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The map label for this gene is ykoW [H]

Identifier: 226948480

GI number: 226948480

Start: 1431775

End: 1433034

Strand: Direct

Name: ykoW [H]

Synonym: CLM_1368

Alternate gene names: 226948480

Gene position: 1431775-1433034 (Clockwise)

Preceding gene: 226948479

Following gene: 226948481

Centisome position: 34.46

GC content: 25.63

Gene sequence:

>1260_bases
ATGAAAGAGAAAAATTTAATAAAACAAATGGAGTTATCTGTTATAGAAAAAATGGGTATAGCTTATTGTTGCTGCAAAAT
AAGTTTGAACTCATATGGTCAGTTAGAGGATTTTATTTTTATGGAATCTAATGATTATTTTAAAGATTTAACGGGTTTTA
CAAAAGAATATATAAAAAATAAAAAAGCTAAAGATATAATTGCCTTTGGAAGTCATAGAATAAATATAATGAAAGCCTTA
GAAAAAATTTTATTATATAAAGAAGAAAAAATAGAATTTGAAGACTATTTTGATATTACCAAAAAATGGGTTAAAGTATT
ATTGTATACACAAAAAAAACAGGTTTTTTTTATCACATTTTATGATATAACCAAATATAAAGTCAAAGAAATAGAATTGA
AAAATACAATTAAAAAATACAAATATCTTATAGAGTTATCTGCGGACGCAATAATAATTAGAAATTTAAATGGGGATATA
ATATATTGTAACAAATCTGCTCTTAATCTGTTTGGATATTCCTTGGAAGAAATGAAAAATCTAAATATGATAGATTTAGT
CCCTAGATCAGTTATGAAAGATATAGGGTTTAATATGTCCATAGGAGATAAGCCTGTTGAGAGAGTACATAAAAGAAAAG
ATGGAACACATTTTTATGGGGAGGAAACAACAAAATTAATAAATATAGAAGGGGAATTGGGAATTGCAACATATATAAGG
GATATAACAGAACGGAAAATATATAATGATAAAACGAAACAAATGGCTTATTTTGACTCTCTTACAGAGTTACCTAATAG
AAACTCTTTCTTAAAACAATTAGAGCATGAAATAAAATTAAGCAAGAAAAAACAAAGCTTATTGGCTATAATGTTTCTAG
ATCTTGATAAATTTAAGGAAGTAAATGATAATTTTGGTCATTGCACTGGGGATAAACTTTTATGGCAAGTAGCTAAAAGG
GTAAAGAATACTATTAGTTCTAAAGATTTAATAGCTAGATTTGGAGGAGATGAATTTACCATATTAATAAGAAATATAGC
TCACGAAAAACAAGTTGAAGATTTAGCTATGGATATAATAGAGGTCTTTAAAGAACCTATATGTATAGAAGAAATATATG
TAAATATAAAGACTAGTATAGGTATAAGCTTTTTTCCAAAAGATGGCCATACATCACAAGAACTAATAAAAAAAGCAGAT
AAAGCTATGTATGATGCTAAAAAAAGAGGGAGTAATAAATTTCAAATATATAAAGATTAA

Upstream 100 bases:

>100_bases
AGTTATTGGGGAAAATTCCTTAATTATAAATACTTATTTCAAGCTATAGGGAATATATTAATAGTATATCAATATATAAC
TGAACATAAGAGGTGAAACT

Downstream 100 bases:

>100_bases
AAAAGTATTGTGTAAAGCTTAAATATAATAATTTAATATTTTTAATAAAAATTAGGGATGTATTTATATAGATGATAGGT
ATAGTATCTATATAAATATA

Product: putative sensory box-containing diguanylate cyclase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 419; Mature: 419

Protein sequence:

>419_residues
MKEKNLIKQMELSVIEKMGIAYCCCKISLNSYGQLEDFIFMESNDYFKDLTGFTKEYIKNKKAKDIIAFGSHRINIMKAL
EKILLYKEEKIEFEDYFDITKKWVKVLLYTQKKQVFFITFYDITKYKVKEIELKNTIKKYKYLIELSADAIIIRNLNGDI
IYCNKSALNLFGYSLEEMKNLNMIDLVPRSVMKDIGFNMSIGDKPVERVHKRKDGTHFYGEETTKLINIEGELGIATYIR
DITERKIYNDKTKQMAYFDSLTELPNRNSFLKQLEHEIKLSKKKQSLLAIMFLDLDKFKEVNDNFGHCTGDKLLWQVAKR
VKNTISSKDLIARFGGDEFTILIRNIAHEKQVEDLAMDIIEVFKEPICIEEIYVNIKTSIGISFFPKDGHTSQELIKKAD
KAMYDAKKRGSNKFQIYKD

Sequences:

