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The map label for this gene is pcrA [H]

Identifier: 226948601

GI number: 226948601

Start: 1547917

End: 1550484

Strand: Direct

Name: pcrA [H]

Synonym: CLM_1495

Alternate gene names: 226948601

Gene position: 1547917-1550484 (Clockwise)

Preceding gene: 226948600

Following gene: 226948605

Centisome position: 37.25

GC content: 25.16

Gene sequence:

>2568_bases
ATGTTAGATATATACCTTAATAATAAACAGAAAAAAGTAATAAATACATTAGATAAAAATATACTCCTTTTGTCTTCTGC
AGGTACAGGGAAAACAAAAACATTAAGTATGAGGATAGGAAATATAATAGCTAAAGACTTAGCTTTAGGAGAGCAAATAC
TTTGTTTAACTTTCACTAACAGAGCATGTAAGGAAATGAAAGAAAAAATAATAGAAACAGTAGGGAAAGAGGGGTTGAAA
GTTACTGTAAAGACTTTTCATAGTTTTTGTTTTGATGTAATAAAAAAAGAGGCAAAAAAGAATACGGATATTTCCTTTGA
TTTTACTATATATGATGAAGAAGATACAAAGGAAATAATAAGTGAATTAAATCCATATAAGTTTAATGTTTCTTGTCTTC
AAAGATTTATAAACATTGTTAAAGAAAAAAGTATAAATTATAAAGATAGAGATTATAAAGATGTTATAGATACCATGATA
AAATATAATACCAACATATTTAATAATATATGTAAAAATGAAAAATATATGGTAGATAAAACTTTGGTAGATTATTTAAA
AATATATGGATATCTAACGGTGATAAGCTATGATAAAAAGCTATATGAAAGGCATGGATTAGATTTTAATGATTTAATAA
TAAAGGTTCATGACTTATTTCAAAAGGATAAAATAACTACATATTGGAGAGAAAAATATAAATTTATTCATATAGATGAA
ATGCAGGATACCAGTGAAATAGAATACAATATAGTTTCAAAACTATTTAGAAATAACAATATAATGCTTAGTGGCGATTA
TTATCAGACAATATATGAATGGAGAGGTTCTAATCCTACCATAATATTTGATAATTATAAAAAGGAATTTAATCCTGAAT
TTATAGTTTTTGATAAAAACTATAGATCTTCAGAAATATTATTAAAGGCCTCATATGAATTTTTACTAAATGCTTTTGGA
AAAGGTGTAAATGAAATATATAAAGAAGGTATAGAGCCTAAAGCTAATATAAAAGGGGATTTAATAGAATTTAAGAAAGC
TTATGATTTTATGGAAGAGGCAACTTTTATATTTAATAAGATAAAATCTTTAAAGGTAGAAAAGCTTTCAAGAGTTGCTA
TATTAACTAGGAATAATAGAATAAATATAGCTTTAAGTGAAAAGTTTAAAATACTAAATTCAAAGCTTCCGAAAGAAGAG
AGAGTAGGATTCCTTTTGGTGGATGAATTTAAATTCTTTAGAAGAGAGGAAATAAAGGATGTATTAGCTTATTTAAAACT
TATAGTAAATGATTTTGATAGTAATAGTTTAAAAAGGATATTAAAGAAGTTTGGAAAGGGCATAGGAGAAAGAACCATAG
AAGAAATAGAAAAAGAGGAAAATATAAAAATAGGTGTAAGACTTACAGATTTAATTCATAAGAATACTCAAGAATATGGA
GATTATTATGAGCTTTTAACTAGAGAATTAGATAATGAAAATGTAGTAGTTTTTGATGTAGAAAGCACAGGTATCAATAT
TATAGAGGATGAAATAGTGCAAATTGCAGGCATTAAAATAAACAATAAAGGAGAAGTTATAGAAAGCTTTCAAAGATTTT
TGATTCCCAAGAAGTCTGTAGGGGATTCTTACTTAGTTCATGGTTTTAGTGACCAATTTTTAAAAGAAAATGGAGAAAAT
AAAAAGGTAGTTTTAAAAGATTTTTTAGAATTTATAAAAGATACAGTAATAGTCGGGCATAATGTAACCTTTGATATATC
CATATTAAATAGTGAACTTGAAAGATTAAGTTTAGAGAAAGCAAATTTTAAGACCTATTATGATACTTTAGATATAGCTA
GAAGATTTTATCCTAGTTTAACAAATCATAAATTAGAAACTTTAAGTAAGTTATTTAATACAGAAACGAAATCTAGCCAT
GATGCTATGGATGATATATTGGCTACAAAAGATGTTCTTATGGCTATGCTAAAGGAAAAAGTTAAACCTACTATAATAAA
TAGAATGGTAGTGTACGGGAAATATATTAAAAAATTTGAACCTATATATGAAGAAATAACTAATTTAAAAAAATTAAGTT
ATATAAAAAATCCAAAGGACATAATAGGAGAAATAGTTAATAAAACGAAAATAAAGGAAATATACAAAGACCAATATATT
AAGATAAACAATCTGAGGGAATTTTTTGTTATAGCTAAAGAAATAGAAAATAAAGAAATCTCATCAAGAGATAACTTAAT
AGAACTTTTAAAAATAACTTCTTTGTCTAATAGCGAATTAGATAGAATGCTTACAAAATATCCAAGAGTACCAATAATAA
CAGTGCATCAAGCAAAGGGTGCGGAATTTGATTATGTATTTTTAGCTGGACTTCAAAATAATATATTTCCAAATTACATG
GCAGTAAAAGAAGGAAATGAAATAGAAGAGAGAAGACTATTTTATGTCGCTATGACTAGAGCAAAAAAATATTTATATCT
AAGCTTTTCAGAATATGAGCATAACAGAAGAAAAGCTAAAAGTATGCTTATAGATTATATACCTAAAGATTATTTAAAGT
ATATGTAA

