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The map label for this gene is rnfC [H]

Identifier: 226949832

GI number: 226949832

Start: 2791178

End: 2792296

Strand: Reverse

Name: rnfC [H]

Synonym: CLM_2778

Alternate gene names: 226949832

Gene position: 2792296-2791178 (Counterclockwise)

Preceding gene: 226949833

Following gene: 226949831

Centisome position: 67.2

GC content: 33.33

Gene sequence:

>1119_bases
ATGGAGAAGTTATACAAATTCTCCCTTAAAATGCACGTAGGTGCACCAGATGTATCTTGTGTAAAAGAAGGACAAAAAGT
GAAGAGAGGTGAATGCATAGCAGAACCAAACGGACTTGGTGCTAAAATACATACAAGTGTATCCGGTGTAGTAGAAAAGA
TTACAGATAAGGAAATTATAATAAAAGCGGATGAGACTCAAACAAAAGAATTCGTTAAAATAAAAAAATGCGATAATTTA
GTAGATACGGTATTTGAAGCAGGGATAGTCGGAGCTGGTGGAGCAGGTTTCCCAACTCACATAAAGCTAAAGGCAGATAA
CAAAGATGGTTATATTATAGCAAACTGTGTAGAATGTGAACCAGCACTTCATCATAATATGAAAGTTATAGAAGAAACAC
CGGAATTAATAATAAATGGTATTAAGTATGCTATGAAGGCTACTAATTCAAAAAAAGGATACATAGCAATAAAATCAAAA
CATGAAAAAGCAGTAAGAGTATTAGAAGAAGCATTAAAGAATGTAAGTGATATAGAAATAAAATTGCTTAAAGACTTATA
CCCAATGGGTGAAGAAAGAGCTATAATCAATGCAATATTTGATAAATGGTTAGATGTAACTGAGCTTCCTATATCAGCAA
AATGTATTGTAATGAATGCTGAAACCTTAGCAAATATTACAAGAGCTGTTGAAGAAGGAAAACCAGTTATAGATAAAGAT
ATAACAGTAATAGGAAAATTAAAATCAGGCAATAAACCAAATATATTCCTACAAGTACCAGTTGGAACACCAGTAAAAGA
TTTAATAGAAAAATCTGGCGGAATAGATGGTGAGTATGGTGAACTAGTAATAGGTGGCCCATATACTGGAAAATCTGGAG
ATATAGAAAAAGATGTTGTTACTAAAATATCTGGTGGAGCAATAGTTACAATACCATTACCAGAATATAAAGGTCCACTA
GGACTACTAGTATGCGCCTGTGGAGCTAATGAAGAAAGATTAAAAGATGTAGCTACTAAAATGAATGCAAAAATAGCTGG
GGTAGTTGACTGCAAAAATATAGAATATCCAAAAGGTAAAGGAAATGGACCAGGAAAATGCAAAACACCTGGTGAATGA

Upstream 100 bases:

>100_bases
ATAGCTATAACCTAGTTTTATAGAAGTTGTTTATTTTTTTAACAAAGTTGGCATAGTTTATGCTTTAAAATATATATGAC
AGTGATTTGGAGGTGGGTTT

Downstream 100 bases:

>100_bases
CCAGGCCAAGCAGCTGCAGTTATGCAGCTTAAGAAAGATGGGGCAGAAAGAATATTAATATCAAATTGTAATGACTGTTC
AAATACAGTTATGCAAATAG

Product: putative electron transport protein

Products: NA

Alternate protein names: Nitrogen fixation protein rnfC [H]

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MEKLYKFSLKMHVGAPDVSCVKEGQKVKRGECIAEPNGLGAKIHTSVSGVVEKITDKEIIIKADETQTKEFVKIKKCDNL
VDTVFEAGIVGAGGAGFPTHIKLKADNKDGYIIANCVECEPALHHNMKVIEETPELIINGIKYAMKATNSKKGYIAIKSK
HEKAVRVLEEALKNVSDIEIKLLKDLYPMGEERAIINAIFDKWLDVTELPISAKCIVMNAETLANITRAVEEGKPVIDKD
ITVIGKLKSGNKPNIFLQVPVGTPVKDLIEKSGGIDGEYGELVIGGPYTGKSGDIEKDVVTKISGGAIVTIPLPEYKGPL
GLLVCACGANEERLKDVATKMNAKIAGVVDCKNIEYPKGKGNGPGKCKTPGE

Sequences:

>Translated_372_residues
MEKLYKFSLKMHVGAPDVSCVKEGQKVKRGECIAEPNGLGAKIHTSVSGVVEKITDKEIIIKADETQTKEFVKIKKCDNL
VDTVFEAGIVGAGGAGFPTHIKLKADNKDGYIIANCVECEPALHHNMKVIEETPELIINGIKYAMKATNSKKGYIAIKSK
HEKAVRVLEEALKNVSDIEIKLLKDLYPMGEERAIINAIFDKWLDVTELPISAKCIVMNAETLANITRAVEEGKPVIDKD
ITVIGKLKSGNKPNIFLQVPVGTPVKDLIEKSGGIDGEYGELVIGGPYTGKSGDIEKDVVTKISGGAIVTIPLPEYKGPL
GLLVCACGANEERLKDVATKMNAKIAGVVDCKNIEYPKGKGNGPGKCKTPGE
>Mature_372_residues
MEKLYKFSLKMHVGAPDVSCVKEGQKVKRGECIAEPNGLGAKIHTSVSGVVEKITDKEIIIKADETQTKEFVKIKKCDNL
VDTVFEAGIVGAGGAGFPTHIKLKADNKDGYIIANCVECEPALHHNMKVIEETPELIINGIKYAMKATNSKKGYIAIKSK
HEKAVRVLEEALKNVSDIEIKLLKDLYPMGEERAIINAIFDKWLDVTELPISAKCIVMNAETLANITRAVEEGKPVIDKD
ITVIGKLKSGNKPNIFLQVPVGTPVKDLIEKSGGIDGEYGELVIGGPYTGKSGDIEKDVVTKISGGAIVTIPLPEYKGPL
GLLVCACGANEERLKDVATKMNAKIAGVVDCKNIEYPKGKGNGPGKCKTPGE

Specific function: Required for nitrogen fixation. May be part of a membrane complex functioning as an intermediate in the electron transport to nitrogenase. Stabilizes rnfB [H]

COG id: COG4656

COG function: function code C; Predicted NADH:ubiquinone oxidoreductase, subunit RnfC

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Probable) [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787916, Length=309, Percent_Identity=24.2718446601942, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1788620, Length=233, Percent_Identity=24.4635193133047, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR010208
- InterPro:   IPR012285
- InterPro:   IPR009051
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10531 SLBB [H]

EC number: NA

Molecular weight: Translated: 40050; Mature: 40050

Theoretical pI: Translated: 7.89; Mature: 7.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKLYKFSLKMHVGAPDVSCVKEGQKVKRGECIAEPNGLGAKIHTSVSGVVEKITDKEII
CCCCEEEEEEEEECCCCHHHHHCCCHHHCCCCCCCCCCCCEEEEHHHHHHHHHHCCCEEE
IKADETQTKEFVKIKKCDNLVDTVFEAGIVGAGGAGFPTHIKLKADNKDGYIIANCVECE
EECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCEEEEEEECCC
PALHHNMKVIEETPELIINGIKYAMKATNSKKGYIAIKSKHEKAVRVLEEALKNVSDIEI
HHHHCCCHHHHHCHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHCCCHHHHH
KLLKDLYPMGEERAIINAIFDKWLDVTELPISAKCIVMNAETLANITRAVEEGKPVIDKD
HHHHHHCCCCCCHHHHHHHHHHHCCHHCCCCCEEEEEECHHHHHHHHHHHHCCCCCCCCC
ITVIGKLKSGNKPNIFLQVPVGTPVKDLIEKSGGIDGEYGELVIGGPYTGKSGDIEKDVV
EEEEEEECCCCCCEEEEEECCCCCHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCHHHH
TKISGGAIVTIPLPEYKGPLGLLVCACGANEERLKDVATKMNAKIAGVVDCKNIEYPKGK
HEECCCEEEEEECCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCC
GNGPGKCKTPGE
CCCCCCCCCCCC
>Mature Secondary Structure
MEKLYKFSLKMHVGAPDVSCVKEGQKVKRGECIAEPNGLGAKIHTSVSGVVEKITDKEII
CCCCEEEEEEEEECCCCHHHHHCCCHHHCCCCCCCCCCCCEEEEHHHHHHHHHHCCCEEE
IKADETQTKEFVKIKKCDNLVDTVFEAGIVGAGGAGFPTHIKLKADNKDGYIIANCVECE
EECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCEEEEEEECCC
PALHHNMKVIEETPELIINGIKYAMKATNSKKGYIAIKSKHEKAVRVLEEALKNVSDIEI
HHHHCCCHHHHHCHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHCCCHHHHH
KLLKDLYPMGEERAIINAIFDKWLDVTELPISAKCIVMNAETLANITRAVEEGKPVIDKD
HHHHHHCCCCCCHHHHHHHHHHHCCHHCCCCCEEEEEECHHHHHHHHHHHHCCCCCCCCC
ITVIGKLKSGNKPNIFLQVPVGTPVKDLIEKSGGIDGEYGELVIGGPYTGKSGDIEKDVV
EEEEEEECCCCCCEEEEEECCCCCHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCHHHH
TKISGGAIVTIPLPEYKGPLGLLVCACGANEERLKDVATKMNAKIAGVVDCKNIEYPKGK
HEECCCEEEEEECCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCC
GNGPGKCKTPGE
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: 4Fe-4S clusters [C]

Metal ions: Iron-Sulfur [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8264535; 9492268; 9154934 [H]