>Translated_419_residues
MKEKNLIKQMELSVIEKMGIAYCCCKISLNSYGQLEDFIFMESNDYFKDLTGFTKEYIKNKKAKDIIAFGSHRINIMKAL
EKILLYKEEKIEFEDYFDITKKWVKVLLYTQKKQVFFITFYDITKYKVKEIELKNTIKKYKYLIELSADAIIIRNLNGDI
IYCNKSALNLFGYSLEEMKNLNMIDLVPRSVMKDIGFNMSIGDKPVERVHKRKDGTHFYGEETTKLINIEGELGIATYIR
DITERKIYNDKTKQMAYFDSLTELPNRNSFLKQLEHEIKLSKKKQSLLAIMFLDLDKFKEVNDNFGHCTGDKLLWQVAKR
VKNTISSKDLIARFGGDEFTILIRNIAHEKQVEDLAMDIIEVFKEPICIEEIYVNIKTSIGISFFPKDGHTSQELIKKAD
KAMYDAKKRGSNKFQIYKD
>Mature_419_residues
MKEKNLIKQMELSVIEKMGIAYCCCKISLNSYGQLEDFIFMESNDYFKDLTGFTKEYIKNKKAKDIIAFGSHRINIMKAL
EKILLYKEEKIEFEDYFDITKKWVKVLLYTQKKQVFFITFYDITKYKVKEIELKNTIKKYKYLIELSADAIIIRNLNGDI
IYCNKSALNLFGYSLEEMKNLNMIDLVPRSVMKDIGFNMSIGDKPVERVHKRKDGTHFYGEETTKLINIEGELGIATYIR
DITERKIYNDKTKQMAYFDSLTELPNRNSFLKQLEHEIKLSKKKQSLLAIMFLDLDKFKEVNDNFGHCTGDKLLWQVAKR
VKNTISSKDLIARFGGDEFTILIRNIAHEKQVEDLAMDIIEVFKEPICIEEIYVNIKTSIGISFFPKDGHTSQELIKKAD
KAMYDAKKRGSNKFQIYKD

Specific function: Probable signaling protein whose physiological role is not yet known [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1787541, Length=177, Percent_Identity=35.5932203389831, Blast_Score=119, Evalue=5e-28,
Organism=Escherichia coli, GI87081881, Length=315, Percent_Identity=28.5714285714286, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1788381, Length=283, Percent_Identity=27.9151943462898, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI145693134, Length=178, Percent_Identity=31.4606741573034, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1787262, Length=172, Percent_Identity=31.9767441860465, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI87082007, Length=187, Percent_Identity=27.2727272727273, Blast_Score=85, Evalue=9e-18,
Organism=Escherichia coli, GI1787816, Length=182, Percent_Identity=29.6703296703297, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1786584, Length=172, Percent_Identity=26.1627906976744, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1788956, Length=167, Percent_Identity=29.940119760479, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1787802, Length=181, Percent_Identity=30.3867403314917, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI87081977, Length=114, Percent_Identity=36.8421052631579, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787056, Length=156, Percent_Identity=32.6923076923077, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1788085, Length=168, Percent_Identity=27.9761904761905, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI87081974, Length=87, Percent_Identity=39.0804597701149, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR001633
- InterPro:   IPR005330
- InterPro:   IPR000014 [H]

Pfam domain/function: PF00563 EAL; PF00990 GGDEF; PF03707 MHYT [H]

EC number: NA

Molecular weight: Translated: 49100; Mature: 49100

Theoretical pI: Translated: 9.15; Mature: 9.15

Prosite motif: PS50112 PAS ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEKNLIKQMELSVIEKMGIAYCCCKISLNSYGQLEDFIFMESNDYFKDLTGFTKEYIKN
CCCHHHHHHHHHHHHHHHCHHEEEEEEECCCCCCCCCEEEECCCCHHHHHHHHHHHHHCC
KKAKDIIAFGSHRINIMKALEKILLYKEEKIEFEDYFDITKKWVKVLLYTQKKQVFFITF
CCCCHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEE
YDITKYKVKEIELKNTIKKYKYLIELSADAIIIRNLNGDIIYCNKSALNLFGYSLEEMKN
EECCHHHHHHHHHHHHHHHHHHHEEECCCEEEEEECCCCEEEECCHHHHHHCCCHHHHCC
LNMIDLVPRSVMKDIGFNMSIGDKPVERVHKRKDGTHFYGEETTKLINIEGELGIATYIR
CCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCEECCCCCEEEEEECCCCHHHHHHH
DITERKIYNDKTKQMAYFDSLTELPNRNSFLKQLEHEIKLSKKKQSLLAIMFLDLDKFKE
HHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VNDNFGHCTGDKLLWQVAKRVKNTISSKDLIARFGGDEFTILIRNIAHEKQVEDLAMDII
HCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHH
EVFKEPICIEEIYVNIKTSIGISFFPKDGHTSQELIKKADKAMYDAKKRGSNKFQIYKD
HHHHCCCEEEHHEEEEEEECCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEC
>Mature Secondary Structure
MKEKNLIKQMELSVIEKMGIAYCCCKISLNSYGQLEDFIFMESNDYFKDLTGFTKEYIKN
CCCHHHHHHHHHHHHHHHCHHEEEEEEECCCCCCCCCEEEECCCCHHHHHHHHHHHHHCC
KKAKDIIAFGSHRINIMKALEKILLYKEEKIEFEDYFDITKKWVKVLLYTQKKQVFFITF
CCCCHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEE
YDITKYKVKEIELKNTIKKYKYLIELSADAIIIRNLNGDIIYCNKSALNLFGYSLEEMKN
EECCHHHHHHHHHHHHHHHHHHHEEECCCEEEEEECCCCEEEECCHHHHHHCCCHHHHCC
LNMIDLVPRSVMKDIGFNMSIGDKPVERVHKRKDGTHFYGEETTKLINIEGELGIATYIR
CCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCEECCCCCEEEEEECCCCHHHHHHH
DITERKIYNDKTKQMAYFDSLTELPNRNSFLKQLEHEIKLSKKKQSLLAIMFLDLDKFKE
HHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VNDNFGHCTGDKLLWQVAKRVKNTISSKDLIARFGGDEFTILIRNIAHEKQVEDLAMDII
HCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHH
EVFKEPICIEEIYVNIKTSIGISFFPKDGHTSQELIKKADKAMYDAKKRGSNKFQIYKD
HHHHCCCEEEHHEEEEEEECCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 11728710 [H]