Upstream 100 bases:

>100_bases
CAATTTTTTTGAGTTAACTTTACAAAAAGCAAAGAATCACTTGAAAATAAATTAAATATATTTAATAATAAAAAGCAAGG
TATAATTGGAGGTGAATCTT

Downstream 100 bases:

>100_bases
TAAATATAAGTTAAAGCCACACTAAGAAAAATATAATTTAAATATTTTTACTTAATGTGGCTTTCTTATAGAATAATGCC
TATAGACATTATATTCCTCT

Product: helicase, UvrD/REP/exonuclease family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 855; Mature: 855

Protein sequence:

>855_residues
MLDIYLNNKQKKVINTLDKNILLLSSAGTGKTKTLSMRIGNIIAKDLALGEQILCLTFTNRACKEMKEKIIETVGKEGLK
VTVKTFHSFCFDVIKKEAKKNTDISFDFTIYDEEDTKEIISELNPYKFNVSCLQRFINIVKEKSINYKDRDYKDVIDTMI
KYNTNIFNNICKNEKYMVDKTLVDYLKIYGYLTVISYDKKLYERHGLDFNDLIIKVHDLFQKDKITTYWREKYKFIHIDE
MQDTSEIEYNIVSKLFRNNNIMLSGDYYQTIYEWRGSNPTIIFDNYKKEFNPEFIVFDKNYRSSEILLKASYEFLLNAFG
KGVNEIYKEGIEPKANIKGDLIEFKKAYDFMEEATFIFNKIKSLKVEKLSRVAILTRNNRINIALSEKFKILNSKLPKEE
RVGFLLVDEFKFFRREEIKDVLAYLKLIVNDFDSNSLKRILKKFGKGIGERTIEEIEKEENIKIGVRLTDLIHKNTQEYG
DYYELLTRELDNENVVVFDVESTGINIIEDEIVQIAGIKINNKGEVIESFQRFLIPKKSVGDSYLVHGFSDQFLKENGEN
KKVVLKDFLEFIKDTVIVGHNVTFDISILNSELERLSLEKANFKTYYDTLDIARRFYPSLTNHKLETLSKLFNTETKSSH
DAMDDILATKDVLMAMLKEKVKPTIINRMVVYGKYIKKFEPIYEEITNLKKLSYIKNPKDIIGEIVNKTKIKEIYKDQYI
KINNLREFFVIAKEIENKEISSRDNLIELLKITSLSNSELDRMLTKYPRVPIITVHQAKGAEFDYVFLAGLQNNIFPNYM
AVKEGNEIEERRLFYVAMTRAKKYLYLSFSEYEHNRRKAKSMLIDYIPKDYLKYM

