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The map label for this gene is recJ [H]

Identifier: 226950176

GI number: 226950176

Start: 3133792

End: 3135573

Strand: Reverse

Name: recJ [H]

Synonym: CLM_3130

Alternate gene names: 226950176

Gene position: 3135573-3133792 (Counterclockwise)

Preceding gene: 226950178

Following gene: 226950175

Centisome position: 75.46

GC content: 28.17

Gene sequence:

>1782_bases
TTGAATAAAAAGTGGATGTTAAGAAGAAATAAATTGAATATAAGAGATATAGCTAATAAATCAGGAATAAGTGAGACCTT
ATGTACTGTATTAGTTAATAGAGAAATATATAACTTAGAAGATATAAAGGATTTTTTAGAGCCTTCTTTAGAAAAACTTT
ATAATCCTTTATTGATGAAAGATATGGACAAAGGTACGGAAATAATAAAAAGGTCAATAATAGATAAAAAGAAAATAGCT
ATTTATGGAGATTATGATGCAGATGGGGTTACTAGCACCGTAATATTATATATAGCTTTAAAGGAATGCGAAGCTAATGT
GATTTATTATATACCAGATAGAGAAACAGAAGGGTACGGTATGTGTACAGAAAGAGTTGAAAGATTAAAAGCAGAGGGCG
TAGAGGTTATAATTACTTGTGATAATGGTATAGCTGCGTTAGAGCAAGTAGAACGAGCAAAAGAATTAGGTATGACGGTG
GTAATTACAGATCATCATGAATTACCTTTTATAGAAAATGAAAAGGAAGAAAGAGAATATGTAGTACCAAAAGCAGATGC
TATAATAAATCCAAAACAAAAGGATTGTTATTATCCCTTTAAAATGCTTTGTGGAGCAGGTATAGCTTTTAAGTTTTCAA
AACTTTTATATGAAAAATTAAATATGAATCCTAATAAATATAAAGAATTATTACAATTTGCAGCTATAGGAACAATATGT
GATGTAGTAGATTTAAAGGGTGAAAATAGAATAATAGCTAAATTGGGATTAGAAAGTATAAATAACACAAATAATCTAGG
CTTGAGAGCACTTATAAAAGAGACTTCATTAAATGAGAAAAATATAACTTCTTATAATGTAGGATTTATAATAGGACCTT
GCATAAATGCTACAGGGAGATTAGATACAGCGGCTCTTTCTGTGGAGCTGTTTTTGGCTAAAGATATAAAAAGGGCAGAA
AAACTAGCAAAAGAATTAAGAAACTTAAATACTGAGAGACAAGAAATAACGATGGTAGGTGTAGAAGAAATAACATATGC
CATAGAGAATTCATCATTAAAAAATGATAAAGTGTTAGTCATTTATAAAGAAAATATACATGAAAGTATAGCGGGTATAG
TATCAGGAAGAATAAAGGATGCATATAATCGACCAACAATAGTTTTAACTAAAGGCAAAGAAATGCCTAAAGGATCTGGA
AGATCTATAGATGGATATAATCTTTTTGAAGAACTTATGAAATGTAAGGAATACATATATAAATTTGGGGGTCATCCAAT
GGCTGCTGGTCTTACTATAGAAGAAAAAAACATAGATAAACTAAGAGAAGAATTAAATAAAAATTGCAATTTAAAAGATG
AAGACTTTATACCTAAAATAAGAATAGATAAGAGATTAGCTCTAAAAGAAGTGAACTTAGAACTAATAGATAGCCTACAG
TGTTTAGAACCTTTTGGTAAAGGAAATAGTTCACCAATACTAGCAGAAAAAAATATAAAAATAGAAGGAATAAGAATATT
AGGTAAAGATCAAAACACATTAAAATTAACTTGTAGAGTAGAAGATACAAATAAAAGAATACATGCAATAGCCTTTGGAA
AAGTGGATGAATTTAAAAGAGATTTAGAAAATATATATGAAGATGAAAATGTGGAAAATATTATAACTAACCCACTTATG
GCAGGTTTAAAATTAGATTTAATATATTATCCAATGATTAATGAGTATAATGGAAACGTGTATACTCAATTAAGAATAAT
AGATTACAGAATAAATGGATAA

Upstream 100 bases:

>100_bases
TTATAAGAATATGTAAAATAAGAATTAAAATTGAGATAAATAAAATAAATATAAAAATATTAATCTTAATGGTATAATTA
GCAAGGAAGAGGTGTGTGGC

Downstream 100 bases:

>100_bases
AATAAGGAAATTACGACTTAAAGTAAGTTATACCACAGCCTATTAAAAAGGGATATGTGGCTACAAAGGCGTCTCAATTC
GCTAAGAGATTCTAATTTGT

Product: single-stranded-DNA-specific exonuclease RecJ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 593; Mature: 593

