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The map label for this gene is appB [H]

Identifier: 226948636

GI number: 226948636

Start: 1591708

End: 1592664

Strand: Direct

Name: appB [H]

Synonym: CLM_1531

Alternate gene names: 226948636

Gene position: 1591708-1592664 (Clockwise)

Preceding gene: 226948635

Following gene: 226948637

Centisome position: 38.31

GC content: 32.29

Gene sequence:

>957_bases
ATGACCTCAAGATATATACTAAAGAGATTAGGACAGGGTATATTGATTATATTTTTTCTAAGTATAATATCCTTTCTTAT
CATAAATGCTGCGCCGGGAGATCCGGCGGTAGCTATTTATGGTGGGAAAGCTGATAGATTAACCAACTCAGAAAGAGCTA
GAATAGTAAAAAATTATGGGTTAGATAGGCCGGTAATGGAGAGATACATAAAATGGTCTGGTCAAATGATAAGAGGAGAC
ATGGGCATATCCTACATAGAAGGAAGATCAGTATCAGAAATATTGAAGGAAAAGATTCCAAATACTATGGTGCTTTTTAT
AAATTCTATACTTTTGATAAGTTTAATTTCAATGATTTTGGGATTAAAAGCCGGATTTAATGAAGGCTCTATATGGGATA
AACTTCTTAGTACTTTAAGTATAGTATTTTATTCTATACCACCTTTTTGGTTAGCTCTCATTTTTATATTAGTATTTTCT
GTTTATACAGGTTGTTTGCCTTCCTCAGGAAATATGAGCATAGGTGGAGAAGGAAGTGTTTTAGATGTACTAAAGCATGC
CATACTACCTATAGCAGTTATAGTAATTACTCATGTAGGTGCTTATTCAAGATTTATACAAGAAAAAGTAAAGGAAGAGG
ATAAAAGCTATTATGCTATGGTAGCTAGAGCAAATGGAGTATTAGAAAAGAAAATAAGAAAAGGAATAACTAAAAATGCA
GTAGTTCCTTTTATAAATTATTTAGGCATAACTATTCCTACTTTTTTTAGTGGTTCTGTAATGATAGAAACGGTGTTTTC
CTGGCCGGGCCTTGGAATGCTTACAGTTAAAGCTGCTAACTCAAGAGATTATCCATTGCTTATGGGAGCTATATTTATAA
CTGGAGTTTTAGTTGTTTTATGTATGATAGTTACAGATATAATAGAAATAATAGTTAATCCACATCTTAGAAAGTAG

Upstream 100 bases:

>100_bases
CTATATATGCTGTAAACAAAAAGGTTACAGGAATAAAGGAAAAGATATTAGGACACCACGGAGCTGGATTCATATGGAAT
GCGGAGGAGTGGAAGGTTCA

Downstream 100 bases:

>100_bases
GTGCAAACATGAATAAAAAATCTTATAAAATATGGATTATAGTTTTTTCAATAATAATATTAAGTTCTGTTTTTGCACCC
ATAATAACTAAGTTTAATCC

Product: peptide/opine/nickel uptake ABC transporter permease

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MTSRYILKRLGQGILIIFFLSIISFLIINAAPGDPAVAIYGGKADRLTNSERARIVKNYGLDRPVMERYIKWSGQMIRGD
MGISYIEGRSVSEILKEKIPNTMVLFINSILLISLISMILGLKAGFNEGSIWDKLLSTLSIVFYSIPPFWLALIFILVFS
VYTGCLPSSGNMSIGGEGSVLDVLKHAILPIAVIVITHVGAYSRFIQEKVKEEDKSYYAMVARANGVLEKKIRKGITKNA
VVPFINYLGITIPTFFSGSVMIETVFSWPGLGMLTVKAANSRDYPLLMGAIFITGVLVVLCMIVTDIIEIIVNPHLRK

Sequences:

