.9921875, Blast_Score=392, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24655555, Length=506, Percent_Identity=39.3280632411067, Blast_Score=372, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24655560, Length=506, Percent_Identity=39.3280632411067, Blast_Score=372, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24655570, Length=506, Percent_Identity=39.3280632411067, Blast_Score=372, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24655574, Length=506, Percent_Identity=39.3280632411067, Blast_Score=372, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24655565, Length=506, Percent_Identity=39.3280632411067, Blast_Score=372, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24654573, Length=441, Percent_Identity=43.7641723356009, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI19922058, Length=532, Percent_Identity=34.0225563909774, Blast_Score=293, Evalue=2e-79,
Organism=Drosophila melanogaster, GI24652871, Length=498, Percent_Identity=34.136546184739, Blast_Score=274, Evalue=9e-74,
Organism=Drosophila melanogaster, GI24656367, Length=514, Percent_Identity=27.6264591439689, Blast_Score=224, Evalue=1e-58,
Organism=Drosophila melanogaster, GI21357867, Length=530, Percent_Identity=26.9811320754717, Blast_Score=219, Evalue=5e-57,
Organism=Drosophila melanogaster, GI24656372, Length=349, Percent_Identity=28.3667621776504, Blast_Score=173, Evalue=3e-43,
Organism=Drosophila melanogaster, GI45552909, Length=290, Percent_Identity=26.8965517241379, Blast_Score=123, Evalue=3e-28,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999 [H]

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N [H]

EC number: =2.7.1.30 [H]

Molecular weight: Translated: 55366; Mature: 55366

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00933 FGGY_KINASES_1 ; PS00445 FGGY_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKYIMSLDQGTTSSRCIIFNKKGEVVSVAQKEFTQIYPKAGWVEHDPLEIWGKQAGVAG
CCCCEEEECCCCCCCEEEEECCCCCEEHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCCC
EALNIARISPEQIAGIGITNQRETTVVWNKRTGMPIYNAIVWQCRRTAGYCDELREKGLD
CCEEEEECCHHHHEECCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCHHHHHHHCCCC
KTIKEKTGLMLDAYFSATKIKWILDNVEGARELAEKGDLLFGNIDTWLIWNMTKGKIHVT
HHHHHHCCCEEEEECCHHEEEEEHHCHHHHHHHHHCCCEEEECCCEEEEEEECCCEEEEE
DYTNASRTMLFNIHELKWDEELLEILDIPKSMLPEVKPSSCVYGETDEILFGVSIPISGD
ECCCCCCEEEEEEHHHCCHHHHHHHHCCCHHHCCCCCCCCEECCCCCCEEEEEEEEECCC
AGDQQAALFGQTCFNAGMAKNTYGTGCFLLMNTGEKAVDSKNGLLTTIAVGIDGKVEYAL
CCCCHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCHHHCCCCCEEEEEEEECCCEEEEEE
EGSIFIGGAVIQWLRDELRMVKTAQETEKYATEVEDNNGVYLVPAFVGIGAPYWDSYARG
ECCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHCCCCHHHHHCCC
TILGLTRGAKKEHIIRAALESMAYQTHDVLKAMEEDSGIELKALKVDGGACQNNFLMQFQ
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHH
SDILGVEVDRPEVVETTALGAAYLAGLAVGYWKDRNEISQNWAISRSFAPAMEDEKKEKL
HCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCEECCCCCCCCCHHHHHHH
IKGWHKAVTKAMDWEDKE
HHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MEKYIMSLDQGTTSSRCIIFNKKGEVVSVAQKEFTQIYPKAGWVEHDPLEIWGKQAGVAG
CCCCEEEECCCCCCCEEEEECCCCCEEHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCCC
EALNIARISPEQIAGIGITNQRETTVVWNKRTGMPIYNAIVWQCRRTAGYCDELREKGLD
CCEEEEECCHHHHEECCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCHHHHHHHCCCC
KTIKEKTGLMLDAYFSATKIKWILDNVEGARELAEKGDLLFGNIDTWLIWNMTKGKIHVT
HHHHHHCCCEEEEECCHHEEEEEHHCHHHHHHHHHCCCEEEECCCEEEEEEECCCEEEEE
DYTNASRTMLFNIHELKWDEELLEILDIPKSMLPEVKPSSCVYGETDEILFGVSIPISGD
ECCCCCCEEEEEEHHHCCHHHHHHHHCCCHHHCCCCCCCCEECCCCCCEEEEEEEEECCC
AGDQQAALFGQTCFNAGMAKNTYGTGCFLLMNTGEKAVDSKNGLLTTIAVGIDGKVEYAL
CCCCHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCHHHCCCCCEEEEEEEECCCEEEEEE
EGSIFIGGAVIQWLRDELRMVKTAQETEKYATEVEDNNGVYLVPAFVGIGAPYWDSYARG
ECCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHCCCCHHHHHCCC
TILGLTRGAKKEHIIRAALESMAYQTHDVLKAMEEDSGIELKALKVDGGACQNNFLMQFQ
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHH
SDILGVEVDRPEVVETTALGAAYLAGLAVGYWKDRNEISQNWAISRSFAPAMEDEKKEKL
HCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCEECCCCCCCCCHHHHHHH
IKGWHKAVTKAMDWEDKE
HHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA