Click here to switch to the map view.

The map label for this gene is truA [H]

Identifier: 226949212

GI number: 226949212

Start: 2181246

End: 2181986

Strand: Direct

Name: truA [H]

Synonym: CLM_2131

Alternate gene names: 226949212

Gene position: 2181246-2181986 (Clockwise)

Preceding gene: 226949211

Following gene: 226949215

Centisome position: 52.49

GC content: 29.28

Gene sequence:

>741_bases
ATGACTAATATAAAATTATATATACAATATGATGGTTCCAAATATAACGGTTGGCAAAAACAAAAAGAAAATGACAATAC
TATTCAAGGGAAAATTGAAAATGTACTTTCCCTTATGACAAGAGAAGATATAAACCTAATAGCTTCTGGCAGAACAGATA
CAGGCGTACATGCAAATATGCAGGTAGCCAATTTTCATACAACTTTTCAAGGTTCTACAGATGAAATATTAAACTATTGC
TATGAATATTTACCTAAAGATATTGTAGTTTATGATGCTTTTCATGTGGATGAAAGATTTCACTCTAGATTAAATGTTAA
AAAGAAAAAATATACTTATAAGATTTGTAATAAAAAATTTCATGATGTATTCTCTAGAAAATACTCTTATCATATACCAG
AAACTTTAGATATAAATGCTATGAAAGAAGCTTGTACCTATCTAGTAGGAAGACATGATTTTGCATCATTTACCGCAGCA
AGATCAAAAAAGAAATCTACTGTAAGAACGCTTTATAGCATAGATATTATAAAAAAAGATGGCTATGTAGACCTAGTATA
TCTAGGTAGTGGATTTTTGCATAAAATGATAAGAATATTAACAGGTACTCTTATAGAAGTAGGATTACACAATCTAAAAT
CTGTAGATGTAAAAAACATACTGACGGCAGAAGATAGAAGAAAAGCAGGACCTACTGCTCCAAGTCAAGGATTATTTATG
ACAAGTGTAGATTATGAATAG

Upstream 100 bases:

>100_bases
ATCCAAAAGAACCTAATTTAAAACAAATTTAGAACAGATTAAAAAGTAATATCTAAATAAAACTAATTAACTAAATATAT
TTGTAAAGGAAGTACTAAAT

Downstream 100 bases:

>100_bases
TTAATAAGCATTAAGAATATATGAAAATAAATTTATCTGATGACTCTCCGCTCTAATACTCCCATCTTCTTTAAAGTGGA
AGTAAGGAGCGGTTACGTCC

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I 1; tRNA-uridine isomerase I 1 [H]

Number of amino acids: Translated: 246; Mature: 245

Protein sequence:

>246_residues
MTNIKLYIQYDGSKYNGWQKQKENDNTIQGKIENVLSLMTREDINLIASGRTDTGVHANMQVANFHTTFQGSTDEILNYC
YEYLPKDIVVYDAFHVDERFHSRLNVKKKKYTYKICNKKFHDVFSRKYSYHIPETLDINAMKEACTYLVGRHDFASFTAA
RSKKKSTVRTLYSIDIIKKDGYVDLVYLGSGFLHKMIRILTGTLIEVGLHNLKSVDVKNILTAEDRRKAGPTAPSQGLFM
TSVDYE

Sequences:

>Translated_246_residues
MTNIKLYIQYDGSKYNGWQKQKENDNTIQGKIENVLSLMTREDINLIASGRTDTGVHANMQVANFHTTFQGSTDEILNYC
YEYLPKDIVVYDAFHVDERFHSRLNVKKKKYTYKICNKKFHDVFSRKYSYHIPETLDINAMKEACTYLVGRHDFASFTAA
RSKKKSTVRTLYSIDIIKKDGYVDLVYLGSGFLHKMIRILTGTLIEVGLHNLKSVDVKNILTAEDRRKAGPTAPSQGLFM
TSVDYE
>Mature_245_residues
TNIKLYIQYDGSKYNGWQKQKENDNTIQGKIENVLSLMTREDINLIASGRTDTGVHANMQVANFHTTFQGSTDEILNYCY
EYLPKDIVVYDAFHVDERFHSRLNVKKKKYTYKICNKKFHDVFSRKYSYHIPETLDINAMKEACTYLVGRHDFASFTAAR
SKKKSTVRTLYSIDIIKKDGYVDLVYLGSGFLHKMIRILTGTLIEVGLHNLKSVDVKNILTAEDRRKAGPTAPSQGLFMT
SVDYE

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=273, Percent_Identity=30.03663003663, Blast_Score=107, Evalue=1e-23,
Organism=Escherichia coli, GI1788657, Length=242, Percent_Identity=35.9504132231405, Blast_Score=157, Evalue=4e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 28324; Mature: 28192

Theoretical pI: Translated: 9.39; Mature: 9.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNIKLYIQYDGSKYNGWQKQKENDNTIQGKIENVLSLMTREDINLIASGRTDTGVHANM
CCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEEE
QVANFHTTFQGSTDEILNYCYEYLPKDIVVYDAFHVDERFHSRLNVKKKKYTYKICNKKF
EEEEEEEECCCCHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHCCHHHHHHHHHHHHHH
HDVFSRKYSYHIPETLDINAMKEACTYLVGRHDFASFTAARSKKKSTVRTLYSIDIIKKD
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEEEEEECC
GYVDLVYLGSGFLHKMIRILTGTLIEVGLHNLKSVDVKNILTAEDRRKAGPTAPSQGLFM
CCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHHCCCCCCCCCCEEE
TSVDYE
EECCCC
>Mature Secondary Structure 
TNIKLYIQYDGSKYNGWQKQKENDNTIQGKIENVLSLMTREDINLIASGRTDTGVHANM
CCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEEE
QVANFHTTFQGSTDEILNYCYEYLPKDIVVYDAFHVDERFHSRLNVKKKKYTYKICNKKF
EEEEEEEECCCCHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHCCHHHHHHHHHHHHHH
HDVFSRKYSYHIPETLDINAMKEACTYLVGRHDFASFTAARSKKKSTVRTLYSIDIIKKD
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEEEEEECC
GYVDLVYLGSGFLHKMIRILTGTLIEVGLHNLKSVDVKNILTAEDRRKAGPTAPSQGLFM
CCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHHCCCCCCCCCCEEE
TSVDYE
EECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12552129 [H]