>Translated_318_residues
MTSRYILKRLGQGILIIFFLSIISFLIINAAPGDPAVAIYGGKADRLTNSERARIVKNYGLDRPVMERYIKWSGQMIRGD
MGISYIEGRSVSEILKEKIPNTMVLFINSILLISLISMILGLKAGFNEGSIWDKLLSTLSIVFYSIPPFWLALIFILVFS
VYTGCLPSSGNMSIGGEGSVLDVLKHAILPIAVIVITHVGAYSRFIQEKVKEEDKSYYAMVARANGVLEKKIRKGITKNA
VVPFINYLGITIPTFFSGSVMIETVFSWPGLGMLTVKAANSRDYPLLMGAIFITGVLVVLCMIVTDIIEIIVNPHLRK
>Mature_317_residues
TSRYILKRLGQGILIIFFLSIISFLIINAAPGDPAVAIYGGKADRLTNSERARIVKNYGLDRPVMERYIKWSGQMIRGDM
GISYIEGRSVSEILKEKIPNTMVLFINSILLISLISMILGLKAGFNEGSIWDKLLSTLSIVFYSIPPFWLALIFILVFSV
YTGCLPSSGNMSIGGEGSVLDVLKHAILPIAVIVITHVGAYSRFIQEKVKEEDKSYYAMVARANGVLEKKIRKGITKNAV
VPFINYLGITIPTFFSGSVMIETVFSWPGLGMLTVKAANSRDYPLLMGAIFITGVLVVLCMIVTDIIEIIVNPHLRK

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence, though, unlike OPP, is incapable of transportin

COG id: COG0601

COG function: function code EP; ABC-type dipeptide/oligopeptide/nickel transport systems, permease components

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789965, Length=337, Percent_Identity=26.4094955489614, Blast_Score=155, Evalue=2e-39,
Organism=Escherichia coli, GI1787053, Length=316, Percent_Identity=29.746835443038, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1788504, Length=356, Percent_Identity=30.6179775280899, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI1789888, Length=316, Percent_Identity=28.7974683544304, Blast_Score=140, Evalue=8e-35,
Organism=Escherichia coli, GI1787497, Length=316, Percent_Identity=27.2151898734177, Blast_Score=127, Evalue=7e-31,
Organism=Escherichia coli, GI1787761, Length=310, Percent_Identity=30.3225806451613, Blast_Score=127, Evalue=8e-31,
Organism=Escherichia coli, GI1787550, Length=272, Percent_Identity=27.5735294117647, Blast_Score=117, Evalue=7e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 35148; Mature: 35016

Theoretical pI: Translated: 9.94; Mature: 9.94

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSRYILKRLGQGILIIFFLSIISFLIINAAPGDPAVAIYGGKADRLTNSERARIVKNYG
CCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHCCCHHHHHHHHHCC
LDRPVMERYIKWSGQMIRGDMGISYIEGRSVSEILKEKIPNTMVLFINSILLISLISMIL
CCHHHHHHHHHHCCCEEECCCCCHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
GLKAGFNEGSIWDKLLSTLSIVFYSIPPFWLALIFILVFSVYTGCLPSSGNMSIGGEGSV
HHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHH
LDVLKHAILPIAVIVITHVGAYSRFIQEKVKEEDKSYYAMVARANGVLEKKIRKGITKNA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
VVPFINYLGITIPTFFSGSVMIETVFSWPGLGMLTVKAANSRDYPLLMGAIFITGVLVVL
HHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHH
CMIVTDIIEIIVNPHLRK
HHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TSRYILKRLGQGILIIFFLSIISFLIINAAPGDPAVAIYGGKADRLTNSERARIVKNYG
CHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHCCCHHHHHHHHHCC
LDRPVMERYIKWSGQMIRGDMGISYIEGRSVSEILKEKIPNTMVLFINSILLISLISMIL
CCHHHHHHHHHHCCCEEECCCCCHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
GLKAGFNEGSIWDKLLSTLSIVFYSIPPFWLALIFILVFSVYTGCLPSSGNMSIGGEGSV
HHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHH
LDVLKHAILPIAVIVITHVGAYSRFIQEKVKEEDKSYYAMVARANGVLEKKIRKGITKNA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
VVPFINYLGITIPTFFSGSVMIETVFSWPGLGMLTVKAANSRDYPLLMGAIFITGVLVVL
HHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHH
CMIVTDIIEIIVNPHLRK
HHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]