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The map label for this gene is lip2 [H]

Identifier: 226949454

GI number: 226949454

Start: 2461593

End: 2462624

Strand: Reverse

Name: lip2 [H]

Synonym: CLM_2386

Alternate gene names: 226949454

Gene position: 2462624-2461593 (Counterclockwise)

Preceding gene: 226949455

Following gene: 226949453

Centisome position: 59.26

GC content: 31.01

Gene sequence:

>1032_bases
GTGAAAAAAGTAATAAAAGTAGGATCAGTTATTCTAATTATACTTGTAATAGCAGGATTTTTTATTATTAAGAATTTAAC
TGAGACCAAGGATGATAAGCTTAATATGTATGTTGCTGCAAACTTGCAGTTATATAAAATTCTTAATCCTAAATCACTCA
ATAGTAAATCAATTGAAGAAACTAGAGGAGCTTTAAATAAGCAATCAACTAGGTGGTCAAATAAACCTATACCATTTTCC
AATATTAAAAACCTTCATATAAAAATGAACAATGAAAAAATACCAGTACGAATATATACACCTGAAAAGGGTAGTAATTT
CCCTATAATCATTTATTCACATGGTGGTTTCTGGATTGGAGGAAATGTTGATACTATTGATGGAGTTTGTAGAAAGCTTT
CACAGAATACAAAAGCAATTGTAATATCTGTAAACTATAGGCTTGCTCCAGAAAATCCTTTCCCCGCTGGTCTTAATGAT
GTATATAATGTGCTTCAGTGGACTTATAAAAATGGAAAAAGCATAAATGGAGATGAAAAACATATAGCAGTTGTAGGAGA
CAGTGCAGGTGGAAATCTTTCTGCTGCAGTTTCATCAATGTCACGGGATAAAAATGGACCTCCTATAACATGTCAGGTAT
TAATATATCCATCTACAAATATATCTGAACTGAACAGTAAATCTTGGTCTTATTTTTCTAATAGCTTTAATGTTTCAACA
GAGGATATGGAAAAGTATATTTCAATTTATGCACCAAAAAAAGAAGATAGAAAAGATCCTTATGGATCCCCACTTTTATC
TAAGGATCTTAGCAAATTACCTGATACACTCGTAGTGACAGCAGAAATTGATCCCCTTAGAGATGAAGGGGAAGCTTATG
CTAATAAACTAAAAGAATCTGGGGTTAAAGCAGAGGTTACTAGATATAAAGGTATTACTCATGGATTTATTACAATGGAT
AAGATTACAAATAAAGCAGACGAAGCATTAAATCAAATTTCTTTATATATACAAAAAGAGTTTCAAAAATAA

Upstream 100 bases:

>100_bases
CAAAGATTTTAAGTATATTAAAATGTGTAAAGGAAAAAATATATAAGTAAATTTAGTTATCTTTATTGCAGAATTTTTAT
AGTGGATGGAGGTAAATGAA

Downstream 100 bases:

>100_bases
ACTTAATATAATTATTTTATTACAAAACTTATTATAAAGGATGTTGCAGAGATAAAAGTTAAGTTTAGTTGACAAATTTC
CATCTTTACTTGATAGAAAA

Product: putative lipase/esterase

Products: NA

Alternate protein names: Triacylglycerol lipase [H]

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MKKVIKVGSVILIILVIAGFFIIKNLTETKDDKLNMYVAANLQLYKILNPKSLNSKSIEETRGALNKQSTRWSNKPIPFS
NIKNLHIKMNNEKIPVRIYTPEKGSNFPIIIYSHGGFWIGGNVDTIDGVCRKLSQNTKAIVISVNYRLAPENPFPAGLND
VYNVLQWTYKNGKSINGDEKHIAVVGDSAGGNLSAAVSSMSRDKNGPPITCQVLIYPSTNISELNSKSWSYFSNSFNVST
EDMEKYISIYAPKKEDRKDPYGSPLLSKDLSKLPDTLVVTAEIDPLRDEGEAYANKLKESGVKAEVTRYKGITHGFITMD
KITNKADEALNQISLYIQKEFQK

Sequences:

>Translated_343_residues
MKKVIKVGSVILIILVIAGFFIIKNLTETKDDKLNMYVAANLQLYKILNPKSLNSKSIEETRGALNKQSTRWSNKPIPFS
NIKNLHIKMNNEKIPVRIYTPEKGSNFPIIIYSHGGFWIGGNVDTIDGVCRKLSQNTKAIVISVNYRLAPENPFPAGLND
VYNVLQWTYKNGKSINGDEKHIAVVGDSAGGNLSAAVSSMSRDKNGPPITCQVLIYPSTNISELNSKSWSYFSNSFNVST
EDMEKYISIYAPKKEDRKDPYGSPLLSKDLSKLPDTLVVTAEIDPLRDEGEAYANKLKESGVKAEVTRYKGITHGFITMD
KITNKADEALNQISLYIQKEFQK
>Mature_343_residues
MKKVIKVGSVILIILVIAGFFIIKNLTETKDDKLNMYVAANLQLYKILNPKSLNSKSIEETRGALNKQSTRWSNKPIPFS
NIKNLHIKMNNEKIPVRIYTPEKGSNFPIIIYSHGGFWIGGNVDTIDGVCRKLSQNTKAIVISVNYRLAPENPFPAGLND
VYNVLQWTYKNGKSINGDEKHIAVVGDSAGGNLSAAVSSMSRDKNGPPITCQVLIYPSTNISELNSKSWSYFSNSFNVST
EDMEKYISIYAPKKEDRKDPYGSPLLSKDLSKLPDTLVVTAEIDPLRDEGEAYANKLKESGVKAEVTRYKGITHGFITMD
KITNKADEALNQISLYIQKEFQK

Specific function: Has An Esterase Activity. Triacetyl Glycerol (Triacetin) Is A Substrate Of The Enzyme. [C]

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI68299767, Length=304, Percent_Identity=27.6315789473684, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI157041239, Length=288, Percent_Identity=25.3472222222222, Blast_Score=92, Evalue=8e-19,
Organism=Homo sapiens, GI61966717, Length=289, Percent_Identity=25.6055363321799, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI206597554, Length=288, Percent_Identity=26.0416666666667, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI68051721, Length=148, Percent_Identity=29.7297297297297, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1786682, Length=288, Percent_Identity=25.6944444444444, Blast_Score=105, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17567059, Length=276, Percent_Identity=30.7971014492754, Blast_Score=122, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17540028, Length=270, Percent_Identity=29.2592592592593, Blast_Score=104, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI71996133, Length=276, Percent_Identity=27.8985507246377, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI72001146, Length=143, Percent_Identity=33.5664335664336, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI115533412, Length=89, Percent_Identity=34.8314606741573, Blast_Score=66, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI115533410, Length=89, Percent_Identity=34.8314606741573, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: =3.1.1.3 [H]

Molecular weight: Translated: 38277; Mature: 38277

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVIKVGSVILIILVIAGFFIIKNLTETKDDKLNMYVAANLQLYKILNPKSLNSKSIEE
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECEEEEEECCCCCCCCCHHHH
TRGALNKQSTRWSNKPIPFSNIKNLHIKMNNEKIPVRIYTPEKGSNFPIIIYSHGGFWIG
HHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEEEECCCCCCCCCEEEECCCCEEEC
GNVDTIDGVCRKLSQNTKAIVISVNYRLAPENPFPAGLNDVYNVLQWTYKNGKSINGDEK
CCCCHHHHHHHHHCCCCEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCC
HIAVVGDSAGGNLSAAVSSMSRDKNGPPITCQVLIYPSTNISELNSKSWSYFSNSFNVST
EEEEEECCCCCCHHHHHHHHHCCCCCCCEEEEEEEECCCCHHHHCCCCCHHCCCCCCCCH
EDMEKYISIYAPKKEDRKDPYGSPLLSKDLSKLPDTLVVTAEIDPLRDEGEAYANKLKES
HHHHHHHEEECCCCCCCCCCCCCCHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHC
GVKAEVTRYKGITHGFITMDKITNKADEALNQISLYIQKEFQK
CCEEEEHHHCCCCCCEEEHHHHCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKVIKVGSVILIILVIAGFFIIKNLTETKDDKLNMYVAANLQLYKILNPKSLNSKSIEE
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECEEEEEECCCCCCCCCHHHH
TRGALNKQSTRWSNKPIPFSNIKNLHIKMNNEKIPVRIYTPEKGSNFPIIIYSHGGFWIG
HHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEEEECCCCCCCCCEEEECCCCEEEC
GNVDTIDGVCRKLSQNTKAIVISVNYRLAPENPFPAGLNDVYNVLQWTYKNGKSINGDEK
CCCCHHHHHHHHHCCCCEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCC
HIAVVGDSAGGNLSAAVSSMSRDKNGPPITCQVLIYPSTNISELNSKSWSYFSNSFNVST
EEEEEECCCCCCHHHHHHHHHCCCCCCCEEEEEEEECCCCHHHHCCCCCHHCCCCCCCCH
EDMEKYISIYAPKKEDRKDPYGSPLLSKDLSKLPDTLVVTAEIDPLRDEGEAYANKLKES
HHHHHHHEEECCCCCCCCCCCCCCHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHC
GVKAEVTRYKGITHGFITMDKITNKADEALNQISLYIQKEFQK
CCEEEEHHHCCCCCCEEEHHHHCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1907455 [H]