Protein sequence:

>593_residues
MNKKWMLRRNKLNIRDIANKSGISETLCTVLVNREIYNLEDIKDFLEPSLEKLYNPLLMKDMDKGTEIIKRSIIDKKKIA
IYGDYDADGVTSTVILYIALKECEANVIYYIPDRETEGYGMCTERVERLKAEGVEVIITCDNGIAALEQVERAKELGMTV
VITDHHELPFIENEKEEREYVVPKADAIINPKQKDCYYPFKMLCGAGIAFKFSKLLYEKLNMNPNKYKELLQFAAIGTIC
DVVDLKGENRIIAKLGLESINNTNNLGLRALIKETSLNEKNITSYNVGFIIGPCINATGRLDTAALSVELFLAKDIKRAE
KLAKELRNLNTERQEITMVGVEEITYAIENSSLKNDKVLVIYKENIHESIAGIVSGRIKDAYNRPTIVLTKGKEMPKGSG
RSIDGYNLFEELMKCKEYIYKFGGHPMAAGLTIEEKNIDKLREELNKNCNLKDEDFIPKIRIDKRLALKEVNLELIDSLQ
CLEPFGKGNSSPILAEKNIKIEGIRILGKDQNTLKLTCRVEDTNKRIHAIAFGKVDEFKRDLENIYEDENVENIITNPLM
AGLKLDLIYYPMINEYNGNVYTQLRIIDYRING

Sequences:

>Translated_593_residues
MNKKWMLRRNKLNIRDIANKSGISETLCTVLVNREIYNLEDIKDFLEPSLEKLYNPLLMKDMDKGTEIIKRSIIDKKKIA
IYGDYDADGVTSTVILYIALKECEANVIYYIPDRETEGYGMCTERVERLKAEGVEVIITCDNGIAALEQVERAKELGMTV
VITDHHELPFIENEKEEREYVVPKADAIINPKQKDCYYPFKMLCGAGIAFKFSKLLYEKLNMNPNKYKELLQFAAIGTIC
DVVDLKGENRIIAKLGLESINNTNNLGLRALIKETSLNEKNITSYNVGFIIGPCINATGRLDTAALSVELFLAKDIKRAE
KLAKELRNLNTERQEITMVGVEEITYAIENSSLKNDKVLVIYKENIHESIAGIVSGRIKDAYNRPTIVLTKGKEMPKGSG
RSIDGYNLFEELMKCKEYIYKFGGHPMAAGLTIEEKNIDKLREELNKNCNLKDEDFIPKIRIDKRLALKEVNLELIDSLQ
CLEPFGKGNSSPILAEKNIKIEGIRILGKDQNTLKLTCRVEDTNKRIHAIAFGKVDEFKRDLENIYEDENVENIITNPLM
AGLKLDLIYYPMINEYNGNVYTQLRIIDYRING
>Mature_593_residues
MNKKWMLRRNKLNIRDIANKSGISETLCTVLVNREIYNLEDIKDFLEPSLEKLYNPLLMKDMDKGTEIIKRSIIDKKKIA
IYGDYDADGVTSTVILYIALKECEANVIYYIPDRETEGYGMCTERVERLKAEGVEVIITCDNGIAALEQVERAKELGMTV
VITDHHELPFIENEKEEREYVVPKADAIINPKQKDCYYPFKMLCGAGIAFKFSKLLYEKLNMNPNKYKELLQFAAIGTIC
DVVDLKGENRIIAKLGLESINNTNNLGLRALIKETSLNEKNITSYNVGFIIGPCINATGRLDTAALSVELFLAKDIKRAE
KLAKELRNLNTERQEITMVGVEEITYAIENSSLKNDKVLVIYKENIHESIAGIVSGRIKDAYNRPTIVLTKGKEMPKGSG
RSIDGYNLFEELMKCKEYIYKFGGHPMAAGLTIEEKNIDKLREELNKNCNLKDEDFIPKIRIDKRLALKEVNLELIDSLQ
CLEPFGKGNSSPILAEKNIKIEGIRILGKDQNTLKLTCRVEDTNKRIHAIAFGKVDEFKRDLENIYEDENVENIITNPLM
AGLKLDLIYYPMINEYNGNVYTQLRIIDYRING

Specific function: Putative single-stranded-DNA-specific exonuclease. RecA thread formation during DNA double-strand break repair requires RecJ or AadAB [H]

COG id: COG0608

COG function: function code L; Single-stranded DNA-specific exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OB DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789259, Length=614, Percent_Identity=30.4560260586319, Blast_Score=251, Evalue=1e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003156
- InterPro:   IPR001667
- InterPro:   IPR004610
- InterPro:   IPR018779 [H]

Pfam domain/function: PF01368 DHH; PF02272 DHHA1; PF10141 ssDNA-exonuc_C [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 67590; Mature: 67590

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKKWMLRRNKLNIRDIANKSGISETLCTVLVNREIYNLEDIKDFLEPSLEKLYNPLLMK
CCCCEEEECCCCCHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCHHHHH
DMDKGTEIIKRSIIDKKKIAIYGDYDADGVTSTVILYIALKECEANVIYYIPDRETEGYG
HCCHHHHHHHHHHCCCCEEEEEECCCCCCHHHHEEEEEEEECCCCCEEEEECCCCCCCCC
MCTERVERLKAEGVEVIITCDNGIAALEQVERAKELGMTVVITDHHELPFIENEKEEREY
HHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCEE
VVPKADAIINPKQKDCYYPFKMLCGAGIAFKFSKLLYEKLNMNPNKYKELLQFAAIGTIC
ECCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
DVVDLKGENRIIAKLGLESINNTNNLGLRALIKETSLNEKNITSYNVGFIIGPCINATGR
HHHCCCCCCEEEEEECHHHCCCCCCCCHHEEEHHCCCCCCCCEEEECCEEEECCCCCCCC
LDTAALSVELFLAKDIKRAEKLAKELRNLNTERQEITMVGVEEITYAIENSSLKNDKVLV
CCHHHEEEEHHHHHHHHHHHHHHHHHHCCCCCCHHEEEEEHHHHHEEECCCCCCCCCEEE
IYKENIHESIAGIVSGRIKDAYNRPTIVLTKGKEMPKGSGRSIDGYNLFEELMKCKEYIY
EECCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
KFGGHPMAAGLTIEEKNIDKLREELNKNCNLKDEDFIPKIRIDKRLALKEVNLELIDSLQ
HHCCCCEEECEEEECCCHHHHHHHHCCCCCCCCCCCCCCEEECCHHHHHHCCHHHHHHHH
CLEPFGKGNSSPILAEKNIKIEGIRILGKDQNTLKLTCRVEDTNKRIHAIAFGKVDEFKR
HHHHHCCCCCCCEEEECCEEEEEEEEEECCCCEEEEEEEEECCCCEEEEEEECCHHHHHH
DLENIYEDENVENIITNPLMAGLKLDLIYYPMINEYNGNVYTQLRIIDYRING
HHHHHHCCCCHHHHHHHHHHCCEEEEEEEEECEECCCCCEEEEEEEEEEEECC
>Mature Secondary Structure
MNKKWMLRRNKLNIRDIANKSGISETLCTVLVNREIYNLEDIKDFLEPSLEKLYNPLLMK
CCCCEEEECCCCCHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCHHHHH
DMDKGTEIIKRSIIDKKKIAIYGDYDADGVTSTVILYIALKECEANVIYYIPDRETEGYG
HCCHHHHHHHHHHCCCCEEEEEECCCCCCHHHHEEEEEEEECCCCCEEEEECCCCCCCCC
MCTERVERLKAEGVEVIITCDNGIAALEQVERAKELGMTVVITDHHELPFIENEKEEREY
HHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCEE
VVPKADAIINPKQKDCYYPFKMLCGAGIAFKFSKLLYEKLNMNPNKYKELLQFAAIGTIC
ECCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
DVVDLKGENRIIAKLGLESINNTNNLGLRALIKETSLNEKNITSYNVGFIIGPCINATGR
HHHCCCCCCEEEEEECHHHCCCCCCCCHHEEEHHCCCCCCCCEEEECCEEEECCCCCCCC
LDTAALSVELFLAKDIKRAEKLAKELRNLNTERQEITMVGVEEITYAIENSSLKNDKVLV
CCHHHEEEEHHHHHHHHHHHHHHHHHHCCCCCCHHEEEEEHHHHHEEECCCCCCCCCEEE
IYKENIHESIAGIVSGRIKDAYNRPTIVLTKGKEMPKGSGRSIDGYNLFEELMKCKEYIY
EECCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
KFGGHPMAAGLTIEEKNIDKLREELNKNCNLKDEDFIPKIRIDKRLALKEVNLELIDSLQ
HHCCCCEEECEEEECCCHHHHHHHHCCCCCCCCCCCCCCEEECCHHHHHHCCHHHHHHHH
CLEPFGKGNSSPILAEKNIKIEGIRILGKDQNTLKLTCRVEDTNKRIHAIAFGKVDEFKR
HHHHHCCCCCCCEEEECCEEEEEEEEEECCCCEEEEEEEEECCCCEEEEEEECCHHHHHH
DLENIYEDENVENIITNPLMAGLKLDLIYYPMINEYNGNVYTQLRIIDYRING
HHHHHHCCCCHHHHHHHHHHCCEEEEEEEEECEECCCCCEEEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]