Sequences:

>Translated_855_residues
MLDIYLNNKQKKVINTLDKNILLLSSAGTGKTKTLSMRIGNIIAKDLALGEQILCLTFTNRACKEMKEKIIETVGKEGLK
VTVKTFHSFCFDVIKKEAKKNTDISFDFTIYDEEDTKEIISELNPYKFNVSCLQRFINIVKEKSINYKDRDYKDVIDTMI
KYNTNIFNNICKNEKYMVDKTLVDYLKIYGYLTVISYDKKLYERHGLDFNDLIIKVHDLFQKDKITTYWREKYKFIHIDE
MQDTSEIEYNIVSKLFRNNNIMLSGDYYQTIYEWRGSNPTIIFDNYKKEFNPEFIVFDKNYRSSEILLKASYEFLLNAFG
KGVNEIYKEGIEPKANIKGDLIEFKKAYDFMEEATFIFNKIKSLKVEKLSRVAILTRNNRINIALSEKFKILNSKLPKEE
RVGFLLVDEFKFFRREEIKDVLAYLKLIVNDFDSNSLKRILKKFGKGIGERTIEEIEKEENIKIGVRLTDLIHKNTQEYG
DYYELLTRELDNENVVVFDVESTGINIIEDEIVQIAGIKINNKGEVIESFQRFLIPKKSVGDSYLVHGFSDQFLKENGEN
KKVVLKDFLEFIKDTVIVGHNVTFDISILNSELERLSLEKANFKTYYDTLDIARRFYPSLTNHKLETLSKLFNTETKSSH
DAMDDILATKDVLMAMLKEKVKPTIINRMVVYGKYIKKFEPIYEEITNLKKLSYIKNPKDIIGEIVNKTKIKEIYKDQYI
KINNLREFFVIAKEIENKEISSRDNLIELLKITSLSNSELDRMLTKYPRVPIITVHQAKGAEFDYVFLAGLQNNIFPNYM
AVKEGNEIEERRLFYVAMTRAKKYLYLSFSEYEHNRRKAKSMLIDYIPKDYLKYM
>Mature_855_residues
MLDIYLNNKQKKVINTLDKNILLLSSAGTGKTKTLSMRIGNIIAKDLALGEQILCLTFTNRACKEMKEKIIETVGKEGLK
VTVKTFHSFCFDVIKKEAKKNTDISFDFTIYDEEDTKEIISELNPYKFNVSCLQRFINIVKEKSINYKDRDYKDVIDTMI
KYNTNIFNNICKNEKYMVDKTLVDYLKIYGYLTVISYDKKLYERHGLDFNDLIIKVHDLFQKDKITTYWREKYKFIHIDE
MQDTSEIEYNIVSKLFRNNNIMLSGDYYQTIYEWRGSNPTIIFDNYKKEFNPEFIVFDKNYRSSEILLKASYEFLLNAFG
KGVNEIYKEGIEPKANIKGDLIEFKKAYDFMEEATFIFNKIKSLKVEKLSRVAILTRNNRINIALSEKFKILNSKLPKEE
RVGFLLVDEFKFFRREEIKDVLAYLKLIVNDFDSNSLKRILKKFGKGIGERTIEEIEKEENIKIGVRLTDLIHKNTQEYG
DYYELLTRELDNENVVVFDVESTGINIIEDEIVQIAGIKINNKGEVIESFQRFLIPKKSVGDSYLVHGFSDQFLKENGEN
KKVVLKDFLEFIKDTVIVGHNVTFDISILNSELERLSLEKANFKTYYDTLDIARRFYPSLTNHKLETLSKLFNTETKSSH
DAMDDILATKDVLMAMLKEKVKPTIINRMVVYGKYIKKFEPIYEEITNLKKLSYIKNPKDIIGEIVNKTKIKEIYKDQYI
KINNLREFFVIAKEIENKEISSRDNLIELLKITSLSNSELDRMLTKYPRVPIITVHQAKGAEFDYVFLAGLQNNIFPNYM
AVKEGNEIEERRLFYVAMTRAKKYLYLSFSEYEHNRRKAKSMLIDYIPKDYLKYM

Specific function: DNA helicase. Has a broad nucleotide specificity, even being able to hydrolyze ethenonucleotides, and is able to couple the hydrolysis to unwinding of DNA substrates. It is a 3'-5' helicase but at high protein concentrations it can also displace a substra

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=462, Percent_Identity=27.0562770562771, Blast_Score=177, Evalue=2e-45,
Organism=Escherichia coli, GI48994965, Length=470, Percent_Identity=26.1702127659574, Blast_Score=133, Evalue=5e-32,
Organism=Escherichia coli, GI1787196, Length=314, Percent_Identity=23.2484076433121, Blast_Score=85, Evalue=2e-17,
Organism=Escherichia coli, GI1786409, Length=172, Percent_Identity=27.906976744186, Blast_Score=65, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6322369, Length=487, Percent_Identity=24.8459958932238, Blast_Score=101, Evalue=6e-22,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 100598; Mature: 100598

Theoretical pI: Translated: 8.96; Mature: 8.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDIYLNNKQKKVINTLDKNILLLSSAGTGKTKTLSMRIGNIIAKDLALGEQILCLTFTN
CEEEEECCCHHHHHHHHCCCEEEEECCCCCCCEEEEEHHHHHHHHHHHCCCEEEEEEECC
RACKEMKEKIIETVGKEGLKVTVKTFHSFCFDVIKKEAKKNTDISFDFTIYDEEDTKEII
HHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHH
SELNPYKFNVSCLQRFINIVKEKSINYKDRDYKDVIDTMIKYNTNIFNNICKNEKYMVDK
HHCCCCEEEHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHHCCCCEEHHH
TLVDYLKIYGYLTVISYDKKLYERHGLDFNDLIIKVHDLFQKDKITTYWREKYKFIHIDE
HHHHHHHHHHHHHEEHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHCCEEEEEEC
MQDTSEIEYNIVSKLFRNNNIMLSGDYYQTIYEWRGSNPTIIFDNYKKEFNPEFIVFDKN
CCCCHHHHHHHHHHHHCCCCEEEECHHHHHHHHHCCCCCEEEECCCCCCCCCCEEEEECC
YRSSEILLKASYEFLLNAFGKGVNEIYKEGIEPKANIKGDLIEFKKAYDFMEEATFIFNK
CCCCCEEEEHHHHHHHHHHCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
IKSLKVEKLSRVAILTRNNRINIALSEKFKILNSKLPKEERVGFLLVDEFKFFRREEIKD
HHHHHHHHHHEEEEEEECCEEEEEEHHHHHHHHCCCCHHHCCCEEEEHHHHHHHHHHHHH
VLAYLKLIVNDFDSNSLKRILKKFGKGIGERTIEEIEKEENIKIGVRLTDLIHKNTQEYG
HHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCCCEEEEEEHHHHHHCHHHHH
DYYELLTRELDNENVVVFDVESTGINIIEDEIVQIAGIKINNKGEVIESFQRFLIPKKSV
HHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHEEECEEECCCCHHHHHHHHHCCCCCCC
GDSYLVHGFSDQFLKENGENKKVVLKDFLEFIKDTVIVGHNVTFDISILNSELERLSLEK
CCCCEEECCCHHHHHCCCCCCEEHHHHHHHHHHHHEEECCCEEEEEEEHHHHHHHHHHHH
ANFKTYYDTLDIARRFYPSLTNHKLETLSKLFNTETKSSHDAMDDILATKDVLMAMLKEK
CCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
VKPTIINRMVVYGKYIKKFEPIYEEITNLKKLSYIKNPKDIIGEIVNKTKIKEIYKDQYI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCE
KINNLREFFVIAKEIENKEISSRDNLIELLKITSLSNSELDRMLTKYPRVPIITVHQAKG
EECCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEECCCC
AEFDYVFLAGLQNNIFPNYMAVKEGNEIEERRLFYVAMTRAKKYLYLSFSEYEHNRRKAK
CCCCEEEEECCCCCCCCCEEEECCCCCHHHHHHHHEEEHHCCEEEEEEEHHHHHHHHHHH
SMLIDYIPKDYLKYM
HHHHHHCCHHHHHCC
>Mature Secondary Structure
MLDIYLNNKQKKVINTLDKNILLLSSAGTGKTKTLSMRIGNIIAKDLALGEQILCLTFTN
CEEEEECCCHHHHHHHHCCCEEEEECCCCCCCEEEEEHHHHHHHHHHHCCCEEEEEEECC
RACKEMKEKIIETVGKEGLKVTVKTFHSFCFDVIKKEAKKNTDISFDFTIYDEEDTKEII
HHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHH
SELNPYKFNVSCLQRFINIVKEKSINYKDRDYKDVIDTMIKYNTNIFNNICKNEKYMVDK
HHCCCCEEEHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHHCCCCEEHHH
TLVDYLKIYGYLTVISYDKKLYERHGLDFNDLIIKVHDLFQKDKITTYWREKYKFIHIDE
HHHHHHHHHHHHHEEHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHCCEEEEEEC
MQDTSEIEYNIVSKLFRNNNIMLSGDYYQTIYEWRGSNPTIIFDNYKKEFNPEFIVFDKN
CCCCHHHHHHHHHHHHCCCCEEEECHHHHHHHHHCCCCCEEEECCCCCCCCCCEEEEECC
YRSSEILLKASYEFLLNAFGKGVNEIYKEGIEPKANIKGDLIEFKKAYDFMEEATFIFNK
CCCCCEEEEHHHHHHHHHHCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
IKSLKVEKLSRVAILTRNNRINIALSEKFKILNSKLPKEERVGFLLVDEFKFFRREEIKD
HHHHHHHHHHEEEEEEECCEEEEEEHHHHHHHHCCCCHHHCCCEEEEHHHHHHHHHHHHH
VLAYLKLIVNDFDSNSLKRILKKFGKGIGERTIEEIEKEENIKIGVRLTDLIHKNTQEYG
HHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCCCEEEEEEHHHHHHCHHHHH
DYYELLTRELDNENVVVFDVESTGINIIEDEIVQIAGIKINNKGEVIESFQRFLIPKKSV
HHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHEEECEEECCCCHHHHHHHHHCCCCCCC
GDSYLVHGFSDQFLKENGENKKVVLKDFLEFIKDTVIVGHNVTFDISILNSELERLSLEK
CCCCEEECCCHHHHHCCCCCCEEHHHHHHHHHHHHEEECCCEEEEEEEHHHHHHHHHHHH
ANFKTYYDTLDIARRFYPSLTNHKLETLSKLFNTETKSSHDAMDDILATKDVLMAMLKEK
CCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
VKPTIINRMVVYGKYIKKFEPIYEEITNLKKLSYIKNPKDIIGEIVNKTKIKEIYKDQYI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCE
KINNLREFFVIAKEIENKEISSRDNLIELLKITSLSNSELDRMLTKYPRVPIITVHQAKG
EECCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEECCCC
AEFDYVFLAGLQNNIFPNYMAVKEGNEIEERRLFYVAMTRAKKYLYLSFSEYEHNRRKAK
CCCCEEEEECCCCCCCCCEEEECCCCCHHHHHHHHEEEHHCCEEEEEEEHHHHHHHHHHH
SMLIDYIPKDYLKYM
HHHHHHCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9592155; 8934527; 10199404; 10388562; 10454638 [H]